Temporal Resolution In Preschool Children

The purpose of this study was to examine temporal resolution in normal hearing pre-school children. Word recognition was evaluated in quiet and in spectrally identical continuous and interrupted noise at signal-to-noise ratios (S/Ns) of 10, 0, and -10 dB. Sixteen children four to five years of age and eight adults participated. Performance decreased with decreasing S/N. At poorer S/Ns, participants demonstrated superior performance or a release from masking in the interrupted noise. Adults performed better than children, yet, the release from masking was equivalent. Collectively these findings are consistent with the notion that preschool children suffer from poorer processing efficiency rather than temporal resolution per se

The response of the upper ocean to solar heating. I: The mixed layer

The results of two earlier papers on convection in the mixed layer and on the solar heating profile are here introduced into a one-dimensional model in order to investigate the following consequences of the daily cycle of solar heating in the upper ocean: 1. the daytime convection depth becomes less than the turbocline depth; 2. the convective power supply to turbulence in the mixed layer is reduced; 3. the mixed layer below the convection layer becomes stably stratified; 4. the depth of the turbocline is reduced, leaving a diurnal thermocline between it and the top of the seasonal thermocline; 5. the heat content and potential energy of the diurnal and seasonal thermoclines are increased, slowing down the subsequent nocturnal descent of the turbocline. These diurnal changes are illustrated by integrating a one-dimensional model forced by the astronomical cycle of solar heating and seasonal variation of surface meteorology derived from Bunker's climatology. The model is integrated for 18 months to show the seasonal modulation of the diurnal cycle. Nocturnal convection plays a dominant role. The convection depth closely follows the thermal compensation depth during the day when they are less than the turbocline depth. Integrating the model with a 24-hour time step leads to large errors in the seasonal variation of mixed layer temperature and depth, and in the source term of isopycnic potential vorticity. The errors are reduced by using two time steps per day, one for the daytime when convection is quenched, the other for the night when it is active. A novel parametrization based on tuning the daily equivalent solar elevation to surface temperature further reduces the error. This parametrization is used to investigate the sensitivity of the seasonal cycles of mixed layer depth and temperature to: (1) seasonality in the surface fluxes; (2) systematic changes in the net annual solar heating; (3) random changes in the seasonal cycles of solar heating induced (i) monthly and (ii) daily. The sensitivity to uncertainty in seawater turbidity is investigated in the same way. The profile of isopycnic potential vorticity subducted into the thermocline depends on the vernal correlation of mixed layer depth and density, so gyre circulation is sensitive to solar heating in spring

The long and short of it: the temporal significance of wealth and income

In the literatures on the lived experience of poverty and richness temporal dimensions are underappreciated. Comparing qualitative interviews with those at opposite ends of the income and wealth distributions in the UK, we examine a temporal contrast: while “poor” participants experience money as flows of income which focus orientation to the present and constrain orientation to the future, “rich” participants experience money not only as flows of income, but also in the form of a stock of wealth which facilitates long-term orientations. Highlighting the enduring nature of wealth and the comparative short-termism of income, we argue that the way in which capital and income relates to individuals' orientations to the future is important for understanding how economic inequality is experienced. Put differently, the form which economic resources take matters for one's ability to plan and control the future. This insight contributes to our understanding of the experience of being economically advantaged or disadvantaged, with implications for (social) policy

A Screen for Genes Expressed in the Olfactory Organs of Drosophila melanogaster Identifies Genes Involved in Olfactory Behaviour

BACKGROUND: For insects the sense of smell and associated olfactory-driven behaviours are essential for survival. Insects detect odorants with families of olfactory receptor proteins that are very different to those of mammals, and there are likely to be other unique genes and genetic pathways involved in the function and development of the insect olfactory system. METHODOLOGY/PRINCIPAL FINDINGS: We have performed a genetic screen of a set of 505 Drosophila melanogaster gene trap insertion lines to identify novel genes expressed in the adult olfactory organs. We identified 16 lines with expression in the olfactory organs, many of which exhibited expression of the trapped genes in olfactory receptor neurons. Phenotypic analysis showed that six of the lines have decreased olfactory responses in a behavioural assay, and for one of these we showed that precise excision of the P element reverts the phenotype to wild type, confirming a role for the trapped gene in olfaction. To confirm the identity of the genes trapped in the lines we performed molecular analysis of some of the insertion sites. While for many lines the reported insertion sites were correct, we also demonstrated that for a number of lines the reported location of the element was incorrect, and in three lines there were in fact two pGT element insertions. CONCLUSIONS/SIGNIFICANCE: We identified 16 new genes expressed in the Drosophila olfactory organs, the majority in neurons, and for several of the gene trap lines demonstrated a defect in olfactory-driven behaviour. Further characterisation of these genes and their roles in olfactory system function and development will increase our understanding of how the insect olfactory system has evolved to perform the same essential function to that of mammals, but using very different molecular genetic mechanisms

How a Diverse Research Ecosystem Has Generated New Rehabilitation Technologies: Review of NIDILRR’s Rehabilitation Engineering Research Centers

Over 50 million United States citizens (1 in 6 people in the US) have a developmental, acquired, or degenerative disability. The average US citizen can expect to live 20% of his or her life with a disability. Rehabilitation technologies play a major role in improving the quality of life for people with a disability, yet widespread and highly challenging needs remain. Within the US, a major effort aimed at the creation and evaluation of rehabilitation technology has been the Rehabilitation Engineering Research Centers (RERCs) sponsored by the National Institute on Disability, Independent Living, and Rehabilitation Research. As envisioned at their conception by a panel of the National Academy of Science in 1970, these centers were intended to take a “total approach to rehabilitation”, combining medicine, engineering, and related science, to improve the quality of life of individuals with a disability. Here, we review the scope, achievements, and ongoing projects of an unbiased sample of 19 currently active or recently terminated RERCs. Specifically, for each center, we briefly explain the needs it targets, summarize key historical advances, identify emerging innovations, and consider future directions. Our assessment from this review is that the RERC program indeed involves a multidisciplinary approach, with 36 professional fields involved, although 70% of research and development staff are in engineering fields, 23% in clinical fields, and only 7% in basic science fields; significantly, 11% of the professional staff have a disability related to their research. We observe that the RERC program has substantially diversified the scope of its work since the 1970’s, addressing more types of disabilities using more technologies, and, in particular, often now focusing on information technologies. RERC work also now often views users as integrated into an interdependent society through technologies that both people with and without disabilities co-use (such as the internet, wireless communication, and architecture). In addition, RERC research has evolved to view users as able at improving outcomes through learning, exercise, and plasticity (rather than being static), which can be optimally timed. We provide examples of rehabilitation technology innovation produced by the RERCs that illustrate this increasingly diversifying scope and evolving perspective. We conclude by discussing growth opportunities and possible future directions of the RERC program

APOE genotype influences insulin resistance, apolipoprotein CII and CIII according to plasma fatty acid profile in the Metabolic Syndrome

Metabolic markers associated with the Metabolic Syndrome (MetS) may be affected by interactions between the APOE genotype and plasma fatty acids (FA). In this study, we explored FA-gene interactions between the missense APOE polymorphisms and FA status on metabolic markers in MetS. Plasma FA, blood pressure, insulin sensitivity and lipid concentrations were determined at baseline and following a 12-week randomized, controlled, parallel, dietary FA intervention in 442 adults with MetS (LIPGENE study). FA-APOE gene interactions at baseline and following change in plasma FA were assessed using adjusted general linear models. At baseline E4 carriers had higher plasma concentrations of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and apolipoprotein B (apo B) compared with E2 carriers; and higher TC, LDL-C and apo B compared with E3/E3. Whilst elevated plasma n-3 polyunsaturated FA (PUFA) was associated with a beneficially lower concentration of apo CIII in E2 carriers, a high proportion of plasma C16:0 was associated with insulin resistance in E4 carriers. Following FA intervention, a reduction in plasma long-chain n-3 PUFA was associated with a reduction in apo CII concentration in E2 carriers. Our novel data suggest that individuals with MetS may benefit from personalized dietary interventions based on APOE genotype

MACHO Alert 95-30 : First Real-Time Observation of Extended Source Effects in Gravitational Microlensing

We present analysis of MACHO Alert 95-30, a dramatic gravitational microlensing event towards the Galactic bulge whose peak magnification departs significantly from the standard point-source microlensing model. Alert 95-30 was observed in real-time by the Global Microlensing Alert Network (GMAN), which obtained densely sampled photometric and spectroscopic data throughout the event. We interpret the light-curve ``fine structure'' as indicating transit of the lens across the extended face of the source star. This signifies resolution of a star several kpc distant. We find a lens angular impact parameter theta_{min}/theta_{source} = 0.715 +/- 0.003. This information, along with the radius and distance of the source, provides an additional constraint on the lensing system. Spectroscopic and photometric data indicate the source is an M4 III star of radius 61 +/- 12 Rsun, located on the far side of the bulge at 9 kpc. We derive a lens angular velocity, relative to the source, of 21.5 +/- 4.9 km/s/kpc, where the error is dominated by uncertainty in the source radius. Likelihood analysis yields a median lens mass of 0.67{+2.53}{-0.46} Msun, located with 80% probability in the Galactic bulge at a distance of 6.93{+1.56}{-2.25} kpc. If the lens is a main-sequence star, we can include constraints on the lens luminosity. This modifies our estimates to M_lens = 0.53{+0.52}{-0.35} Msun and D_lens = 6.57{+0.99}{-2.25} kpc. Spectra taken during the event show that the absorption line equivalent widths of H alpha and the TiO bands near 6700 A vary, as predicted for microlensing of an extended source. This is most likely due to center-to-limb variation in the stellar spectral lines. These data demonstrate the feasibility of using microlensing limb crossings as a tool to probe stellar atmospheres directly.Comment: 32 pages including 6 tables, and 15 figures; Uses AASTeX 4.0; submitted to The Astrophysical Journa

Out of Sight but Not out of Mind: Alternative Means of Communication in Plants

Current knowledge suggests that the mechanisms by which plants communicate information take numerous forms. Previous studies have focussed their attention on communication via chemicals, contact and light; other methods of interaction between plants have remained speculative. In this study we tested the ability of young chilli plants to sense their neighbours and identify their relatives using alternative mechanism(s) to recognised plant communication pathways. We found that the presence of a neighbouring plant had a significant influence on seed germination even when all known sources of communication signals were blocked. Furthermore, despite the signalling restriction, seedlings allocated energy to their stem and root systems differently depending on the identity of the neighbour. These results provide clear experimental evidence for the existence of communication channels between plants beyond those that have been recognized and studied thus far

Migration of myogenic cells in the rat extensor digitorum longus muscle studied with a split autograft model

The ability of myogenic cells to migrate perpendicular to the long axis of freely autografted muscles was examined. Rat extensor digitorum longus muscles were divided, and one half was devitalized by repeated freezing in liquid nitrogen while the other half was kept viable in physiologic saline. The halves were reunited with sutures and grafted back into the original muscle bed. At intervals between 5 and 25 days the grafts were removed and examined histologically for the presence of myotubes within the devitalized region. Myotubes were first seen in the devitalized half 10 days postgrafting with the maximum number of myotubes observed after 12 to 15 days. These results indicate that myogenic cells are capable of migration perpendicular to the long axis of the muscle fibers in an autograft.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47687/1/441_2004_Article_BF00327748.pd

Limb proportions show developmental plasticity in response to embryo movement

Animals have evolved limb proportions adapted to different environments, but it is not yet clear to what extent these proportions are directly influenced by the environment during prenatal development. The developing skeleton experiences mechanical loading resulting from embryo movement. We tested the hypothesis that environmentally-induced changes in prenatal movement influence embryonic limb growth to alter proportions. We show that incubation temperature influences motility and limb bone growth in West African Dwarf crocodiles, producing altered limb proportions which may, influence post-hatching performance. Pharmacological immobilisation of embryonic chickens revealed that altered motility, independent of temperature, may underpin this growth regulation. Use of the chick also allowed us to merge histological, immunochemical and cell proliferation labelling studies to evaluate changes in growth plate organisation, and unbiased array profiling to identify specific cellular and transcriptional targets of embryo movement. This disclosed that movement alters limb proportions and regulates chondrocyte proliferation in only specific growth plates. This selective targeting is related to intrinsic mTOR (mechanistic target of rapamycin) pathway activity in individual growth plates. Our findings provide new insights into how environmental factors can be integrated to influence cellular activity in growing bones and ultimately gross limb morphology, to generate phenotypic variation during prenatal development