Enquête sur les étudiants fréquentant la bibliothèque Mériadeck de Bordeaux (2007)

Résultats de l\u27enquête de la bibliothèque Mériadeck de Bordeaux menée auprès des étudiants en 2007. Questionnaire, résultats détaillés, analyse et tableaux Excel

Enquête sur l\u27élargissement des horaires d\u27ouverture de Mériadeck

Enquête pour recueillir les propositions du public (de la centrale et des quartiers) concernant l’élargissement des horaires d’ouverture de la bibliothèque Mériadeck à Bordeaux (1-10 février 2007). Le document reprend les modalités de l\u27enquête et les données chiffrées

Phylogenetic and behavioral differentiation in the canyon treefrog, Hyla arenicolor

Title from PDF of title page (University of Missouri--Columbia, viewed on May 29, 2012).The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.Dissertation advisor: Dr. H. Carl GerhardtVita.Ph. D. University of Missouri-Columbia 2011."December 2011"My research interests lie in understanding the process of speciation. In my dissertation I set out to do this by examining the first step in potential speciation, population differentiation. In my dissertation research I examined both genetic and behavioral differentiation within the wide-ranging canyon treefrog, Hyla arenicolor. I documented variation in male advertisement calls throughout the range, assessed the role of female preference in promoting behavioral reproductive isolation, and inferred intraspecific phylogenies using both sequence data and AFLP markers. I describe biologically significant differences in call properties among the geographically distant Mexican lineages relative to differences found among the USA populations. Results from female playback tests show that differences in pulse rate, call rate and call duration observed among the Mexican lineages were large enough to cause females from USA populations to discriminate against these calls, suggesting a role for behavioral reproductive isolation in the continued divergence of these lineages. Using molecular phylogenetic techniques, I found evidence for past hybridization and mtDNA introgression between two H. arenicolor populations and the sister species, H. eximia. Overall, my results provide insight into the complex evolutionary history of this group, have implications for the study of character evolution in this group, and emphasize the need for phylogeographic studies to expand sampling to include closely related, syntopic species.Includes bibliographical reference

Metabarcoding assays for the detection of freshwater mussels (Unionida) with environmental DNA

Freshwater mussels of the order Unionida are a widely distributed taxon that are important in maintaining freshwater ecosystems and are also highly imperiled throughout the world. Monitoring of mussel populations with environmental DNA (eDNA) is an attractive alternative to traditional methods because it is noninvasive and requires less labor and taxonomic knowledge from field personnel. We developed eDNA metabarcoding assays specific to freshwater mussels and tested them at six sites in the Clinch River, located in the southeastern United States. Our objective was to determine the utility of eDNA metabarcoding for future monitoring of mussel populations and restoration efforts in this watershed. Two metabarcoding assays that target the mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes were developed and tested. Our assays appear to be order specific, amplifying members from the two families found in North America, Unionidae and Margaritiferidae, while not amplifying nontarget fish or other bivalve species. From the field collected samples, our assays together detected 19 species, eight of which are listed as federally endangered. The assays also detected 42%, 58%, and 54% of the species identified by recent quantitative visual mussel surveys at three sampling sites. Increased sampling effort by processing a greater water volume or number of samples will likely increase species detections. These eDNA metabarcoding assays may enable enhanced monitoring of freshwater mussel assemblages and subsequently inform conservation efforts

THE IMPACT OF PHYSICAL AND SPORTS REHABILITATION OF PEOPLE WITH CEREBRAL PALSY ON THE PROPRIOCEPTIVE SYSTEM IMPROVEMENT

Introduction: Cerebral palsy occurs as a result of affecting those parts of the central nervous system that control the muscles and are responsible for the balance and movements arbitrariness. It has a number of forms with varying degrees of damage to the central nervous system and impaired physiological functions. And this certainly affects the perception. Due to perception distortion, sensory interpretation is slowed down and incorrectly analyzed in the cerebral cortex. The improvement of the motor sphere implies the development of general and small motor skills, and the improvement of movements coordination. The correction of motor sphere disorders should be done in a comprehensive, systematic manner, with the involvement of specialists (neurologists, physical therapists, rehabilitologists, sensory therapists). This will help to determine the content of motor sphere correction exercises and define the step-by-step measures for physical condition improvement. Aim: to investigate the impact of physical and sports rehabilitation measures of people with cerebral palsy disabilities on improving the proprioceptive system basing on an analysis of the practical application of the bocce game program. Methodology: SIPT (Sensory Integration and Praxis Tests) breach detection study. Bocce game lessons were tested (as a method of physical and sport rehabilitation) in order to improve the sensory system. Object of the study – 10 people aged 28–35 with a clinical diagnosis of "Cerebral palsy" with impaired mobility, who are using a trolley and are undergoing rehabilitation at the rehabilitation center in Lviv. Results: It was found that 9 out of 10 participants had dysarthric disorders. General somatic attenuation and slow development of locomotor functions are accompanied by a lag in the motor sphere development of the people with cerebral palsy. Conclusion: The study found that engaging in sports and exercise leads to increased physical health and improved gross fine motor skills related to the sensorimotor syste

In vitro evaluation of the presence of apical deviation with employment of automated handpieces with continuous and alternate motion for root canal preparation

O presente trabalho avaliou, in vitro, a presença de desvio apical no preparo de canais radiculares em raízes mésio-vestibulares de molares superiores humanos utilizando-se de peças automatizadas de giro contínuo, sistema Pow R (Moyco Union Broach) e giro alternado, sistema M4 (Kerr - USA). A análise da presença de desvios apicais foi realizada por meio de radiografias obtidas na plataforma radiográfica confeccionada para o experimento. Os resultados mostraram não haver diferença estatisticamente significante quanto à avaliação da presença de desvio entre os dois sistemas. Não houve correlação entre o grau de curvatura das raízes e o grau de desvio apical, no sistema de giro contínuo. No entanto, o sistema de giro alternado apresentou diferença estatística de correlação direta e grau médio.The present study conducted an in vitro evaluation of the presence of apical deviation on the root canal preparation of the mesiobuccal roots of human maxillary molars employing automated handpieces with continuous motion, the Pow R system (Moyco Union Broach), and alternate motion, the M4 system (Kerr). Analysis of the presence of apical deviation was carried out by means of radiographs obtained on a radiographic desk fabricated for the study. The results demonstrated the lack of statistically significant differences between the two systems as to the evaluation of the presence of apical deviation. There was no correlation between the degree of curvature of the roots and the degree of apical deviation for the continuous motion system. However, there was a statistic difference for the alternate motion system, with a direct correlation of moderate degree

Recent advances in environmental DNA-based biodiversity assessment and conservation

Special issue Environmental DNA‐based biodiversity assessment and conservation management in the Anthropocene.-- 4 pagesKnowledge of species distribution across space and time is critical to ecological conservation and environmental management at the local, regional and global scales (Albert et al., 2021). Traditional morphology-based surveys on either single-celled protists or larger animals and plants are time-consuming and largely expert-dependent (Baird & Hajibabaei, 2012; Liu et al., 2017; Yang et al., 2017). Recently, there has been considerable interest in the detection of environmental DNA (eDNA) fragments to allow species identification and monitoring within different environments, including soil, sediment, water, snow or air (Abdullah et al., 2021; Rees et al., 2014; Xie et al., 2018). The eDNA analysis can be used to detect common, endangered, invasive or rare species (Liu et al., 2019; Sepulveda et al., 2020), and provide a potent tool for elucidating mechanistic insights into ecological and evolutionary processes (Baird & Hajibabaei, 2012; Bohmann et al., 2014; Pawlowski et al., 2021). In past decades, eDNA metabarcoding has been increasingly used to study the present and past biodiversity from population to community levels, and eDNA-based surveys have revolutionized studies in ecology and biodiversity sciences, particularly in aquatic ecosystems (Euclide et al., 2021; Valentini et al., 2016). The significance of various human activities has resulted in multiple interacting environmental stressors in all types of ecosystems (Pukk et al., 2021; Yang et al., 2022). Such stressors, including global climate change, invasive species, chemical pollution and habitat loss, have led to biodiversity crises and threatened the human sustainability and ecosystem health (Osathanunkul & Minamoto, 2021; Yang et al., 2017). Comprehensive biodiversity assessment and conservation management are prerequisites for addressing these significant challenges in the Anthropocene (Mace et al., 2012; Sepulveda et al., 2020). Indeed, effective biodiversity assessment and conservation management require a deep understanding of organisms’ geographical distributions and their respective roles in ecosystem processes and services (Mo et al., 2021; West et al., 2021). However, researchers and conservation managers have encountered numerous obstacles in answering these fundamental and applied research questions at the local, regional and global scales. The aim of this special issue—Environmental DNA-based biodiversity assessment and conservation—was to provide a selection of studies that highlight the utility and diversity of eDNA-based research for biodiversity assessment and conservation management within marine and freshwater ecosystems. This special issue includes 12 articles that advance our knowledge of eDNA. Together, these studies deliver compelling evidence for successful applications of eDNA-based surveys in aquatic ecosystems in the AnthropoceneThis work was supported by the National Natural Science Foundation of China (91851104) and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA23040302)With the institutional support of the ‘Severo OchoaCentre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewe

Enquête auprès du public de la bibliothèque municipale de Bordeaux (2002)

Résultats de l\u27enquête de la Bibliothèque municipale de Bordeaux menée auprès du public en 2002. Questionnaire, résultats détaillés et analyse

Using eDNA to detect the distribution and density of invasive crayfish in the Honghe-Hani rice terrace World Heritage site

The Honghe-Hani landscape in China is a UNESCO World Natural Heritage site due to the beauty of its thousands of rice terraces, but these structures are in danger from the invasive crayfish Procambarus clarkii. Crayfish dig nest holes, which collapse terrace walls and destroy rice production. Under the current control strategy, farmers self-report crayfish and are issued pesticide, but this strategy is not expected to eradicate the crayfish nor to prevent their spread since farmers are not able to detect small numbers of crayfish. Thus, we tested whether environmental DNA (eDNA) from paddy-water samples could provide a sensitive detection method. In an aquarium experiment, Real-time Quantitative polymerase chain reaction (qPCR) successfully detected crayfish, even at a simulated density of one crayfish per average-sized paddy (with one false negative). In a field test, we tested eDNA and bottle traps against direct counts of crayfish. eDNA successfully detected crayfish in all 25 paddies where crayfish were observed and in none of the 7 paddies where crayfish were absent. Bottle-trapping was successful in only 68% of the crayfish-present paddies. eDNA concentrations also correlated positively with crayfish counts. In sum, these results suggest that single samples of eDNA are able to detect small crayfish populations, but not perfectly. Thus, we conclude that a program of repeated eDNA sampling is now feasible and likely reliable for measuring crayfish geographic range and for detecting new invasion fronts in the Honghe Hani landscape, which would inform regional control efforts and help to prevent the further spread of this invasive crayfish