Valoración de un diagnóstico "in vitro" de IGG/IGE en perros sospechosos de padecer alergia/Intolerancia alimentaria

La alergia/intolerancia alimentaria es una patología frecuentemente relacionada con la dermatitis atópica canina. Aunque las dietas de eliminación son el "golden standard" para seleccionar los alimentos que pueden consumir los animales afectados, este método es largo y complicado, y en muchas ocasiones resulta difícil contar con la colaboración del propietario

Eficacia de la hiposensibilización en perros con dermatitis atópica

Valorar la respuesta a la hiposensibilización de un grupo de perros con dermatitis atópica a partir de los resultados obtenidos con el test serológico E.L.I.S.A

Eficacia de la hiposensibilización en perros con dermatitis atópica

Valorar la respuesta a la hiposensibilización de un grupo de perros con dermatitis atópica a partir de los resultados obtenidos con el test serológico E.L.I.S.A

Comparison of artificial neural network models and empirical and semi-empirical equations for daily reference evapotranspiration estimation in the Basque Country (Northern Spain)

Reference evapotranspiration (ETo) determination is a key factor for water balance and irrigation scheduling. Evapotranspiration can be measured directly by high-cost micrometeorological techniques, or estimated by mathematical models. The combination equation of Penman-Monteith, modified by Allen et al. [Allen, R.G., Pereira, L.S., Raes, D., Smith, M., 1998. Crop evapotranspiration. Guidelines for computing crop water requirements. FAO Irrigation and Drainage, Paper no. 56. FAO, Rome] (PM56), is the reference equation for ETo estimation. This method is also appropriate for the calibration of other ETo estimation equations. The utilization of these calibrated ETo equations is recommended in the absence of data of any of the meteorological parameters necessary for the application of PM56. In addition to the use of classic ETo equations, the adoption of artificial neural network (ANN) models for the estimation of daily ETo has been evaluated in this study. ANNs are mathematical models, whose architecture has been inspired by biological neural networks. They are highly appropriate for the modelling of non-linear processes, which is the case of the evapotranspiration process. Seven ANNs (with different input combinations) have been implemented and compared with ten locally calibrated empirical and semi-empirical ETo equations and variants of these equations (with estimated meteorological parameters as inputs). The comparisons have been based on statistical error techniques, using PM56 daily ETo values as a reference. ANNs have obtained better results than the locally calibrated ETo equations in the three groups of evaluated methods: temperature and/or relative humidity-based methods (0.385 mm d-1 of root mean square error (RMSE)), solar radiation-based methods (0.238 mm d-1 of RMSE), and methods based on similar requirements to those of PM56 except for the estimation of solar radiation and/or relative humidity (0.285 mm d-1 of RMSE).

Strategies to manage refractory endometrium: state of the art in 2016.

The endometrium is one of a number of factors involved in achieving optimal outcomes after assisted reproductive treatment. Owing to its "passive" growth following adequate ovarian stimulation, it has received virtually no attention. Only when either endometrial thickness or ultrasonographic pattern seem inadequate have different strategies been assessed to try to improve it, especially in those cases where it seems difficult or impossible to make it grow. The objective of this review is to summarize the different strategies that have been investigated in patients with inadequate endometrium, to attempt to provide solid evidence of therapies that may be beneficial and to move away from empirism. A review of the existing literature was performed by searching MEDLINE, EMBASE, Cochrane library and Web of Science for publications in English related to refractory endometrium. Most current treatments are based on anecdotal cases and not on solid data, although worldwide many doctors and patients use them. In conclusion, this review found that it is not easy to provide a pragmatic, evidence-based approach to help physicians and patients confused by the available data on how to improve a poor endometrium. Honest balanced information provided to our patients is the best that we can do

A Zika virus mutation enhances transmission potential and confers escape from protective dengue virus immunity.

Zika virus (ZIKV) and dengue virus (DENV) are arthropod-borne pathogenic flaviviruses that co-circulate in many countries. To understand some of the pressures that influence ZIKV evolution, we mimic the natural transmission cycle by repeating serial passaging of ZIKV through cultured mosquito cells and either DENV-naive or DENV-immune mice. Compared with wild-type ZIKV, the strains passaged under both conditions exhibit increased pathogenesis in DENV-immune mice. Application of reverse genetics identifies an isoleucine-to-valine mutation (I39V) in the NS2B proteins of both passaged strains that confers enhanced fitness and escape from pre-existing DENV immunity. Introduction of I39V or I39T, a naturally occurring homologous mutation detected in recent ZIKV isolates, increases the replication of wild-type ZIKV in human neuronal precursor cells and laboratory-raised mosquitoes. Our data indicate that ZIKV strains with enhanced transmissibility and pathogenicity can emerge in DENV-naive or -immune settings, and that NS2B-I39 mutants may represent ZIKV variants of interest

Human coronavirus OC43-elicited CD4+ T cells protect against SARS-CoV-2 in HLA transgenic mice

Abstract SARS-CoV-2-reactive T cells are detected in some healthy unexposed individuals. Human studies indicate these T cells could be elicited by the common cold coronavirus OC43. To directly test this assumption and define the role of OC43-elicited T cells that are cross-reactive with SARS-CoV-2, we develop a model of sequential infections with OC43 followed by SARS-CoV-2 in HLA-B*0702 and HLA-DRB1*0101 Ifnar1 −/− transgenic mice. We find that OC43 infection can elicit polyfunctional CD8+ and CD4+ effector T cells that cross-react with SARS-CoV-2 peptides. Furthermore, pre-exposure to OC43 reduces subsequent SARS-CoV-2 infection and disease in the lung for a short-term in HLA-DRB1*0101 Ifnar1 −/− transgenic mice, and a longer-term in HLA-B*0702 Ifnar1 −/− transgenic mice. Depletion of CD4+ T cells in HLA-DRB1*0101 Ifnar1 −/− transgenic mice with prior OC43 exposure results in increased viral burden in the lung but no change in virus-induced lung damage following infection with SARS-CoV-2 (versus CD4+ T cell-sufficient mice), demonstrating that the OC43-elicited SARS-CoV-2 cross-reactive T cell-mediated cross-protection against SARS-CoV-2 is partially dependent on CD4+ T cells. These findings contribute to our understanding of the origin of pre-existing SARS-CoV-2-reactive T cells and their effects on SARS-CoV-2 clinical outcomes, and also carry implications for development of broadly protective betacoronavirus vaccines