751 research outputs found

    FETI DDM methodologies for the simulation of high gain Ka-band Transmit arrays (single and dual band)

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    This paper is presenting Finite Element Tearing and Interconnecting (FETI) simulation results of large scale single and dual Ka-band lens transmit-arrays obtained on massively parallel clusters. The computation times observed with the FETI method allow us to consider using it in the optimization phase of such transmit-arrays.info:eu-repo/semantics/publishedVersio

    Differential expression of molecular rust resistance components have distinctive profiles in Coffea arabica - Hemileia vastatrix interactions.

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    Countering the economic hurdle caused by coffee leaf rust disease is most appealing at this time as it has posed a major threat to coffee production around the world. Establishing differential expression profiles at different times following pathogen invasion in both innate and acquired immunities unlocks the molecular components of resistance and susceptibility. Suppression subtractive hybridization (SSH) was used to identify genes differentially over-expressed and repressed during incompatible and compatible interactions between Coffea arabica and Hemileia vastatrix. From 433 clones of expressed sequence tags (ESTs) sequenced, 352 were annotated and categorized of which the proportion of genes expressed during compatible interaction were relatively smaller. The result showed upregulation and downregulation of various genes at 12 and 24 h after pathogen inoculation in both interactions. The use of four different databases in searching for gene homology resulted in different number of similar sequences. BLASTx against EMBL-EBI (European Molecular Biology Laboratory-European Bioinformatics Institute) database being with the maximum (100%) hits for all the annotated sequences. RT-qPCR analysis of seven resistance-signaling genes showed similar expression patterns for most of the genes in both interactions, indicating these genes are involved in basal (nonspecific) defense during which immune reactions are similar. Using SSH, we identified different types of resistance related genes that could be used for further studies towards resistant cultivar development. The potential role of some of the resistance related proteins found were also discussed

    Directed self-assembly of trimeric DNA-bindingchiral miniprotein helicates

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    We propose that peptides are highly versatile platforms for the precise design of supramolecular metal architectures, and particularly, for the controlled assembly of helicates. In this context, we show that the bacteriophage T4 Fibritin foldon (T4Ff) can been engineered on its N-terminus with metal-chelating 2,2′-bipyridine units that stereoselectively assemble in the presence of Fe(II) into parallel, three-stranded peptide helicates with preferred helical orientation. Modeling studies support the proposed self-assembly and the stability of the final helicate. Furthermore, we show that these designed mini-metalloproteins selectively recognize three-way DNA junctions over double-stranded DNAFinancial support from the Spanish grants CTQ2015-70698-R, CTQ2017-87889-P, the Xunta de Galicia (Centro singular de investigación de Galicia accreditation 2016–2019, ED431G/09) and the European Union (European Regional Development Fund - ERDF), is gratefully acknowledged. JG-G, thanks the Spanish MINECO for his FPI fellowship, GB thanks the ERC for her EU METALIC-II 2013-2442/001-001-EMA2 mobility scheme fellowship, and GS. thanks the Universitat Autònoma de Barcelona for its support to his PhD. J-DM and GS are thankful for the support given by the Generalitat de Catalunya 2017SGR1323. Support of COST Action CM1306 is kindly acknowledged. MEV, also wish to acknowledge the generous support by the Fundación Asociación Española Contra el Cáncer AECC (IDEAS197VAZQ grant)S

    Seismic tomography of the North Anatolian Fault: New insights into structural heterogeneity along a continental strike-slip fault

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    Knowledge of the structure of continental strike-slip faults within the lithosphere is essential to understand where the deformation occurs and how strain localizes with depth. With the aim to improve the constraints on the lower crust and upper mantle structure of a major continental strike-slip fault, we present a high-resolution teleseismic tomography of the North Anatolian Fault Zone (NAFZ) in Turkey. Our results highlight the presence of a relatively high velocity body between the two branches of the fault and significant along-strike variations in the NAFZ velocity structure over distances of ~20 km. We interpret these findings as evidence of laterally variable strain focussing caused by preexisting heterogeneity. Low velocities observed in the crust and upper mantle beneath the NAFZ support the presence of a narrow shear zone widening in the upper mantle, where we constrain its width to be ~50 km.Major funding was provided by the UK Natural Environment Research Council (NERC) under grant NE/I028017/1. Equipment was provided and supported by the NERC Geophysical Equipment Facility (SEIS-UK). This project is also supported by Bogaziçi University Scientific ˘ Research Projects (BAP) under grant 6922 and Turkish State Planning Organization (DPT) under the TAM project, number 2007K120610

    An example of secondary fault activity along the North Anatolian Fault on the NE Marmara Sea Shelf, NW Turkey

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    Seismic data on the NE Marmara Sea Shelf indicate that a NNE-SSW-oriented buried basin and ridge system exist on the sub-marine extension of the Paleozoic Rocks delimited by the northern segment of the North Anatolian Fault (NS-NAF), while seismic and multi-beam bathymetric data imply that four NW-SE-oriented strike-slip faults also exist on the shelf area. Seismic data indicate that NW-SE-oriented strike-slip faults are the youngest structures that dissect the basin-ridge system. One of the NW-SE-oriented faults (F1) is aligned with a rupture of the North Anatolian Fault (NAF) cutting the northern slope of the Cinarcik Basin. This observation indicates that these faults have similar characteristics with the NS-NAF along the Marmara Sea. Therefore, they may have a secondary relation to the NAF since the principle deformation zone of the NAF follows the Marmara Trough in that region. The seismic energy recorded on these secondary faults is much less than that on the NAF in the Marmara Sea. These faults may, however, produce a large earthquake in the long term

    3-D sediment-basement tomography of the Northern Marmara trough by a dense OBS network at the nodes of a grid of controlled source profiles along the North Anatolian fault

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    A 3-D tomographic inversion of first arrival times of shot profiles recorded by a dense 2-D OBS network provides an unprecedented constraint on the P-wave velocities heterogeneity of the upper-crustal part of the North Marmara Trough (NMT), over a region of 180 km long by 50 km wide. One of the specific aims of this controlled source tomography is to provide a 3-D initial model for the local earthquake tomography (LET). Hence, in an original way, the controlled source inversion has been performed by using a code dedicated to LET. After several tests to check the results trade-off with the inversion parameters, we build up a 3-D a priori velocity model, in which the sea-bottom topography, the acoustic and the crystalline basements and the Moho interfaces have been considered. The reliability of the obtained features has been checked by checkerboard tests and also by their comparison with the deep-penetration multichannel seismic profiles, and with the wide-angle reflection and refraction modelled profiles. This study provides the first 3-D view of the basement topography along the active North Anatolian fault beneath the Marmara Sea, even beneath the deepest part of three sedimentary basins of NMT. Clear basement depressions reaching down 6 km depth below the sea level (bsl) have been found beneath these basins. The North Imralı Basin located on the southern continental shelf is observed with a similar sedimentary thickness as its northern neighbours. Between Central and Çınarcık basins, the Central High rises up to 3 km depth below (bsl). Its crest position is offset by 10 km northwestward relatively to the bathymetric crest. On the contrary, Tekirdağ and Central basins appear linked, forming a 60-km-long basement depression. Beneath the bathymetric relief of Western High low velocities are observed down to 6 km depth (bsl) and no basement high have been found. The obtained 3-D Vp heterogeneity model allows the consideration of the 3-D supracrustal heterogeneity into the future earthquake relocations in this region. The topographic map of the pre-kinematic basement offers the possibility to take into account the locking depth variations in future geohazard estimations by geomechanical modelling in this region

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    Here we present a formal description of Biremis panamae Barka, Witkowski et Weisenborn sp. nov., which was isolated from the marine littoral environment of the Pacific Ocean coast of Panama. The description is based on morphology (light and electron microscopy) and the rbcL, psbC and SSU sequences of one clone of this species. The new species is included in Biremis due to its morphological features; i.e. two marginal rows of foramina, chambered striae, and girdle composed of numerous punctate copulae. The new species also possesses a striated valve face which is not seen in most known representatives of marine littoral Biremis species. In this study we also present the relationship of Biremis to other taxa using morphology, DNA sequence data and observations of auxosporulation. Our results based on these three sources point to an evolutionary relationship between Biremis, Neidium and Scoliopleura. The unusual silicified incunabular caps present in them are known otherwise only in Muelleria, which is probably related to the Neidiaceae and Scoliotropidaceae. We also discuss the relationship between Biremis and the recently described Labellicula and Olifantiella

    Marker-assisted recurrent selection for pyramiding leaf rust and coffee berry disease resistance alleles in Coffea Arabica L.

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    Abstract: In this study, marker-assisted recurrent selection was evaluated for pyramiding resistance gene alleles against coffee leaf rust (CLR) and coffee berry diseases (CBD) in Coffea arabica. A total of 144 genotypes corresponding to 12 hybrid populations from crosses between eight parent plants with desired morphological and agronomic traits were evaluated. Molecular data were used for cross-certification, diversity study and resistance allele marker-assisted selection (MAS) against the causal agent of coffee leaf rust (Hemileia vastatrix) and coffee berry disease (Colletotrichum kahawae). In addition, nine morphological and agronomic traits were evaluated to determine the components of variance, select superior hybrids, and estimate genetic gain. From the genotypes evaluated, 134 were confirmed as hybrids. The genetic diversity between and within populations was 75.5% and 24.5%, respectively, and the cluster analysis revealed three primary groups. Pyramiding of CLR and CBD resistance genes was conducted in 11 genotypes using MAS. A selection intensity of 30% resulted in a gain of over 50% compared to the original population. Selected hybrids with increased gain also showed greater genetic divergence in addition to the pyramided resistance alleles. The strategies used were, therefore, efficient to select superior coffee hybrids for recurrent selection programs and could be used as a source of resistance in various crosses
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