Самосборка низкоразмерных систем Al, Ni, Ti и Si в условиях околоравновесной конденсации

Дисертація присвячена вивченню закономірностей структуро- утворення тривимірних мікро- та наносистем Al, Ni, Ti та Si за умов близькорівноважної стаціонарної конденсації. Вивчена залежність механізмів структуроутворення конденсату від основних параметрів технологічного процесу: часу конденсації, тиску робочого газу та потужності розряду. Проаналізовані фізичні процеси, що відбуваються під час роботи накопичувальних систем плазма-конденсат. Для цих систем розроблено математичну модель самоорганізації критично малих стаціонарних пересичень, яка вивчена за допомогою фазової площини. Досліджено вплив на кінетику формування мікро- та наносистем Al, Ni, Ti та Si взаємозв’язаних ефекту Гіббса-Томсона та польової селективності. Розроблені та апробовані за допомогою побудови фазових траєкторій математичні моделі, які описують процес самозбірки низькорозмірних систем. Встановлено, що за близько-рівноважних умов можна реалізувати різні структурно-морфологічні форми конденсатів. При цитуванні документа, використовуйте посилання http://essuir.sumdu.edu.ua/handle/123456789/24862Диссертация посвящена изучению закономерностей структуро- образования микро- и наносистем Al, Ni, Ti и Si в условиях критически малых пересыщений. Изучены процессы формирования конденсатов под влиянием структурной и полевой селективностей, степень проявления которых зависит от таких основных технологических параметров, как время конденсации, давление рабочего газа и мощность разряда. Проанализированы конструкции существующих распылитель- ных систем, разработана и создана накопительная система плазма- конденсат (НСПК) на базе планарного магнетрона и совмещенного с ним полого катода. Проанализированы происходящие в НСПК физические процессы и разработана математическая модель, описы- вающая изменение во времени таких нелинейно взаимозависимых технологических параметров, как пересыщение и температура ростовой поверхности. Методом фазовой плоскости установлена возможность самоорганизации критически малых стационарных пресыщений. С помощью математического моделирования показано, что при повышении давления рабочего газа происходит усреднение энергии распыленных атомов, что является необходимым условием повышения стационарности технологического процесса. Изучен механизм формирования фракталов Ni при осаждении предельно слабых паровых потоков на нагретые до относительно высоких температур (620 К) подложки. Показано, что снижение пересыщения паров Ni до критического значения может предельно локализировать области закрепления атомов и является основой для формирования на ростовой поверхности вискеров. Впервые показано, что диссипативная самоорганизация предельно малых стационарных пересыщений в накопительных системах плазма-конденсат приводит к консервативной самоорганизации низкоразмерных систем в виде тех или иных структурно-морфологических состояний поверхности конденсата. Изучены механизмы самосборки пористых пленок Al, трехмерных систем Ti в виде слабо связанных друг с другом ограненных или округлых кристаллов. Показано, что основой такой самосборки конденсатов является предельная минимизация свободной энергии конденсата. При этом селективная самосборка развитой поверхности металлов при проявлении полевой селективности является следствием выравнивания химических потенциалов в различных точках ростовой поверхности до уровня, при котором локальные скорости наращивания конденсатов не зависят от координат поверхности и не изменяются во времени. Изучены механизмы структурообразования конденсатов Si и разработана математическая модель, описывающая самоорганизацию островков одинаковых размеров и формы. При цитировании документа, используйте ссылку http://essuir.sumdu.edu.ua/handle/123456789/24862The aim of dissertation is the study of regularities of Al, Ni, Ti and Si micro- and nanosystems formation under the conditions of critically small supersaturations. It have been studied the processes of condensates forming at influence of structural and field selectivity, they are depend on main technological parameters of condensation process such as time of condensation, pressure of working gas and power discharge. Physical processes in the accumulative ion-plasma system have been analyzed. For this systems was created mathematical model of selforganization extremely low steady-state supersaturation and studied by method of phase plane. The influence of interdependent effects of Gibbs-Thomson and field selectivities on forming kinetic of micro- and nanosystems of Al, Ni, Ti and Si was investigated. It was created and tested due to plotting of phase trajectories mathematical models, which reply for the self-assembly process of low dimensional systems. It was established that different structuralmorphological forms of condensates could be obtain at near equilibrium conditions. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/2486

WDR5, BRCA1, and BARD1 Co-regulate the DNA Damage Response and Modulate the Mesenchymal-to-Epithelial Transition during Early Reprogramming.

Differentiated cells are epigenetically stable, but can be reprogrammed to pluripotency by expression of the OSKM transcription factors. Despite significant effort, relatively little is known about the cellular requirements for reprogramming and how they affect the properties of induced pluripotent stem cells. We have performed high-content screening with small interfering RNAs targeting 300 chromatin-associated factors and extracted colony-level quantitative features. This revealed five morphological phenotypes in early reprogramming, including one displaying large round colonies exhibiting an early block of reprogramming. Using RNA sequencing, we identified transcriptional changes associated with these phenotypes. Furthermore, double knockdown epistasis experiments revealed that BRCA1, BARD1, and WDR5 functionally interact and are required for the DNA damage response. In addition, the mesenchymal-to-epithelial transition is affected in Brca1, Bard1, and Wdr5 knockdowns. Our data provide a resource of chromatin-associated factors in early reprogramming and underline colony morphology as an important high-dimensional readout for reprogramming quality.V.B. and C.B. are funded by the Stand Up to Cancer campaign for Cancer Research UK, and Cancer Research UK Program Foundation Award to C.B. (C37275/1A20146). K.M. was supported by an NWO-VIDI grant (864.12.010)

Neuropeptide S receptor gene - converging evidence for a role in panic disorder

Animal studies have suggested neuropeptide S (NPS) and its receptor (NPSR) to be involved in the pathogenesis of anxiety-related behavior. In this study, a multilevel approach was applied to further elucidate the role of NPS in the etiology of human anxiety. The functional NPSR A/T (Asn¹⁰⁷Ile) variant (rs324981) was investigated for association with (1) panic disorder with and without agoraphobia in two large, independent case-control studies, (2) dimensional anxiety traits, (3) autonomic arousal level during a behavioral avoidance test and (4) brain activation correlates of anxiety-related emotional processing in panic disorder. The more active NPSR rs324981 T allele was found to be associated with panic disorder in the female subgroup of patients in both samples as well as in a meta-analytic approach. The T risk allele was further related to elevated anxiety sensitivity, increased heart rate and higher symptom reports during a behavioral avoidance test as well as decreased activity in the dorsolateral prefrontal, lateral orbitofrontal and anterior cingulate cortex during processing of fearful faces in patients with panic disorder. The present results provide converging evidence for a female-dominant role of NPSR gene variation in panic disorder potentially through heightened autonomic arousal and distorted processing of anxiety-relevant emotional stimuli

Year in review in Intensive Care Medicine 2010: I. Acute renal failure, outcome, risk assessment and ICU performance, sepsis, neuro intensive care and experimentals

SCOPUS: re.jinfo:eu-repo/semantics/publishe

The World Federation of ADHD International Consensus Statement:208 Evidence-based conclusions about the disorder

Background: Misconceptions about ADHD stigmatize affected people, reduce credibility of providers, and prevent/delay treatment. To challenge misconceptions, we curated findings with strong evidence base. Methods: We reviewed studies with more than 2000 participants or meta-analyses from five or more studies or 2000 or more participants. We excluded meta-analyses that did not assess publication bias, except for meta-analyses of prevalence. For network meta-analyses we required comparison adjusted funnel plots. We excluded treatment studies with waiting-list or treatment as usual controls. From this literature, we extracted evidence-based assertions about the disorder. Results: We generated 208 empirically supported statements about ADHD. The status of the included statements as empirically supported is approved by 80 authors from 27 countries and 6 continents. The contents of the manuscript are endorsed by 366 people who have read this document and agree with its contents. Conclusions: Many findings in ADHD are supported by meta-analysis. These allow for firm statements about the nature, course, outcome causes, and treatments for disorders that are useful for reducing misconceptions and stigma.</p

Year in review in Intensive Care Medicine 2010: III. ARDS and ALI, mechanical ventilation, noninvasive ventilation, weaning, endotracheal intubation, lung ultrasound and paediatrics

SCOPUS: re.jinfo:eu-repo/semantics/publishe

Year in review in Intensive Care Medicine, 2008: II. Experimental, acute respiratory failure and ARDS, mechanical ventilation and endotracheal intubation

SCOPUS: re.jinfo:eu-repo/semantics/publishe

Year in review in Intensive Care Medicine 2009: II. Neurology, cardiovascular, experimental, pharmacology and sedation, communication and teaching

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Impact of renal impairment on atrial fibrillation: ESC-EHRA EORP-AF Long-Term General Registry

Background: Atrial fibrillation (AF) and renal impairment share a bidirectional relationship with important pathophysiological interactions. We evaluated the impact of renal impairment in a contemporary cohort of patients with AF. Methods: We utilised the ESC-EHRA EORP-AF Long-Term General Registry. Outcomes were analysed according to renal function by CKD-EPI equation. The primary endpoint was a composite of thromboembolism, major bleeding, acute coronary syndrome and all-cause death. Secondary endpoints were each of these separately including ischaemic stroke, haemorrhagic event, intracranial haemorrhage, cardiovascular death and hospital admission. Results: A total of 9306 patients were included. The distribution of patients with no, mild, moderate and severe renal impairment at baseline were 16.9%, 49.3%, 30% and 3.8%, respectively. AF patients with impaired renal function were older, more likely to be females, had worse cardiac imaging parameters and multiple comorbidities. Among patients with an indication for anticoagulation, prescription of these agents was reduced in those with severe renal impairment, p&nbsp;&lt;.001. Over 24&nbsp;months, impaired renal function was associated with significantly greater incidence of the primary composite outcome and all secondary outcomes. Multivariable Cox regression analysis demonstrated an inverse relationship between eGFR and the primary outcome (HR 1.07 [95% CI, 1.01–1.14] per 10&nbsp;ml/min/1.73&nbsp;m2 decrease), that was most notable in patients with eGFR &lt;30&nbsp;ml/min/1.73&nbsp;m2 (HR 2.21 [95% CI, 1.23–3.99] compared to eGFR ≥90&nbsp;ml/min/1.73&nbsp;m2). Conclusion: A significant proportion of patients with AF suffer from concomitant renal impairment which impacts their overall management. Furthermore, renal impairment is an independent predictor of major adverse events including thromboembolism, major bleeding, acute coronary syndrome and all-cause death in patients with AF

Clinical complexity and impact of the ABC (Atrial fibrillation Better Care) pathway in patients with atrial fibrillation: a report from the ESC-EHRA EURObservational Research Programme in AF General Long-Term Registry

Background: Clinical complexity is increasingly prevalent among patients with atrial fibrillation (AF). The ‘Atrial fibrillation Better Care’ (ABC) pathway approach has been proposed to streamline a more holistic and integrated approach to AF care; however, there are limited data on its usefulness among clinically complex patients. We aim to determine the impact of ABC pathway in a contemporary cohort of clinically complex AF patients. Methods: From the ESC-EHRA EORP-AF General Long-Term Registry, we analysed clinically complex AF patients, defined as the presence of frailty, multimorbidity and/or polypharmacy. A K-medoids cluster analysis was performed to identify different groups of clinical complexity. The impact of an ABC-adherent approach on major outcomes was analysed through Cox-regression analyses and delay of event (DoE) analyses. Results: Among 9966 AF patients included, 8289 (83.1%) were clinically complex. Adherence to the ABC pathway in the clinically complex group reduced the risk of all-cause death (adjusted HR [aHR]: 0.72, 95%CI 0.58–0.91), major adverse cardiovascular events (MACEs; aHR: 0.68, 95%CI 0.52–0.87) and composite outcome (aHR: 0.70, 95%CI: 0.58–0.85). Adherence to the ABC pathway was associated with a significant reduction in the risk of death (aHR: 0.74, 95%CI 0.56–0.98) and composite outcome (aHR: 0.76, 95%CI 0.60–0.96) also in the high-complexity cluster; similar trends were observed for MACEs. In DoE analyses, an ABC-adherent approach resulted in significant gains in event-free survival for all the outcomes investigated in clinically complex patients. Based on absolute risk reduction at 1 year of follow-up, the number needed to treat for ABC pathway adherence was 24 for all-cause death, 31 for MACEs and 20 for the composite outcome. Conclusions: An ABC-adherent approach reduces the risk of major outcomes in clinically complex AF patients. Ensuring adherence to the ABC pathway is essential to improve clinical outcomes among clinically complex AF patients