726 research outputs found
Label-Free Analysis and Sorting of Microalgae and Cyanobacteria in Microdroplets by Intrinsic Chlorophyll Fluorescence for the Identification of Fast Growing Strains.
Microalgae and cyanobacteria are promising organisms for sustainable biofuel production, but several challenges remain to make this economically viable, including identification of optimized strains with high biomass productivity. Here we report on a novel methodology for the label-free screening and sorting of cyanobacteria and microalgae in a microdroplet platform. We show for the first time that chlorophyll fluorescence can be used to measure differences in biomass between populations of picoliter microdroplets containing different species of cyanobacteria, Synechocystis PCC 6803 and Synechococcus PCC 7002, which exhibit different growth dynamics in bulk culture. The potential and robustness of this label-free screening approach is further demonstrated by the screening and sorting of cells of the green alga Chlamydomonas reinhardtii encapsulated in droplets.Author R.J.B. was supported by a Doctoral Training Grant from the Engineering and Physical Sciences Research Council (EPSRC) of the UK (Reference EP/P505445/1), and author J.J.L. was in receipt of a studentship from the Biotechnology and Biological Sciences Research Council (BBSRC) of the U.K. as part of the Cambridge BBSRC-DTP Programme (Reference BB/J014540/1). Authors S.A.-C., C.A., and A.G.S. acknowledge funding from the EC within the FP7 DEMA project, Grant Agreement No. 309086, and authors Z.Y., C.A., and A.G.S. were in receipt of funding from BBSRC sLoLa Award Reference BB/L002957/1.This is the final version of the article. It first appeared from the American Chemical Society via https://doi.org/10.1021/acs.analchem.6b0236
Testing T Invariance in the Interaction of Slow Neutrons with Aligned Nuclei
The study of five-fold (P even, T odd) correlation in the interaction of slow
polarized neutrons with aligned nuclei is a possible way of testing the time
reversal invariance due to the expected enhancement of T violating effects in
compound resonances. Possible nuclear targets are discussed which can be
aligned both dynamically as well as by the "brute force" method at low
temperature. A statistical estimation is performed of the five-fold correlation
for low lying p wave compound resonances of the Sb, Sb and
I nuclei. It is shown that a significant improvement can be achieved
for the bound on the intensity of the fundamental parity conserving time
violating (PCTV) interaction.Comment: 22 pages, 5 figures, published versio
The 14C(n,g) cross section between 10 keV and 1 MeV
The neutron capture cross section of 14C is of relevance for several
nucleosynthesis scenarios such as inhomogeneous Big Bang models, neutron
induced CNO cycles, and neutrino driven wind models for the r process. The
14C(n,g) reaction is also important for the validation of the Coulomb
dissociation method, where the (n,g) cross section can be indirectly obtained
via the time-reversed process. So far, the example of 14C is the only case with
neutrons where both, direct measurement and indirect Coulomb dissociation, have
been applied. Unfortunately, the interpretation is obscured by discrepancies
between several experiments and theory. Therefore, we report on new direct
measurements of the 14C(n,g) reaction with neutron energies ranging from 20 to
800 keV
Identifying Challenging Job and Environmental Demands of Older Nurses Within the National Health Service
Objectives: To explore the existing theoretical contexts of the job and environmental demands of the nursing profession in the National Health Service (NHS) and to investigate how these job and environmental demands impact on the personal constructs of older nurses within the NHS.
Background: Nursing is the single most widely practiced profession in the healthcare sector in the United Kingdom. However, nurses contend with challenging job and environmental demands on a daily basis, which deplete them of personal constructs (or resources) required to stay in the profession.
Methods: A multilevel exploratory qualitative research design was employed. Ten managers were interviewed for the preliminary study, based on which the three characteristics of an age-friendly NHS workplace were established: health, retirement, and flexibility. Then an in-depth literature review revealed that the most adversely affected job within the NHS was the nursing profession. Finally, a focus group study was undertaken with six older nurses working in the NHS.
Results: The most compelling finding of this study is that older nurses would generally not want to stay on the job if they had to work in the ward area. The physical, cognitive, and sensory constructs of older nurses are negatively affected by the job and environmental demands of the ward areas.
Conclusions: Understanding how these job and environmental demands of the workplace affect an older nurse’s personal constructs may help support a better design of nurse work and the wards and help extend the working lives of older nurses in the NHS
Modeling bursts and heavy tails in human dynamics
Current models of human dynamics, used from risk assessment to
communications, assume that human actions are randomly distributed in time and
thus well approximated by Poisson processes. We provide direct evidence that
for five human activity patterns the timing of individual human actions follow
non-Poisson statistics, characterized by bursts of rapidly occurring events
separated by long periods of inactivity. We show that the bursty nature of
human behavior is a consequence of a decision based queuing process: when
individuals execute tasks based on some perceived priority, the timing of the
tasks will be heavy tailed, most tasks being rapidly executed, while a few
experiencing very long waiting times. We discuss two queueing models that
capture human activity. The first model assumes that there are no limitations
on the number of tasks an individual can hadle at any time, predicting that the
waiting time of the individual tasks follow a heavy tailed distribution with
exponent alpha=3/2. The second model imposes limitations on the queue length,
resulting in alpha=1. We provide empirical evidence supporting the relevance of
these two models to human activity patterns. Finally, we discuss possible
extension of the proposed queueing models and outline some future challenges in
exploring the statistical mechanisms of human dynamics.Comment: RevTex, 19 pages, 8 figure
New measurement of neutron capture resonances of 209Bi
The neutron capture cross section of Bi209 has been measured at the CERN n
TOF facility by employing the pulse-height-weighting technique. Improvements
over previous measurements are mainly because of an optimized detection system,
which led to a practically negligible neutron sensitivity. Additional
experimental sources of systematic error, such as the electronic threshold in
the detectors, summing of gamma-rays, internal electron conversion, and the
isomeric state in bismuth, have been taken into account. Gamma-ray absorption
effects inside the sample have been corrected by employing a nonpolynomial
weighting function. Because Bi209 is the last stable isotope in the reaction
path of the stellar s-process, the Maxwellian averaged capture cross section is
important for the recycling of the reaction flow by alpha-decays. In the
relevant stellar range of thermal energies between kT=5 and 8 keV our new
capture rate is about 16% higher than the presently accepted value used for
nucleosynthesis calculations. At this low temperature an important part of the
heavy Pb-Bi isotopes are supposed to be synthesized by the s-process in the He
shells of low mass, thermally pulsing asymptotic giant branch stars. With the
improved set of cross sections we obtain an s-process fraction of 19(3)% of the
solar bismuth abundance, resulting in an r-process residual of 81(3)%. The
present (n,gamma) cross-section measurement is also of relevance for the design
of accelerator driven systems based on a liquid metal Pb/Bi spallation target.Comment: 10 pages, 5figures, recently published in Phys. Rev.
Measurement of the neutron capture cross section of the s-only isotope 204Pb from 1 eV to 440 keV
The neutron capture cross section of 204Pb has been measured at the CERN
n_TOF installation with high resolution in the energy range from 1 eV to 440
keV. An R-matrix analysis of the resolved resonance region, between 1 eV and
100 keV, was carried out using the SAMMY code. In the interval between 100 keV
and 440 keV we report the average capture cross section. The background in the
entire neutron energy range could be reliably determined from the measurement
of a 208Pb sample. Other systematic effects in this measurement could be
investigated and precisely corrected by means of detailed Monte Carlo
simulations. We obtain a Maxwellian average capture cross section for 204Pb at
kT=30 keV of 79(3) mb, in agreement with previous experiments. However our
cross section at kT=5 keV is about 35% larger than the values reported so far.
The implications of the new cross section for the s-process abundance
contributions in the Pb/Bi region are discussed.Comment: 8 pages, 3 figures, article submitted to Phys. Rev.
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