Effect of 24-epibrassinolide on growth of in vitro shoot tips of different yam (Dioscorea Spp.) species

In this work we compared the effect of the growth regulator content of the culture medium on the growth of in vitro shoot tips of five yam accessions belonging to four yam species (one Dioscorea alata, one D. rotundata, one D. cay-enensis and two D. trifida). Medium S contained 0.6 μM benzyl adenine, 1.07 μM naphthalene acetic acid and 0.23 μM gibberellic acid while medium EBR contained 0.23 μM gibberellic acid and 0.1 μM 24-epibrassinolide. After 2 months of culture, oxidation level was significantly reduced on medium EBR compared to medium S for four of the five acces-sions tested. By contrast, medium EBR did not have any positive effect on shoot length since length of shoots produced after 2 months of culture on medium S and EBR were similar, except with accession 3-45T, for which shoot length was shorter on medium S compared to medium EBR. These results underline the potential of 24-epibrassinolide to reduce oxidation phenomena during in vitro culture and call for its utilization for regeneration of cryopreserved yam shoot tips, which is often impeded by oxidation phenomena

Multi-scale engineering for neuronal cell growth and differentiation

In this paper we investigate the role of micropatterning and molecular coating for cell culture and differentiation of neuronal cells (Neuro2a cell line) on a polydimethylsiloxane substrate. We investigate arrays of micrometric grooves (line and space) capable to guide neurite along their axis. We demonstrate that pattern dimensions play a major role due to the deformation of the cell occasioned by grooves narrower than typical cell dimension. A technological compromise for optimizing cell density, differentiation rate and neurite alignment has been obtained for 20 lm wide grooves which is a dimension comparable with the average cell dimension. This topographical engineered pattern combined with double-wall carbon nanotubes coating enabled us to obtain adherent cell densities in the order of 104 cells/cm2 and a differentiation rate close to 100%

Champs Conditionnels Aléatoires pour l'Annotation d'Arbres

National audienceAvec en vue la transformation de documents semi-structurés de type XML, nous nous intéressons au problème de l'annotation de tels documents par apprentissage statistique, à partir d'exemples de documents déjà annotés. Afin de modéliser la probabilité d'une annotation connaissant un document, nous nous plaçons dans le cadre des champs conditionnels aléatoires. Ce modèle a déjà fait ses preuves pour l'annotation de séquences : nous l'adaptons ici aux arbres ordonnés d'arité non bornée. Nous étudions l'expressivité du nouveau modèle ainsi introduit en le comparant aux automates d'arbres stochastiques (ou grammaires régulières probabilistes d'arbres). Nous présentons aussi en détail l'algorithme de recherche de l'annotation la plus probable et l'algorithme d'inférence pour ce modèle. Ces algorithmes sont implantés dans une librairie Tree CRF écrite en JAVA. Ces travaux sont des préliminaires qui nous permettront par la suite d'étudier les applications du modèle pour la transformation de documents

Quels liens entre climatologie, occupation des sols et inondations dans le bassin versant de l'Yzeron (ouest Lyonnais) ? Apport de l'analyse conjointe de données hydroclimatiques et d'images satellitaires très haute résolution

Le bassin versant de l'Yzeron, situé en périphérie immédiate de la ville de Lyon, est un bassin représentatif des bassins versants périurbains français. Il connaît, depuis les années 1970, une augmentation importante de son urbanisation. Ces dernières années, des crues importantes, entraînant des inondations à l'aval du bassin versant ont marqué les esprits, posant la question de l'impact des modifications de l'occupation des sols dans le bassin versant sur le régime des crues. Dans cet article, nous utilisons l'analyse combinée de données hydroclimatiques (pluie, température, évapotranspiration de référence -ET0, débits) et de cartes de l'occupation des sols dérivées d'images satellites SPOT à haute et très haute résolution pour aborder cette question. L'analyse des débits montre l'existence d'une évolution du régime des crues, avec une augmentation significative des crues sub -seuils pour des durées de 1,5h, 3h, 6h, 12h et 36h sur la période 1970-2010. L'analyse de l'échantillon de crues obtenue par cette analyse débit-durée-fréquence montre que les crues les plus importantes sont plutôt liées à des épisodes ayant conduit à un cumul de pluie important (> 80 mm en quelques jours). Une corrélation significative est aussi obtenue entre débit de base et coefficient de ruissellement, ce qui montre que ces crues peuvent être associées à des écoulements sur surfaces saturées. Les évolutions du climat (pluviométrie, température, ET0) ne permettent pas d'expliquer l'augmentation des crues observées. En revanche, l'occupation des sols entre 1990 et 2008 montre une augmentation des sur faces imperméables qui passent de 15,2 % de la surface totale du bassin en 1990 à 18,2 % en 1999 et 22,6 % en 2008. Cette évolution de l'imperméabilisation est mise en regard de l'accroissement de la population du bassin sur cette même période. Enfin, nous illustrons la réponse hydrologique du bassin versant avec deux épisodes pour lesquels le rôle de l'occupation des sols est différent. Le premier, celui du 02/12/2003 est typique d'une réponse par saturation du bassin, avec une contribution majeure des zon es rurales, suite à des cumuls pluviométriques importants. Le second, celui du 09/08/2009 représente un orage d'été, très court mais avec des intensités importantes, conduisant essentiellement à une réponse rapide des zones urbaines et pour lequel l'occupation des sols joue un rôle majeur

Conditional Random Fields for XML Applications

XML tree labeling is the problem of classifying elements in XML documents. It is a fundamental task for applications like XML transformation, schema matching, and information extraction. In this paper we propose XCRFs, conditional random fields for XML tree labeling. Dealing with trees often raises complexity problems. We describe optimization methods by means of constraints and combination techniques that allow XCRFs to be used in real tasks and in interactive machine learning programs. We show that domain knowledge in XML applications easily transfers in XCRFs thanks to constraints and combination of XCRFs. We describe an approach based on XCRF to learn tree transformations. The approach allows to solve xml data integration tasks and restructuration tasks. We have developed an open source toolbox for XCRFs. We use it to propose a Web service for the generation of personalized RSS feeds from HTML pages

Erlang-based dimensioning for IPv4 Address+Port translation

International audienceAs the IPv4 address pool is being exhausted, it becomes urgent to find a way to migrate IPv4 network architectures to IPv6, or to reduce the use of IPv4 addresses. In this paper, we discuss a strategy known as ''Address + Port'' translation, which consists in several users sharing the same IPv4 address and being distinguished by a range of port numbers. Of critical importance for the feasibility of such a mechanism is the knowledge of the minimum number of ports to allocate to users so that no service degradation is perceived. To that extent, we analyse the port consumption of the most port-consuming Internet applications, web browsing, and present some aggregate port consumption curves for the student population of our campus. Our results suggest that a port range of 1000 ports is totally transparent to users (which would allow to share a single IPv4 address among 64 users),while 400 ports (i.e., 150 users per address) is sufficient for most of users. Finally, the number of users per address could be further improved by benefiting from statistical multiplexing, i.e., using dynamical instead of fixed port range allocation

Effect of proline pretreatment on grapevine shoot-tip response to a droplet-vitrification protocol

Equipe DAVEM = Diversité et Adaptation de la Vigne et des Espèces MéditerranéennesProline has been shown to accumulate in plants in response to biotic and abiotic stresses. Exogenous proline has thus been used for improving some plant cryopreservation protocols. Further enhancement of cryopreservation efficiency for in vitro grapevines could be expected if stresses linked to cryopreservation procedures could be reduced. We therefore studied the possible beneficial effect of proline in grapevine cryopreservation. Single-node explants from in vitro grown grapevine plantlets (Vitis vinifera L. cv Portan) were cultured on shooting media (half-strength MS + 1 μM BAP) con- taining no proline (control) or 50, 500, or 2000 μM filter-sterilized L-proline. Shoot tips excised from these micro- shoots were subjected to a PVS2-based droplet-vitrification procedure. Control and rewarmed explants were grown on a recovery medium containing 1 μM BAP. Shoot development on control medium and lower proline concentrations did not notably differ whereas the highest concentration of proline inhibited shoot development. Carry-over effects were observed since lower survival and regrowth were obtained both for non-frozen or LN-treated explants excised from micro-shoots obtained on the 2000 μM proline medium. No significant differences in survival and regrowth were ob- served for non-frozen explants subjected to pretreatment without LN exposure. A slightly enhancing effect (although non-significant) on post-cryopreservation survival was observed for explants derived from shoots developed on 50 or 500 μM proline, but no significant improvement of regrowth percentage was observed for these two conditions. Al- though a slight increase in survival could be observed, no significant beneficial effect of proline pretreatment on post- cryoconservation regrowth could be evidenced in our conditions. However, the 2-week period before explant excision could have allowed at least partial metabolism and catabolism of exogenous proline; the results observed could thus have been the consequence of complex interactions. Shorter proline treatments applied closer to the actual LN exposure step might produce different results and allow for clearer interpretation

Ressources numériques en bibliothèque publique et scolaire: Etude pour la Suisse romande (Les)

C\u27est dans un contexte de dématérialisation des supports et d\u27une volonté pour les bibliothèques suisses de continuer à garantir à leurs usagers un accès libre et démocratique aux contenus que le comité romand de la Communauté de travail des bibliothèques suisses de lecture publique (CLP) a constitué un groupe de travail (GT). Celui-ci a été chargé de réfléchir à l’offre numérique des bibliothèques et en particulier à ce qui pourrait être mis en commun au niveau de la Suisse romande pour créer un portail d’accès à des ressources électroniques dédié aux bibliothèques publiques ou scolaires, quelle que soit leur taille. Ce rapport se veut avant tout un état des lieux des ressources offertes actuellement par les bibliothèques de lecture publique suisses permettant une réflexion pour le futur

Antinuclear Antibodies in Patients with Psoriatic Arthritis Treated or Not with Biologics

International audienceBackground:With the emergence of biotherapies, accurate diagnosis in early arthritis is needed. At this time, there is no biological marker of psoriatic arthritis.Objective:To test whether antinuclear antibodies (ANA) can be used as a diagnostic tool in psoriatic arthritis (PsA), we evaluated the prevalence of ANA in biologic-naïve PsA patients and in healthy blood donors.Methods:232 patients from the Rheumatology department, St Marguerite's Hospital, Marseilles, who fulfilled the CASPAR criteria for PsA, underwent clinical and laboratory investigations. Antinuclear antibodies (ANA), anti-extractable nuclear antigen antibodies (ENA), rheumatoid factor (RF), anti-citrullinated protein antibodies (ACPA) were assayed. Ninety-one healthy blood donors were also tested.Results:Detection of ANA by indirect immunofluorescence was significantly more frequent in sera from PsA patients than those from controls at serum dilution of 1:100 (57% compared with 40%, Odds Ratio (OR) 1.98 (1.2-3.4) p<0.02) and 1:160 (52% compared with 24%, OR 3,7 (1.9-7.2) p<0.001). No patients had lupus specific autoantibodies, 15 % had RF (34/232), and 1.7 % had ACPA (4/232).Conclusions:Detection of ANA was more frequent in sera from PsA patients than in those from healthy controls. This suggests that ANA could be a diagnosis orientation tool in PsA. Nevertheless, the specificity of these antibodies still remains to be investigated

SUMOylation promotes de novo targeting of HP1α to pericentric heterochromatin.

International audienceHP1 enrichment at pericentric heterochromatin is considered important for centromere function. Although HP1 binding to H3K9me3 can explain its accumulation at pericentric heterochromatin, how it is initially targeted there remains unclear. Here, in mouse cells, we reveal the presence of long nuclear noncoding transcripts corresponding to major satellite repeats at the periphery of pericentric heterochromatin. Furthermore, we find that major transcripts in the forward orientation specifically associate with SUMO-modified HP1 proteins. We identified this modification as SUMO-1 and mapped it in the hinge domain of HP1α. Notably, the hinge domain and its SUMOylation proved critical to promote the initial targeting of HP1α to pericentric domains using de novo localization assays, whereas they are dispensable for maintenance of HP1 domains. We propose that SUMO-HP1, through a specific association with major forward transcript, is guided at the pericentric heterochromatin domain to seed further HP1 localization