5 research outputs found
Detection of sexually transmitted infection and human papillomavirus in negative cytology by multiplex-PCR
<p>Abstract</p> <p>Background</p> <p>The aim of this study was to determine the prevalence of human papillomavirus (HPV) and 15 species that cause sexually transmitted infections (STIs) in negative cytology. In addition, we compared the diagnostic performance of multiplex polymerase chain reaction (PCR) with widely available techniques used to detect HPV.</p> <p>Methods</p> <p>We recruited 235 women of reproductive age who had negative cytology findings in a liquid-based cervical smear. STIs were identified by multiplex PCR, and HPV genotypes by multiplex PCR, hybrid capture 2, and DNA microaray; discordant results were analyzed by direct sequencing.</p> <p>Results</p> <p>Approximately 96.6% of patients with negative cytology results were positive for pathogens that cause STIs. The pathogens most frequently detected were <it>Gardnerella vaginalis, Ureaplasma urealyticum</it>. The incidence of HPV in negative cytology was 23.3%. Low-risk HPV infection was significantly correlated with <it>Chalmaydia trachomatis</it>, and high-risk HPV infection was significantly correlated with <it>Group ÎČ streptococcus</it>. The analytical sensitivities of the multiplex PCR and DNA microarray were higher than 80%, and the analytical specificity was nearly 100% for all tests.</p> <p>Conclusions</p> <p>Multiplex PCR yielded results that most of patients with negative cytology were positive for pathogens that cause STIs, and were more similar to that of DNA microarray, than that of hybrid capture 2 in terms of analytical sensitivity and prediction value of HPV infection.</p
Ăvaluation des compĂ©tences pour la pratique de la biomĂ©trie Ă©chographique fĆtale : validation prospective du score OSAUS METHOD
International audienceObjectivesTo develop and validate a customized variant for fetal biometry of the generic OSAUS score (Objective Structured Assessment of Ultrasound Skills)MethodsThe 5-points OSAUS METHOD grid was elaborated by defining five target skills specific to fetal biometry for each thematic item of the generic score. The level of skills of 43 trainees was prospectively assessed during an ultrasound examination by using this grid. The results of the ânoviceâ level group (experience < 10 ultrasounds) were compared to those of the âintermediateâ level group (experience â„ 10 ultrasounds) (I). The reached/non-reached skills ratio within the different items composing the score allowed the identification of priority areas of improvement (II). Previously published distribution and actual distribution of ratings according to the generic pass/fail score were compared (III).ResultsMedian scores of ânovicesâ (n = 29) and âintermediatesâ (n = 14) groups were statistically different, 1.87 (±0.75) and 3.31 (±0.83) (P = 1.85-5), respectively and corresponded to the pre-existing experience (I). A lower ratio of reached skill allowed the identification of âdocumentation of the examinationâ item as a priority area of improvement for both groups (II). The relevance of the pass/fail score is consolidated, even if an overlap was observed between novices and intermediates groups (III).ConclusionsThe relevancy and feasibility of using OSAUS scoring method for fetal biometry are supported. In addition, the possibility of comparisons with generic OSAUS remains.ObjectifsDĂ©velopper et Ă©valuer la pertinence clinique dâune version du score gĂ©nĂ©rique OSAUS (Objective Structured Assessment of Ultrasound Skills) dĂ©diĂ©e Ă la biomĂ©trie fĆtale.MatĂ©riel et mĂ©thodesCinq compĂ©tences cibles spĂ©cifiques Ă la biomĂ©trie fĆtale ont Ă©tĂ© dĂ©finies pour chaque item thĂ©matique du score gĂ©nĂ©rique permettant dâĂ©tablir une grille dâĂ©valuation OSAUS-METHOD sur 5 points (MEasurements TeacHing in Obstetrics Design). Le niveau de compĂ©tence de 43 Ă©tudiants a Ă©tĂ© Ă©valuĂ© Ă lâaide grille OSAUS-METHOD (MEasurements TeacHing in Obstetrics Design) lors dâexamens pratiques. Les notes des Ă©tudiants dĂ©butants (expĂ©rience pratique < 10 examens) ont Ă©tĂ© comparĂ©es Ă celles des Ă©tudiants de niveau intermĂ©diaire (expĂ©rience pratique â„ 10 examens) (I). La proportion de compĂ©tences validĂ©es ou non au sein de chacun des items thĂ©matiques du score a permis dâidentifier les axes de progression prioritaires (II). La distribution des notes obtenues par rapport au seuil de certification utilisĂ© pour le score gĂ©nĂ©rique a Ă©tĂ© comparĂ©e Ă celle prĂ©cĂ©demment publiĂ©e (III).RĂ©sultatsLe score moyen au sein du groupe « dĂ©butants » (n = 29) Ă©tait significativement plus faible que celui au sein du « intermĂ©diaires » (n = 14) (1,87 ± 0,75 et 3,31 ± 0,83 respectivement, p = 1,855) (I). Une proportion plus importante de compĂ©tences non validĂ©e a permis dâidentifier lâitem « documentation de lâexamen » comme axe de progression prioritaire (II). La pertinence du score seuil pour discriminer les Ă©tudiants en fonction du niveau dâexpertise est confortĂ©e mais un minime chevauchement entre les groupes de niveau est observĂ© (III).ConclusionLa faisabilitĂ© et la pertinence de lâutilisation dâun score OSAUS dĂ©diĂ© Ă la biomĂ©trie sont confirmĂ©es ainsi que la possibilitĂ© de comparaisons avec le score OSAUS gĂ©nĂ©rique