Elckerlyc goes mobile - Enabling natural interaction in mobile user interfaces

The fast growth of computational resources and speech technology available on mobile devices makes it possible to entertain users of these devices in having a natural dialogue with service systems. These systems are sometimes perceived as social agents and this can be supported by presenting them on the interface by means of an animated embodied conversational agent. To take the full advantage of the power of embodied conversational agents in service systems it is important to support real-time, online and responsive interaction with the system through the embodied conversational agent. The design of responsive animated conversational agents is a daunting task. Elckerlyc is a model-based platform for the specification and animation of synchronised multi-modal responsive animated agents. This paper presents a new light-weight PictureEngine that allows to run this platform in mobile applications. We describe the integration of the PictureEngine in the user interface of two different coaching applications and discuss the findings from user evaluations. We also conducted a study to evaluate an editing tool for the specification of the agent’s communicative behaviour. Twenty one participants had to specify the behaviour of an embodied conversational agent using the PictureEngine. We may conclude that this new lightweight back-end engine for the Elckerlyc platform makes it easier to build embodied conversational interfaces for mobile devices

Elckerlyc goes mobile - Enabling natural interaction in mobile user interfaces

The fast growth of computational resources and speech technology available on mobile devices makes it possible to entertain users of these devices in having a natural dialogue with service systems. These systems are sometimes perceived as social agents and this can be supported by presenting them on the interface by means of an animated embodied conversational agent. To take the full advantage of the power of embodied conversational agents in service systems it is important to support real-time, online and responsive interaction with the system through the embodied conversational agent. The design of responsive animated conversational agents is a daunting task. Elckerlyc is a model-based platform for the speci﬿cation and animation of synchronised multi-modal responsive animated agents. This paper presents a new light-weight PictureEngine that allows to run this platform in mobile applications. We describe the integration of the PictureEngine in the user interface of two different coaching applications and discuss the ﬿ndings from user evaluations. We also conducted a study to evaluate an editing tool for the speci﬿cation of the agent’s communicative behaviour. Twenty one participants had to specify the behaviour of an embodied conversational agent using the PictureEngine. We may conclude that this new lightweight back-end engine for the Elckerlyc platform makes it easier to build embodied conversational interfaces for mobile devices

Performance and application of an open source automated magnetic optical density meter for analyzing magnetotactic bacteria

We present a spectrophotometer (optical density meter) combined with electromagnets dedicated to the analysis of magnetotactic bacteria. We have ensured that our system, called MagOD, can be easily reproduced by providing the source of the 3D prints for the housing, electronic designs, circuit board layouts, and microcontroller software. We compare the performance of this novel system to existing adapted commercial spectrophotometers. In addition, we demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a function of their orientation with respect to the light path and their speed of reorientation after the field has been rotated by 90o. We continuously monitored the development of a culture of magnetotactic bacteria over a period of five days, and measured the development of their velocity distribution over a period of one hour. Even though this dedicated spectrophotometer is relatively simple to construct and cost-effective, a range of magnetic field-dependent parameters can be extracted from suspensions of magnetotactic bacteria. Therefore, this instrument will help the magnetotactic research community to understand and apply this intriguing micro-organism

An open-source automated magnetic optical density meter for analysis of suspensions of magnetic cells and particles

We present a spectrophotometer (optical density meter) combined with electromagnets dedicated to the analysis of suspensions of magnetotactic bacteria. The instrument can also be applied to suspensions of other magnetic cells and magnetic particles. We have ensured that our system, called MagOD, can be easily reproduced by providing the source of the 3D prints for the housing, electronic designs, circuit board layouts, and microcontroller software. We compare the performance of our system to existing adapted commercial spectrophotometers. In addition, we demonstrate its use by analyzing the absorbance of magnetotactic bacteria as a function of their orientation with respect to the light path and their speed of reorientation after the field has been rotated by 90°. We continuously monitored the development of a culture of magnetotactic bacteria over a period of 5 days and measured the development of their velocity distribution over a period of one hour. Even though this dedicated spectrophotometer is relatively simple to construct and cost-effective, a range of magnetic field-dependent parameters can be extracted from suspensions of magnetotactic bacteria. Therefore, this instrument will help the magnetotactic research community to understand and apply this intriguing micro-organism

Collagen-Like Proteins in Pathogenic E. coli Strains

The genome sequences of enterohaemorrhagic E. coli O157:H7 strains show multiple open-reading frames with collagen-like sequences that are absent from the common laboratory strain K-12. These putative collagens are included in prophages embedded in O157:H7 genomes. These prophages carry numerous genes related to strain virulence and have been shown to be inducible and capable of disseminating virulence factors by horizontal gene transfer. We have cloned two collagen-like proteins from E. coli O157:H7 into a laboratory strain and analysed the structure and conformation of the recombinant proteins and several of their constituting domains by a variety of spectroscopic, biophysical, and electron microscopy techniques. We show that these molecules exhibit many of the characteristics of vertebrate collagens, including trimer formation and the presence of a collagen triple helical domain. They also contain a C-terminal trimerization domain, and a trimeric α-helical coiled-coil domain with an unusual amino acid sequence almost completely lacking leucine, valine or isoleucine residues. Intriguingly, these molecules show high thermal stability, with the collagen domain being more stable than those of vertebrate fibrillar collagens, which are much longer and post-translationally modified. Under the electron microscope, collagen-like proteins from E. coli O157:H7 show a dumbbell shape, with two globular domains joined by a hinged stalk. This morphology is consistent with their likely role as trimeric phage side-tail proteins that participate in the attachment of phage particles to E. coli target cells, either directly or through assembly with other phage tail proteins. Thus, collagen-like proteins in enterohaemorrhagic E. coli genomes may have a direct role in the dissemination of virulence-related genes through infection of harmless strains by induced bacteriophages

Calibration of the CMS hadron calorimeters using proton-proton collision data at root s=13 TeV

Methods are presented for calibrating the hadron calorimeter system of theCMSetector at the LHC. The hadron calorimeters of the CMS experiment are sampling calorimeters of brass and scintillator, and are in the form of one central detector and two endcaps. These calorimeters cover pseudorapidities vertical bar eta vertical bar ee data. The energy scale of the outer calorimeters has been determined with test beam data and is confirmed through data with high transverse momentum jets. In this paper, we present the details of the calibration methods and accuracy.Peer reviewe

A Straightforward Stereoselective Synthesis of d- and l-5-Hydroxy-4-hydroxymethyl-2-cyclohexenylguanine

A novel and facile synthesis of 5-hydroxy-4-hydroxymethyl-2-cyclohexenylguanine 1 is described. The key steps involve a Diels−Alder reaction of ethyl (2E)-3-acetyloxy-2-propenoate 2 as dienophile with Danishefsky's diene 3 to build up the six-membered ring skeleton, a Fraser-Reid reductive rearrangement of the adduct using LiAlH4, and base-moiety introduction using a Mitsunobu reaction. Optically pure d- and l-1 were obtained via resolution of intermediate 7 with (R)-(−)-methylmandelic acid. The synthetic procedure toward racemic 1 consists of only five steps and has proven to be highly efficient toward the synthesis of cyclohexenyl nucleosides