Ефектот на валпроат на кортикалната електрогенеза

Да покажеме некои наши клинички искуства за ефектот на хроничниот третман со валпрот на кортикалната електрогенеза, кое досега многу малку е студирано иако лекот е во употреба од 1964 година

COMMON NONCONFORMITIES DURING PROCEDURE FOR ACCREDITATION OF THE FOOD TESTING LABORATORIES IN THE R. MACEDONIA

During laboratory assessment in the frame of the accreditation procedure, they faced with number of nonconformities which are challenge for receiving the Certificate for accreditation and appropriate corrective measures shall be undertaken. Requirements that should be met by the food testing laboratories in the Republic of Macedonia, in order to gain accreditation certificate, contained in the standard МКС ENISO/IEC 17025:2006, ILAC (International Laboratory Accreditation Cooperation) document, ЕА (European Cooperation for Accreditation) document, Regulations and Procedures of the Institute for Accreditation of the Republic of Macedonia. In this paper the analysis is made and different proposals are given for the different ways of fulfilling of those requirements. The aim of this study is to emphasis the common nonconformities which are observed during realization of the Procedure of Accreditation of food testing laboratories and corrective measures undertaken. This investigation is of huge meaning for food testing laboratories which are already accredited and those which are planning to be accredited in the Republic of Macedonia. Furthermore, it is a very important for the Institute for accreditation of the Republic of Macedonia to come to conclusions for the weakest sides of the food testing laboratories and the assessment thereof. Such conclusions should initiate undertaking appropriate measures for improvement the Institute’s lead and technical assessors work towards nonconformities interpretation, identification and acceptance of the most suitable corrective measures

DIFFERENT APPROACHES IN ANALYZING CHYMOSIN PURITY

Chymosin is a specific proteolytic enzyme found in rennet, and is the key enzyme in cheese production classified in the aspartic endopeptidases (EC 3.4.23.4). The aim of this study was to determine the purity of different commercially available chymosins and its equivalents using electrophoretic and chromatographic techniques. Chymosins produced by the company Chr. Hansen, CHY-MAX 200 and CHY-MAX Plus, CHY-MAX PowderExtra NB, as well as Maxiren 1800 Granulate from the company DSM, Sirnik from SZR – Travnik, Kraljevo and Planika from Mikroprocessing, Bileca were used as materials for this study. The purity level of the commercially available enzymes was analyzed using electrophoretic (sodium dodecyl sulfate polyacrylamide gel electrophoresis or SDS-PAGE) and chromatographic (Rapid Resolution Liquid Chromatography or RRLC) techniques. Results showed no presence of undeclared protein fractions due to inappropriate purification process in the samples except for CHY-MAX М 200 which had two protein fractions, most likely as a result of a polymorphism. All the CHY-MAX and Maxiren samples have chymosin as the active component (36 kDa), except for Planika and Sirnik which have a natural protease from R. miehei. Chromatographic analysis showed that beside the active component (chymosin), the preservative sodium benzoate was present in varying concentrations in all but CHY-MAX PowderExtra NB

A global analysis of Y-chromosomal haplotype diversity for 23 STR loci

In a worldwide collaborative effort, 19,630 Y-chromosomes were sampled from 129 different populations in 51 countries. These chromosomes were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) and using the PowerPlex Y23 System (PPY23, Promega Corporation, Madison, WI). Locus-specific allelic spectra of these markers were determined and a consistently high level of allelic diversity was observed. A considerable number of null, duplicate and off-ladder alleles were revealed. Standard single-locus and haplotype-based parameters were calculated and compared between subsets of Y-STR markers established for forensic casework. The PPY23 marker set provides substantially stronger discriminatory power than other available kits but at the same time reveals the same general patterns of population structure as other marker sets. A strong correlation was observed between the number of Y-STRs included in a marker set and some of the forensic parameters under study. Interestingly a weak but consistent trend toward smaller genetic distances resulting from larger numbers of markers became apparent.Peer reviewe

Evoked potentials in evaluation of solcoseryl (actihamly) effects in the treatment of cerebral atrophic processies

Twenty-five patients with cerebral atrophic processes, either local or diffuse, of various etiology proved by computerized tomography were investigated and treated

Имуногенетски истражувања кај пациенти со епилепсија

Последните десет години Клиниката за неврологија во соработка со Институтот за трансфузиологија, Институтот за клиничка биохемија и Институтот за биологија при Академијата за наука спроведоа имуногенетски истражувања кај пациенти со епилепсија

Somatosensory evoked potentials in patients with thalamic pain

The aim of study was to examine how to the Somatosensory evoked potentials are affected in patients with cerebrovascular thalamic lesions and to correlate the findings with sensory abnormalities

Somatosensory evoked potentials in deaferentation pain

The aim of study was to examine how to the Somatosensory evoked potentials ( SEPs) are affected in patients with cerebrovascular thalamic lesions and to correlate the findings with sensory abnormalities