2,148 research outputs found
Micropropagación de Ulmus minor y U. minor x U. pumila a partir de ramets de 4 años de edad
As part of the Spanish breeding programme against Dutch elm disease, investigations were undertaken on reproduction of Ulmus minor by means of tissue culture. Microshoots were obtained from nodal segments, collected from terminal twigs of 4-year-old ramets of selected clones of this species and of U. minor x U. pumila, and cultured on a modified Murashige & Skoog medium containing 6-Benzylaminopurine (BA). When subcultured, the microshoots or nodal segments excised from these, formed new shoots, their maximum numbers per explant (1.94 to 2) being obtained with the hybrid clone M-TC2 on media containing 3.5 to 8.8 μM BA. The number of microshoots obtained was highest with subcultures of the whole shoot, e.g. 6.9 times superior to control, against 1.5 with nodal segments of VJR1 in presence of 8.8 μM BA. The addition of 0.5 to 5.3 μM indole-acetic acid of α-naphthaleneacetic acid to the medium induced the highest percentage of root formation from the shoots. Both shoot and root formation varied greatly among clones.En el presente trabajo se describen los trabajos sobre reproducción de Ulmus minor mediante las técnicas de cultivo de tejidos, llevados a cabo en el marco del Programa de Mejora Genética Español de los Olmos Frente a la Grafiosis. Brotes obtenidos a partir de segmentos nodales de ramillos terminales de ramets de 4 años de edad, pertenecientes a clones seleccionados de esta especie y de U. minor x U. pumila, fueron cultivados en el medio de Murashige & Skoog modificado conteniendo 6-Bencilaminopurina (BA). Una vez subcultivados los brotes enteros o los segmentos nodales obtenidos a partir del material vegetal in vitro, se originaron nuevos brotes, y fue el clon híbrido MTC2 el que proporcionó un mayor número por explanto (1,94 a 2) sobre medio conteniendo 3,5 a 8,8 μM BA. El número de brotes obtenidos fue mayor cuando todo el brote fue subcultivado, e.g. 6,9 veces superior al control, frente a 1,5 con segmentos nodales de V-JR1 en presencia de 8,8 μM BA. La adición de 0,5 a 5,3 mm de ácido indolacético o α-naftalenacético al medio, provocó el mayor porcentaje de formación de raíz en los brotes. Tanto la formación de brotes como la formación de raíz fue muy variable entre clones
Efecto de los filtrados de cultivos de Ophiostoma novo-ulmi Brasier sobre el crecimiento de callos de olmos con diferente susceptibilidad a la grafiosis
The long time needed to achieve results in traditional elm breeding programs against Dutch Elm Disease (DED) justifies the search for new selection techniques. In this paper, effects of Ophiostoma novo-ulmi culture filtrates on 20 elm genotypes were assayed, in an attempt to find out whether growth responses were related to their DED resistance level. Callus growth was lower with O. novo-ulmi culture filtrates. However, this response was independent of DED resistance. The influence of fungal media components, present in the control culture filtrates, on the callus hinders the use of these techniques in DED resistance selection.El largo tiempo de espera necesario para obtener resultados en los programas tradicionales de mejora de los olmos frente a la grafiosis justifica la búsqueda de nuevas técnicas de selección. En el presente trabajo se ha ensayado el efecto de los filtrados de cultivos de Ophiostoma novo-ulmi sobre los cultivos de tejidos de 20 genotipos de olmo, con la esperanza de obtener respuestas en su crecimiento relacionadas con su nivel de resistencia a la grafiosis. El crecimiento de los callos de olmo fue en general inferior en presencia de los filtrados de cultivos de O. novo-ulmi. Sin embargo, las respuestas de los callos de olmo a los excretados del hongo fueron independientes de su nivel de resistencia a la grafiosis. La influencia de los componentes del medio de cultivo del hongo, presentes en los filtrados de cultivos control, sobre las células de olmo dificulta la utilización de esta técnica en la selección de olmos resistentes a esta enfermedad
Automatic quantification of cardiomyocyte dimensions and connexin 43 lateralization in fluorescence images
Cardiomyocytes’ geometry and connexin 43 (CX43) amount and distribution are structural features that play a pivotal role in electrical conduction. Their quantitative assessment is of high interest in the study of arrhythmias, but it is usually hampered by the lack of automatic tools. In this work, we propose a software algorithm (Myocyte Automatic Retrieval and Tissue Analyzer, MARTA) to automatically detect myocytes from fluorescent microscopy images of cardiac tissue, measure their morphological features and evaluate the expression of CX43 and its degree of lateralization. The proposed software is based on the generation of cell masks, contouring of individual cells, enclosing of cells in minimum area rectangles and splitting of these rectangles into end-to-end and middle compartments to estimate CX43 lateral-to-total ratio. Application to human ventricular tissue images shows that mean differences between automatic and manual methods in terms of cardiomyocyte length and width are below 4 µm. The percentage of lateral CX43 also agrees between automatic and manual evaluation, with the interquartile range approximately covering from 3% to 30% in both cases. MARTA is not limited by fiber orientation and has an optimized speed by using contour filtering, which makes it run hundreds of times faster than a trained expert. Developed for CX43 studies in the left ventricle, MARTA is a flexible tool applicable to morphometric and lateralization studies of other markers in any heart chamber or even skeletal muscle. This open-access software is available online
Analysis of age-related left ventricular collagen remodeling in living donors: Implications in arrhythmogenesis
Age-related fibrosis in the left ventricle (LV) has been mainly studied in animals by assessing collagen content. Using second-harmonic generation microscopy and image processing, we evaluated amount, aggregation and spatial distribution of LV collagen in young to old pigs, and middle-age and elder living donors. All collagen features increased when comparing adult and old pigs with young ones, but not when comparing adult with old pigs or middle-age with elder individuals. Remarkably, all collagen parameters strongly correlated with lipofuscin, a biological age marker, in humans. By building patient-specific models of human ventricular tissue electrophysiology, we confirmed that amount and organization of fibrosis modulated arrhythmia vulnerability, and that distribution should be accounted for arrhythmia risk assessment. In conclusion, we characterize the age-associated changes in LV collagen and its potential implications for ventricular arrhythmia development. Consistency between pig and human results substantiate the pig as a relevant model of age-related LV collagen dynamics. © 2022 The Author(s
Search for New Physics with Jets and Missing Transverse Momentum in pp collisions at sqrt(s) = 7 TeV
A search for new physics is presented based on an event signature of at least
three jets accompanied by large missing transverse momentum, using a data
sample corresponding to an integrated luminosity of 36 inverse picobarns
collected in proton--proton collisions at sqrt(s)=7 TeV with the CMS detector
at the LHC. No excess of events is observed above the expected standard model
backgrounds, which are all estimated from the data. Exclusion limits are
presented for the constrained minimal supersymmetric extension of the standard
model. Cross section limits are also presented using simplified models with new
particles decaying to an undetected particle and one or two jets
Therapeutic implications of selecting the SCORE (European) versus the D'AGOSTINO (American) risk charts for cardiovascular risk assessment in hypertensive patients
Background: No comparisons have been made of scales estimating cardiovascular mortality and overall cardiovascular morbidity and mortality. The study objectives were to assess the agreement between the Framingham-D'Agostino cardiovascular risk (CVR) scale and the chart currently recommended in Europe (SCORE) with regard to identification of patients with high CVR, and to describe the discrepancies between them and the attendant implications for the treatment of hypertension and hyperlipidaemia. Methods: A total of 474 hypertensive patients aged 40-65 years monitored in primary care were enrolled into the study. CVR was assessed using the Framingham-D'Agostino scale, which estimates the overall cardiovascular morbidity and mortality risk, and the SCORE chart, which estimates the cardiovascular mortality risk. Cardiovascular risk was considered to be high for values ≥ 20% and ≥ 5% according to the Framingham-D'Agostino and SCORE charts respectively. Kappa statistics was estimated for agreement in classification of patients with high CVR. The therapeutic recommendations in the 2007 European Guidelines on Cardiovascular Disease Prevention were followed. Results
The Atapuerca sites and the Ibeas hominids
The Atapuerca railway Trench and Ibeas sites near Burgos, Spain,
are cave fillings that include a series of deposits ranging from
below the Matuyama/Bruhnes reversal up to the end of Middle
Pleistocene. The lowest fossil-bearing bed in the Trench contains
an assemblage of large and small Mammals including Mimomys
savini, Pitymys gregaloides, Pliomys episcopalis, Crocuta crocuta,
Dama sp. and Megacerini; the uppermost assemblage includes
Canis lupus, Lynx spelaea, Panthera (Leo) fossilis, Felis sylvestris,
Equus caballus steinheimensis, E.c. germanicus, Pitymys subtenaneus,
Microtus arvalis agrestis, Pliomys lenki, and also Panthera
toscana, Dicerorhinus bemitoechus, Bison schoetensacki, which are
equally present in the lowest level. The biostratigraphic correlation
and dates of the sites are briefly discussed, as are the
paleoclimatic interpretation of the Trench sequences. Stone artifacts
are found in several layers; the earliest occurrences correspond
to the upper beds containing Mimomys savini. A set of
preserved human occupation floors has been excavated in the top
fossil-bearing beds. The stone-tool assemblages of the upper levels
are of upper-medial Acheulean to Charentian tradition. The rich
bone breccia SH, in the Cueva Mayor-Cueva del Silo, Ibeas de
Juarros, is a derived deposit, due to a mud flow that dispersed and
carried the skeletons of many carnivores and humans. The taxa
represented are: Vrsus deningeri (largely dominant), Panthera (Leo)
fossilis, Vulpes vulpes, Homo sapiens var. Several traits of both
mandibular and cranial remains are summarized. Preliminary attempts
at dating suggest that the Ibeas fossil man is older than the
Last Interglacial, or oxygen-isotope stage 5
Associations between eating speed, diet quality, adiposity, and cardiometabolic risk factors
Objective: To assess the associations between eating speed, adiposity, cardiometabolic risk factors, and diet quality in a cohort of Spanish preschool-children. Study design: A cross-sectional study in 1371 preschool age children (49% girls; mean age, 4.8 ± 1.0 years) from the Childhood Obesity Risk Assessment Longitudinal Study (CORALS) cohort was conducted. After exclusions, 956 participants were included in the analyses. The eating speed was estimated by summing the total minutes used in each of the 3 main meals and then categorized into slow, moderate, or fast. Multiple linear and logistic regression models were fitted to assess the β-coefficient, or OR and 95% CI, between eating speed and body mass index, waist circumference, fat mass index (FMI), blood pressure, fasting plasma glucose, and lipid profile. Results: Compared with participants in the slow-eating category, those in the fast-eating category had a higher prevalence risk of overweight/obesity (OR, 2.9; 95% CI, 1.8-4.4; P < .01); larger waist circumference (β, 2.6 cm; 95% CI, 1.5-3.8 cm); and greater FMI (β, 0.3 kg/m2; 95% CI, 0.1-0.5 kg/m2), systolic blood pressure (β, 2.8 mmHg; 95% CI, 0.6-4.9 mmHg), and fasting plasma glucose levels (β, 2.7 mg/dL, 95% CI, 1.2-4.2 mg/dL) but lower adherence to the Mediterranean diet (β, −0.5 points; 95% CI, −0.9 to −0.1 points). Conclusions: Eating fast is associated with higher adiposity, certain cardiometabolic risk factors, and lower adherence to a Mediterranean diet. Further long-term and interventional studies are warranted to confirm these associations
Burden of community-acquired and nosocomial rotavirus gastroenteritis in the pediatric population of Western Europe: a scoping review
<p>Abstract</p> <p>Background</p> <p>Rotavirus affects 95% of children worldwide by age 5 years and is the leading cause of severe dehydrating diarrhea. The objective of this review was to estimate the burden of rotavirus gastroenteritis (RVGE) in the Western European pediatric population.</p> <p>Methods</p> <p>A comprehensive literature search (1999-2010) was conducted in PubMed and other sources (CDC; WHO, others). Data on the epidemiology and burden of RVGE among children < 5 years-old in Western Europe --including hospital-acquired disease--were extracted.</p> <p>Results</p> <p>76 studies from 16 countries were identified. The mean percentage of acute gastroenteritis (AGE) cases caused by rotavirus ranged from 25.3%-63.5% in children < 5 years of age, peaking during winter. Incidence rates of RVGE ranged from 1.33-4.96 cases/100 person- years. Hospitalization rates for RVGE ranged from 7% to 81% among infected children, depending on the country. Nosocomial RVGE accounted for 47%-69% of all hospital-acquired AGE and prolonged hospital stays by 4-12 days. Each year, RVGE incurred 53.6 million in direct medical costs and 22.4 million in indirect costs in the 16 countries studied. Full serotyping data was available for 8 countries. G1P[8], G2P[4], G9P[8], and G3P[8] were the most prevalent serotypes (cumulative frequency: 57.2%- 98.7%). Serotype distribution in nosocomial RVGE was similar.</p> <p>Conclusions</p> <p>This review confirms that RVGE is a common disease associated with significant morbidity and costs across Western Europe. A vaccine protecting against multiple serotypes may decrease the epidemiological and cost burden of RVGE in Western Europe.</p
Internet of Things for Environmental Sustainability and Climate Change
Our world is vulnerable to climate change risks such as glacier retreat, rising temperatures, more variable and intense weather events (e.g., floods, droughts, and frosts), deteriorating mountain ecosystems, soil degradation, and increasing water scarcity. However, there are big gaps in our understanding of changes in regional climate and how these changes will impact human and natural systems, making it difficult to anticipate, plan, and adapt to the coming changes. The IoT paradigm in this area can enhance our understanding of regional climate by using technology solutions, while providing the dynamic climate elements based on integrated environmental sensing and communications that is necessary to support climate change impacts assessments in each of the related areas (e.g., environmental quality and monitoring, sustainable energy, agricultural systems, cultural preservation, and sustainable mining). In the IoT in Environmental Sustainability and Climate Change chapter, a framework for informed creation, interpretation and use of climate change projections and for continued innovations in climate and environmental science driven by key societal and economic stakeholders is presented. In addition, the IoT cyberinfrastructure to support the development of continued innovations in climate and environmental science is discussed
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