1,817 research outputs found
Transcriptional Regulation of zma-MIR528a by Action of Nitrate and Auxin in Maize
In recent years, miR528, a monocot-specific miRNA, has been assigned multifaceted roles during development and stress response in several plant species. However, the transcription regulation and the molecular mechanisms controlling MIR528 expression in maize are still poorly explored. Here we analyzed the zma-MIR528a promoter region and found conserved transcription factor binding sites related to diverse signaling pathways, including the nitrate (TGA1/4) and auxin (AuxRE) response networks. Accumulation of both pre-miR528a and mature miR528 was up-regulated by exogenous nitrate and auxin treatments during imbibition, germination, and maize seedling establishment. Functional promoter analyses demonstrated that TGA1/4 and AuxRE sites are required for transcriptional induction by both stimuli. Overall, our findings of the nitrogen- and auxin-induced zma-MIR528a expression through cis-regulatory elements in its promoter contribute to the knowledge of miR528 regulome
Search for blue compact dwarf galaxies during quiescence II: metallicities of gas and stars, ages, and star-formation rates
We examine the metallicity and age of a large set of SDSS/DR6 galaxies that
may be Blue Compact Dwarf (BCD) galaxies during quiescence (QBCDs).The
individual spectra are first classified and then averaged to reduce noise. The
metallicity inferred from emission lines (tracing ionized gas) exceeds by ~0.35
dex the metallicity inferred from absorption lines (tracing stars). Such a
small difference is significant according to our error budget estimate. The
same procedure was applied to a reference sample of BCDs, and in this case the
two metallicities agree, being also consistent with the stellar metallicity in
QBCDs. Chemical evolution models indicate that the gas metallicity of QBCDs is
too high to be representative of the galaxy as a whole, but it can represent a
small fraction of the galactic gas, self enriched by previous starbursts. The
luminosity weighted stellar age of QBCDs spans the whole range between 1 and 10
Gyr, whereas it is always smaller than 1 Gyr for BCDs. Our stellar ages and
metallicities rely on a single stellar population spectrum fitting procedure,
which we have specifically developed for this work using the stellar library
MILES.Comment: Accepted for publication in ApJ. 20 pages. 16 figures (corrected
typos
Staging Parkinson’s Disease Combining Motor and Nonmotor Symptoms Correlates with Disability and Quality of Life
Malaltia de Parkinson; Qualitat de vidaEnfermedad de Parkinson; Calidad de vidaParkinson's Disease; Quality of LifeIntroduction. In a degenerative disorder such as Parkinson’s disease (PD), it is important to establish clinical stages that allow to know the course of the disease. Our aim was to analyze whether a scale combining Hoehn and Yahr’s motor stage (H&Y) and the nonmotor symptoms burden (NMSB) (assessed by the nonmotor symptoms scale (NMSS)) provides information about the disability and the patient’s quality of life (QoL) with regard to a defined clinical stage. Materials and Methods. Cross-sectional study in which 603 PD patients from the COPPADIS cohort were classified according to H&Y (1, stage I; 2, stage II; 3, stage III; 4, stage IV/V) and NMSB (A: NMSS = 0–20; B: NMSS = 21–40; C: NMSS = 41–70; D: NMSS ≥ 71) in 16 stages (HY.NMSB, from 1A to 4D). QoL was assessed with the PDQ-39SI, PQ-10, and EUROHIS-QOL8 and disability with the Schwab&England ADL (Activities of Daily Living) scale. Results. A worse QoL and greater disability were observed at a higher stage of H&Y and NMSB (). Combining both (HY.NMSB), patients in stages 1C and 1D and 2C and 2D had significantly worse QoL and/or less autonomy for ADL than those in stages 2A and 2B and 3A and 3B, respectively (; e.g., PDQ-39SI in 1D [n = 15] vs 2A [n = 101]: 28.6 ± 17.1 vs 7.9 ± 5.8; ). Conclusion. The HY.NMSB scale is simple and reflects the degree of patient involvement more accurately than the H&Y. Patients with a lower H&Y stage may be more affected if they have a greater NMS burden
The stellar mass assembly of galaxies from z=0 to z=4. Analysis of a sample selected in the rest-frame near-infrared with Spitzer
Using a sample of ~28,000 sources selected at 3.6-4.5 microns with Spitzer
observations of the HDF-N, the CDF-S, and the Lockman Hole (surveyed area: ~664
arcmin^2), we study the evolution of the stellar mass content of the Universe
at 0<z<4. We calculate stellar masses and photometric redshifts, based on
~2,000 templates built with stellar and dust emission models fitting the
UV-to-MIR SEDs of galaxies with spectroscopic redshifts. We estimate stellar
mass functions for different redshift intervals. We find that 50% of the local
stellar mass density was assembled at 0<z<1 (average SFR:0.048 M_sun/yr/Mpc^3),
and at least another 40% at 1<z<4 (average SFR: 0.074 M_sun/yr/Mpc^3). Our
results confirm and quantify the ``downsizing'' scenario of galaxy formation.
The most massive galaxies (M>10^12.0 M_sun) assembled the bulk of their stellar
content rapidly (in 1-2 Gyr) beyond z~3 in very intense star formation events
(producing high specific SFRs). Galaxies with 10^11.5<M/M_sun<10^12.0 assembled
half of their stellar mass before z~1.5, and more than 90% of their mass was
already in place at z~0.6. Galaxies with M<10^11.5 M_sun evolved more slowly
(presenting smaller specific SFRs), assembling half of their stellar mass below
z~1. About 40% of the local stellar mass density of 10^9.0<M/M_sun<10^11.0
galaxies was assembled below z~0.4, most probably through accretion of small
satellites producing little star formation. The cosmic stellar mass density at
z>2.5 is dominated by optically faint (R>25) red galaxies (Distant Red Galaxies
or BzK sources) which account for ~30% of the global population of galaxies,
but contribute at least 60% to the cosmic stellar mass density. Bluer galaxies
(e.g., Lyman Break Galaxies) are more numerous but less massive, contributing
less than 50% to the global stellar mass density at high redshift.Comment: Published in ApJ. 38 pages, 10 figures, 5 tables, 2 appendices. Some
changes to match the final published versio
MicroRNA-223 is a novel negative regulator of HSP90B1 in CLL
This is an Open Access article distributed under the terms of the
Creative Commons Attribution License.-- et al.[Background]: MicroRNAs are known to inhibit gene expression by binding to the 3'UTR of the target transcript. Downregulation of miR-223 has been recently reported to have prognostic significance in CLL. However, there is no evidence of the pathogenetic mechanism of this miRNA in CLL patients. [Methods]: By applying next-generation sequencing techniques we have detected a common polymorphism (rs2307842), in 24% of CLL patients, which disrupts the binding site for miR-223 in HSP90B1 3'UTR. We investigated whether miR-223 directly targets HSP90B1 through luciferase assays and ectopic expression of miR-223. Quantitative real-time polymerase chain reaction and western blot were used to determine HSP90B1 expression in CLL patients. The relationship between rs2307842 status, HSP90B1 expression and clinico-biological data were assessed. [Results]: HSP90B1 is a direct target for miR-223 by interaction with the putative miR-223 binding site. The analysis in paired samples (CD19+ fraction cell and non-CD19+ fraction cell) showed that the presence of rs2307842 and IGHV unmutated genes determined HSP90B1 overexpression in B lymphocytes from CLL patients. These results were confirmed at the protein level by western blot. Of note, HSP90B1 overexpression was independently predictive of shorter time to the first therapy in CLL patients. By contrast, the presence of rs2307842 was not related to the outcome. [Conclusions]: HSP90B1 is a direct target gene of miR-223. Our results provide a plausible explanation of why CLL patients harboring miR-223 downregulation are associated with a poor outcome, pointing out HSP90B1 as a new pathogenic mechanism in CLL and a promising therapeutic target.This work was partially supported by grants from the Spanish Fondo de Investigaciones Sanitarias FIS 09/01543 and PI12/00281, Proyectos de Investigación del SACYL 355/A/09, COST Action EuGESMA (BM0801), Fundación Manuel Solórzano, Obra Social Banca Cívica (Caja Burgos), Fundación Española de Hematología y Hemoterapia (FEHH) and by a grant (RD12/0036/0069) from the Red Temática de Investigación Cooperativa en Cáncer (RTICC), Instituto de Salud Carlos III (ISCIII), Spanish Ministry of Economy and Competitiveness & European Regional Development Fund (ERDF) “Una manera de hacer Europa” (Innocampus). The research leading to these results has received funding from the European Union Seventh Framework Programme [FP7/2007-2013] under Grant Agreement n°306242-NGS-PTL. MHS is fully supported by an Ayuda predoctoral de la Junta de Castilla y Leon by the Fondo Social Europeo. ME Sarasquete is supported by Contrato Miguel Servet (CP13/00080).Peer Reviewe
Aperture effects on the oxygen abundance determinations from CALIFA data
This paper aims at providing aperture corrections for emission lines in a
sample of spiral galaxies from the Calar Alto Legacy Integral Field Area Survey
(CALIFA) database. In particular, we explore the behavior of the
log([OIII]5007/Hbeta)/([NII]6583/Halpha) (O3N2) and log[NII]6583/Halpha (N2)
flux ratios since they are closely connected to different empirical
calibrations of the oxygen abundances in star forming galaxies.
We compute median growth curves of Halpha, Halpha/Hbeta, O3N2 and N2 up to
2.5R_50 and 1.5 disk R_eff. The growth curves simulate the effect of observing
galaxies through apertures of varying radii. The median growth curve of the
Halpha/Hbeta ratio monotonically decreases from the center towards larger
radii, showing for small apertures a maximum value of ~10% larger than the
integrated one. The median growth curve of N2 shows a similar behavior,
decreasing from the center towards larger radii. No strong dependence is seen
with the inclination, morphological type and stellar mass for these growth
curves. Finally, the median growth curve of O3N2 increases monotonically with
radius. However, at small radii it shows systematically higher values for
galaxies of earlier morphological types and for high stellar mass galaxies.
Applying our aperture corrections to a sample of galaxies from the SDSS
survey at 0.02<=z<=0.3 shows that the average difference between fiber-based
and aperture corrected oxygen abundances, for different galaxy stellar mass and
redshift ranges, reaches typically to ~11%, depending on the abundance
calibration used. This average difference is found to be systematically biased,
though still within the typical uncertainties of oxygen abundances derived from
empirical calibrations. Caution must be exercised when using observations of
galaxies for small radii (e.g. below 0.5R_eff) given the high dispersion shown
around the median growth curves.Comment: Accepted for publication in Ap
The IRX-beta relation on sub-galactic scales in star-forming galaxies of the Herschel Reference Survey
UV and optical surveys are essential to gain insight into the processes
driving galaxy formation and evolution. The rest-frame UV emission is key to
measure the cosmic SFR. However, UV light is strongly reddened by dust. In
starburst galaxies, the UV colour and the attenuation are linked, allowing to
correct for dust extinction. Unfortunately, evidence has been accumulating that
the relation between UV colour and attenuation is different for normal
star-forming galaxies when compared to starburst galaxies. It is still not
understood why star-forming galaxies deviate from the UV colour-attenuation
relation of starburst galaxies. Previous work and models hint that the role of
the shape of the attenuation curve and the age of stellar populations have an
important role. In this paper we aim at understanding the fundamental reasons
to explain this deviation. We have used the CIGALE SED fitting code to model
the far UV to the far IR emission of a set of 7 reasonably face-on spiral
galaxies from the HRS. We have explored the influence of a wide range of
physical parameters to quantify their influence and impact on the accurate
determination of the attenuation from the UV colour, and why normal galaxies do
not follow the same relation as starburst galaxies. We have found that the
deviation can be best explained by intrinsic UV colour differences between
different regions in galaxies. Variations in the shape of the attenuation curve
can also play a secondary role. Standard age estimators of the stellar
populations prove to be poor predictors of the intrinsic UV colour. These
results are also retrieved on a sample of 58 galaxies when considering their
integrated fluxes. When correcting the emission of normal star-forming galaxies
for the attenuation, it is crucial to take into account possible variations in
the intrinsic UV colour as well as variations of the shape of the attenuation
curve.Comment: Accepted for publication in A&A, 18 pages, 14 figures. The paper with
high resolution figures can be downloaded at
http://www.oamp.fr/people/mboquien/HRS/boquien_IRX_beta.pd
The O3N2 and N2 abundance indicators revisited: improved calibrations based on CALIFA and Te-based literature data
The use of IFS is since recently allowing to measure the emission line fluxes
of an increasingly large number of star-forming galaxies both locally and at
high redshift. The main goal of this study is to review the most widely used
empirical oxygen calibrations, O3N2 and N2, by using new direct abundance
measurements. We pay special attention to the expected uncertainty of these
calibrations as a function of the index value or abundance derived and the
presence of possible systematic offsets. This is possible thanks to the
analysis of the most ambitious compilation of Te-based HII regions to date.
This new dataset compiles the Te-based abundances of 603 HII regions extracted
from the literature but also includes new measurements from the CALIFA survey.
Besides providing new and improved empirical calibrations for the gas
abundance, we also present here a comparison between our revisited calibrations
with a total of 3423 additional CALIFA HII complexes with abundances derived
using the ONS calibration by Pilyugin et al. (2010). The combined analysis of
Te-based and ONS abundances allows us to derive their most accurate calibration
to date for both the O3N2 and N2 single-ratio indicators, in terms of all
statistical significance, quality and coverage of the space of parameters. In
particular, we infer that these indicators show shallower abundance
dependencies and statistically-significant offsets compared to those of Pettini
and Pagel (2004), Nagao et al. (2006) and P\'erez-Montero and Contini (2009).
The O3N2 and N2 indicators can be empirically applied to derive oxygen
abundances calibrations from either direct abundance determinations with random
errors of 0.18 and 0.16, respectively, or from indirect ones (but based on a
large amount of data) reaching an average precision of 0.08 and 0.09 dex
(random) and 0.02 and 0.08 dex (systematic; compared to the direct
estimations),respectively.Comment: 12 pages, 5 figures, accepted for publication in A&
Young stellar clusters and associations in M33
We analyse multi-wavelength observations of 32 young star clusters and
associations in M33 with known oxygen abundance (8 < 12 + log(O/H) < 8.7),
using ultraviolet (UV), optical, mid-infrared (MIR), CO (1-0) and 21-cm line
(HI) observations. We derive their spectral energy distribution, and we
determine age, bolometric luminosities, masses and the extinction, by comparing
the multi-band integrated photometry to single-age stellar population models.
The stellar system ages range between 2 and 15 Myr, masses are between 3 x 10^2
and 4 x 10^4 M_sun, and the intrinsic extinction, A_V, varies from 0.3 to 1
mag. We find a correlation between age and extinction, and between the cluster
mass and size. The MIR emission shows the presence of a dust component around
the clusters whose fractional luminosity at 24 um, L_{24}/L_{Bol}, decreases
with the galactocentric distance. However, the total IR luminosity inferred
from L_{24} is smaller than what we derive from the extinction corrections. The
Halpha luminosity predicted by population synthesis models is larger than the
observed one, especially for low-mass systems (M < 10^4 M_sun). Such a
difference is reduced, but not erased, when the incomplete sampling of the
initial mass function (IMF) at the high-mass end is taken into account. Our
results suggest that a non-negligible fraction of UV ionising and non-ionising
radiation is leaking into the ISM outside the HII regions. This would be in
agreement with the large UV and Halpha diffuse fractions observed in M33, but
it implies that stellar systems younger than 3 Myr retain, on average, only 30%
of their Lyman continuum photons. However, the uncertainties on cluster ages
and the stochastic fluctuations of the IMF do not allow to accurately quantify
this issue. We also consider the possibility that this discrepancy is the
consequence of a suppressed or delayed formation of the most massive stars.Comment: 17 pages, 13 figures. Accepted for publications in A&A; v2 --> Table
2 corrected because of a misprint in the FUV magnitude
- …