Temperature modulates compensatory responses to food limitation at metamorphosis in a marine invertebrate

Under climate change, increased temperatures combined with food limitation may be critical for species with complex life cycles, if high growth rates characterise the larval development. We studied the effect of increased temperature and food limitation on larval survival and on functional traits (developmental time, body mass, elemental composition, growth) at moulting and metamorphosis in the crab Carcinus maenas collected in the North Sea (Helgoland, Germany). We followed the approach of models of metamorphosis integrating responses of body mass and developmental time to increased temperature and food limitation. We also evaluated if body mass decreased with temperature (according to the temperature-size rule) and if developmental time followed an inverse exponential reduction (expected from some metabolic theories), as both trends are relevant for modelling effects of climate change on fitness and population connectivity. Larvae produced by four females during the reproductive period (i.e. spring-summer 2016) were reared separately from hatching to metamorphosis to the megalopa at two food conditions (ad libitum and low food availability), and at four temperatures covering the range experienced in the field (20°C). Survival and developmental rates were obtained by daily monitoring of the experiments. Biomass data (body mass and elemental composition) were obtained by sampling larvae at the zoea IV and megalopa stages and further processed with standard methods (see Torres & Giménez 2020 for details). We propose that integrative studies of traits at metamorphosis could be a basis to develop a mechanistic understanding of how species with complex life cycles will respond to climate change. Such models could eventually include hormonal and metabolic regulation of development as drivers of responses to environmental change

Stellar Kinematics of the Andromeda II Dwarf Spheroidal Galaxy

We present kinematical profiles and metallicity for the M31 dwarf spheroidal (dSph) satellite galaxy Andromeda II (And II) based on Keck DEIMOS spectroscopy of 531 red giant branch stars. Our kinematical sample is among the largest for any M31 satellite and extends out to two effective radii (r_eff = 5.3' = 1.1 kpc). We find a mean systemic velocity of -192.4+-0.5 km/s and an average velocity dispersion of sigma_v = 7.8+-1.1 km/s. While the rotation velocity along the major axis of And II is nearly zero (<1 km/s), the rotation along the minor axis is significant with a maximum rotational velocity of v_max=8.6+-1.8 km/s. We find a kinematical major axis, with a maximum rotational velocity of v_max=10.9+-2.4 km/s, misaligned by 67 degrees to the isophotal major axis. And II is thus the first dwarf galaxy with evidence for nearly prolate rotation with a v_max/sigma_v = 1.1, although given its ellipticity of epsilon = 0.10, this object may be triaxial. We measured metallicities for a subsample of our data, finding a mean metallicity of [Fe/H] = -1.39+- 0.03 dex and an internal metallicity dispersion of 0.72+-0.03 dex. We find a radial metallicity gradient with metal-rich stars more centrally concentrated, but do not observe a significant difference in the dynamics of two metallicity populations. And II is the only known dwarf galaxy to show minor axis rotation making it a unique system whose existence offers important clues on the processes responsible for the formation of dSphs.Comment: 14 pages, 10 figures, 4 tables, accepted for publication in Ap

The Nature and Origin of Substructure in the Outskirts of M31. I. Surveying the Stellar Content with HST/ACS

We present the largest and most detailed survey to date of the stellar populations in the outskirts of M31 based on the analysis of 14 deep HST/ACS pointings spanning the range 11.5-45.0 kpc. We conduct a quantitative comparison of the resolved stellar populations in these fields and identify several striking trends. The color-magnitude diagrams (CMDs), which reach ~3 magnitudes below the red clump, can be classified into two main categories based on their morphologies. `Stream-like' fields, so named for their similarity to the CMD of the giant stellar stream, are characterized by a red clump that slants bluewards at fainter magnitudes and an extended horizontal branch. On the other hand, `disk-like' fields exhibit rounder red clumps with significant luminosity width, lack an obvious horizontal branch and show evidence for recent star formation (~0.25 - 2.0 Gyr ago). We compare the spatial and line-of-sight distribution of stream-like fields with a recent simulation of the giant stream progenitor orbit and find an excellent agreement. These fields, found across the face of M31, attest to the high degree of pollution caused by this event. Disk-like material resides in the extended disk structure of M31 and is detected out to 44 kpc (projected); the uniform populations in these fields, including the ubiquitous presence of young populations, and the strong rotation reported elsewhere are most consistent with a scenario in which this structure has formed through heating and disruption of the existing thin disk, perhaps due to the impact of the giant stream progenitor. Our comparative analysis sheds new light on the likely composition of two of the ultra-deep pointings formerly presented as pure outer disk and pure halo in the literature.Comment: Accepted to AJ. ftp://ftp.roe.ac.uk/pub/jcr/richardson2008.pdf contains high resolution figures. 16 pages, 10 figures; best seen in color on above lin

Racial Disparity in Police Stop and Searches in England and Wales

Data published by the United Kingdom's Ministry for Justice clearly shows that, compared to persons who were White, members of racial minorities in England, particularly Blacks, were far more likely to be stopped and searched by the police. The question is whether such racial disparity in stops and searches could be justified by racial disparities in offending? Or whether the disparity in stop and searches exceeded the disparity in offending? This paper proposes a method for measuring the amount of excess in racial disparity in police stop and searches. Using the most recently published Ministry of Justice data (for 2007/08) for Police Areas in England and Wales it concludes that while in several Areas there was no excess to racial disparity in police stop and searches, there was, on the basis of the methodology proposed in the paper, evidence of such excess in some Police Areas of England and Wales

The Star Formation History and Dust Content in the Far Outer Disc of M31

We present a detailed analysis of two fields located 26 kpc (~5 scalelengths) from the centre of M31. One field samples the major axis populations--the Outer Disc field--while the other is offset by ~18' and samples the Warp in the stellar disc. The CMDs based on HST/ACS imaging reach old main-sequence turn-offs (~12.5 Gyr). We apply the CMD-fitting technique to the Warp field to reconstruct the star formation history (SFH). We find that after undergoing roughly constant SF until about 4.5 Gyr ago, there was a rapid decline in activity and then a ~1.5 Gyr lull, followed by a strong burst lasting 1.5 Gyr and responsible for 25% of the total stellar mass in this field. This burst appears to be accompanied by a decline in metallicity which could be a signature of the inflow of metal-poor gas. The onset of the burst (~3 Gyr ago) corresponds to the last close passage of M31 and M33 as predicted by detailed N-body modelling, and may have been triggered by this event. We reprocess the deep M33 outer disc field data of Barker et al. (2011) in order to compare consistently-derived SFHs. This reveals a similar duration burst that is exactly coeval with that seen in the M31 Warp field, lending further support to the interaction hypothesis. The complex SFHs and the smoothly-varying age-metallicity relations suggest that the stellar populations observed in the far outer discs of both galaxies have largely formed in situ rather than migrated from smaller galactocentric radii. The strong differential reddening affecting the CMD of the Outer Disc field prevents derivation of the SFH. Instead, we quantify this reddening and find that the fine-scale distribution of dust precisely follows that of the HI gas. This indicates that the outer HI disc of M31 contains a substantial amount of dust and therefore suggests significant metal enrichment in these parts, consistent with inferences from our CMD analysis.Comment: Abstract shortened. 17 pages, 12 figures (+ 6 pages & 5 figures in Appendix). MNRAS, in pres

Circadian Cycles of Gene Expression in the Coral, Acropora millepora

Background: Circadian rhythms regulate many physiological, behavioral and reproductive processes. These rhythms are often controlled by light, and daily cycles of solar illumination entrain many clock regulated processes. In scleractinian corals a number of different processes and behaviors are associated with specific periods of solar illumination or nonillumination—for example, skeletal deposition, feeding and both brooding and broadcast spawning. Methodology/Principal Findings: We have undertaken an analysis of diurnal expression of the whole transcriptome and more focused studies on a number of candidate circadian genes in the coral Acropora millepora using deep RNA sequencing and quantitative PCR. Many examples of diurnal cycles of RNA abundance were identified, some of which are light responsive and damped quickly under constant darkness, for example, cryptochrome 1 and timeless, but others that continue to cycle in a robust manner when kept in constant darkness, for example, clock, cryptochrome 2, cycle and eyes absent, indicating that their transcription is regulated by an endogenous clock entrained to the light-dark cycle. Many other biological processes that varied between day and night were also identified by a clustering analysis of gene ontology annotations. Conclusions/Significance: Corals exhibit diurnal patterns of gene expression that may participate in the regulation of circadian biological processes. Rhythmic cycles of gene expression occur under constant darkness in both populations o

What is metamorphosis?

Metamorphosis (Gr. meta- “change” + morphe “form”) as a biological process is generally attributed to a subset of animals: most famously insects and amphibians, but some fish and many marine invertebrates as well. We held a symposium at the 2006 Society for Integrative and Comparative Biology (SICB) annual meeting in Orlando, FL (USA) to discuss metamorphosis in a comparative context. Specifically, we considered the possibility that the term “metamorphosis” could be rightly applied to non-animals as well, including fungi, flowering plants, and some marine algae. Clearly, the answer depends upon how metamorphosis is defined. As we participants differed (sometimes quite substantially) in how we defined the term, we decided to present each of our conceptions of metamorphosis in 1 place, rather than attempting to agree on a single consensus definition. Herein we have gathered together our various definitions of metamorphosis, and offer an analysis that highlights some of the main similarities and differences among them. We present this article not only as an introduction to this symposium volume, but also as a reference tool that can be used by others interested in metamorphosis. Ultimately, we hope that this article—and the volume as a whole—will represent a springboard for further investigations into the surprisingly deep mechanistic similarities among independently evolved life cycle transitions across kingdoms

Protein Evolution by Molecular Tinkering: Diversification of the Nuclear Receptor Superfamily from a Ligand-Dependent Ancestor

Phylogenetic reconstruction of the structure and function of the ancestor of the nuclear receptor protein family reveals how functional diversity evolves by subtle tinkering with an ancestral template

A deep kinematic survey of planetary nebulae in the Andromeda Galaxy using the Planetary Nebula Spectrograph

We present a catalogue of positions, magnitudes and velocities for 3300 emission-line objects found by the Planetary Nebula Spectrograph in a survey of the Andromeda Galaxy, M31. Of these objects, 2615 are found likely to be planetary nebulae (PNe) associated with M31. Initial results from this survey include: the likely non-existence of Andromeda VIII; a universal PN luminosity function, with the exception of a small amount of obscuration, and a small offset in normalization between bulge and disk components; very faint kinematically-selected photometry implying no cut-off in the disk to beyond 4 scalelengths and no halo population in excess of the bulge out to 10 effective bulge radii; disk kinematics that show significant dispersion and asymmetric drift out to large radii, consistent with a warm flaring disk; and no sign of any variation in kinematics with PN luminosity, suggesting that PNe arise from a fairly uniform population of old stars.Comment: Accepted by MNRAS. 23 pages, 37 figures. A full resolution version is available at http://www.astro.rug.nl/~pns/pns_pub.htm