Habitat Harz: Paläobotanische Untersuchungen zur Umweltgeschichte eines Mittelgebirges

Die Dissertation wurde an der Graduiertenschule “Human development in Landscapes” der Christian-Albrechts-Universität zu Kiel angefertigt. Ihm Rahmen des Dissertationsprojektes wurden im Harz anthropogene und natürliche Archive (Moore, Meilerplätze und archäologische Fundstätten) paläobotanisch untersucht (Pollenanalyse, Holzkohleanalyse, Makrorestanalyse). Ziel des für den Harz neuen Methodenverbundes ist es, die regionale und lokale Umwelt- und Waldgeschichte des Harzes zu rekonstruieren und ein detaillierteres Bild auf die Mensch-Umwelt-Beziehung mit Schwerpunkt zu bekommen

Characterization and Comparative Study of Energy Efficient Mechanochemically Induced NASICON Sodium Solid Electrolyte Synthesis

In recent years, there is growing interest in solid-state electrolytes due to their many promising properties, making them key to the future of battery technology. This future depends among other things on easy processing technologies for the solid electrolyte. The sodium superionic conductor (NASICON) Na3Zr2Si2PO12 is a promising sodium solid electrolyte; however, reported methods of synthesis are time consuming. To this effect, attempt was made to develop a simple time efficient alternative processing route. Firstly, a comparative study between a new method and commonly reported methods was carried out to gain a clear insight into the mechanism of formation of sodium superionic conductors (NASICON). It was observed that through a careful selection of precursors, and the use of high-energy milling (HEM) the NASICON conversion process was enhanced and optimized, this reduces the processing time and required energy, opening up a new alternative route for synthesis. The obtained solid electrolyte was stable during Na cycling vs. Na-metal at 1 mA cm−1, and a room temperature conductivity of 1.8 mS cm−1 was attained

Stolniceni – Excavation results from the 2017 campaign

During extensive field work, different aspects of the large Tripolye settlement Stolniceni I in northwest Moldova were investigated by an international team. These investigations allow a reassessment of these population agglomerations from the first half of the 4th millennium BCE in Moldova and their comparison with more eastern Tripolye sites. The investigations carried out in 2017 included the completion of the archaeo-magnetic survey of the settlement and the targeted archaeological excavations of various types of contexts. Besides the investigation of a ditch enclosing the site and test trenches in different parts of the settlement, the excavations focused mainly on two areas in the north of the settlement: On the one hand, excavations were carried out in a pottery production complex, including the uncovering of a pottery kiln, several pits and a dwelling. On the other hand, one specific ‘ash-mound-like’ anomaly was investigated which are arranged in regular intervals at the periphery of the settlement. The archaeological excavations were accompanied by comprehensive archaeobotanical analyses, which provide an insight into the subsistence and wood selection in the settlement, as well as permitting environmental reconstructions. A larger series of 14C-datings proves the occupation of the settlement Stolniceni I over a longer period between about 3925 and 3700 BCE

Modelling landscape transformation at the Chalcolithic Tripolye mega-site of Maidanetske (Ukraine): Wood demand and availability

Wood was a crucial resource for prehistoric societies, for instance, as timber for house construction and as fuel. In the case of the exceptionally large Chalcolithic Tripolye ‘mega-sites’ in central Ukraine, thousands of burnt buildings, indicating huge population agglomerations, hint at such a massive use of wood that it raises questions about the carrying capacity of the sensitive forest-steppe environment. In this contribution, we investigate the wood demand for the mega-site of Maidanetske (3990–3640 BCE), as reconstructed based on wood charcoal data, wood imprints on daub and the archaeomagnetometry-based settlement plan. We developed a regional-scale model with a fuzzy approach and applied it in order to simulate the potential distribution and extent of woodlands before and after Chalcolithic occupation. The model is based upon the reconstructed ancient land surface, soil information derived from cores and the potential natural woodland cover reconstructed based on the requirements of the prevailing ancient tree species. Landscape scenarios derived from the model are contrasted and cross-checked with the archaeological empirical data. We aim to understand whether the demand for wood triggered the site development. Did deforestation and consequent soil degradation and lack of resources initiate the site’s abandonment? Or, alternatively, did the inhabitants develop sustainable woodland management strategies? Starting from the case study of Maidanetske, this study provides estimates of the extent of human impact on both carrying capacity and landscape transformations in the sensitive transitional foreststeppe environment. Overall, the results indicate that the inhabitants of the Chalcolithic site did not suffer from a significant shortage in the wood resource at any time of inhabitation in the contexts of the different scenarios provided by the model. An exception is given by the phase of maximum house construction and population within a scenario of dry climatic conditions

[b]-Annulated Halogen-Substituted Indoles as Potential DYRK1A Inhibitors

Since hyperactivity of the protein kinase DYRK1A is linked to several neurodegenerative disorders, DYRK1A inhibitors have been suggested as potential therapeutics for Down syndrome and Alzheimer's disease. Most published inhibitors to date suffer from low selectivity against related kinases or from unfavorable physicochemical properties. In order to identify DYRK1A inhibitors with improved properties, a series of new chemicals based on [b]-annulated halogenated indoles were designed, synthesized, and evaluated for biological activity. Analysis of crystal structures revealed a typical type-I binding mode of the new inhibitor 4-chlorocyclohepta[b]indol-10(5H)-one in DYRK1A, exploiting mainly shape complementarity for tight binding. Conversion of the DYRK1A inhibitor 8-chloro-1,2,3,9-tetrahydro-4H-carbazol-4-one into a corresponding Mannich base hydrochloride improved the aqueous solubility but abrogated kinase inhibitory activity

Experimental investigation of MHD impact on argon plasma flows by variation of magnetic flux density

The interaction between a probe body and argon plasma flow is investigated to examine to what extent the probe head temperature and the bow shock distance can be influenced by applying a strong magnetic field. The experiments are performed using a strong permanent magnet installed inside a probe body with a spherical, coated probe head. Former investigations showed strong influence on the bow shock geometry but also on the inflow plasma jet. Several boundary conditions have been varied to evaluate their influence toward the experiment. For an uncoated probe head the measured MHD impact was found to be of the same order of magnitude as for the coated case. Electrical isolation of the probe toward the vacuum chamber yielded only slight influence. The variation of the field strength was realized by changing the amount of magnet segments installed. Pictures were analyzed to minute the MHD interaction for each test case. It was found that the bow shock distance increased and the temperature of the probe head decreased while increasing the magnetic field density. This analysis precedes a thorough characterization of the plasma condition

Introgressiomics: a new approach for using crop wild relatives in breeding for adaptation to climate change

[EN] The need to boost agricultural production in the coming decades in a climate change scenario requires new approaches for the development of new crop varieties that are more resilient and more efficient in the use of resources. Crop wild relatives (CWRs) are a source of variation for many traits of interest in breeding, in particular tolerance to abiotic and biotic stresses. However, their potential in plant breeding has largely remained unexploited. CWRs can make an effective contribution to broadening the genetic base of crops and to introgressing traits of interest, but their direct use by breeders in breeding programs is usually not feasible due to the presence of undesirable traits in CWRs (linkage drag) and frequent breeding barriers with the crop. Here we call for a new approach, which we tentatively call 'introgressiomics', which consists of mass scale development of plant materials and populations with introgressions from CWRs into the genetic background of crops. Introgressiomics is a form of pre-emptive breeding and can be focused, when looking for specific phenotypes, or un-focused, when it is aimed at creating highly diverse intro-gressed populations. Exploring germplasm collections and identifying adequate species and accessions from different genepools encompassing a high diversity, using different strategies like the creation of germplasm diversity sets, Focused identification of germplasm strategy (FIGS) or gap analysis, is a first step in introgressiomics. Interspecific hybridization and backcrossing is often a major barrier for introgressiomics, but a number of techniques can be used to potentially overcome these and produce introgression populations. The generation of chromosome substitution lines (CSLs), introgression lines (ILs), or multiparent advanced inter-cross (MAGIC) populations by means of marker-assisted selection allows not only the genetic analysis of traits present in CWRs, but also developing genetically characterized elite materials that can be easily incorporated in breeding programs. Genomic tools, in particular high-throughput molecular markers, facilitate the characterization and development of introgressiomics populations, while new plant breeding techniques (NPBTs) can enhance the introgression and use of genes from CWRs in the genetic background of crops. An efficient use of introgressiomics populations requires moving the materials into breeding pipelines. In this respect public-private partnerships (PPPs) can contribute to an increased use of introgressed materials by breeders. We hope that the introgressiomics approach will contribute to the development of a new generation of cultivars with dramatically improved yield and performance that may allow coping with the environmental changes caused by climate change while at the same time contributing to a more efficient and sustainable agriculture.This work was undertaken as part of the initiative "Adapting Agriculture to Climate Change: Collecting, Protecting and Preparing Crop Wild Relatives", which is supported by the Government of Norway. The Project is managed by the Global Crop Diversity Trust with the Millennium Seed Bank of the Royal Botanic Gardens, Kew and implemented in partnership with national and international gene banks and plant breeding institutes around the world. For further information see the project website: http://www.cwrdiversity.org/. This work has also been funded in part by European Union's Horizon 2020 research and innovation programme under Grant agreement No 677379 (G2P-SOL) and from Spanish Ministerio de Economia y Competitividad and Fondo Europeo de Desarrollo Regional (Grant AGL2015-64755-R from MINECO/FEDER, EU). Pietro Gramazio is grateful to Universitat Politecnica de Valencia for a pre-doctoral (Programa FPI de la UPV-Subprograma 1/2013 call) contract.Prohens Tomás, J.; Gramazio, P.; Plazas Ávila, MDLO.; Dempewolf, H.; Kilian, B.; Díez-Niclós, MJTDJ.; Fita, A.... (2017). Introgressiomics: a new approach for using crop wild relatives in breeding for adaptation to climate change. 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ACE2 is the critical in vivo receptor for SARS-CoV-2 in a novel COVID-19 mouse model with TNF-and IFN?-driven immunopathology

Despite tremendous progress in the understanding of COVID-19, mechanistic insight into immunological, disease-driving factors remains limited. We generated maVie16, a mouse-adapted SARS-CoV-2, by serial passaging of a human isolate. In silico modeling revealed how only three Spike mutations of maVie16 enhanced interaction with murine ACE2. maVie16 induced profound pathology in BALB/c and C57BL/6 mice, and the resulting mouse COVID-19 (mCOVID-19) replicated critical aspects of human disease, including early lymphopenia, pulmonary immune cell infiltration, pneumonia, and specific adaptive immunity. Inhibition of the proinflammatory cyto-kines IFN? and TNF substantially reduced immunopathology. Importantly, genetic ACE2-deficiency completely prevented mCOVID-19 development. Finally, inhalation therapy with recombinant ACE2 fully protected mice from mCOVID-19, revealing a novel and efficient treatment. Thus, we here present maVie16 as a new tool to model COVID-19 for the discovery of new therapies and show that disease severity is determined by cytokine-driven immunopathology and critically dependent on ACE2 in vivo. © Gawish et al