Evaluation of DNA Single and Double Strand Breaks in Women with Cervical Neoplasia Based on Alkaline and Neutral Comet Assay Techniques

A hospital-based unmatched case-control study was performed in order to determine the relation of DNA single (ssb) and double (dsb) strand breaks in women with and without cervical neoplasia. Cervical epithelial cells of 30 women: 10 with low grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 without cervical lesions were evaluated using alkaline and neutral comet assays. A significant increase in global DNA damage (ssb + dsb) and dsb was observed in patients with HG-SIL (48.90 ± 12.87 and 23.50 ± 13.91), patients with LG-SIL (33.60 ± 14.96 and 11.20 ± 5.71), and controls (21.70 ± 11.87 and 5.30 ± 5.38; resp.). Pearson correlation coefficient reveled a strong relation between the levels ssb and dsb (2=0.99, =0.03, and 2=0.94, =0.16, resp.) and progression of neoplasia. The increase of dsb damage in patients with HG-SIL was confirmed by DNA breakage detection-FISH (DBD-FISH) on neutral comets. Our results argue in favor of a real genomic instability in women with cervical neoplasia, which was strengthened by our finding of a higher proportion of DNA dsb

Una aproximación estocástica al impacto de los inventarios en las empresas comercializadoras de izúcar de matamoros mediante análisis del indicador dupont y el punto de equilibrio.

Este trabajo cuantifica el impacto de la eficiencia –medida por la rotación de activos y la gestión de inventarios-, el margen de utilidad y el apalancamiento financiero sobre el desempeño del indicador de rentabilidad del patrimonio –ROE– de las empresas comercializadoras de la región de Izúcar de Matamoros, haciendo uso de un análisis Dupont estocástico y de la determinación del punto de equilibrio en días para los inventarios. Se utiliza la Simulación de Montecarlo –SM– para modelar el sistema Dupont y el punto de equilibrio, aproximando todas las cifras del Balance General –BG- y el Estado de Resultados –PyG– a las distribuciones de probabilidad de mejor ajuste y determinar una cifra para explicar el rendimiento del patrimonio y de la inversión de este tipo de empresas y determinando el punto de equilibrio en días para los inventarios. Luego, a través de un análisis de sensibilidad mediante @Risk, se determina el impacto de las variables explicativas de eficiencia, margen y el apalancamiento financiero en el comportamiento del Índice Dupont proyectado y del punto de equilibrio y por último se realiza una caracterización de la cultura del control de inventarios por medio de una encuesta a 152 Pymes de la región ABSTRACT This paper quantifies the impact of management efficiency of commercial agencies in the region of Izúcar of Matamoros, measured by the inventories and assets turn-over, profit margin and financial leveraging on the performance metric return on equity -ROE-. Using a stochastic Dupont analysis and assessment inventories’ breakeven in days. -SM- Monte Carlo Simulation is used to model the Dupont system and breakeven. Using information from outside the general double entry book-keeping accounting system and Income Statement to in order to find the best fit’s probability distributions and determine a metric to explain the equity and investment’s performance of these businesses and determining the inventories’ breakeven in days. Then, through a sensitivity analysis using Risk, the behavior of Dupont index is determined using the explanatory variables of efficiency, margin and leverage effects and breakeven is determined

Long term follow-up of a full- arch rehabilitation with an implant-supported overdenture on four zirconia implants and a zirconia bar. A case report

This clinical report describes a mandibular full-arch rehabilitation by means of an implant-supported overdenture on four zirconia implants. A female patient with an edentulous mandibular arch attended our dental clinic seeking a metal-free mandibular re

Experimental Observation of Flat Bands in One-Dimensional Chiral Magnonic Crystals

Spin waves represent the collective excitations of themagnetizationfield within a magnetic material, providing dispersion curves thatcan be manipulated by material design and external stimuli. Bulk andsurface spin waves can be excited in a thin film with positive ornegative group velocities and, by incorporating a symmetry-breakingmechanism, magnetochiral features arise. Here we study the band diagramof a chiral magnonic crystal consisting of a ferromagnetic film incorporatinga periodic Dzyaloshinskii-Moriya coupling via interfacial contactwith an array of heavy-metal nanowires. We provide experimental evidencefor a strong asymmetry of the spin wave amplitude induced by the modulatedinterfacial Dzyaloshinskii-Moriya interaction, which generatesa nonreciprocal propagation. Moreover, we observe the formation offlat spin-wave bands at low frequencies in the band diagram. Calculationsreveal that depending on the perpendicular anisotropy, the spin-wavelocalization associated with the flat modes occurs in the zones withor without Dzyaloshinskii-Moriya interaction

Tejiendo diálogos. Reflexiones contemporáneas sobre la expresión y el sentido

El presente libro se divide en tres grandes bloques temáticos que contienen a los conceptos guía del título: Complejidad, expresión y sentido y, se organizan de acuerdo con lo que podría considerarse manifestaciones contextuales de las relaciones complejas y manifestaciones de sentido, que emergen como organizadoras a partir de diferentes formas en las que se manifiesta el diseño, las artes y la comunicación, El primer bloque obedece al principio dialógico de la no exclusión en donde que reza: “A no puede ser A y no A” y se titula: Complejidad y expresiones de la cultura contemporánea componiéndose por cinco textos que tocan dimensiones problemáticas en contextos contemporáneos como la intervención urbana y el feminismo como diálogo a través de la imagen y la educación; los discursos de odio como emergencia y la aparición de nuevas necesidades de formación universitaria ante ámbitos de competencia complejos. La primera sección titulada Condiciones emergentes de organización y sentido se presentan los textos en los que la expresión y el sentido aparecen como formas específicas a través de los cuáles es posible no solo asegurar las fronteras de operaciones sociales sino proyectarlas hacia nuevas condiciones de organización y sentido como sería el mundo de la virtualidad. En la segunda sección compuesta por cinco ensayos que de algún modo coinciden en la importancia de los puntos de conexión e interacciones en los fenómenos complejos como inductores de orden-desorden-complejidad, ponen de manifiesto su papel como productores de relaciones que permiten dar cuenta de la incertidumbre, comprender y exponer acontecimientos a nivel educativo, social y artístico. Finalmente en la tercera sección, el bloque que cierra el contenido del libro, se compone por cinco textos que dentro de su argumentación podrían verse alineados con el postulado de Nicolescu del Tercero incluido en donde A no puede ser A y a la vez existe no A, aludiendo a dimensiones problemáticas en contextos contemporáneos como: la intervención urbana y el feminismo como diálogo a través de la imagen y la educación; los discursos de odio como emergencia y la aparición de nuevas necesidades de formación universitaria ante ámbitos de competencia complejos.Olivia Fragoso Susunaga, María Teresa Olalde Ramos y Gustavo Garduño Oropeza, coordinadores

Interacciones semióticas entre el diseño, el arte y la cultura

260 páginasSe expone la forma en la que, el arte, el diseño y la cultura encontraron convergencia en el planteamiento de los estudios actuales sobre la semiótica de la imagen. En sus capítulos se muestran caminos en donde el signo, la significación y el sentido se entretejen como parte de la semiótica de la cultura, de la representación simbólica y la intertextualidad, buscando enriquecer el estudio del funcionamiento y operación de los procesos de significación, desde una visión donde la interdisciplinariedad se expone como el eje que orienta la interacción de los signos que son objeto de este volumen. Los diferentes puntos de vista aquí reunidos han sido estructurados en tres secciones, tituladas: abordajes semióticos, imágenes y representaciones y el juego de los signos, en donde se hace patente la relevancia de realizar aproximaciones interdisciplinarias que sirven para analizar los procesos culturales como fenómenos sígnicos articulados, los cuales trascienden a la esfera antropológica.María Teresa Olalde Ramos; Claudia Fragoso Susunaga; Olivia Fragoso Susunaga; Consuelo Córdoba Flores, coordinadora

The genetic ancestry of American Creole cattle inferred from uniparental and autosomal genetic markers

Cattle imported from the Iberian Peninsula spread throughout America in the early years of discovery and colonization to originate Creole breeds, which adapted to a wide diversity of environments and later received influences from other origins, including zebu cattle in more recent years. We analyzed uniparental genetic markers and autosomal microsatellites in DNA samples from 114 cattle breeds distributed worldwide, including 40 Creole breeds representing the whole American continent, and samples from the Iberian Peninsula, British islands, Continental Europe, Africa and American zebu. We show that Creole breeds differ considerably from each other, and most have their own identity or group with others from neighboring regions. Results with mtDNA indicate that T1c-lineages are rare in Iberia but common in Africa and are well represented in Creoles from Brazil and Colombia, lending support to a direct African influence on Creoles. This is reinforced by the sharing of a unique Y-haplotype between cattle from Mozambique and Creoles from Argentina. Autosomal microsatellites indicate that Creoles occupy an intermediate position between African and European breeds, and some Creoles show a clear Iberian signature. Our results confirm the mixed ancestry of American Creole cattle and the role that African cattle have played in their development

Marine Biodiversity in the Caribbean: Regional Estimates and Distribution Patterns

This paper provides an analysis of the distribution patterns of marine biodiversity and summarizes the major activities of the Census of Marine Life program in the Caribbean region. The coastal Caribbean region is a large marine ecosystem (LME) characterized by coral reefs, mangroves, and seagrasses, but including other environments, such as sandy beaches and rocky shores. These tropical ecosystems incorporate a high diversity of associated flora and fauna, and the nations that border the Caribbean collectively encompass a major global marine biodiversity hot spot. We analyze the state of knowledge of marine biodiversity based on the geographic distribution of georeferenced species records and regional taxonomic lists. A total of 12,046 marine species are reported in this paper for the Caribbean region. These include representatives from 31 animal phyla, two plant phyla, one group of Chromista, and three groups of Protoctista. Sampling effort has been greatest in shallow, nearshore waters, where there is relatively good coverage of species records; offshore and deep environments have been less studied. Additionally, we found that the currently accepted classification of marine ecoregions of the Caribbean did not apply for the benthic distributions of five relatively well known taxonomic groups. Coastal species richness tends to concentrate along the Antillean arc (Cuba to the southernmost Antilles) and the northern coast of South America (Venezuela – Colombia), while no pattern can be observed in the deep sea with the available data. Several factors make it impossible to determine the extent to which these distribution patterns accurately reflect the true situation for marine biodiversity in general: (1) highly localized concentrations of collecting effort and a lack of collecting in many areas and ecosystems, (2) high variability among collecting methods, (3) limited taxonomic expertise for many groups, and (4) differing levels of activity in the study of different taxa

Effectiveness of an intervention for improving drug prescription in primary care patients with multimorbidity and polypharmacy:Study protocol of a cluster randomized clinical trial (Multi-PAP project)

This study was funded by the Fondo de Investigaciones Sanitarias ISCIII (Grant Numbers PI15/00276, PI15/00572, PI15/00996), REDISSEC (Project Numbers RD12/0001/0012, RD16/0001/0005), and the European Regional Development Fund ("A way to build Europe").Background: Multimorbidity is associated with negative effects both on people's health and on healthcare systems. A key problem linked to multimorbidity is polypharmacy, which in turn is associated with increased risk of partly preventable adverse effects, including mortality. The Ariadne principles describe a model of care based on a thorough assessment of diseases, treatments (and potential interactions), clinical status, context and preferences of patients with multimorbidity, with the aim of prioritizing and sharing realistic treatment goals that guide an individualized management. The aim of this study is to evaluate the effectiveness of a complex intervention that implements the Ariadne principles in a population of young-old patients with multimorbidity and polypharmacy. The intervention seeks to improve the appropriateness of prescribing in primary care (PC), as measured by the medication appropriateness index (MAI) score at 6 and 12months, as compared with usual care. Methods/Design: Design:pragmatic cluster randomized clinical trial. Unit of randomization: family physician (FP). Unit of analysis: patient. Scope: PC health centres in three autonomous communities: Aragon, Madrid, and Andalusia (Spain). Population: patients aged 65-74years with multimorbidity (≥3 chronic diseases) and polypharmacy (≥5 drugs prescribed in ≥3months). Sample size: n=400 (200 per study arm). Intervention: complex intervention based on the implementation of the Ariadne principles with two components: (1) FP training and (2) FP-patient interview. Outcomes: MAI score, health services use, quality of life (Euroqol 5D-5L), pharmacotherapy and adherence to treatment (Morisky-Green, Haynes-Sackett), and clinical and socio-demographic variables. Statistical analysis: primary outcome is the difference in MAI score between T0 and T1 and corresponding 95% confidence interval. Adjustment for confounding factors will be performed by multilevel analysis. All analyses will be carried out in accordance with the intention-to-treat principle. Discussion: It is essential to provide evidence concerning interventions on PC patients with polypharmacy and multimorbidity, conducted in the context of routine clinical practice, and involving young-old patients with significant potential for preventing negative health outcomes. Trial registration: Clinicaltrials.gov, NCT02866799Publisher PDFPeer reviewe

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality