Finely Grinding Cereal Grains in Pelleted Diets Offers Little Improvement in Nursery Pig Growth Performance

Five experiments were conducted to determine the effects of corn particle size and diet form on nursery pig performance and feed preference. In Exp. 1, 192 nursery pigs (PIC 327 × 1050; initially 14.7 lb and 26 d of age) were used in a 35-d experiment. Pens of pigs were balanced by BW and allotted to 1 of 4 treatments with 6 pigs per pen and 8 pens per treatment. The same corn and soybean meal-based diet formulation was used for all treatments. The 2 × 2 factorial consisted of the main effects of corn particle size (400 vs. 700 μm) and diet form (mash vs. pelleted). Pigs fed mash diets had improved overall ADG and greater ADFI during all periods (P \u3c 0.05) and particle size did not impact (P \u3e 0.10) performance. In Exp. 2, a study utilized 96 pigs to evaluate feed preference of pigs consuming mash diets with either 400 or 700 μm corn. Pigs overwhelmingly (P \u3c 0.05) preferred to consume 700 μm corn compared to 400 μm corn (79.3 vs. 20.7%). In Exp. 3, 224 nursery pigs (PIC 327 × 1050; initially 24.1 lb and 40 d of age) were used in a 10-d experiment to determine the effects of corn particle size in pelleted diets on nursery pig performance. Experimental treatments were formed by grinding corn to 1 of 4 different particle sizes (250, 400, 550, or 700 μm). Particle size tended to affect (P \u3c 0.10) ADG in a quadratic manner, but did not impact (P \u3e 0.10) ADFI or F/G. Pigs fed pelleted diets from either 250 or 700 μm corn had poorer ADG than the intermediate treatments. Exp. 4 utilized 91 pigs to evaluate the preference of pigs consuming pelleted diets with either 250 or 700 μm corn from Exp. 3. Even in pelleted form, pigs preferred (P \u3c 0.05) to consume diets manufactured with the coarser particle size corn (58.2 vs. 41.8%). In Exp. 5, 180 nursery pigs (PIC 327 × 1050; initially 15.8 lb and 36 d of age) were used in a 35-d experiment to determine the effects of corn particle size and pelleting on nursery pig growth performance. The 2 × 2 factorial consisted of 2 corn particle sizes (500 μm vs. 750 μm) and two diet forms (mash vs. pelleted). Overall, reducing particle size from 750 to 500 μm did not affect growth performance (P \u3e 0.10). Pelleting reduced (P \u3c 0.05) feed intake, but did not affect ADG or F/G (P \u3e 0.10). These studies suggest that there is little value to be gained by grinding corn to less than 700 microns if fed in pelleted form. Furthermore, our data suggest that, regardless if fed as mash or pellets, pigs prefer to consume diets manufactured with coarser ground corn if given the choice

Evaluating the Accuracy of the 3-Sieve Particle Size Analysis Method Compared to the 12-Sieve Method

The 3-sieve particle size analysis method was developed to estimate the particle size of ground grain within feed mills without the time and expense required for a 12-sieve analysis. The 3-sieve method is more simplistic because it is hand-shaken and uses fewer sieves but has drawbacks because it is not as precise as the 12-sieve method. Because shaking is not automated, technician variation may impact results. Furthermore, the accuracy of the original 3-sieve method has been questioned because the method was developed for corn between 400 to 1,200 μm, and the industry now grinds various grains more finely. Some variations, such as changing the top sieve to a smaller diameter hole or using flow agent, may help improve its accuracy. In this instance, 420 grain samples were used to determine the impact of top sieve size, grain type, technician, and flow agent on the ability of a 3-sieve analytical method to accurately predict the mean particle size determined by a standardized 12-sieve method. The experiment was a 3 × 2 × 2 × 3 factorial with three technicians, two sieve sizes (U.S. No. 12 vs. 16 sieve as the top sieve), flow agent (0 vs. 0.5 g), and three grain types (corn, sorghum, or wheat). Prior to the experiment, all samples were analyzed according to the standard ASAE S319.4 method using a 12-sieve stack with a 15-min tap time and 1 g of flow agent. Linear regression was used to develop individual equations to predict the mean particle size for each of the 3-sieve methods compared to the standard 12-sieve method, and the GLIMMIX procedure of SAS was used to evaluate the impact main effects and interactions on prediction accuracy. All interactions were removed from the model due to insignificance (P \u3e 0.10). Technician, screen size, and flow agent did not affect the accuracy of the prediction equations. Grain was the only main effect of significance (P \u3c 0.05), where the prediction equation overestimated the particle size of wheat by approximately 15 μm and underestimated the particle size of corn by approximately 12 μm. While statistically significant, these variations were deemed to be sufficiently accurate for the 3-sieve method, and separate equations for each grain type were not warranted to retain the simplicity of the method. In summary, technician, sieve size, grain type, and the use of flow agent did not greatly affect the accuracy of the 3-sieve particle size analytical method, so the original method was concluded to be accurate and the preferred method

Evaluating the Effect of Manufacturing Porcine Epidemic Diarrhea Virus (PEDV)-Contaminated Feed on Subsequent Feed Mill Environmental Surface Contamination

This study aimed to utilize the only known pilot feed mill facility approved for pathogenic feed agent use in the United States to evaluate the effect of manufacturing Porcine Epidemic Diarrhea Virus (PEDV)-contaminated feed on subsequent feed mill environmental surface contamination. In this study, PEDV inoculated feed was manufactured and conveyed on equipment along with four subsequent batches of PEDV-free feed. Equipment and environmental surfaces were sampled using swabs and analyzed for the presence of PEDV RNA by PCR. The experiment was replicated three times with decontamination of the feed mill and all equipment between replications. Overall, environmental swabs indicated widespread surface contamination of the equipment and work area after a PEDV contaminated batch of feed was processed. There was little difference in environmental sample cycle threshold (Ct) values after manufacturing each of the subsequent PEDV-negative feed batches. In summary, introduction of PEDV-infected feed into a feed mill will likely result in widespread contamination of equipment and surfaces, even after several batches of PEDV-free feed are produced. Eliminating the PEDV RNA from the feed mill environment was challenging and required procedures that are not practical to apply on a regular basis in a feed mill. This data suggests that it is extremely important to prevent the introduction of PEDV-contaminated feed, ingredients, or other vectors of transmission to minimize PEDV-risk. More research should be conducted to determine if contaminated surfaces can lead to PEDV infectivity and to determine the best feed mill PEDV-decontamination strategies

Serratamolide is a hemolytic factor produced by Serratia marcescens

Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use. © 2012 Shanks et al

Meta-analysis of multidecadal biodiversity trends in Europe

Local biodiversity trends over time are likely to be decoupled from global trends, as local processes may compensate or counteract global change. We analyze 161 long-term biological time series (15-91 years) collected across Europe, using a comprehensive dataset comprising similar to 6,200 marine, freshwater and terrestrial taxa. We test whether (i) local long-term biodiversity trends are consistent among biogeoregions, realms and taxonomic groups, and (ii) changes in biodiversity correlate with regional climate and local conditions. Our results reveal that local trends of abundance, richness and diversity differ among biogeoregions, realms and taxonomic groups, demonstrating that biodiversity changes at local scale are often complex and cannot be easily generalized. However, we find increases in richness and abundance with increasing temperature and naturalness as well as a clear spatial pattern in changes in community composition (i.e. temporal taxonomic turnover) in most biogeoregions of Northern and Eastern Europe. The global biodiversity decline might conceal complex local and group-specific trends. Here the authors report a quantitative synthesis of longterm biodiversity trends across Europe, showing how, despite overall increase in biodiversity metric and stability in abundance, trends differ between regions, ecosystem types, and taxa.peerReviewe

Technological development of Cecropia glaziovi extract pellets by extrusion-spheronization

Cecropia glaziovi Snethl., Urticaceae, is commonly used in South America and is one of the species included in the Brazilian Medicinal Plants Research Program. Pharmacological studies have led to reports of the potential of C. glaziovi as a hypotensive, antiasthmatic and anxiolytic agent. The strict requirements regarding the quality, safety and effectiveness of phytopharmaceutical products represent an enormous challenge in the search for products with a high level of uniformity, reproducibility and stability. The incorporation of dry extracts into multiparticulate dosage forms, such as pellets produced by extrusion/spheronization technology, is a suitable alternative to overcome the lack of technological properties of dry extracts, since they are associated with low flowability and high hygroscopicity. In this study, an optimized dry extract (ODE) of C. glaziovi was incorporated into pellets seeking to decrease the moisture sorption and increase the stability, safety and percentage of the extract in the final product. Pellets containing around 50% of ODE were considered the most technologically viable, offering a narrow particle size distribution, significant improvement in the flowability and compressibility properties, and decrease in the moisture compared with the ODE. In conclusion, pellets containing a high dose of the C. glaviovi extract were successfully prepared, achieving degrees of quality, physical stability and feasibility compatible with the desirable characteristics of a phytopharmaceutical