Apollonious Identity in Linear 2-Normed Spaces

International Conference on Numerical Analysis and Applied Mathematics -- SEP 18-22, 2009 -- Rethymnon, GREECEWOS: 000273023600043We shall extend the well known Apollonious identity in functional analysis to linear 2-normed spaces and prove it in this spaces.Greek Minist Educ & Religious Affairs, European Soc Computat Methods Sci & Eng

ApoUonious Identity in Linear 2-Normed Spaces

Greek Ministry of Education and Religious Affairs;European Society of Computational;Methods in Sciences and Engineering (ESCMSE)International Conference on Numerical Analysis and Applied Mathematics 2009, ICNAAM-2009 --18 September 2009 through 22 September 2009 -- Rethymno, Crete --We shall extend the well known ApoUonious identity in functional analysis to linear 2-normed spaces and prove it in this spaces. © 2009 American Institute of Physics

Molecular surveillance of Toxoplasma gondii in raw milk and Artisan cheese of sheep, goat, cow and water buffalo origin

This study is aimed at investigating the molecular prevalence of Toxoplasma gondii in raw milk and cheese of different animal species (sheep, goat, cow and water buffalo) in Kayseri Province, Türkiye, to provide a preliminary assessment for contamination risk. A total of 200 milk and cheese samples were analysed by real-time PCR. Toxoplasma gondii DNA was detected in two (8%) ewes and one (4%) goat raw milk sample, while none of the cheese samples were positive. These results indicated that the presence of T. gondii DNA in raw milk samples sold in Kayseri Province might be a risk factor for public health

From cattle to pastirma: Contamination source of methicillin susceptible and resistant Staphylococcus aureus (MRSA) along the pastirma production chain

This study was designed to determine the prevalence of Methicillin Susceptible S. aureus (MSSA) and MethicillinResistant S. aureus (MRSA) and their source of contamination in the pastirma production chain. Additionally, this study was focused on the antimicrobial resistance, virulence profiles, biofilm-forming capabilities and phylogenetic relationships of obtained isolates. A total of 400 samples were analyzed and from which 105 (26.25%) were found positive for coagulase-positive with Staphylococci (CPS). Within the 105 CPS samples, 36 (9%) were identified as S. aureus, from which 8 (2%) were MRSA. Four (11.1%) of 36 S. aureus isolates, of which 3 (37.5%) were MRSA, had a multidrug resistance (MDR), and 6 MRSA strains were found positive for one or more SEs genes (seb, sed, and see). According to the ERIC-PCR analysis, only two S. aureus strains (one with personnel origin and one with carcass origin) were genetically identical. This study highlights the detection frequency of S. aureus in samples analyzed observed, while low, can be a significant public health problem, especially due to the identification of MRSA harboring some enterotoxin genes and having MDR

Four novel bacteriophages from slaughterhouse: Their potency on control of biofilm-forming MDR S. aureus in beef model

This study was designed to isolate and characterize novel lytic phages specific to Staphylococcus aureus (S. aureus) from wastewater samples, determine their antimicrobial and antibiofilm activities, and investigate the biocontrol efficiency of phages on raw beef contaminated with S. aureus. For this purpose, a total of 50 wastewaters from slaughterhouses were used as material, and phages were isolated with the double-layer agar method. The isolated and purified phages were characterized by whole genome sequence (WGS). Antibiofilm activity of individual phages and their cocktails (B2-102, T2-102, O1-102, and O1-69) was assessed by microplate titer plate. Five novel bacteriophages isolated with broad lytic activity against many S. aureus and E. coli strains. Four phages (B2-102, O1-102, T2-102, and O2-92) isolated belonged to the Herelleviridae family into the order Caudovirales while the remaining phage O1-69 belonged to the Siphoviridae family. No antibiotic resistance or virulence genes were determined in the isolated phages' genome. Results revealed that individual phages reduced the pre-formed mature biofilm structure by their host strains and S. epidermidis. The treatment of the individual phages and their cocktails on beef provided favorable results with a significant decrease in viable S. aureus count. Log reduction of viable S. aureus cell counts ranged from 2.00 to 3.3 CFU/g for individual phages, and from 3.1 to 4.2 cfu/g for cocktails. In conclusion, the characteristics of novel phages isolated provide strong evidence for making them a good candidate for reducing and controlling S. aureus in the food industry

<i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> in human stool samples: antibiotic resistance profiles, putative virulence determinants and molecular characterization of the isolates

Campylobacters, especially C. jejuni and C. coli, have become one of the leading causes of acute gastroenteritis in humans worldwide in recent years. We aimed to investigate the presence, antimicrobial resistance, putative virulence genes, and molecular characterization of C. jejuni and C. coli recovered from human acute gastroenteritis cases in the study. In the study, suspected Campylobacter spp. isolates were obtained in 43 (5%) feces samples collected from a total of 850 patients who applied to the Erciyes University Medical Faculty acute clinic between March 2019 and February 2020. As a result of the phenotypic tests, these isolates were determined to be Campylobacter spp. According to the multiplex PCR, 33 of 43 Campylobacter spp. isolates were identified as C. jejuni (76%) and ten isolates were as C. coli (24%). In the disc diffusion test, the highest resistance rate was found in the trimethoprim/sulfamethoxazole (90.1%) and ciprofloxacin (90.1%), and the lowest rate was found in the amoxicillin-clavulanic acid (9.3%). In Campylobacter spp. isolates, the virulence genes cdtA, virB11, cdtB, cadF, iam, ceu, and flaA were found to be positive at rates of 26 (60%), 28 (65.6%), 13 (30%), 4 (9%), 27 (62%), 17 (39%), and 7 (16%), respectively. However, the cdtC gene was not detected in any of the isolates. The study searched tetO gene to examine the genetic aspect of tetracycline resistance, which was found in all Campylobacter spp. isolates. In the PCR reactions to investigate A2074C and A2075G mutations of macrolide resistance, it was determined as 7 (16%) and 21 (48%) of the isolates. To detect quinolone resistance, the rates of quinolone resistance-determining regions (QRDR) were 20 (45.4%) and the gyrA gene mutations in the mismatch amplification mutation assay PCR (MAMA-PCR), were 19 (43.1%) of isolates. In addition, the quinolone resistance gene (qnr) carried by plasmid in Campylobacter isolates was not found in the study. BlaOXA-61 and CmeB (multi-drug efflux pump) genes were detected as 28 (63.6%) and 30 (68.1), respectively. The Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) used for typing the isolates revealed that the band profiles obtained from the isolates were different. In conclusion, this was a very comprehensive study revealing the presence of antibiotic-resistant C. jejuni and C. coli with various virulence genes in patients admitted to a university hospital with acute gastroenteritis. This is of utmost significance for public health. Since campylobacteria are foodborne, zoonotic pathogens and transmission occurs mostly through food. People should have detailed information about the transmission routes of campylobacteria and risky foods. In addition, staff, food processors and caterers, should be trained in hygiene