1,112 research outputs found
The unreasonable effectiveness of equilibrium-like theory for interpreting non-equilibrium experiments
There has been great interest in applying the results of statistical
mechanics to single molecule experiements. Recent work has highlighted
so-called non-equilibrium work-energy relations and Fluctuation Theorems which
take on an equilibrium-like (time independent) form. Here I give a very simple
heuristic example where an equilibrium result (the barometric law for colloidal
particles) arises from theory describing the {\em thermodynamically}
non-equilibrium phenomenon of a single colloidal particle falling through
solution due to gravity. This simple result arises from the fact that the
particle, even while falling, is in {\em mechanical} equilibrium (gravitational
force equal the viscous drag force) at every instant. The results are
generalized by appeal to the central limit theorem. The resulting time
independent equations that hold for thermodynamically non-equilibrium (and even
non-stationary) processes offer great possibilities for rapid determination of
thermodynamic parameters from single molecule experiments.Comment: 6 page
Plastid osmotic stress influences cell differentiation at the plant shoot apex
The balance between proliferation and differentiation in the plant shoot apical meristem is controlled by regulatory loops involving the phytohormone cytokinin and stem cell identity genes. Concurrently, cellular differentiation in the developing shoot is coordinated with the environmental and developmental status of plastids within those cells. Here, we employ an Arabidopsis thaliana mutant exhibiting constitutive plastid osmotic stress to investigate the molecular and genetic pathways connecting plastid osmotic stress with cell differentiation at the shoot apex. msl2 msl3 mutants exhibit dramatically enlarged and deformed plastids in the shoot apical meristem, and develop a mass of callus tissue at the shoot apex. Callus production in this mutant requires the cytokinin receptor AHK2 and is characterized by increased cytokinin levels, downregulation of cytokinin signaling inhibitors ARR7 and ARR15, and induction of the stem cell identity gene WUSCHEL. Furthermore, plastid stress-induced apical callus production requires elevated plastidic reactive oxygen species, ABA biosynthesis, the retrograde signaling protein GUN1, and ABI4. These results are consistent with a model wherein the cytokinin/WUS pathway and retrograde signaling control cell differentiation at the shoot apex
Swimming in circles: Motion of bacteria near solid boundaries
Near a solid boundary, E. coli swims in clockwise circular motion. We provide
a hydrodynamic model for this behavior. We show that circular trajectories are
natural consequences of force-free and torque-free swimming, and the
hydrodynamic interactions with the boundary, which also leads to a hydrodynamic
trapping of the cells close to the surface. We compare the results of the model
with experimental data and obtain reasonable agreement. In particular, we show
that the radius of curvature of the trajectory increases with the length of the
bacterium body.Comment: Also available at http://people.deas.harvard.edu/~lauga
Hydrodynamic attraction of swimming microorganisms by surfaces
Cells swimming in confined environments are attracted by surfaces. We measure
the steady-state distribution of smooth-swimming bacteria (Escherichia coli)
between two glass plates. In agreement with earlier studies, we find a strong
increase of the cell concentration at the boundaries. We demonstrate
theoretically that hydrodynamic interactions of the swimming cells with solid
surfaces lead to their re-orientation in the direction parallel to the
surfaces, as well as their attraction by the closest wall. A model is derived
for the steady-state distribution of swimming cells, which compares favorably
with our measurements. We exploit our data to estimate the flagellar propulsive
force in swimming E. coli
Novel ultrastructures of Treponema primitia and their implications for motility
Members of the bacterial phylum Spirochaetes are generally helical cells propelled by periplasmic flagella. The spirochete Treponema primitia is interesting because of its mutualistic role in the termite gut, where it is believed to cooperate with protozoa that break down cellulose and produce H2 as a by-product. Here we report the ultrastructure of T. primitia as obtained by electron cryotomography of intact, frozen-hydrated cells. Several previously unrecognized external structures were revealed, including bowl-like objects decorating the outer membrane, arcades of hook-shaped proteins winding along the exterior and tufts of fibrils extending from the cell tips. Inside the periplasm, cone-like structures were found at each pole. Instead of the single peptidoglycan layer typical of other Gram-negative bacteria, two distinct periplasmic layers were observed. These layers formed a central open space that contained two flagella situated adjacent to each other. In some areas, the inner membrane formed flattened invaginations that protruded into the cytoplasm. High-speed light microscopic images of swimming T. primitia cells showed that cell bodies remained rigid and moved in a helical rather than planar motion. Together, these findings support the 'rolling cylinder' model for T. primitia motility that posits rotation of the protoplasmic cylinder within the outer sheath
Walks of molecular motors in two and three dimensions
Molecular motors interacting with cytoskeletal filaments undergo peculiar
random walks consisting of alternating sequences of directed movements along
the filaments and diffusive motion in the surrounding solution. An ensemble of
motors is studied which interacts with a single filament in two and three
dimensions. The time evolution of the probability distribution for the bound
and unbound motors is determined analytically. The diffusion of the motors is
strongly enhanced parallel to the filament. The analytical expressions are in
excellent agreement with the results of Monte Carlo simulations.Comment: 7 pages, 2 figures, to be published in Europhys. Let
Biomechanics for inclusive urban design : effects of tactile paving on older adults’ gait when crossing the street
In light of our ageing population it is important that the urban environment is easily accessible and hence supports older adults’ independence. Tactile ‘blister’ paving was originally designed to provide guidance for visually impaired people at pedestrian crossings. However, as research links irregular surfaces to falls in older adults, such paving may have an adverse effect on older people. We investigated the effects of tactile paving on older adults’ gait in a scenario closely resembling “crossing the street”. Gait analysis of 32 healthy older adults showed that tactile, as compared to smooth, paving increases the variability in timing of foot placement by 20%, thereby indicating a disturbance of the rhythmic gait pattern. Moreover, toe-clearance during the swing phase increased by 7% on tactile paving, and the ability to stop upon cue from the traffic light was compromised. These results need to be viewed under consideration of the limitations associated with laboratory studies and real world analysis is needed to fully understand their implications for urban design
Reproductive Toxicity and Life History Study of Silver Nanoparticle Effect, Uptake and Transport in Arabidopsis thaliana
Concerns about nanotechnology have prompted studies on how the release of these engineered nanoparticles impact our environment. Herein, the impact of 20 nm silver nanoparticles (AgNPs) on the life history traits of Arabidopsis thaliana was studied in both above- and below-ground parts, at macroscopic and microscopic scales. Both gross phenotypes (in contrast to microscopic phenotypes) and routes of transport and accumulation were investigated from roots to shoots. Wild type Arabidopsis growing in soil, regularly irrigated with 75 μg/L of AgNPs, did not show any obvious morphological change. However, their vegetative development was prolonged by two to three days and their reproductive growth shortened by three to four days. In addition, the germination rates of offspring decreased drastically over three generations. These findings confirmed that AgNPs induce abiotic stress and cause reproductive toxicity in Arabidopsis. To trace transport of AgNPs, this study also included an Arabidopsis reporter line genetically transformed with a green fluorescent protein and grown in an optical transparent medium with 75 μg/L AgNPs. AgNPs followed three routes: (1) At seven days after planting (DAP) at S1.0 (stages defined by Boyes et al. 2001 [41]), AgNPs attached to the surface of primary roots and then entered their root tips; (2) At 14 DAP at S1.04, as primary roots grew longer, AgNPs gradually moved into roots and entered new lateral root primordia and root hairs; (3) At 17 DAP at S1.06 when the Arabidopsis root system had developed multiple lateral roots, AgNPs were present in vascular tissue and throughout the whole plant from root to shoot. In some cases, if cotyledons of the Arabidopsis seedlings were immersed in melted transparent medium, then AgNPs were taken up by and accumulated in stomatal guard cells. These findings in Arabidopsis are the first to document specific routes and rates of AgNP uptake in vivo and in situ
Nematode-Induced Changes of Transporter Gene Expression in \u3ci\u3eArabidopsis\u3c/i\u3e Roots
Root-knot plant-parasitic nematodes (Meloidogyne spp.) account for much of the damage inflicted to plants by nematodes. The feeding sites of these nematodes consist of “giant” cells, which have characteristics of transfer cells found in other parts of plants. Increased transport activity across the plasma membrane is a hallmark of transfer cells, and giant cells provide nutrition for nematodes; therefore, we initiated a study to identify the transport processes that contribute to the development and function of nematode- induced feeding sites. The study was conducted over a 4-week period, during which time the large changes in the development of giant cells were documented. The Arabidopsis ATH1 GeneChip was used to identify the many transporter genes that were regulated by nematode infestation. Expression of 50 transporter genes from 18 different gene families was significantly changed upon nematode infestation. Sixteen transporter genes were studied in more detail using real-time reverse-transcriptase polymerase chain reaction to determine transcript abundance in nematode- induced galls that contain giant cells and uninfested regions of the root. Certain genes were expressed primarily in galls whereas others were expressed primarily in the uninfested regions of the root, and a third group was expressed evenly throughout the root. Multiple transport processes are regulated and these may play important roles in nematode feeding-site establishment and maintenance
TID and SEE Response of an Advanced Samsung 4G NAND Flash Memory
Initial total ionizing dose (TID) and single event heavy ion test results are presented for an unhardened commercial flash memory, fabricated with 63 nm technology. Results are that the parts survive to a TID of nearly 200 krad (SiO2), with a tractable soft error rate of about 10(exp -l2) errors/bit-day, for the Adams Ten Percent Worst Case Environment
- …