187 research outputs found
NILC_USP: an improved hybrid system for sentiment analysis in Twitter messages.
This paper describes the NILC USP system that participated in SemEval-2014 Task 9: Sentiment Analysis in Twitter, a re-run of the SemEval 2013 task under the same name. Our system is an improved version of the system that participated in the 2013 task. This system adopts a hybrid classification process that uses three classification approaches: rule-based, lexiconbased and machine learning. We suggest a pipeline architecture that extracts the best characteristics from each classifier. In this work, we want to verify how\ud
this hybrid approach would improve with better classifiers. The improved system achieved an F-score of 65.39% in the Twitter message-level subtask for 2013 dataset (+ 9.08% of improvement) and 63.94% for 2014 dataset.FAPESPSAMSUN
Dioxin-like, non-dioxin like PCB and PCDD/F contamination in European eel (Anguilla anguilla) from the Loire estuarine continuum: spatial and biological variabilities
To characterize the eel contamination by dioxin-like (dl) and non dioxin-like (ndl) PCBs and PCDD/Fs, 62 eels from the Loire estuary (France) were analyzed. PCB contamination significantly increased from glass eel stage (3.7 ±1.9 and 15.2±4.2 ng.g-1 dw) to other life stages (for yellow eels: 62.8±34.4 and 381.8±181.8 ng.g-1 dw; for silver eels: 93.7±56.3 and 463.2±244.6 ng.g-1 dw respectively for dl and ndl PCB). An inter-site variability based on PCB levels and fingerprints was observed between the three studied sites. The glass eel pattern was mainly characterized by the less chlorinated PCBs contrarily to the other eels, underlying a different bioaccumulation pathway. Overall, eels from this estuary showed an intermediate contamination level compared to other international/national areas. However, more than 60% of studied silver eels displayed WHO2005 PCDD/F and dl-PCB TEQ values higher than the recommended level of 10 pg.g-1 ww. This statement indicates a potential exposure to PCBs through eel consumption, especially with silver individuals, and could potentially lead to damages for the eel population
Maitotoxin-4, a Novel MTX Analog Produced by Gambierdiscus excentricus
Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area
NH2-terminal Deletion of ÎČ-Catenin Results in Stable Colocalization of Mutant ÎČ-Catenin with Adenomatous Polyposis Coli Protein and Altered MDCK Cell Adhesion
ÎČ-Catenin is essential for the function of cadherins, a family of Ca2+-dependent cellâcell adhesion molecules, by linking them to α-catenin and the actin cytoskeleton. ÎČ-Catenin also binds to adenomatous polyposis coli (APC) protein, a cytosolic protein that is the product of a tumor suppressor gene mutated in colorectal adenomas. We have expressed mutant ÎČ-catenins in MDCK epithelial cells to gain insights into the regulation of ÎČ-catenin distribution between cadherin and APC protein complexes and the functions of these complexes. Full-length ÎČ-catenin, ÎČ-catenin mutant proteins with NH2-terminal deletions before (ÎN90) or after (ÎN131, ÎN151) the α-catenin binding site, or a mutant ÎČ-catenin with a COOH-terminal deletion (ÎC) were expressed in MDCK cells under the control of the tetracycline-repressible transactivator. All ÎČ-catenin mutant proteins form complexes and colocalize with E-cadherin at cellâcell contacts; ÎN90, but neither ÎN131 nor ÎN151, bind α-catenin. However, ÎČ-catenin mutant proteins containing NH2-terminal deletions also colocalize prominently with APC protein in clusters at the tips of plasma membrane protrusions; in contrast, full-length and COOH-terminalâ deleted ÎČ-catenin poorly colocalize with APC protein. NH2-terminal deletions result in increased stability of ÎČ-catenin bound to APC protein and E-cadherin, compared with full-length ÎČ-catenin. At low density, MDCK cells expressing NH2-terminalâdeleted ÎČ-catenin mutants are dispersed, more fibroblastic in morphology, and less efficient in forming colonies than parental MDCK cells. These results show that the NH2 terminus, but not the COOH terminus of ÎČ-catenin, regulates the dynamics of ÎČ-catenin binding to APC protein and E-cadherin. Changes in ÎČ-catenin binding to cadherin or APC protein, and the ensuing effects on cell morphology and adhesion, are independent of ÎČ-catenin binding to α-catenin. These results demonstrate that regulation of ÎČ-catenin binding to E-cadherin and APC protein is important in controlling epithelial cell adhesion
α-Catenin-Vinculin Interaction Functions to Organize the Apical Junctional Complex in Epithelial Cells
αE-catenin, a cadherin-associated protein, is required for tight junction (TJ) organization, but its role is poorly understood. We transfected an αE-cateninâdeficient colon carcinoma line with a series of αE-catenin mutant constructs. The results showed that the amino acid 326â509 domain of this catenin was required to organize TJs, and its COOH-terminal domain was not essential for this process. The 326â509 internal domain was found to bind vinculin. When an NH2-terminal αE-catenin fragment, which is by itself unable to organize the TJ, was fused with the vinculin tail, this chimeric molecule could induce TJ assembly in the αE-cateninâdeficient cells. In vinculin-null F9 cells, their apical junctional organization was impaired, and this phenotype was rescued by reexpression of vinculin. These results indicate that the αE-catenin-vinculin interaction plays a role in the assembly of the apical junctional complex in epithelia
The secret life of α-catenin: Moonlighting in morphogenesis
Cadherin-based intercellular adhesions are important determinants of proper tissue architecture. These adhesions must be both stable and dynamic to maintain tissue integrity as cells undergo morphogenetic movements during development. The role of α-catenin in this process has been vigorously debated due to conflicting in vitro and in vivo evidence regarding its molecular mechanism of action. Recent data supports the classical view that α-catenin facilitates actin attachments at adherens junctions, but also suggests that α-catenin may act as a force transducer, and may have additional roles in the cytoplasm. These multiple functions for α-catenin converge on the regulation of adhesion and may help to explain its stable yet dynamic nature
Adenoma Formation following Limited Ablation of p120-Catenin in the Mouse Intestine
p120 loss destabilizes E-cadherin and could therefore result in tumor and/or metastasis-promoting activities similar to those caused by E-cadherin downregulation. Previously, we reported that p120 is essential in the intestine for barrier function, epithelial homeostasis and survival. Conditional p120 ablation in the mouse intestine induced severe inflammatory bowel disease, but long-term cancer-related studies were impossible because none of the animals survived longer than 21 days. Here, we used a tamoxifen-inducible mouse model (Vil-Cre-ERT2;p120fl/fl) to limit the extent of p120 ablation and thereby enable long-term studies. Reducing p120 KO to âŒ10% of the intestinal epithelium produced long-lived animals outwardly indistinguishable from controls. Effects of prolonged p120 absence were then evaluated at intervals spanning 2 to 18 months. At all time points, immunostaining revealed microdomains of p120-null epithelium interspersed with normal epithelium. Thus, stochastic p120 ablation is compatible with crypt progenitor cell function and permitted lifelong renewal of the p120-null cells. Consistent with previous observations, a barrier defect and frequent infiltration of neutrophils was observed, suggesting that focal p120 loss generates a microenvironment disposed to chronic inflammation. We report that 45% of these animals developed tumors within 18 months of tamoxifen induction. Interestingly, ÎČ-catenin was upregulated in the majority, but none of the tumors were p120 null. Although further work is required to directly establish mechanism, we conclude that limited p120 ablation can promote tumorigenesis by an indirect non-cell autonomous mechanism. Given that byproducts of inflammation are known to be highly mutagenic, we suggest that tumorigenesis in this model is ultimately driven by the lifelong inability to heal chronic wounds and the substantially increased rates of stochastic gene mutation in tissue microenvironments subjected to chronic inflammation. Indeed, although technical issues precluded direct identification of mutations, ÎČ-catenin upregulation in human colon cancer almost invariably reflects mutations in APC and/or ÎČ-catenin
Von der Kultivierung des Landes zur eigenstÀndigen Provinzkultur. Sieben Thesen zur Provinzarbeit
Das Land wird wieder vielschichtiger gesehen, nicht nur als Mangel- und Verlusterfahrung, sondern auch als Chance fĂŒr eine qualitativ andere Bildungsarbeit wahrgenommen. (DIPF/Orig.
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