Expressionsanalyse neuer putativer Onkogene und Tumorsuppressorgene in Mamakarzinomen und deren Auswirkung auf Zellen in Kultur nach ektopischer Expression von cDNA

In der vorliegenden Arbeit wurde die Expression von 15 ausgewählten putativen Tumorsuppressor- und fünf potentiellen Onkogenen am Mammakarzinom untersucht. Anhand der mit cDNA-Dot-Blot-Hybridisierung und Echtzeit-PCR gesammelten experimentellen Daten konnten als Kandidatengene für weitere Analysen die reguliert exprimierten Gene ITIH5, Alpha-B-Crystallin, PIGR, capG, 14-3-3 zeta und FABP5 bestimmt werden. Nach Klonierung dieser Gene wurden ihre Auswirkungen auf das lokale Wachstum, die Proliferationsgeschwindigkeit und die Invasivität von Mammakarzinomzelllinien analysiert. Für die veränderte Expression der Kandidatengene ITIH5, capG und FABP5 lässt sich ein Zussamenhang mit Entstehung und Progression von Mammakarzinomen vermuten. Die Kandidatengene Alpha-B-Crystallin und PIGR scheinen keine Rolle in der Tumorgenese des Mammakarzinoms zu spielen. Da für das Kandidatengen 14-3-3 zeta zu wenig über mögliche Interaktionspartner bekannt ist, kann bei diesem Gen keine Aussage zur Relevanz in der Tumorgenese gemacht werden

Distinct Mechanisms of IgM Antibody-Mediated Acquired von Willebrand Syndrome and Successful Treatment with Recombinant von Willebrand Factor in One Patient

Acquired von Willebrand Syndrome (AVWS) is a rare coagulation disorder which can be associated with IgM paraproteinaemia. Recently, recombinant von Willebrand factor (rVWF) has become available for the treatment of bleedings in patients with inherited von Willebrand disease, but experience in patients with AVWS is limited. We report on 2 patients with AVWS with underlying IgM paraproteinaemia with distinct underlying pathomechanisms. In 1 patient, the paraprotein built unspecific complexes with von Willebrand factor (VWF). In the other patient, we were able to detect an IgM antibody against VWF. Bleeding in this patient was successfully treated with rVWF. To our knowledge, this is the first report about the successful use of rVWF in a patient with AVWS with the detection of a VWF-specific antibody

The selection and definition of indicators in public health monitoring for the 65+ age group in Germany

Selecting relevant indicators is an important step in the development of public health monitoring for older people. Indicators can be used to combine information comprehensively from various data sources and enable recurring, comparable findings to be made about the health of older people. Indicators were systematically compiled from existing international monitoring systems. An indicator set on health in old age was developed using a multistage, structured consensus-based process together with an interdisciplinary panel of experts. The resulting 18 indicators were assigned to three health areas: (1) environmental factors, (2) activities and participation, and (3) personal factors. Data sources that can be used for the indicators are the health surveys within the framework of the Robert Koch Institute’s (RKI) health monitoring system, as well as surveys from other research institutes and official statistics. In the future, the indicator set is to be developed further and integrated into an overall approach that is geared towards health reporting and the monitoring of chronic diseases in all phases of life

Application of a functional model for the modernization of devices designed to eliminate emergency oil spills

The article describes the stages of creation and composition of the functional model, which can be used for the design of oil spill response devices. The principle of operation of the functional model given in the article and it's graphical scheme are show

Identification of regulatory variants associated with genetic susceptibility to meningococcal disease.

Non-coding genetic variants play an important role in driving susceptibility to complex diseases but their characterization remains challenging. Here, we employed a novel approach to interrogate the genetic risk of such polymorphisms in a more systematic way by targeting specific regulatory regions relevant for the phenotype studied. We applied this method to meningococcal disease susceptibility, using the DNA binding pattern of RELA - a NF-kB subunit, master regulator of the response to infection - under bacterial stimuli in nasopharyngeal epithelial cells. We designed a custom panel to cover these RELA binding sites and used it for targeted sequencing in cases and controls. Variant calling and association analysis were performed followed by validation of candidate polymorphisms by genotyping in three independent cohorts. We identified two new polymorphisms, rs4823231 and rs11913168, showing signs of association with meningococcal disease susceptibility. In addition, using our genomic data as well as publicly available resources, we found evidences for these SNPs to have potential regulatory effects on ATXN10 and LIF genes respectively. The variants and related candidate genes are relevant for infectious diseases and may have important contribution for meningococcal disease pathology. Finally, we described a novel genetic association approach that could be applied to other phenotypes

Developing a framework for a public healthmonitoring of the population aged 65 years and older. Results of the IMOA workshop on concepts and indicators

Main goal of the Improving Health Monitoring in Older Age (IMOA) project is to provide a framework for an indicator-based public health monitoring of the population aged 65 years and older. The workshop served as a forum to discuss and agree upon relevant concepts with gerontologists, nursing care and public health scientists

Public Health Monitoring 65+ in Deutschland – Auswahl und Definition von Indikatoren

Die Auswahl relevanter Indikatoren ist ein wichtiger Schritt in der Entwicklung eines Public-Health-Monitorings zur Gesundheit im höheren Lebensalter. In diesem können Informationen aus verschiedenen Datenquellen gezielt und systematisch zusammengeführt werden, um wiederkehrende und vergleichbare Aussagen zur gesundheitlichen Lage älterer Menschen zu ermöglichen. Basierend auf einer systematischen Zusammenstellung bestehender Indikatoren aus internationalen Monitoringsystemen wurde in einem mehrstufigen strukturierten Konsentierungsprozess gemeinsam mit einem interdisziplinär besetzten Gremium aus Expertinnen und Experten ein Indikatorensatz zur Gesundheit im Alter entwickelt. Die 18 Indikatoren lassen sich den drei Handlungsfeldern (1) Lebensweltfaktoren, (2) Teilhabe und Aktivität sowie (3) personenbezogene Faktoren zuordnen. Datenquellen, die zur Erhebung der Indikatoren genutzt werden können, sind die Surveys des Gesundheitsmonitorings am Robert Koch-Institut, Erhebungen anderer Forschungseinrichtungen sowie Daten der amtlichen Statistik. Perspektivisch soll der Indikatorensatz weiterentwickelt und in ein Gesamtkonzept zur Gesundheitsberichterstattung und zum Monitoring chronischer Krankheiten in allen Lebensphasen integriert werden.Peer Reviewe

High Area Capacity Lithium-Sulfur Full-cell Battery with Prelitiathed Silicon Nanowire-Carbon Anodes for Long Cycling Stability

We show full Li/S cells with the use of balanced and high capacity electrodes to address high powerelectro-mobile applications. The anode is made of an assembly comprising of silicon nanowires asactive material densely and conformally grown on a 3D carbon mesh as a light-weight current collector,offering extremely high areal capacity for reversible Li storage of up to 9 mAh/cm2. The dense growthis guaranteed by a versatile Au precursor developed for homogenous Au layer deposition on 3Dsubstrates. In contrast to metallic Li, the presented system exhibits superior characteristics as an anodein Li/S batteries such as safe operation, long cycle life and easy handling. These anodes are combinedwith high area density S/C composite cathodes into a Li/S full-cell with an ether- and lithium triflatebasedelectrolyte for high ionic conductivity. The result is a highly cyclable full-cell with an areal capacityof 2.3 mAh/cm2, a cyclability surpassing 450 cycles and capacity retention of 80% after 150 cycles(capacity loss <0.4% per cycle). A detailed physical and electrochemical investigation of the SiNWLi/S full-cell including in-operando synchrotron X-ray diffraction measurements reveals that the lowerdegradation is due to a lower self-reduction of polysulfides after continuous charging/discharging

High Area Capacity Lithium-Sulfur Full-cell Battery with Prelitiathed Silicon Nanowire-Carbon Anodes for Long Cycling Stability

We show full Li/S cells with the use of balanced and high capacity electrodes to address high power electro-mobile applications. The anode is made of an assembly comprising of silicon nanowires as active material densely and conformally grown on a 3D carbon mesh as a light-weight current collector, offering extremely high areal capacity for reversible Li storage of up to 9 mAh/cm(2). The dense growth is guaranteed by a versatile Au precursor developed for homogenous Au layer deposition on 3D substrates. In contrast to metallic Li, the presented system exhibits superior characteristics as an anode in Li/S batteries such as safe operation, long cycle life and easy handling. These anodes are combined with high area density S/C composite cathodes into a Li/S full-cell with an ether- and lithium triflate-based electrolyte for high ionic conductivity. The result is a highly cyclable full-cell with an areal capacity of 2.3 mAh/cm(2), a cyclability surpassing 450 cycles and capacity retention of 80% after 150 cycles (capacity loss <0.4% per cycle). A detailed physical and electrochemical investigation of the SiNW Li/S full-cell including in-operando synchrotron X-ray diffraction measurements reveals that the lower degradation is due to a lower self-reduction of polysulfides after continuous charging/discharging