Comparison of fecal indicators with pathogenic bacteria and rotavirus in groundwater

Groundwater is routinely analyzed for fecal indicators but direct comparisons of fecal indicators to the presence of bacterial and viral pathogens are rare. This study was conducted in rural Bangladesh where the human population density is high, sanitation is poor, and groundwater pumped from shallow tubewells is often contaminated with fecal bacteria. Five indicator microorganisms (E. coli, total coliform, F+RNA coliphage, Bacteroides and human-associated Bacteroides) and various environmental parameters were compared to the direct detection of waterborne pathogens by quantitative PCR in groundwater pumped from 50 tubewells. Rotavirus was detected in groundwater filtrate from the largest proportion of tubewells (40%), followed by Shigella (10%), Vibrio (10%), and pathogenic E. coli (8%). Spearman rank correlations and sensitivity-specificity calculations indicate that some, but not all, combinations of indicators and environmental parameters can predict the presence of pathogens. Culture-dependent fecal indicator bacteria measured on a single date did not predict total bacterial pathogens, but annually averaged monthly measurements of culturable E. coli did improve prediction for total bacterial pathogens. A qPCR-based E. coli assay was the best indicator for the bacterial pathogens. F+RNA coliphage were neither correlated nor sufficiently sensitive towards rotavirus, but were predictive of bacterial pathogens. Since groundwater cannot be excluded as a significant source of diarrheal disease in Bangladesh and neighboring countries with similar characteristics, the need to develop more effective methods for screening tubewells with respect to microbial contamination is necessary

Minoan Archaeology

More than 100 years ago Sir Arthur Evans' spade made the first cut into the earth above the now well-known Palace at Knossos. His research at the Kephala hill as well as contemporary fieldwork at further sites on Crete saw the birth of a new discipline: Minoan Archaeology. Since these beginnings in the first decades of the 20th century, the investigation of Bronze Age Crete has experienced fundamental progress. The impressive wealth of new data relating to the sites and material culture of this Bronze Age society and its impact beyond the island's shores, the refinement of its chronology, the constant development of hermeneutical approaches to social, religious or political issues, and new methods and instruments employed for the exploration and conservation of the archaeological remains have shaped the dynamic trajectory of this discipline for more than a century. In March 2011 - exactly 111 years after the beginning of Evans' work at Knossos - a conference on Minoan Archaeology took place at Heidelberg with the aim to outline current trends and prospects of this scientific field, by setting up an open dialogue between renowned scholars and the young generation of researchers. The present volume brings together most of the papers presented during the conference. They are subsumed under six chapters highlighting current key issues in the study of Bronze Age Crete with a pronounced focus on the broad subject of society

Minoan Archaeology

More than 100 years ago Sir Arthur Evans' spade made the first cut into the earth above the now well-known Palace at Knossos. His research at the Kephala hill as well as contemporary fieldwork at further sites on Crete saw the birth of a new discipline: Minoan Archaeology. Since these beginnings in the first decades of the 20th century, the investigation of Bronze Age Crete has experienced fundamental progress. The impressive wealth of new data relating to the sites and material culture of this Bronze Age society and its impact beyond the island's shores, the refinement of its chronology, the constant development of hermeneutical approaches to social, religious or political issues, and new methods and instruments employed for the exploration and conservation of the archaeological remains have shaped the dynamic trajectory of this discipline for more than a century. In March 2011 - exactly 111 years after the beginning of Evans' work at Knossos - a conference on Minoan Archaeology took place at Heidelberg with the aim to outline current trends and prospects of this scientific field, by setting up an open dialogue between renowned scholars and the young generation of researchers. The present volume brings together most of the papers presented during the conference. They are subsumed under six chapters highlighting current key issues in the study of Bronze Age Crete with a pronounced focus on the broad subject of society

Comparison of fecal indicators with pathogenic bacteria and rotavirus in groundwater

Groundwater is routinely analyzed for fecal indicators but direct comparisons of fecal indicators to the presence of bacterial and viral pathogens are rare. This study was conducted in rural Bangladesh where the human population density is high, sanitation is poor, and groundwater pumped from shallow tubewells is often contaminated with fecal bacteria. Five indicator microorganisms (E. coli, total coliform, F+RNA coliphage, Bacteroides and human-associated Bacteroides) and various environmental parameters were compared to the direct detection of waterborne pathogens by quantitative PCR in groundwater pumped from 50 tubewells. Rotavirus was detected in groundwater filtrate from the largest proportion of tubewells (40%), followed by Shigella (10%), Vibrio (10%), and pathogenic E. coli (8%). Spearman rank correlations and sensitivity-specificity calculations indicate that some, but not all, combinations of indicators and environmental parameters can predict the presence of pathogens. Culture-dependent fecal indicator bacteria measured on a single date did not predict total bacterial pathogens, but annually averaged monthly measurements of culturable E. coli did improve prediction for total bacterial pathogens. A qPCR-based E. coli assay was the best indicator for the bacterial pathogens. F+RNA coliphage were neither correlated nor sufficiently sensitive towards rotavirus, but were predictive of bacterial pathogens. Since groundwater cannot be excluded as a significant source of diarrheal disease in Bangladesh and neighboring countries with similar characteristics, the need to develop more effective methods for screening tubewells with respect to microbial contamination is necessary