Vídeo-Clip de Animação

Neste relatório encontra-se o percurso da criação e produção do vídeo-clip “Back to Earth”, desde o argumento original por detrás da música ao estudo das personagens, passando pela realização, montagem, efeitos especiais, correção de cor e 3D (computação gráfica). Com o objetivo de realizar o argumento da animação, estudamos as tecnologias mais utilizadas, modernas e com melhores resultados. Assim, ao conhecermos estas tecnologias, podemos encontrar ou desenvolver a tecnologia para o vídeo-clip cujo objetivo principal seja a rapidez da criação 3D, a montagem, a coerência com o mundo real e, com isso, um baixo orçamento. Simultaneamente, o projeto proporcionou o aumento de competências que permitem percecionar as narrativas ou conceitos através dos caminhos supracitados, aumentando assim não só a sua compreensão, como ainda, a motivação do espetador.In this report we find the path of creation and production of the music video "Back to Earth", since the original argument behind the music to the study characters, through fulfillment, assembly, special effects, sound effects and 3D. Aiming to make the best argument of the animation, we study the 3D technologies commonly used, modern and with better results. Thus, by knowing these technologies, we may find or develop the technology for the film that the most important aspect has the rapid 3D creation, assembly, consistent with the real world and, thus, a low cost budget. Simultaneously, the project provided an increase of skills that allow perception narratives or concepts through the above paths, thereby increasing not only their understanding, but also the motivation of onlookers

LICITAÇÕES PÚBLICAS: ABRANGÊNCIA DAS SANÇÕES ADMINISTRATIVAS PREVISTAS NOS INCISOS III E IV DO ART. 87 DA LEI 8.666/93

Resumo: O presente artigo tem como objetivo abordar o entendimento dos doutrinadores em relação à real abrangência das sanções administrativas previstas nos incisos III e IV do art. 87 da lei 8.666/93. Através de pesquisa bibliográfica, buscou-se o entendimento de doutrinadores como Marçal Justen Filho, Maria S. Z. Di Pietro e Diogenes Gasparini a respeito do assunto, além de jurisprudências do Tribunal de Contas da União e Superior Tribunal de Justiça. Os resultados da pesquisa, desenvolvida por meio do método indutivo, denotam que ainda há divergência de entendimentos dos doutrinadores em relação da aplicação da sanção de suspensão temporária àquele que falha na execução de contratos com a Administração Pública. Mas que já há uma uniformização de entendimentos, por parte dos tribunais. Palavras-chave: Declaração de inidoneidade. Licitações públicas. Prerrogativas nos contratos. Sanções administrativas. Suspensão temporária

Servicios ecosistémicos en áreas de montaña: beneficios y amenazas

Mountain areas have a substantial impact on climate dynamics and are one of the most critical water sources. Mountains were key in human evolution throughout history and supplied essential biotic and abiotic ecosystem services (ES), key for human living. This perspective article will study the importance of mountains to ES supply and the impacts of the different drivers of change, namely habitat change, climate change, overexploitation, pollution, and invasive species. Mountain areas have a high capacity to supply an important number of regulating (global and local climate regulation, air quality regulation, natural hazards regulation, pollination), provisioning (crops, livestock, wild food and fish, biomass for energy and timber, freshwater renewable energy - hydropower, wind, solar and geothermal - and mineral resources) and cultural (recreation and tourism, landscape aesthetics and inspiration, cultural heritage and cultural diversity and knowledge systems). However, changes imposed by habitat change, climate change, overexploitation, pollution, and invasive species can increase the tradeoffs between ES and trigger environmental degradation. Overall, there is a need to balance mountain ES exploitation and reduce the effects of the different drivers of change.Las áreas de montaña tienen un impacto importante en las dinámicas climáticas y son una de las fuentes de agua con condiciones más críticas. Las montañas fueron claves en la evolución histórica del ser humano y suministraron servicios ecosistémicos (SE) bióticos y abióticos esenciales para la vida humana. Este artículo de perspectiva estudiará la importancia de las montañas para el suministro de SE y los impactos de los diferentes factores de cambio, a saber, cambios en el hábitat, el cambio climático, la sobreexplotación, la contaminación y las especies invasoras. Las zonas de montaña tienen una gran capacidad para suministrar un número importante de recursos reguladores (regulación del clima global y local, regulación de la calidad del aire, regulación de los riesgos naturales, polinización), de aprovisionamiento (cultivos, ganado, alimentos y pesca silvestre, biomasa para energía y madera, energía renovable de agua dulce -hidroeléctrica, eólica, solar y geotérmica- y recursos minerales) y culturales (ocio y turismo, estética e inspiración del paisaje, patrimonio cultural y diversidad cultural y sistemas de conocimiento). Sin embargo, los cambios impuestos por la modificación del hábitat, el cambio climático, la sobreexplotación, la contaminación y las especies invasoras pueden aumentar los intercambios entre los SE y desencadenar la degradación del medio ambiente. En general, es necesario equilibrar la utilización de los SE de montaña y reducir los efectos de los distintos factores de cambio

MicroRNA-mediated post-transcriptional regulation of Pinus pinaster response and resistance to pinewood nematode

Pine wilt disease (PWD), caused by the parasitic nematode Bursaphelenchus xylophilus, or pinewood nematode (PWN), is a serious threat to pine forests in Europe. Pinus pinaster is highly susceptible to the disease and it is currently the most affected European pine species. In this work, we investigated the role of small RNAs (sRNAs) in regulating P. pinaster–PWN interaction in an early stage of infection. After performing an artificial PWN inoculation assay, we have identified 105 plant microRNAs (miRNAs) responsive to PWN. Based on their predicted targets, part of these miRNAs was associated with roles in jasmonate-response pathway, ROS detoxification, and terpenoid biosynthesis. Furthermore, by comparing resistant and susceptible plants, eight miRNAs with putative functions in plant defence and resistance to PWN have been identified. Finally, we explored the possibility of bidirectional trans-kingdom RNA silencing, identifying several P. pinaster genes putatively targeted by PWN miRNAs, which was supported by degradome analysis. Targets for P. pinaster miRNAs were also predicted in PWN, suggesting a role for trans-kingdom miRNA transfer and gene silencing both in PWN parasitism as in P. pinaster resistance to PWD. Our results provide new insights into previously unexplored roles of sRNA post-transcriptional regulation in P. pinaster response and resistance to PWN.Fundação para a Ciência e a Tecnologia, I.P., the doctoral fellowship SFRH/BD/111687/2015, FCT/MCTES through national funds (PIDDAC) and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) of the EU, through Programa Operacional Regional de Lisboa do Portugal 2020.https://www.nature.com/srepBiochemistryGeneticsMicrobiology and Plant Patholog

SNP detection in Pinus pinaster transcriptome and association with resistance to pinewood nematode

Pinewood nematode (PWN, Bursaphelenchus xylophilus) is the causal agent of pine wilt disease (PWD), which severely affects Pinus pinaster stands in southwestern Europe. Despite the high susceptibility of P. pinaster, individuals of selected half-sib families have shown genetic variability in survival after PWN inoculation, indicating that breeding for resistance can be a valuable strategy to control PWD. In this work, RNA-seq data from susceptible and resistant plants inoculated with PWN were used for SNP discovery and analysis. A total of 186,506 SNPs were identified, of which 31 were highly differentiated between resistant and susceptible plants, including SNPs in genes involved in cell wall lignification, a process previously linked to PWN resistance. Fifteen of these SNPs were selected for validation through Sanger sequencing and 14 were validated. To evaluate SNP-phenotype associations, 40 half-sib plants were genotyped for six validated SNPs. Associations with phenotype after PWN inoculation were found for two SNPs in two different genes (MEE12 and PCMP-E91), as well as two haplotypes of HIPP41, although significance was not maintained following Bonferroni correction. SNPs here detected may be useful for the development of molecular markers for PWD resistance and should be further investigated in future association studies.SUPPLEMENTARY MATERIALS : Supplementary Figure S1. Type of SNPs identified in P. pinaster RNA-seq analysis; Supplementary Figure S2. Boxplots of the height and diameter at the base of the stem of inoculated plants (half-sib family 440) and t-test results for the comparison of these parameters’ means between susceptible and resistant plants; Supplementary Figure S3. Association analysis of the SNPs in the six sequenced gene fragments under different genetic models with resistance to PWN; Supplementary Figure S4. Genotypes distribution for SNPs associated with phenotype; Supplementary Table S1. Summary of PCR conditions and sequencing results of the 26 SNPs selected for validation; Supplementary Table S2. Summary of mapping statistic per sample and per sequencing lane; Supplementary Table S3. SNPs detected in P. pinaster RNA-seq data; Supplementary Table S4. Details and functional annotation of the SNPs with an Fst ≥ 0.80; Supplementary Table S5. Allele frequencies and Hardy–Weinberg Equilibrium significance values calculated by SNPassoc; Supplementary Table S6. Non-significant results of the haplotype association analysis obtained with SNPassoc. All analyses were performed using a logistics regression model.DATA AVAILABILITY STATEMENT : The sequencing data presented in this study are openly available in European Nucleotide Archive (ENA) at EMBL EBI under accession number PRJEB51636.This work was supported by Fundação para a Ciência e a Tecnologia, I.P. (FCT/MCTES), through Grants GREEN-it (UID/Multi/04551/2013), BioISI (UIDB/04046/2020 and UIDP/04046/2020) and the doctoral fellowship SFRH/BD/111687/2015 (to I.M.). Support was also provided by FCT/MCTES through national funds (PIDDAC) and co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) of the EU, through Programa Operacional Regional de Lisboa do Portugal 2020 or other programs that may succeed—PTDC/BAA-MOL/28379/2017, LISBOA-01-0145-FEDER-028379.https://www.mdpi.com/journal/forestsBiochemistryGeneticsMicrobiology and Plant Patholog

Discussões sobre corredores ecológicos no Brasil 2006-2016

Objetivou-se nesse estudo analisar as produções sobre o contexto de corredores ecológicos publicados em periódicos nacionais de Geografia no recorte temporal de 2006 a 2016, atentando para as discussões realizadas para conservação dos ecossistemas e de seus remanescentes. Para isso, a pesquisa foi desenvolvida mediante 4 fases de organização de dados: 1) Levantamento de Periódicos de Geografia de qualis A1, A2 e B1 na plataforma oficial de periódicos da CAPES (plataforma sucupira); 2) Levantamento nas edições das revistas, por artigos científicos que abordassem corredores ecológicos, 3) organização de quadro síntese quanto as variáveis título, área do conhecimento, nome da revista, objetivos, métodos e discussões dos trabalhos e 4) Exposição dos dados das produções de maneira quantitativa e qualitativa. Diante dessa fase de trabalho, objetiva-se futuramente propor um corredor ecológico no entorno do Parque Estadual do Desengano ao Parque estadual da Lagoa do Açu, visando conexão de ambientes e preservação

Assessing, quantifying and valuing the ecosystem services of coastal lagoons

The natural conservation of coastal lagoons is important not only for their ecological importance, but also because of the valuable ecosystem services they provide for human welfare and wellbeing. Coastal lagoons are shallow semi-enclosed systems that support important habitats such as wetlands, mangroves, salt-marshes and seagrass meadows, as well as a rich biodiversity. Coastal lagoons are also complex social-ecological systems with ecosystem services that provide livelihoods, wellbeing and welfare to humans. This study assessed, quantified and valued the ecosystem services of 32 coastal lagoons. The main findings of the study are: (i) the definitions of ecosystem services are still not generally accepted; (ii) the quantification of ecosystem services is made in many different ways, using different units; (iii) the evaluation in monetary terms of some ecosystem service is problematic, often relying on non-monetary evaluation methods; (iv) when ecosystem services are valued in monetary terms, this may represent very different human benefits; and, (v) different aspects of climate change, including increasing temperature, sea-level rise and changes in rainfall patterns threaten the valuable ecosystem services of coastal lagoons.DEVOTES project, from the European Union's Seventh Framework Programme for research, technological development and demonstration [308392]; networks and communities of Eurolag; Future Earth Coasts; SCOR; Fundacao para a Ciencia e a Tecnologia (FCT) Investigador Programme [IF/00331/2013]; Fundacao para a Ciencia e a Tecnologia [UID/MAR/04292/2013]; CESAM by FCT/MEC national funds (PIDDAC) [UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638]; FEDER; European Commission, under the 7th Framework Programme through the collaborative research project LAGOONS [283157]; FCT [SFRH/BPD/107823/2015, SFRH/BPD/91494/2012

Enrichment of B cell receptor signaling and epidermal growth factor receptor pathways in monoclonal gammopathy of undetermined significance: a genome-wide genetic interaction study

Background: Recent identification of 10 germline variants predisposing to monoclonal gammopathy of undetermined significance (MGUS) explicates genetic dependency of this asymptomatic precursor condition with multiple myeloma (MM). Yet much of genetic burden as well as functional links remain unexplained. We propose a workflow to expand the search for susceptibility loci with genome-wide interaction and for subsequent identification of genetic clusters and pathways. Methods: Polygenic interaction analysis on 243 cases/1285 controls identified 14 paired risk loci belonging to unique chromosomal bands which were then replicated in two independent sets (case only study, 82 individuals; case/control study 236 cases/ 2484 controls). Further investigation on gene-set enrichment, regulatory pathway and genetic network was carried out with stand-alone in silico tools separately for both interaction and genome-wide association study-detected risk loci. Results: Intronic-PREX1 (20q13.13), a reported locus predisposing to MM was confirmed to have contribution to excess MGUS risk in interaction with SETBP1, a well-established candidate predisposing to myeloid malignancies. Pathway enrichment showed B cell receptor signaling pathway (P < 5.3 × 10− 3) downstream to allograft rejection pathway (P < 5.6 × 10− 4) and autoimmune thyroid disease pathway (P < 9.3 × 10− 4) as well as epidermal growth factor receptor regulation pathway (P < 2.4 × 10− 2) to be differentially regulated. Oncogene ALK and CDH2 were also identified to be moderately interacting with rs10251201 and rs16966921, two previously reported risk loci for MGUS. Conclusions: We described novel pathways and variants potentially causal for MGUS. The methodology thus proposed to facilitate our search streamlines risk locus-based interaction, genetic network and pathway enrichment analyses