503 research outputs found
Evolución de los ácidos grasos en el mesocarpo del níspero (Mespilus germanica. L.) a diferentes estados de maduración
The fatty acid composition of medlar (Mespilus germanica L.) varied significantly among the ripening stages sampled at 157, 172 and 187 DAFs (days after full bloom). Twenty-one different fatty acids were detected in preclimacteric fruit and 17 when the climacteric began. Principal fatty acids, determined in medlar fruit harvested from October (157 and 172 DAFs) to November (187 DAF) were mainly palmitic acid (16:0), linoleic acid (18:2n-6), and a-linolenic acid (18:3n-3). While the content of saturated fatty acids [palmitic acid (16:0) and stearic acid (18:0)] increased, the content of the essential polyunsaturated fatty acids [linoleic acid (18:2n-6) and linolenic acid (18:3n-3)] decreased through ripening, in parallel with pulp darkening. The percentage of linoleic acid and a-linolenic acid in ripe, hard fruits was 60.0 and 13.5 % of dry wt at 157 DAF which decreased throughout ripening, remaining at 28.7 and 5.6 % of dry wt, respectively, in the fully softened and darkened pulp. A marked decreases in the double bond index, percentage of unsaturation and the ratio of unsaturation/saturation were also seen throughout the medlar ripening. The contribution of unsaturated fatty acid to the total fatty acid content decreased markedly as the medlar fruit became progressively softer and darkened.La composición en ácidos grasos del níspero (Mespilus germanica L.) varió significativamente entre los estados de maduración muestreados a los 157, 172 y 187 DAFs (días después de la floración). Veinte y un ácidos grasos diferentes fueron detectados en el fruto preclimatérico y 17 cuando comenzó el climaterio. Los ácidos grasos principales encontrados en nísperos, recolectados desde Octubre (157 y 172 DAFs) hasta Noviembre (187 DAF), fueron principalmente ácido palmítico (16:0), ácido linoléico (18:2n-6), y ácido a-linolénico (18:3n-3). En tanto que el contenido en ácidos grasos saturados (ácido palmítico (16:0) y ácido esteárico (18:0)) aumentó, el contenido en ácidos grasos esenciales (ácido linoleico (18:2n-6) y ácido linolénico (18:3n-6)) disminuyó durante la maduración, en paralelo con el oscurecimiento de la pulpa. El porcentaje de ácido linoleico y de ácido a-linolénico en frutos maduros sin reblandecer fue de 60.0 y 13.5 % del peso seco a 157 DAF, disminuyendo durante la maduración, y permaneciendo a 28.7 y 56 % del peso seco, respectivamente, en la pulpa completamente blanda y oscura. También se observó durante la maduración del níspero una marcada disminución en el número de dobles enlaces, en el tanto por ciento de instauración y en la relación instauración / saturación. La contribución de los ácidos grasos insaturados al contenido de ácidos grasos totales disminuyó marcadamente cuando el níspero comenzó progresivamente a reblandecerse y oscurecerse
The mismeasure of conservation
One of the basic purposes of protected areas and other effective area-based conservation interventions is to achieve conservation impact, the sum of avoided biodiversity loss and promoted recovery relative to outcomes without protection. In the context of the Convention on Biological Diversity's negotiations on the post-2020 Global Biodiversity Framework, we find that targets for area-based interventions are framed overwhelmingly with measures that fail to inform decision-makers about impact and that risk diverting limited resources away from achieving it. We show that predicting impact in space and time is feasible and can provide the basis for global guidance for jurisdictions to develop targets for conservation impact and shift investment priorities to areas where impact can be most effectively achieved
Thin Internal Wall Insulation (TIWI) Measuring Energy Performance Improvements in Dwellings Using Thin Internal Wall Insulation Annex D; Moisture Risks of TIWI Laboratory Investigations
What are the effects of nature conservation on human well-being? A systematic map of empirical evidence from developing countries
This is the final version of the article. Available from BioMed Central via the DOI in this record.Background: Global policy initiatives and international conservation organizations have sought to emphasize and strengthen the link between the conservation of natural ecosystems and human development. While many indices have been developed to measure various social outcomes to conservation interventions, the quantity and strength of evidence to support the effects, both positive and negative, of conservation on different dimensions of human well-being, remain unclear, dispersed and inconsistent. Methods: We searched 11 academic citation databases, two search engines and 30 organisational websites for relevant articles using search terms tested with a library of 20 relevant articles. Key informants were contacted with requests for articles and possible sources of evidence. Articles were screened for relevance against predefined inclusion criteria at title, abstract and full text levels according to a published protocol. Included articles were coded using a questionnaire. A critical appraisal of eight systematic reviews was conducted to assess the reliability of methods and confidence in study findings. A visual matrix of the occurrence and extent of existing evidence was also produced. Results: A total of 1043 articles were included in the systematic map database. Included articles measured effects across eight nature conservation-related intervention and ten human well-being related outcome categories. Linkages between interventions and outcomes with high occurrence of evidence include resource management interventions, such as fisheries and forestry, and economic and material outcomes. Over 25 % of included articles examined linkages between protected areas and aspects of economic well-being. Fewer than 2 % of articles evaluated human health outcomes. Robust study designs were limited with less than 9 % of articles using quantitative approaches to evaluate causal effects of interventions. Over 700 articles occurred in forest biomes with less than 50 articles in deserts or mangroves, combined. Conclusions: The evidence base is growing on conservation-human well-being linkages, but biases in the extent and robustness of articles on key linkages persist. Priorities for systematic review, include linkages between marine resource management and economic/material well-being outcomes; and protected areas and governance outcomes. Greater and more robust evidence is needed for many established interventions to better understand synergies and trade-offs between interventions, in particular those that are emerging or contested.This study was made possible by a grant from the Gordon and Betty Moore Foundation to Conservation International (Grant No. 3519). This research was conducted by the Evidence-based Conservation Working Group and financially supported in part by SNAP: Science for Nature and People, a collaboration of The Nature Conservancy, the Wildlife Conservation Society and the National Center for Ecological Analysis and Synthesis (NCEAS)
A human coronavirus responsible for the common cold massively kills dendritic cells but not monocytes
Copyright @ 2012, American Society for Microbiology.Human coronaviruses are associated with upper respiratory tract infections that occasionally spread to the lungs and other organs. Although airway epithelial cells represent an important target for infection, the respiratory epithelium is also composed of an elaborate network of dendritic cells (DCs) that are essential sentinels of the immune system, sensing pathogens and presenting foreign antigens to T lymphocytes. In this report, we show that in vitro infection by human coronavirus 229E (HCoV-229E) induces massive cytopathic effects in DCs, including the formation of large syncytia and cell death within only few hours. In contrast, monocytes are much more resistant to infection and cytopathic effects despite similar expression levels of CD13, the membrane receptor for HCoV-229E. While the differentiation of monocytes into DCs in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4 requires 5 days, only 24 h are sufficient for these cytokines to sensitize monocytes to cell death and cytopathic effects when infected by HCoV-229E. Cell death induced by HCoV-229E is independent of TRAIL, FasL, tumor necrosis factor alpha, and caspase activity, indicating that viral replication is directly responsible for the observed cytopathic effects. The consequence of DC death at the early stage of HCoV-229E infection may have an impact on the early control of viral dissemination and on the establishment of long-lasting immune memory, since people can be reinfected multiple times by HCoV-229E
A future for seafood point-of-origin testing using DNA and stable isotope signatures
Demand for seafood products is increasing worldwide, contributing to ever more complex supply chains and posing challenges to trace their origin and guarantee legal, well-managed, sustainable sources from confirmed locations. While DNA-based methods have proven to be reliable in verifying seafood authenticity at the species level, the verification of geographic origin remains inherently more complex. Both genetic and stable isotope analyses have been employed for determining point-of-origin with varying degrees of success, highlighting that their application can be effective when the right tool is selected for a given application. Developing an a priori prediction of their discrimination power for different applications can help avoid the financial cost of developing inappropriate reference datasets. Here, we reviewed the application of both techniques to seafood point-of-origin for 63 commercial finfish species certified by the Marine Stewardship Council, and showed that, even for those species where baseline data exist, real applications are scarce. To fill these gaps, we synthesised current knowledge on biological and biogeochemical mechanisms that underpin spatial variations in genetic and isotopic signatures. We describe which species’ biological and distribution traits are most helpful in predicting effectiveness of each tool. Building on this, we applied a mechanistic approach to predicting the potential for successful validation of origin to three case study fisheries, using combined genetic and isotopic methodologies to distinguish individuals from certified versus non-certified regions. Beyond ecolabelling applications, the framework we describe could be reproduced by governments and industries to select the most cost-effective techniques
A theory-based framework for understanding the establishment, persistence, and diffusion of community-based conservation
Over decades, biodiversity conservation researchers and practitioners have developed theories and conceptual frameworks to inform the planning, implementation, and evaluation of community-based conservation (CBC). While a diversity of mechanisms for understanding and supporting CBC has helped tailor approaches to local needs and conditions, the absence of a unified lens to understand CBC has limited the capacity for integrating foundational theory into practice more systemically, and for learning across different projects, stakeholders, and institutions. We introduce a theory-based framework called “the CBC framework” that draws upon three foundational theories from sociology, economics, and political science to understand the establishment, persistence, and diffusion of CBC. Experience applying aspects of the framework within different conservation organizations demonstrates how this integrative approach can provide a gateway for practitioners to engage with social science theory to understand the status and context of CBC interventions and efforts. For practitioners, scientists, evaluators, and strategists, the framework can guide the design of CBC interventions and monitoring and evaluation systems to facilitate theory-based learning and enable evidence-informed decision-making. Approaches like the CBC framework that facilitate collaborative planning, evaluation, and research can help better integrate social science theory in conservation practice while increasing the capacity for conservation scientists, practitioners, and stakeholders to learn together and adaptively manage CBC to deliver positive results for both people and nature
A future for seafood point-of-origin testing using DNA and stable isotope signatures
Demand for seafood products is increasing worldwide, contributing to ever more complex supply chains and posing challenges to trace their origin and guarantee legal, well-managed, sustainable sources from confirmed locations. While DNA-based methods have proven to be reliable in verifying seafood authenticity at the species level, the verification of geographic origin remains inherently more complex. Both genetic and stable isotope analyses have been employed for determining point-of-origin with varying degrees of success, highlighting that their application can be effective when the right tool is selected for a given application. Developing an a priori prediction of their discrimination power for different applications can help avoid the financial cost of developing inappropriate reference datasets. Here, we reviewed the application of both techniques to seafood point-of-origin for 63 commercial finfish species certified by the Marine Stewardship Council, and showed that, even for those species where baseline data exist, real applications are scarce. To fill these gaps, we synthesised current knowledge on biological and biogeochemical mechanisms that underpin spatial variations in genetic and isotopic signatures. We describe which species’ biological and distribution traits are most helpful in predicting effectiveness of each tool. Building on this, we applied a mechanistic approach to predicting the potential for successful validation of origin to three case study fisheries, using combined genetic and isotopic methodologies to distinguish individuals from certified versus non-certified regions. Beyond ecolabelling applications, the framework we describe could be reproduced by governments and industries to select the most cost-effective techniques. Graphic abstract: [Figure not available: see fulltext.
Evolution of fatty acids in medlar (<i>Mespilus germanica L.</i>) mesocarp at different stages of ripening
Sediment accumulation rates in subarctic lakes: Insights into age-depth modeling from 22 dated lake records from the Northwest Territories, Canada
Age-depth modeling using Bayesian statistics requires well-informed prior information about the behavior of sediment accumulation. Here we present average sediment accumulation rates (represented as deposition times, DT, in yr/cm) for lakes in an Arctic setting, and we examine the variability across space (intra- and inter-lake) and time (late Holocene). The dataset includes over 100 radiocarbon dates, primarily on bulk sediment, from 22 sediment cores obtained from 18 lakes spanning the boreal to tundra ecotone gradients in subarctic Canada. There are four to twenty-five radiocarbon dates per core, depending on the length and character of the sediment records. Deposition times were calculated at 100-year intervals from age-depth models constructed using the 'classical' age-depth modeling software Clam. Lakes in boreal settings have the most rapid accumulation (mean DT 20±10 yr/cm), whereas lakes in tundra settings accumulate at moderate (mean DT 70±10 yr/cm) to very slow rates, (>100yr/cm). Many of the age-depth models demonstrate fluctuations in accumulation that coincide with lake evolution and post-glacial climate change. Ten of our sediment cores yielded sediments as old as c. 9000cal BP (BP=years before AD 1950). From between c. 9000cal BP and c. 6000cal BP, sediment accumulation was relatively rapid (DT of 20-60yr/cm). Accumulation slowed between c. 5500 and c. 4000cal BP as vegetation expanded northward in response to warming. A short period of rapid accumulation occurred near 1200cal BP at three lakes. Our research will help inform priors in Bayesian age modeling
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