20 research outputs found

    Hydrogen bonding of nitroxide spin labels in membrane proteins

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    On the basis of experiments at 275 GHz, we reconsider the dependence of the continuous-wave EPR spectra of nitroxide spin-labeled protein sites in sensory- and bacteriorhodopsin on the micro-environment. The high magnetic field provides the resolution necessary to disentangle the effects of hydrogen bonding and polarity. In the gxx region of the 275 GHz EPR spectrum, bands are resolved that derive from spin-label populations carrying no, one or two hydrogen bonds. The gxx value of each population varies hardly from site to site, significantly less than deduced previously from studies at lower microwave frequencies. The fractions of the populations vary strongly, which provides a consistent description of the variation of the average gxx and the average nitrogen-hyperfine interaction Azz from site to site. These variations reflect the difference in the proticity of the micro-environment, and differences in polarity contribute marginally. Concomitant W-band ELDOR- detected NMR experiments on the corresponding nitroxide in perdeuterated water resolve population-specific nitrogen-hyperfine bands, which underlies the interpretation for the proteins

    280 one-opposition near-Earth asteroids recovered by the EURONEAR with the <i>Isaac Newton</i> Telescope

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    Context. One-opposition near-Earth asteroids (NEAs) are growing in number, and they must be recovered to prevent loss and mismatch risk, and to improve their orbits, as they are likely to be too faint for detection in shallow surveys at future apparitions. Aims. We aimed to recover more than half of the one-opposition NEAs recommended for observations by the Minor Planet Center (MPC) using the Isaac Newton Telescope (INT) in soft-override mode and some fractions of available D-nights. During about 130 h in total between 2013 and 2016, we targeted 368 NEAs, among which 56 potentially hazardous asteroids (PHAs), observing 437 INT Wide Field Camera (WFC) fields and recovering 280 NEAs (76% of all targets). Methods. Engaging a core team of about ten students and amateurs, we used the THELI, Astrometrica, and the Find_Orb software to identify all moving objects using the blink and track-and-stack method for the faintest targets and plotting the positional uncertainty ellipse from NEODyS. Results. Most targets and recovered objects had apparent magnitudes centered around V ~ 22.8 mag, with some becoming as faint as V ~ 24 mag. One hundred and three objects (representing 28% of all targets) were recovered by EURONEAR alone by Aug. 2017. Orbital arcs were prolonged typically from a few weeks to a few years; our oldest recoveries reach 16 years. The O−C residuals for our 1854 NEA astrometric positions show that most measurements cluster closely around the origin. In addition to the recovered NEAs, 22 000 positions of about 3500 known minor planets and another 10 000 observations of about 1500 unknown objects (mostly main-belt objects) were promptly reported to the MPC by our team. Four new NEAs were discovered serendipitously in the analyzed fields and were promptly secured with the INT and other telescopes, while two more NEAs were lost due to extremely fast motion and lack of rapid follow-up time. They increase the counting to nine NEAs discovered by the EURONEAR in 2014 and 2015. Conclusions. Targeted projects to recover one-opposition NEAs are efficient in override access, especially using at least two-meter class and preferably larger field telescopes located in good sites, which appear even more efficient than the existing surveys

    Gravitational lensing analysis of the Kilo-Degree Survey

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    The Kilo-Degree Survey (KiDS) is a multi-band imaging survey designed for cosmological studies from weak lensing and photometric redshifts. It uses the European Southern Observatory VLT Survey Telescope with its wide-field camera OmegaCAM. KiDS images are taken in four filters similar to the Sloan Digital Sky Survey ugri bands. The best seeing time is reserved for deep r-band observations. The median 5σ limiting AB magnitude is 24.9 and the median seeing is below 0.7 arcsec. Initial KiDS observations have concentrated on the Galaxy and Mass Assembly (GAMA) regions near the celestial equator, where extensive, highly complete redshift catalogues are available. A total of 109 survey tiles, 1 square degree each, form the basis of the first set of lensing analyses of halo properties of GAMA galaxies. Nine galaxies per square arcminute enter the lensing analysis, for an effective inverse shear variance of 69 arcmin−2. Accounting for the shape measurement weight, the median redshift of the sources is 0.53. KiDS data processing follows two parallel tracks, one optimized for weak lensing measurement and one for accurate matched-aperture photometry (for photometric redshifts). This technical paper describes the lensing and photometric redshift measurements (including a detailed description of the Gaussian aperture and photometry pipeline), summarizes the data quality and presents extensive tests for systematic errors that might affect the lensing analyses. We also provide first demonstrations of the suitability of the data for cosmological measurements, and describe our blinding procedure for preventing confirmation bias in the scientific analyses. The KiDS catalogues presented in this paper are released to the community through http://kids.strw.leidenuniv.nl

    KiDS-450: cosmological parameter constraints from tomographic weak gravitational lensing

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    We present cosmological parameter constraints from a tomographic weak gravitational lensing analysis of ∌450 deg2 of imaging data from the Kilo Degree Survey (KiDS). For a flat Λ cold dark matter (ΛCDM) cosmology with a prior on H0 that encompasses the most recent direct measurements, we find S8â‰ĄÏƒ8Ωm/0.3−−−−−−√=0.745±0.039⁠. This result is in good agreement with other low-redshift probes of large-scale structure, including recent cosmic shear results, along with pre-Planck cosmic microwave background constraints. A 2.3σ tension in S8 and ‘substantial discordance’ in the full parameter space is found with respect to the Planck 2015 results. We use shear measurements for nearly 15 million galaxies, determined with a new improved ‘self-calibrating’ version of lensFIT validated using an extensive suite of image simulations. Four-band ugri photometric redshifts are calibrated directly with deep spectroscopic surveys. The redshift calibration is confirmed using two independent techniques based on angular cross-correlations and the properties of the photometric redshift probability distributions. Our covariance matrix is determined using an analytical approach, verified numerically with large mock galaxy catalogues. We account for uncertainties in the modelling of intrinsic galaxy alignments and the impact of baryon feedback on the shape of the non-linear matter power spectrum, in addition to the small residual uncertainties in the shear and redshift calibration. The cosmology analysis was performed blind. Our high-level data products, including shear correlation functions, covariance matrices, redshift distributions, and Monte Carlo Markov chains are available at http://kids.strw.leidenuniv.nl

    KiDS-450: cosmological parameter constraints from tomographic weak gravitational lensing

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    We present cosmological parameter constraints from a tomographic weak gravitational lensing analysis of ∌450 deg2 of imaging data from the Kilo Degree Survey (KiDS). For a flat Λ cold dark matter (ΛCDM) cosmology with a prior on H0 that encompasses the most recent direct measurements, we find S8â‰ĄÏƒ8Ωm/0.3−−−−−−√=0.745±0.039⁠. This result is in good agreement with other low-redshift probes of large-scale structure, including recent cosmic shear results, along with pre-Planck cosmic microwave background constraints. A 2.3σ tension in S8 and ‘substantial discordance’ in the full parameter space is found with respect to the Planck 2015 results. We use shear measurements for nearly 15 million galaxies, determined with a new improved ‘self-calibrating’ version of lensFIT validated using an extensive suite of image simulations. Four-band ugri photometric redshifts are calibrated directly with deep spectroscopic surveys. The redshift calibration is confirmed using two independent techniques based on angular cross-correlations and the properties of the photometric redshift probability distributions. Our covariance matrix is determined using an analytical approach, verified numerically with large mock galaxy catalogues. We account for uncertainties in the modelling of intrinsic galaxy alignments and the impact of baryon feedback on the shape of the non-linear matter power spectrum, in addition to the small residual uncertainties in the shear and redshift calibration. The cosmology analysis was performed blind. Our high-level data products, including shear correlation functions, covariance matrices, redshift distributions, and Monte Carlo Markov chains are available at http://kids.strw.leidenuniv.nl

    CLMM: a LSST-DESC cluster weak lensing mass modeling library for cosmology

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    International audienceWe present the v1.0 release of CLMM, an open source python library for the estimation of the weak lensing masses of clusters of galaxies. CLMM is designed as a stand-alone toolkit of building blocks to enable end-to-end analysis pipeline validation for upcoming cluster cosmology analyses such as the ones that will be performed by the Vera C. Rubin Legacy Survey of Space and Time-Dark Energy Science Collaboration (LSST-DESC). Its purpose is to serve as a flexible, easy-to-install, and easy-to-use interface for both weak lensing simulators and observers and can be applied to real and mock data to study the systematics affecting weak lensing mass reconstruction. At the core of CLMM are routines to model the weak lensing shear signal given the underlying mass distribution of galaxy clusters and a set of data operations to prepare the corresponding data vectors. The theoretical predictions rely on existing software, used as backends in the code, that have been thoroughly tested and cross-checked. Combined theoretical predictions and data can be used to constrain the mass distribution of galaxy clusters as demonstrated in a suite of example Jupyter Notebooks shipped with the software and also available in the extensive online documentation
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