Jumping-based asymmetries are negatively associated with jump, change of direction, and repeated sprint performance, but not linear speed, in adolescent handball athletes

The aim of the present study was to determine the association of multi-directional jumping asymmetries with measures of physical performance. Forty-two youth handball athletes (age: 16.0 ± 1.3 years; body height: 174.11 ± 7.3 cm; body mass: 70.49 ± 13.3 kg) performed a mid-season fitness test battery consisting of single leg countermovement, lateral and broad jump tests, two change of direction speed (CODS) tests, an 8 x 10 m repeated sprint test, and a 20 m sprint. The Kappa coefficient showed only ‘slight’ levels of agreement (K range = -0.05 to 0.15), indicating that asymmetries rarely favoured the same side during each of the jump tests. The single leg countermovement jump showed significantly (p = 0.006) larger asymmetries (11.2 ± 8.4) than the broad jump (6.4 ± 4.6) and significant correlations were present between jumping asymmetries and jump (r = -0.32 to -0.52), CODS (r = 0.31 to 0.32) and repeated sprint (r = 0.35 to 0.40) performance. The findings of the present study highlight the independent nature of jumping asymmetries and associations with measures of physical performance. Practitioners are encouraged to use multiple tests to detect existing side differences and consider appropriate training interventions for the reduction of inter-limb asymmetries

IUCN Red List of Ecosystems

We begin by briefly examining the achievements of the IUCN Red List of Threatened Species, and offering it as the model and motivator for the creation of the IUCN Red List of Ecosystems (RLE). The history of the RLE concept within IUCN is briefly summarized, from the first attempt to formally establish an RLE in 1996 to the present. Major activities since 2008, when the World Conservation Congress initiated a “consultation process for the development, implementation and monitoring of a global standard for the assessment of ecosystem status, applicable at local, regional and global levels,” have included: development of a research agenda for strengthening the scientific foundations of the RLE, publication of preliminary categories and criteria for examination by the scientific and conservation community, dissemination of the effort widely by presenting it at workshops and conferences around the world, and encouraging tests of the system for a diversity of ecosystem types and in a variety of institutional settings. Between 2009 and 2012, the Red List of Ecosystems Thematic Group of the IUCN Commission on Ecosystem Management organized 18 workshops and delivered 17 conferences in 20 countries on 5 continents, directly reaching hundreds of participants. Our vision for the future includes the integration of the RLE to the other three key IUCN knowledge products (IUCN Red List of Threatened Species, World Database on Protected Areas and Key Biodiversity Areas), in an on-line, user-driven, freely-accessible information management system for performing biodiversity assessments. In addition we wish to pilot the integration of the RLE into land/water use planning and macro-economic planning. Fundamental challenges for the future include: substantial expansion in existing institutional and technical capacity (especially in biodiversity-rich countries in the developing world), progressive assessment of the status of all terrestrial, freshwater, marine and subterranean ecosystems, and development of a map of the ecosystems of the world. Our ultimate goal is that national, regional and global RLEs are used to inform conservation and land/water use decision-making by all sectors of society

Monitoreo de procesos de ecológicos del Bosque Seco Tropical: Aplicaciones de sensores remotos para estimaciones a nivel de paisaje y el cambio global

Proyecto de Investigación. y extensión (Código VIE: 5402-1401-1012) Instituto Tecnológico de Costa Rica. Vicerrectoría de Investigación y Extensión (VIE). Escuela de Ingeniería Forestal; Alberta University, Canadá; University of Wisconsin; University of Virginia; Universidad Nacional Autónoma de México; Instituto Venezolano de Investigaciones Científicas; Agencia de Medio Ambiente. Cuba; Universidad Estatal de Belo Horizonte. Brasil; Universidad de Montes Claros. Brasil; Universidad de Pernambuco. Brasil; Universidad Estatal de Río de Janeiro. Brasil, 2014Este proyecto se encadenó al proyecto ya concluido "Dimensiones humanas, ecológicas y biofísicas de los bosques secos tropicales (código VIE 5402-1401-9001)". Durante su ejecución fue posible dar continuidad al análisis de datos y preparación de publicaciones científicas y académicas pendientes como fenología, dinámica de hojarasca, tasas de crecimiento, mortalidad y reingreso en el bosque seco tropical. También el proyecto trabajo en la preparación de artículos que integran los resultados de tres países: México, Costa Rica y Brasil. Así mismo se continuó con las mediciones de campo de las parcelas y procesos ecológicos para crear una base de datos robusta, actualmente y después de 10 años, estas bases permiten modelar los efectos de cambios climáticos en el comportamiento de los procesos ecológicos de los bosques secos tropicales (Bst). El elemento novedoso de este proyecto fue la instalación de torres de fenología por medio de financiamiento externo y que miden sobre el dosel del bosques seco tropical los cambios temporales de la radiación de onda corta y onda larga, tanto de entrada como de salida. Los cambios en la reflectancia de las hojas se relacionan tanto con el ángulo del sol (que podemos corregir automáticamente) como con la biomasa de hojas. Las torres permiten la colección de datos de campo a cada 15 minutos y estos datos se pueden calibrar con los índices de reflectancia de varios sensores espaciales. Una buena calibración para los diferentes tipos de bosques secos tropicales (las torres operan en Brasil, México y Costa Rica) puede permitir hacer simulaciones del efecto del cambio climático en la productividad de los bosques, su impacto en el ciclo del agua y por ende en las capacidades de fijar carbono. Además, el proyecto fue capaz de instalar una torre con equipo altamente especializado que permite determinar los flujos de CO2 y de vapor de agua del bosque seco tropical mediante la técnica de Covarianza de Flujos Turbulentos. Asociada a esta torre de 22 metros se estableció una parcela de una hectárea que permite caracterizar la huella de CO2 del ecosistema de forma más precisa. Esta hectárea permitirá mejorar la clasificación de imágenes satelitales y aéreas, así como también facilitara el uso de sensores remotos y otras técnicas de teledetección. Desde la perspectiva de los recursos naturales, se aplicó un protocolo comprensivo y estandarizado para reconocer y comparar las clasificaciones espaciales de los Bst, la estructura y composición florística de sus etapas sucesionales, dinámica de la hojarasca, fenología y tasas de crecimiento. Desde la perspectiva de las ciencias sociales, el trabajo que se realizó fue innovador dado que se crearon vínculos con el gobierno, los científicos y las comunidades. Se estudió las interacciones de las comunidades vecinas con los Bst, así como los factores socioeconómicos, políticos y legales que controlan su conservación y manejo. El proyecto fue capaz de generar la siguiente producción académica y científica: 9 artículos indexados, 2 artículos en español, 5 capítulos en libros en inglés, 1 capitulo en un enciclopedia, 20 ponencias en evento nacional, 6 ponencias en eventos internacionales, 10 artículos en borrador, 5 Tesis de pregrado y 2 Tesis de Doctorado. En medios de comunicación, el proyecto decidió ser más austero debido al alto valor de los equipos instalados en el Parque Nacional Santa Rosa para así no llamar la atención para provocar los robos o daños, sin embargo fue posible generar 7 publicaciones en medios impresos/Web y una intervención en radio nacional. En cuanto a la participación de talleres de incidencia política fueron organizados 3 talleres con decisores sobre el estado de los bosques secos en Costa Rica y sobre el potencial de trabajo conjunto entre organizaciones y el proyecto de investigación. Además se organizó una sesión de Simposio en el Congreso indexado de la ATBC y la OET en el año 2013. Adicionalmente se participó en otros 5 talleres organizados por otras instituciones. En total el proyecto recibió y entrenó a 29 estudiantes, de los cuales 9 fueron extranjeros y 20 del TEC, los cuales trabajaron como asistentes de laboratorio y campo entrenándose en el uso de instrumentos, montaje y medición de diseños experimentales, colección y procesamiento de datos y muestras. Actualmente se cuenta con una base de datos de crecimiento de 10 años, así como 4 años de datos de torres de fenología y 1 año y 3 meses de datos de flujos de carbono y vapor de agua, los cuales continuaran siendo analizados en la siguiente etapa del proyecto (código VIE 5402-1401-1028).Instituto Tecnológico de Costa Rica

Online carbohydrate 3D structure validation with the Privateer web app

Owing to the difficulties associated with working with carbohydrates, validating glycan 3D structures prior to deposition into the Protein Data Bank has become a staple of the structure-solution pipeline. The Privateer software provides integrative methods for the validation, analysis, refinement and graphical representation of 3D atomic structures of glycans, both as ligands and as protein modifiers. While Privateer is free software, it requires users to install any of the structural biology software suites that support it or to build it from source code. Here, the Privateer web app is presented, which is always up to date and available to be used online (https://privateer.york.ac.uk) without installation. This self-updating tool, which runs locally on the user's machine, will allow structural biologists to simply and quickly analyse carbohydrate ligands and protein glycosylation from a web browser whilst retaining all confidential information on their devices

Landscape-scale benefits of protected areas for tropical biodiversity

We are indebted to numerous local communities, PA and government agency staff, research assistants, and other partners for supporting the field data collection. Research permissions were granted by appropriate forestry and conservation government departments in each country. Special thanks is given to the Sarawak State Government, Sarawak Forestry Corporation, Forest Department Sarawak, Sabah Biodiversity Centre, the Danum Valley Management Committee, the Forest Research Institute Malaysia (FRIM), the Smithsonian Institute and the Tropical Ecology Assessment and Monitoring (TEAM) network, Sarayudh Bunyavejchewin, and Ronglarp Sukmasuang. Support was provided by the United Nations Development Programme, NASA grants NNL15AA03C and 80NSSC21K0189, National Geographic Society’s Committee for the Research and Exploration award #9384–13, the Australian Research Council Discovery Early Career Researcher Award DECRA #DE210101440, the Universiti Malaysia Sarawak, the Ministry of Higher Education Malaysia, Nanyang Technological University Singapore, the Darwin Initiative, Liebniz-IZW, and the Universities of Aberdeen, British Columbia, Montana, and Queensland.Peer reviewedPostprin

Marco activo de recursos de innovación docente: Madrid

Una guía de espacios e instituciones para actividades educativas complementarias en enseñanza secundaria y Formación Profesional

Amphioxus functional genomics and the origins of vertebrate gene regulation.

Vertebrates have greatly elaborated the basic chordate body plan and evolved highly distinctive genomes that have been sculpted by two whole-genome duplications. Here we sequence the genome of the Mediterranean amphioxus (Branchiostoma lanceolatum) and characterize DNA methylation, chromatin accessibility, histone modifications and transcriptomes across multiple developmental stages and adult tissues to investigate the evolution of the regulation of the chordate genome. Comparisons with vertebrates identify an intermediate stage in the evolution of differentially methylated enhancers, and a high conservation of gene expression and its cis-regulatory logic between amphioxus and vertebrates that occurs maximally at an earlier mid-embryonic phylotypic period. We analyse regulatory evolution after whole-genome duplications, and find that-in vertebrates-over 80% of broadly expressed gene families with multiple paralogues derived from whole-genome duplications have members that restricted their ancestral expression, and underwent specialization rather than subfunctionalization. Counter-intuitively, paralogues that restricted their expression increased the complexity of their regulatory landscapes. These data pave the way for a better understanding of the regulatory principles that underlie key vertebrate innovations

The CCP4 suite: integrative software for macromolecular crystallography

The Collaborative Computational Project No. 4 (CCP4) is a UK-led international collective with a mission to develop, test, distribute and promote software for macromolecular crystallography. The CCP4 suite is a multiplatform collection of programs brought together by familiar execution routines, a set of common libraries and graphical interfaces. The CCP4 suite has experienced several considerable changes since its last reference article, involving new infrastructure, original programs and graphical interfaces. This article, which is intended as a general literature citation for the use of the CCP4 software suite in structure determination, will guide the reader through such transformations, offering a general overview of the new features and outlining future developments. As such, it aims to highlight the individual programs that comprise the suite and to provide the latest references to them for perusal by crystallographers around the world.Jon Agirre is a Royal Society University Research Fellow (UF160039 and URF\R\221006). Mihaela Atanasova is funded by the UK Engineering and Physical Sciences Research Council (EPSRC; EP/R513386/1). Haroldas Bagdonas is funded by The Royal Society (RGF/R1/181006). Jose´ Javier Burgos-Ma´rmol and Daniel J. Rigden are supported by the BBSRC (BB/S007105/1). Robbie P. Joosten is funded by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 871037 (iNEXTDiscovery) and by CCP4. This work was supported by the Medical Research Council as part of United Kingdom Research and Innovation, also known as UK Research and Innovation: MRC file reference No. MC_UP_A025_1012 to Garib N. Murshudov, which also funded Keitaro Yamashita, Paul Emsley and Fei Long. Robert A. Nicholls is funded by the BBSRC (BB/S007083/1). Soon Wen Hoh is funded by the BBSRC (BB/T012935/1). Kevin D. Cowtan and Paul S. Bond are funded in part by the BBSRC (BB/S005099/1). John Berrisford and Sameer Velankar thank the European Molecular Biology Laboratory–European Bioinformatics Institute, who supported this work. Andrea Thorn was supported in the development of AUSPEX by the German Federal Ministry of Education and Research (05K19WWA and 05K22GU5) and by Deutsche Forschungsgemeinschaft (TH2135/2-1). Petr Kolenko and Martin Maly´ are funded by the MEYS CR (CZ.02.1.01/0.0/0.0/16_019/0000778). Martin Maly´ is funded by the Czech Academy of Sciences (86652036) and CCP4/STFC (521862101). Anastassis Perrakis acknowledges funding from iNEXT (grant No. 653706), iNEXT-Discovery (grant No. 871037), West-Life (grant No. 675858) and EOSC-Life (grant No. 824087) funded by the Horizon 2020 program of the European Commission. Robbie P. Joosten has been the recipient of a Veni grant (722.011.011) and a Vidi grant (723.013.003) from the Netherlands Organization for Scientific Research (NWO). Maarten L. Hekkelman, Robbie P. Joosten and Anastassis Perrakis thank the Research High Performance Computing facility of the Netherlands Cancer Institute for providing and maintaining computation resources and acknowledge the institutional grant from the Dutch Cancer Society and the Dutch Ministry of Health, Welfare and Sport. Tarik R. Drevon is funded by the BBSRC (BB/S007040/1). Randy J. Read is supported by a Principal Research Fellowship from the Wellcome Trust (grant 209407/Z/17/Z). Atlanta G. Cook is supported by a Wellcome Trust SRF (200898) and a Wellcome Centre for Cell Biology core grant (203149). Isabel Uso´n acknowledges support from STFC-UK/CCP4: ‘Agreement for the integration of methods into the CCP4 software distribution, ARCIMBOLDO_LOW’ and Spanish MICINN/AEI/FEDER/UE (PID2021-128751NB-I00). Pavol Skubak and Navraj Pannu were funded by the NWO Applied Sciences and Engineering Domain and CCP4 (grant Nos. 13337 and 16219). Bernhard Lohkamp was supported by the Ro¨ntgen A˚ ngstro¨m Cluster (grant 349-2013-597). Nicholas Pearce is currently funded by the SciLifeLab and Wallenberg Data Driven Life Science Program (grant KAW 2020.0239) and has previously been funded by a Veni Fellowship (VI.Veni.192.143) from the Dutch Research Council (NWO), a Long-term EMBO fellowship (ALTF 609-2017) and EPSRC grant EP/G037280/1. David M. Lawson received funding from BBSRC Institute Strategic Programme Grants (BB/P012523/1 and BB/P012574/1). Lucrezia Catapano is the recipient of an STFC/CCP4-funded PhD studentship (Agreement No: 7920 S2 2020 007).Peer reviewe