Biodiversidad

La diversidad biológica es un patrimonio de la humanidad y su buen uso está conectado con la supervivencia del ser human

Hacia un sistema de información geográfica integrado para las costas y los mares de Europa

La Agencia Europea de Medio Ambiente (AEMA), en colaboración con la Comisión Europea y los Estados miembros de la Unión Europea se encuentra involucrada en un ambicioso proceso de integración de las diferentes bases de datos e información ambienta, con el fin de crear una herramienta capaz de gestionar la puesta en marcha y la aplicación de las diferentes políticas europeas que afectan a los medios costero y marino en Europa. El desarrollo de todas aquellas políticas que afectan a este ámbito, entre otras la Directiva INSPIRE, la implantación de SEIS y las Recomendaciones para la Gestión Integrada de las Zonas Costeras (GIZC), requieren un mayor conocimiento y seguimiento de la información ambiental y de aspectos socio-económicos para la creación de análisis integrados que aporten respuestas integradas y sostenibles a las necesidades que se planteen. El seguimiento de la información ambiental precisa dotarse de desarrollos tecnológicos y sistemas de información eficaces, algunos buenos ejemplos ya están en funcionamiento a nivel europeo, como la Red Europea de Datos de Observación Marina en Europa (EMODNET) o el Sistema de Información de las Aguas Europeas (WISE).The European Environment Agency (EEA) in collaboration with the European Commission and the Member States of the European Union is involved in an ambitious process of integrating the existing databases on environmental information, in order to create a tool capable to manage the different European policies implementation and application affecting the coastal and marine environment in Europe. The policy development affecting this environmental context, amongst other initiatives the Directive INSPIRE, the implementation of SEIS and the Recommendations on Integrated Coastal Zone Management (ICZM), require a higher knowledge and monitoring initiatives for the environmental information, as well as the socio-economic aspects which support the integrated analysis for integrated and sustainable responses to concrete needs. The environmental information monitoring requires efficient technological solutions and information systems, some of them are already operative at European level, such as the European Marine Observation and Data Network (EMODNET) and the Water Information System for Europe (WISE)

Iberoamerican Contribution for the Achievement of the Objectives of the Roadmap to Accelerate Marine Spatial Planning Processes Worldwide

Desde 1997, la COI-UNESCO ha ido desarrollando y aplicando los conceptos de gestión y planificación costera y marina como parte de su estrategia institucional. Las conclusiones de la primera conferencia internacional sobre planificación espacial marina (PEM) en 2006, propició la publicación de la primera guía paso a paso en apoyo de los Estados miembros de la COI-UNESCO, en el desarrollo de planes espaciales marinos. La COI-UNESCO y la Comisión Europea se comprometieron en 2017 a promover el desarrollo de la PEM a nivel mundial a través de una hoja de ruta (MSProadmap) abierta a todos los países del mundo. Los países Iberoamericanos son beneficiarios activos de esta hoja de ruta y de la Iniciativa MSPglobal que implementa acciones en sus casos pilotos en el Mediterráneo occidental y el Pacífico sudeste. El objetivo es apoyar el avance de los procesos de planificación nacionales y favoreciendo la consideración de aspectos transfronterizos en el intercambio y la cooperación institucional a nivel regional. Este artículo pone en contexto el trabajo conjunto, presente y futuro, de la COI-UNESCO y sus Estados miembros iberoamericanos, en línea con los compromisos y objetivos de la Agenda 2030 y del Decenio de los Océanos (2021-2030).Since 1997, IOC-UNESCO has been developing and applying the concepts of coastal and marine management and planning, as part of its institutional strategy. The conclusions of the first international conference on marine spatial planning (MSP) in 2006 led to the publication of the first step-by-step guide to support IOC-UNESCO’s Member States in the development of marine spatial plans. IOC-UNESCO and the European Commission committed themselves in 2017 to promote the development of MSP at global level through a roadmap (MSProadmap) open to all countries of the world. Ibero-American countries are active beneficiaries of this roadmap and the MSP-global Initiative, in its pilot cases in the Western Mediterranean and the Southeast Pacific. The objective is to support the implementation of actions to advance national planning processes considering transboundary aspects in favor of institutional exchange and cooperation at regional level. This article puts into context the present and future joint work of the IOC-UNESCO and its Ibero-American Member States, in line with the commitments and objectives of the Agenda 2030 and the Ocean Decade (2021-2030)

USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy.

The spindle assembly checkpoint (SAC) is an essential mechanism that ensures the accurate chromosome segregation during mitosis, thus preventing genomic instability. Deubiquitinases have emerged as key regulators of the SAC, mainly by determining the fate of proteins during cell cycle progression. Here, we identify USP49 deubiquitinase as a novel regulator of the spindle checkpoint. We show that loss of USP49 in different cancer cell lines impairs proliferation and increases aneuploidy. In addition, USP49-depleted cells overcome the arrest induced by the SAC in the presence of nocodazole. Finally, we report new binding partners of USP49, including ribophorin 1, USP44, and different centrins.We thank A.P. Ugalde, D. Rodríguez, J.G. Pérez-Silva, Y. Español and F. Rodríguez for helpful comments and advice. We also thank S.A. Miranda, D. Álvarez-Puente and C. Garabaya for excellent technical assistance, as well as the staff of the scientific core facilities from the University of Oviedo (Unidad de Ensayos Biotecnológicos y Biomédicos, Servicios Científico-Técnicos). This work was supported by the Ministerio de Ciencia e Innovación (SAF2017-87655-R, PDI2020-118394RB-100 and PGC2018- 097019-B-I00), “la Caixa” Banking Foundation (project code HR17-00247), and the European Research Council (742067, DeAge). The IUOPA is funded by the Asturian Government and Fundación Cajastur-Liberbank.S

NOA36 Protein Contains a Highly Conserved Nucleolar Localization Signal Capable of Directing Functional Proteins to the Nucleolus, in Mammalian Cells

NOA36/ZNF330 is an evolutionarily well-preserved protein present in the nucleolus and mitochondria of mammalian cells. We have previously reported that the pro-apoptotic activity of this protein is mediated by a characteristic cysteine-rich domain. We now demonstrate that the nucleolar localization of NOA36 is due to a highly-conserved nucleolar localization signal (NoLS) present in residues 1–33. This NoLS is a sequence containing three clusters of two or three basic amino acids. We fused the amino terminal of NOA36 to eGFP in order to characterize this putative NoLS. We show that a cluster of three lysine residues at positions 3 to 5 within this sequence is critical for the nucleolar localization. We also demonstrate that the sequence as found in human is capable of directing eGFP to the nucleolus in several mammal, fish and insect cells. Moreover, this NoLS is capable of specifically directing the cytosolic yeast enzyme polyphosphatase to the target of the nucleolus of HeLa cells, wherein its enzymatic activity was detected. This NoLS could therefore serve as a very useful tool as a nucleolar marker and for directing particular proteins to the nucleolus in distant animal species

Epistemologías del Sur: confluencias, sinergias y proyecciones epistémico-educativas

The present work is based on the antecedent that traditional science is reproduced through education, typical of the positivist paradigm, constituting a technical-instrumental interest translated into the control of the environment, founding a technoscience centered on efficiency and efficiency, which is presented as one of the causes of global problems, for example, environmental crises and destruction of social tissues. Nevertheless, a situated-critical paradigm with the interests of situated-emancipatory knowledge emerges today. This paradigm is constructed from the pluralist dialogue between southern epistemologies, typical of knowledge and communities denied by normal and hegemonic science. Our interest is to contribute to the visibility of the emergence of southern epistemologies, studying the main epistemic nodes of Francisco Varela, Humberto Maturana, Leonardo Boff and Boaventura de Sousa Santos. Under the hypothesis that these epistemic elements converge complementarily, they make synergy in the emergence of new properties and epistemic conditions that are projected towards the epistemic-educational sphere. In the intersection and dialogical analysis, the categories emerged: human being as care, construction of knowledge as situated induction, learning as transformation in the bond and curriculum as ecology of knowledge. These categories are defined by the complementarity between the epistemic elements and the consequences of their application in the epistemic-educational sphere are interpreted. It concludes with a synthetic outline of an epistemic-educational proposal and discusses the limits and projections of the present study.El presente trabajo parte del antecedente que la ciencia tradicional es reproducida a través de la educación, propia del paradigma positivista, constitutivo de un interés técnico instrumental traducido en el control del medio, fundante de una tecnociencia centrada en la eficiencia y la eficacia, lo que se presenta como una de las causas de las problemáticas globales, por ejemplo, crisis ambientales y destrucción de tejidos sociales. No obstante, emerge en la actualidad un paradigma situado-crítico con intereses de conocimiento situado-emancipatorio. Este paradigma es construido a partir del diálogo pluralista entre las epistemologías del sur, propias de los saberes y comunidades negados por la ciencia normal y hegemónica. Nuestro interés es contribuir a la visibilización de la emergencia de las epistemologías del sur, estudiando los principales nodos epistémicos de Francisco Varela, Humberto Maturana, Leonardo Boff y Boaventura de Sousa Santos. Bajo la hipótesis de que estos elementos epistémicos confluyen complementariamente, hacen sinergia en la emergencia de nuevas propiedades y condiciones epistémicas que se proyectan hacia el ámbito epistémico-educativo. En el cruce y análisis dialógico emergieron las categorías: ser humano como cuidado, construcción de conocimiento como enacción situada, aprendizaje como transformación en el vínculo y currículum como ecología de saberes. Se definen estas categorías mediante la complementariedad entre los elementos epistémicos y se interpretan las consecuencias de su aplicación en el ámbito epistémico-educativo. Se concluye con un esbozo sintético de una propuesta epistémico-educativa y se discuten los límites y proyecciones del presente estudio

Integration and dissemination of aquatic biodiversity and ecosystem services data for case studies focusing on ecosystem-based management

Aquatic ecosystems –from marine and coastal to freshwater– are rich in biodiversity and home to a diverse array of species and habitats, providing numerous economic and societal benefits to the European population. Many of these valuable ecosystems are at risk of being irreversibly damaged by human activities and pressures, including pollution, contamination, hydromorphological alterations, invasive species, overfishing and climate change. These pressures threaten the sustainability of these ecosystems, their provision of ecosystem services and ultimately human well-being. AQUACROSS (Knowledge, Assessment, and Management for AQUAtic Biodiversity and Ecosystem Services aCROSS EU policies – http://aquacross.eu) seeks to advance the application of ecosystem-based management for aquatic ecosystems in an effort to support the timely achievement of the EU 2020 Biodiversity Strategy and other international conservation targets. In this regard, AQUACROSS aims to develop and test an assessment framework through a series of cases studies which considers the full array of interactions within aquatic ecosystems, including human activities. Following the Horizon 2020 Open Research Data Pilot, AQUACROSS addresses the challenge of bringing together newly generated as well as existing data used in the framework of the case studies, while at the same time supporting the project partners in terms of data integration and harmonisation. Through a lightweight CKAN-based information platform, we aim on one hand to support project partners in terms of discovery and data access (in interoperable formats), while on the other hand we offer operational support to open up raw and processed data for use in other contexts and disseminating these data and results. The latter is facilitated by the capabilities of the CKAN software which includes on-the-fly analysis and visualisation tools and enables easy data access through external software and tools such as R, QGis and Phyton. During this presentation we will report on the main lessons learned during this data integration exercise and focus on selected case study examples

Association of High Serum Levels of Growth Factors with Good Outcome in Ischemic Stroke : a Multicenter Study

Altres ajuts: This project was partially supported by grants from Xunta de Galicia (Consellería Educación: GRC2014/027 and IN607A2018/3), Spanish Research Network on Cerebrovascular Diseases RETICS-INVICTUS PLUS (RD16/0019), and by the European Union FEDER program.The main objective of this research work was to study the association of serum levels of growth factors (GF) and SDF-1α with the functional outcome and reduction of lesion volume in ischemic stroke patients. In this multicenter study, 552 patients with non-lacunar stroke (male, 62.1%; mean age, 68.2 ± 11.4) were included within 24 h from symptom onset. The main outcome variable was good functional outcome (modified Rankin Scale [mRS] ≤ 2) at 12 months. Secondary outcome variable was infarct volume (in mL) after 6 ± 3 months. Serum levels of VEGF, Ang-1, G-CSF, BDNF, and SDF-1α were measured by ELISA at admission, 7 ± 1 days, at 3 ± 1 months, and 12 ± 3 months. Except for BDNF, all GF and SDF-1α serum levels showed a peak value at day 7 and remained elevated during the first 3 months (all p < 0.01). High serum levels at day 7 of VEGF (OR, 19.3), Ang-1 (OR, 14.7), G-CSF (OR, 9.6), and SDF-1α (OR, 28.5) were independently associated with good outcome at 12 months (all p < 0.0001). On the other hand, serum levels of VEGF (B, − 21.4), G-CSF (B, − 14.0), Ang-1 (B, − 13.3), and SDF-1α (B, − 44.6) measured at day 7 were independently associated with lesion volume at 6 months (p < 0.01). In summary, high serum levels of VEGF, Ang-1, G-CSF, and SDF-1α at day 7 and 3 months after ischemic stroke are associated with good functional outcome and smaller residual lesion at 1 year of follow-up

Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015

Los genes de resistencia a colistina mcr-3 y mcr-1 se detectaron en un aislado de Escherichia coli de heces de ganado en un matadero español en 2015. Las secuencias de ambos genes se hibridaron a la misma banda de plásmidos de aproximadamente 250 kb, aunque la resistencia a la colistina no fue movilizable . El aislado producía betalactamasas de espectro extendido y pertenecía al serotipo O9: H10 y al tipo de secuencia ST533. Aquí informamos un gen mcr-3 detectado en Europa después de informes anteriores de Asia y los Estados Unidos.Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.• Ministerio de Economía, Industria y Competitividad. Proyecto AGL2016- 74882-C3 • Ministerio de Agricultura y Pesca (España) y Comunidad Autónoma de Comunidad Autónoma de Madrid. Ayuda S2013 / ABI-2747 • Junta de Extremadura y Fondo Europeo de Desarrollo Regional. Ayuda GR15075 e IB16073 • Fundación para la Ciencia y la Tecnología (Portugal). Ayudas UID / MAR / 04292/2013 • Fundación Tatiana de Guzmán El Bueno (España). Beca doctoral para María del Rocío Iglesias Parro • Instituto Nacional de Agricultura y Alimentación. Investigación y Tecnología (INIA). Beca doctoral para María del Rocío Iglesias Parro • Ministerio de Economía, Industria y Competitividad. Beca FPI2014-020, para Narciso Martín QuijadapeerReviewe

Estado Actual de las Colecciones de Microorganismos relacionadas a Sanidad Vegetal presentes en el INTA

PosterEn la campaña 2019/2020 la superficie cultivada fue superior a los 40,1 millones de ha, siendo los principales cultivos soja, maíz, trigo, girasol, sorgo, cebada, avena y centeno. Se destacan por su importancia regional algodón, poroto, maní y arroz. (Informe de Coyuntura Agrícola, Junio 2020, MDPA). Los cultivos de forrajeras ocupan 8 millones de ha, siendo la alfalfa la principal especie cultivada. La fruticultura ocupa 1,4 millones de ha y los cultivos hortícolas ocupan cerca de 700 mil ha. En el Instituto Nacional de Tecnología Agropecuaria(INTA) se ha generado una Red de RRGG y dentro de esta, una destinada a los Recursos Microbianos con la finalidad de compatibilizar protocolos de preservación, documentación, intercambio y adecuación de permisos de acceso vinculados al Protocolo de Nagoya.Instituto de Patología VegetalFil: Asselborn, Miriam. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Caligiore Gei, Pablo Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Campos, Pablo Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bordenave; ArgentinaFil: Colombo, Denis Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Colombo, Denis Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Comerio, Ricardo Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conforto, Erica Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; ArgentinaFil: Lucca, Ana Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Pedraza, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Valdez, Jorge Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Perticari, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Luis. Agencia De Extensión Rural Concarán; Argentin