Report of a Pest Risk Analysis for Hydrocotyle ranunculoides

Hydrocotyle ranunculoides originates from the American continent and was introduced into the EPPO region as an ornamental plant for tropical aquaria and garden ponds, where it is still sold under its correct name, sometimes under other names (H. vulgaris, H. leucocephala, and H. natans which is a synonym of H. ranunculoides). The plant was first recorded as naturalised in the south-east of the UK in the 1980s (Newman, 2003). Naturalisation in the Netherlands and in Belgium was recorded in the last decade of the twentieth century (Baas & Duistermaat, 1999; Baas & Holverda, 1996; Krabben & Rotteveel, 2003; Verloove 2006, Invasive Species in Belgium Website). Deleterious impacts have been reported in these three countries. The species is also recorded in France, Ireland, Italy, Germany (see EPPO, 2009) but several EPPO countries are still free from H. ranunculoides and there are concerns that it may be able to enter and establish in further countries. This PRA assesses the risks of its further introduction into other EPPO countries and its current and predicted impact. An initial EPPO PRA was performed and approved in 2005. After the proposal of listing this species in the Directive 2000/29, the European Food Safety Authority reviewed the initial PRA and made some comments. The initial PRA is therefore revised in the view of the EFSA comments and of information having become available after the initial PRA (EFSA, 2007)

Reproduction of Crassula helmsii by seed in western Europe

The amphibious plant species Crassula helmsii is a widely established and still-spreading alien in various parts of Europe, where it is considered invasive as its dense swards stress the viability of local biota. The species was considered to exclusively reproduce through vegetative means, until ex situ germination was recorded from a single locality in Belgium. We assessed whether this seed viability holds on a wider scale, by testing 16 populations from The Netherlands, Belgium, northern France, eastern England and northern Germany in a greenhouse germination experiment. Seedlings were observed from all populations but two, and from each of the five countries. Although most fruits were lacking seeds and the inferred germination percentages were overall low, germinable seed numbers are considerable given the high density of flowering stems. An in situ test revealed seeds to make it through normal winter conditions without signs of physical damage and with retention of germinability. Our results suggest that reproduction by seed is a relatively cryptic but widespread phenomenon throughout western Europe. The persistency of seed banks requires further investigation. Nonetheless, these findings already challenge the efficacy of techniques currently applied in C. helmsii control

Pest risk analysis for Myriophyllum heterophyllum

Myriophyllum heterophyllum presents an overall high phytosanitary risk for the EPPO region with a low uncertainty rating. The overall likelihood of M. heterophyllum continuing to enter the EPPO region is high. The plant is imported into the EPPO region as a misidentified aquatic species for aquaria and ponds and is already established in Austria, Belgium, France, Germany, Hungary, the Netherlands, Spain and Switzerland. The risk of entry into other EPPO countries through import is considered high. The risk of the species establishing in additional countries is high as movement through irrigation and river systems acts to connect countries, facilitating spread regionally. Spread may be accelerated by recreational activities in water bodies invaded by the weed. Impacts of the species within the EPPO region are likely to be severe (high score rating), including aquatic plant species displacement, habitat dominance and effects on other aquatic organisms

Pest risk analysis for Alternanthera philoxeroides

Alternanthera philoxeroides presents a high phytosanitary risk for the EPPO region with a low uncertainty rating. A. philoxeroides is already present in the EPPO region in France and Italy. Further spread within and between EPPO countries is considered likely. The overall likelihood of A. philoxeroides continuing to enter the EPPO region is medium. It is not clear how this species entered the EPPO region and there are no clear pathways of further introduction, as the species is not widely traded as an aquarium plant or as any other type of living plant material. There may be confusion with A. sessilis, or other Alternanthera species traded for aquarium, ornamental or food purposes. The risk of the species establishing in other EPPO countries is considered high as movement through irrigation and river systems may act to connect countries, facilitating spread regionally, especially through high energy unstable river systems that may encourage fragmentation. Spread may be significantly accelerated by water based recreational activities. The potential high impact of the species within the EPPO region should be considered similar to that seen in other countries where the species has invaded and become established; i.e. Australia and the southern states of North America. Impacts are likely to be more pronounced in countries and regions where the climate most suited to population, establishment, growth and spread

The prioritisation of a short list of alien plants for risk analysis within the framework of the Regulation (EU) No. 1143/2014

Thirty-seven alien plant species, pre-identified by horizon scanning exercises were prioritised for pest risk analysis (PRA) using a modified version of the EPPO Prioritisation Process designed to be compliant with the EU Regulation 1143/2014. In Stage 1, species were categorised into one of four lists – a Residual List, EU List of Minor Concern, EU Observation List and the EU List of Invasive Alien Plants. Only those species included in the latter proceeded to the risk management stage where their priority for PRA was assessed. Due to medium or high spread potential coupled with high impacts twenty-two species were included in the EU List of Invasive Alien Plants and proceeded to Stage 2. Four species (Ambrosia trifida, Egeria densa, Fallopia baldschuanica and Oxalis pes-caprae) were assigned to the EU Observation List due to moderate or low impacts. Albizia lebbeck, Clematis terniflora, Euonymus japonicus, Lonicera morrowii, Prunus campanulata and Rubus rosifolius were assigned to the residual list due to a current lack of information on impacts. Similarly, Cornus sericea and Hydrilla verticillata were assigned to the Residual List due to unclear taxonomy and uncertainty in native status, respectively. Chromolaena odorata, Cryptostegia grandiflora and Sphagneticola trilobata were assigned to the Residual List as it is unlikely they will establish in the Union under current climatic conditions. In the risk management stage, Euonymus fortunei, Ligustrum sinense and Lonicera maackii were considered a low priority for PRA as they do not exhibit invasive tendencies despite being widely cultivated in the EU over several decades. Nineteen species were identified as having a high priority for a PRA (Acacia dealbata, Ambrosia confertiflora, Andropogon virginicus, Cardiospermum grandiflorum, Celastrus orbiculatus, Cinnamomum camphora, Cortaderia jubata, Ehrharta calycina, Gymnocoronis spilanthoides, Hakea sericea, Humulus scandens, Hygrophila polysperma, Lespedeza cuneata, Lygodium japonicum, Pennisetum setaceum, Prosopis juliflora, Sapium sebiferum, Pistia stratiotes and Salvinia molesta)

Investigation of the association of the CRTM and CRTL1 genes with radiographically evident osteoarthritis in subjects from the Rotterdam study

__Objective:__ To investigate whether radiographically evident osteoarthritis (ROA) in 55-65-year-old men and women is associated with specific alleles or genotypes of the cartilage matrix protein (CRTM) and cartilage link protein (CRTLl) genes. __Methods:__ Cases were selected from a populationbased study on the presence of ROA of the knee or hip. Further radiographic analysis included scoring for spine and hand ROA. Controls, selected from the same population, were free of ROA in all joints. __Results:__ The CRTM locus was significantly associated with hip ROA in men (odds ratio 0.50, 95% confidence interval 0.26-0.95). A significant association between ROA and the CRTLl gene was not observed. __Conclusion:__ These results suggest that the CRTM locus may play a role in the sex- and joint site-specific pattern of ROA development

ARES(2014)2425342 - 22/07/2014. Organisation and running of a scientific workshop to complete selected invasive alien species (IAS) risk assessments

The introduction and spread of invasive alien species (IAS) constitutes one of the most important drivers of global change in biodiversity and ecosystem services. Robust risk assessment methods are required for IAS to provide the foundation upon which to prioritise appropriate action. In a previous study (Roy, Schonrogge et al. 2014) minimum standards were developed to provide an assessment framework for risk assessments and ultimately for underpinning the development of a proposed list of “IAS of EU concern”, in accordance to the provisions of the Regulation (EU) No 1143/2014 of the European Parliament and of the Council of 22 October 2014 on the prevention and management of the introduction and spread of invasive alien species. In practice, of the protocols assessed in detail, only four (GB NNRA, EPPO DSS, Harmonia+ and ENSARS) were sufficiently compliant with the minimum standards to be considered and of these only the GB NNRA and EPPO DSS have published IAS risk assessments. As a result, using the information from such “substantially compliant” protocols, a draft list of approximately 50 species was compiled. It is important to note that this list of species is based on availability of robust risk assessments already completed through methods which are almost compliant with the minimum standards, and it does not constitute the list of “IAS of EU concern”. In view of the application of the forthcoming EU Regulation on IAS (and building-on ENV.B.2/ETU/2013/0026) the Commission hosted a 2-day scientific workshop to examine the selected risk assessments and pool the existing knowledge existing in the EU to complete the missing information, on the basis of robust scientific evidence, in order to make them fully compliant with the minimum standards, wherever possible. The workshop was led by Helen Roy (CEH) and Riccardo Scalera (ISSG). An additional 16 experts from fifteen member states were selected based on their expertise in invasion biology and represented a breadth of expertise from a variety of perspectives including taxonomic (all taxa), environmental (freshwater, marine and terrestrial), impacts (environmental, socio-economic and health) and disciplines (ecologists, conservation practitioners, scientists, policy-makers, risk assessors). In view of the gaps across risk assessments for ecosystem services and climate change two experts were invited to guide the development of approaches for these specific themes. In total the risk assessments for 56 species were considered. The GB NNRA and EPPO DSS have published IAS risk assessments which, when considering species that score medium to high impact, together cover 51 species (noting that Fallopia japonica and F. sachalinensis are separate species). Two further risk assessments were suggested for consideration by the GB Non-Native Species Secretariat which follow the GB NNRA protocol: coati (Nasua nasua) and skunk (Mephitis mephitis), although scored as low impact. Finally an additional three species have been considered through new European–wide risk assessments, with the reported outcome of high impact, for this project which again follow the GB NNRA protocol: Pallas squirrel (Callosciurus erythraeus), grey squirrel (Sciurus carolinensis) and coypu (Myocastor coypus). The main gaps across all risk assessments were in relation to climate change and ecosystem services but additional information was also required on benefits as mentioned with minimum standard “Description (Taxonomy, invasion history, distribution range (native and introduced), geographic scope, socio-economic benefits)” and in some cases information to support the minimum standard “Includes status (threatened or protected) of species or habitat under threat“ was missing. It was agreed that systematic consideration of a list of questions in relation to the minimum standards on ecosystem services and climate change would be useful guidance for experts. An outline of the approaches agreed through the workshop for the minimum standards “Includes possible effects of climate change in the foreseeable future“ and “Can broadly assess environmental impact with respect to ecosystem services” were developed as guidance for documenting information in relation to climate change and ecosystem services. Each species was considered separately with the experts providing an overview of the information available for addressing the identified gaps. After all species had been considered the workshop participants (excluding the EC, Helen Roy and Riccardo Scalera) adopted a consensus approach to confirm whether or not the risk assessment was compliant with the minimum standards and whether the overall score of the risk assessment remained applicable. No changes were made to the scores but any recommendations were noted. There were very few recommendations for change. The outcome for each risk assessment was agreed and summarised as “compliant” or “not compliant” with the minimum standards. Of the risk assessments for the 56 species considered through this project, 53 were agreed to be fully compliant with the minimum standards. However, Pacific oyster, Crassostrea gigas, although compliant with the minimum standards should be excluded as it is not within the scope of the regulation (see art 2.e) because it is listed in annex IV of Council Regulation (EC) No 708/2007 of 11 June 2007 concerning use of alien and locally absent species in aquaculture. Four of the risk assessments were not considered to be compliant because of major information gaps: Elodea canadensis (Canadian pondweed), Heracleum mantegazzianum (giant hogweed), M. mephitis (skunk), N. nasua (coati)

Large trees drive forest aboveground biomass variation in moist lowland forests accross the tropics

peer reviewedaudience: researcher, professional, studentAim Large trees (d.b.h. 70 cm) store large amounts of biomass. Several studies suggest that large trees may be vulnerable to changing climate, potentially leading to declining forest biomass storage. Here we determine the importance of large trees for tropical forest biomass storage and explore which intrinsic (species trait) and extrinsic (environment) variables are associated with the density of large trees and forest biomass at continental and pan-tropical scales. Location Pan-tropical. Methods Aboveground biomass (AGB) was calculated for 120 intact lowland moist forest locations. Linear regression was used to calculate variation in AGB explained by the density of large trees. Akaike information criterion weights (AICcwi) were used to calculate averaged correlation coefficients for all possible multiple regression models between AGB/density of large trees and environmental and species trait variables correcting for spatial autocorrelation. Results Density of large trees explained c. 70% of the variation in pan-tropical AGB and was also responsible for significantly lower AGB in Neotropical [287.8 (mean) 105.0 (SD) Mg ha-1] versus Palaeotropical forests (Africa 418.3 91.8 Mg ha-1; Asia 393.3 109.3 Mg ha-1). Pan-tropical variation in density of large trees and AGB was associated with soil coarseness (negative), soil fertility (positive), community wood density (positive) and dominance of wind dispersed species (positive), temperature in the coldest month (negative), temperature in the warmest month (negative) and rainfall in the wettest month (positive), but results were not always consistent among continents. Main conclusions Density of large trees and AGB were significantly associated with climatic variables, indicating that climate change will affect tropical forest biomass storage. Species trait composition will interact with these future biomass changes as they are also affected by a warmer climate. Given the importance of large trees for variation in AGB across the tropics, and their sensitivity to climate change, we emphasize the need for in-depth analyses of the community dynamics of large trees

Maternal Colonization With Group B Streptococcus and Serotype Distribution Worldwide: Systematic Review and Meta-analyses.

Background: Maternal rectovaginal colonization with group B Streptococcus (GBS) is the most common pathway for GBS disease in mother, fetus, and newborn. This article, the second in a series estimating the burden of GBS, aims to determine the prevalence and serotype distribution of GBS colonizing pregnant women worldwide. Methods: We conducted systematic literature reviews (PubMed/Medline, Embase, Latin American and Caribbean Health Sciences Literature [LILACS], World Health Organization Library Information System [WHOLIS], and Scopus), organized Chinese language searches, and sought unpublished data from investigator groups. We applied broad inclusion criteria to maximize data inputs, particularly from low- and middle-income contexts, and then applied new meta-analyses to adjust for studies with less-sensitive sampling and laboratory techniques. We undertook meta-analyses to derive pooled estimates of maternal GBS colonization prevalence at national and regional levels. Results: The dataset regarding colonization included 390 articles, 85 countries, and a total of 299924 pregnant women. Our adjusted estimate for maternal GBS colonization worldwide was 18% (95% confidence interval [CI], 17%-19%), with regional variation (11%-35%), and lower prevalence in Southern Asia (12.5% [95% CI, 10%-15%]) and Eastern Asia (11% [95% CI, 10%-12%]). Bacterial serotypes I-V account for 98% of identified colonizing GBS isolates worldwide. Serotype III, associated with invasive disease, accounts for 25% (95% CI, 23%-28%), but is less frequent in some South American and Asian countries. Serotypes VI-IX are more common in Asia. Conclusions: GBS colonizes pregnant women worldwide, but prevalence and serotype distribution vary, even after adjusting for laboratory methods. Lower GBS maternal colonization prevalence, with less serotype III, may help to explain lower GBS disease incidence in regions such as Asia. High prevalence worldwide, and more serotype data, are relevant to prevention efforts