Adding value to milk by increasing its protein and CLA contents

End of project reportThe mid-summer milk protein study was undertaken on 34 commercial dairy farms in 2005 to evaluate the influence of dietary and management variables on milk protein content in mid-season. Data on grass composition, genetic merit of the herds and milk protein content were collected and analysed by multiple regression. Both calving date and genetic merit for milk protein content were significantly associated with milk protein content and were used as adjustment factors when evaluating the association between measures of grass quality and milk protein content. Milk protein content was associated with grass OMD (P = 0.04) and NDF content (P = 0.02) but not with CP content (P = 0.80). It is concluded that herds calving earlier, with a greater genetic merit for milk protein content and consuming better quality pasture would have greater milk protein contents in mid-season

Evaluation and Refinement of the French Protein System (PDI) under Irish Conditions

End of Project ReportThe CP and DM degradability of grazed grass (between April and October, inclusive) and grass silage samples (differing in cut number and treatment) was determined using the in situ technique and the results obtained were used to calculate the PDIE and PDIN values. The degradability data on 12 concentrate ingredients from a previous study (Woods, 2000) were used to estimate PDIE and PDIN values for these feedstuffs. The mean PDIE and PDIN values determined for grass were 81 g/kg and 127 g/kg DM, respectively and for grass silage were 60 g/kg and 94 g/kg DM. Regression analysis resulted in equations to predict the PDIN of samples of grazed grass (PDIN = 3.8 + 0.628 CP) (R²= 0.999) and grass silage (PDIN = 5.9 + 0.605 CP) (R²= 0.997), and the PDIE of grazed grass (PDIE = 181.4 - 0.104 NDF - 0.195 ash - 0.047 OMD) (R²= 0.987) and grass silage (PDIE = 27.7 + 0.083 DMD - 0.147 CP) (R²= 0.812). The PDIE and PDIN values of 11 of the 12 concentrate ingredients were similar to those used in the French Tables but the maize distillers’ grains in this study and those used in France would appear to be quite different products. The response to PDIE and PDIN in the diet of lactating cows was evaluated and the effect of better balancing the PDIE and PDIN supply on the efficiency of N utilisation was assessed. Twenty autumn calving cows were blocked in a complete Latin Square design and assigned to four different diets varying in PDIN and PDIE content. Each of the four treatments consisted of a concentrate, maize silage and grass silage in the proportions 37:38:25 on a DM basis. There were 4 periods of 4-week duration each. Diet A contained 92 g/kg DM of PDIE and 116 g/kg DM of PDIN. Diet B contained 103 g/kg DM of PDIE and 122 g/kg DM of PDIN. Diet C and D over supplied PDIN at 137 g/kg and 153 g/kg, respectively relative to PDIE at 111 g/kg DM. Dry matter intake increased significantly with the excess dietary PDIN relative to PDIE but there were no significant differences in milk yield and composition. Decreasing the supply of PDIE in the diet (i.e. diet A vs. B) resulted in no significant effect on milk or constituent yields but did significantly reduce the efficiency (kg milk / kg DMI) of milk production. There was also a significant reduction in the efficiency of milk produced per kg DMI with increasing dietary concentrations of PDIN and increasing PDIN: PDIE balance (B>C>D). Increasing the dietary PDIN from 122 to 153 g/kg DMI increased urine N (+54%), faecal N (+11%) and plasma urea concentrations (+75%). The results indicate that the optimum concentration of dietary PDI is approximately 103 g/kg DM for cows producing about 35 kg of milk per day. A better balance between PDIE and PDIN supply improves the efficiency of conversion of DM to milk and dietary protein

Evaluation of fermented whole crop wheat, urea-treated processed whole crop wheat and maize silage for dairy cows

End of Project ReportThere has been increased interest in and increased usage of forages other than grass silage for feeding dairy cows during the winter period. This has arisen because of the inconsistency in making good quality grass silage and the low intake characteristics of this feed. The main objective of this project was to evaluate the effects on dairy cow intake and performance of offering fermented whole crop wheat (WCW) silage, urea-treated processed WCW and maize silage in mixtures with grass silage compared with grass silage alone. The value of these feeds as supplements to grazed grass in the Autumn for late lactation spring calving cows and their effect on dietary nitrogen (N) utilisation for milk protein production were also investigated. The first two experiments evaluated the effects of including 67% of the forage mixture on a dry matter (DM) basis as fermented WCW, urea-treated processed WCW (also known as “Alkalage”) or maize silage in comparison to grass silage alone in the diet of autumn calving cows. The fermented WCW and urea-treated processed WCW were harvested at a stubble height of approximately 20 cm. Sixty and 95% of the grain was milled/cracked in the urea-treated processed WCW in experiments 1 and 2, respectively. Forages were supplemented with concentrates of varying crude protein (CP) concentrations so as to maintain a similar CP concentration in the total dietary dry matter across treatments. In both experiments all the feeds were well preserved. The DM (g/kg) and starch contents (g/kg DM) in the fermented WCW, urea-treated processed WCW and maize silage in experiments 1 were 406 and 282, 733 and 324, 221 and 140 and in experiment 2 were 370 and 323, 763 and 341 and 302 and 324, respectively. Results from both experiments were similar. The three forage mixtures resulted in greater DM intake and greater fat plus protein production than grass silage. The largest effect on intake was obtained with the urea-treated processed WCW which probably reflected the greater DM content of this forage mixture compared with the others. Milk protein content was generally similar across the three forage mixtures and greater than on grass silage as the sole forage. In the third experiment short-straw urea-treated processed WCW (harvested at a stubble height of 35 cm) was evaluated in comparison to the fermented WCW (harvested at a stubble height of 20 cm), maize silage and grass silage. The DM (g/kg) and starch (g/kg DM) contents in the fermented WCW, urea-treated processed WCW and maize silage were 389 and 316, 795 and 382 and 346 and 301, respectively. Fifty nine percent of the grain was cracked/processed in the urea-treated processed UP-WCW .Level of inclusion of forages in the diets was the same as in the first two experiments and total dietary CP was again maintained at a similar concentration across treatments by offering concentrates of varying CP concentrations. The higher harvesting height of the urea-treated processed WCW resulted in a greater starch concentration in this feed in comparison to the first two experiments. The results however were very similar to those obtained in the first two experiments with the forage mixtures again increasing DM intake and fat plus protein yield. Inclusion of the short-straw urea-treated processed WCW did not result in greater fat plus protein production compared with the inclusion of fermented WCW or maize silage. The greatest efficiency of conversion of dietary nitrogen (N) to milk N was achieved with the maize silage mixture and the least efficiency with the urea-treated process WCW mixture. In experiment 4 the nutritive value of fermented WCW, urea-treated processed WCW and maize silage were evaluated in comparison to a concentrate as supplements to grazed grass for spring calving cows in the autumn. A treatment with a high grass allowance of 24 kgDM (> 4 cm)/cow per day was also included while the grass allowance on the supplemented treatments was restricted to 17 kgDM (> 4 cm)/cow per day. All the supplemented treatments and the high grass allowance treatment gave greater milk yields than the unsupplemented restricted grass treatment. The concentrate supplement resulted in the greatest solids corrected milk yield and this was greater than any of the forage supplemented treatments which were not significantly different from one another. In the final experiment the output of N in milk, urine and faeces was measured when grass silage, fermented WCW, urea-treated processed WCW and maize silage were fed as the sole forages plus 6 kg of a concentrate containing 338 g crude protein/kg DM. The proportion of consumed N excreted in urine and faeces was greatest on grass silage and urea-treated processed WCW with fermented WCW and maize silage resulting in the greatest proportion of consumed N being excreted in milk. Overall, the project demonstrated that including either type of WCW or maize silage with grass silage increased fat plus protein production and protein concentration to a similar extent compared to grass silage as the sole forage. All three forages increased DM intake with the greatest increase observed with urea-treated processed WCW. Because of this greater intake resulting in similar fat plus protein production conversion of dietary DM to milk solids was less efficient on urea-treated processed WCW based diets than on fermented WCW or maize silage based diets. Grass silage and urea-treated processed WCW based diets were least N efficient with less dietary N being incorporated into milk N than on fermented WCW or maize silage based diets. Comparing the responses on the forage mixtures relative to one another and to grass silage across experiments 1 to 3 indicates that neither degree of grain processing or harvesting height (within the ranges studied here) are of substantial importance in determining the nutritive value of urea-treated processed WCW. All of the three alternative forages gave similar solids corrected milk yield responses when used as buffer feeds for spring calved cows at pasture in the autumn but these responses were less than 50% of the response to a concentrate supplement

Distribution maps of cetacean and seabird populations in the North‐East Atlantic

1. Distribution maps of cetaceans and seabirds at basin and monthly scales are needed for conservation and marine management. These are usually created from standardized and systematic aerial and vessel surveys, with recorded animal den- sities interpolated across study areas. However, distribution maps at basin and monthly scales have previously not been possible because individual surveys have restricted spatial and temporal coverage. 2. This study develops an alternative approach consisting of: (a) collating diverse survey data to maximize spatial and temporal coverage, (b) using detection func- tions to estimate variation in the surface area covered (km2) among these surveys, standardizing measurements of effort and animal densities, and (c) developing species distribution models (SDM) that overcome issues with heterogeneous and uneven coverage. 3. 2.68 million km of survey data in the North-East Atlantic between 1980 and 2018 were collated and standardized. SDM using Generalized Linear Models and General Estimating Equations in a hurdle approach were developed. Distribution maps were then created for 12 cetacean and 12 seabird species at 10 km and monthly resolution. Qualitative and quantitative assessment indicated good model performance. 4. Synthesis and applications. This study provides the largest ever collation and standardization of diverse survey data for cetaceans and seabirds, and the most comprehensive distribution maps of these taxa in the North-East Atlantic. These distribution maps have numerous applications including the identification of im- portant areas needing protection, and the quantification of overlap between vul- nerable species and anthropogenic activities. This study demonstrates how the analysis of existing and diverse survey data can meet conservation and marine management needs.Versión del editor4,7

Evaluation of sesamum gum as an excipient in matrix tablets

In developing countries modern medicines are often beyond the affordability of the majority of the population. This is due to the reliance on expensive imported raw materials despite the abundance of natural resources which could provide an equivalent or even an improved function. The aim of this study was to investigate the potential of sesamum gum (SG) extracted from the leaves of Sesamum radiatum (readily cultivated in sub-Saharan Africa) as a matrix former. Directly compressed matrix tablets were prepared from the extract and compared with similar matrices of HPMC (K4M) using theophylline as a model water soluble drug. The compaction, swelling, erosion and drug release from the matrices were studied in deionized water, 0.1 N HCl (pH 1.2) and phosphate buffer (pH 6.8) using USP apparatus II. The data from the swelling, erosion and drug release studies were also fitted into the respective mathematical models. Results showed that the matrices underwent a combination of swelling and erosion, with the swelling action being controlled by the rate of hydration in the medium. SG also controlled the release of theophylline similar to the HPMC and therefore may have use as an alternative excipient in regions where Sesamum radiatum can be easily cultivated

The Physics of Star Cluster Formation and Evolution

© 2020 Springer-Verlag. The final publication is available at Springer via https://doi.org/10.1007/s11214-020-00689-4.Star clusters form in dense, hierarchically collapsing gas clouds. Bulk kinetic energy is transformed to turbulence with stars forming from cores fed by filaments. In the most compact regions, stellar feedback is least effective in removing the gas and stars may form very efficiently. These are also the regions where, in high-mass clusters, ejecta from some kind of high-mass stars are effectively captured during the formation phase of some of the low mass stars and effectively channeled into the latter to form multiple populations. Star formation epochs in star clusters are generally set by gas flows that determine the abundance of gas in the cluster. We argue that there is likely only one star formation epoch after which clusters remain essentially clear of gas by cluster winds. Collisional dynamics is important in this phase leading to core collapse, expansion and eventual dispersion of every cluster. We review recent developments in the field with a focus on theoretical work.Peer reviewe

Age at first birth in women is genetically associated with increased risk of schizophrenia

Prof. Paunio on PGC:n jäsenPrevious studies have shown an increased risk for mental health problems in children born to both younger and older parents compared to children of average-aged parents. We previously used a novel design to reveal a latent mechanism of genetic association between schizophrenia and age at first birth in women (AFB). Here, we use independent data from the UK Biobank (N = 38,892) to replicate the finding of an association between predicted genetic risk of schizophrenia and AFB in women, and to estimate the genetic correlation between schizophrenia and AFB in women stratified into younger and older groups. We find evidence for an association between predicted genetic risk of schizophrenia and AFB in women (P-value = 1.12E-05), and we show genetic heterogeneity between younger and older AFB groups (P-value = 3.45E-03). The genetic correlation between schizophrenia and AFB in the younger AFB group is -0.16 (SE = 0.04) while that between schizophrenia and AFB in the older AFB group is 0.14 (SE = 0.08). Our results suggest that early, and perhaps also late, age at first birth in women is associated with increased genetic risk for schizophrenia in the UK Biobank sample. These findings contribute new insights into factors contributing to the complex bio-social risk architecture underpinning the association between parental age and offspring mental health.Peer reviewe