Characteristics of P2X7 receptors from human B lymphocytes expressed in Xenopus oocytes

AbstractHuman B lymphocytes express an ATP-gated ion channel (P2Z receptor), which shares similarities with the recently identified P2X7 receptor. Using gene specific primers, we have now isolated P2X7 cDNA from the total RNA of human B lymphocytes. This hP2X7 receptor subtype was expressed in Xenopus oocytes and electrophysiologically characterized. The hP2X7 receptor is similar to, but does not completely match, P2Z of human B cells. The hP2X7 receptors resemble the P2Z receptors with regard to the ATP concentration of half maximal activation, reproducibility, permeation characteristics and lack of desensitization of the ATP-evoked currents. However, in contrast to the native lymphocytic P2Z receptor, the time course of activation of hP2X7 displayed an additional linearly increasing current component. Furthermore, a second, small and slowly deactivating current component exists only in hP2X7 expressed in oocytes. The activation and deactivation kinetics as well as permeation characteristics of hP2X7 are different from rat P2X7 recently expressed in oocytes. Unlike in mammalian cells, hP2X7 expressed in Xenopus oocytes is not sufficient to induce large non-selective pores

Network platform for sound information analyzis

A new technique of audio information processing for automatic analysis and classification of records, including speech, has been proposed. The technique is based on information representation in the form of associative semantic (cognitive) network structure and deals with amplitude and frequency layers both

The Milky Way Nuclear Star Cluster

In the center of the Milky Way, as well as in many other galaxies, a compact star cluster around a very massive black hole is observed. One of the possible explanations for the formation of such Nuclear Star Clusters is based on the 'merging' of globular clusters in the inner galactic potential well. By mean of sophisticated N-body simulations, we checked the validity of this hypothesis and found that it may actually has been the one leading to the formation of the Milky Way Nuclear Star Cluster.Comment: 4 pages, 2 figures, proceedings of "Stellar Clusters and Associations - A RIA workshop on GAIA", 23-27 May 2011, Granada, Spai

The Herschel Exploitation of Local Galaxy Andromeda (HELGA). VI. The distribution and properties of molecular cloud associations in M31

In this paper we present a catalog of Giant Molecular Clouds (GMCs) in the An- dromeda (M31) galaxy extracted from the Herschel Exploitation of Local Galaxy An- dromeda (HELGA) dataset. GMCs are identified from the Herschel maps using a hierarchical source extraction algorithm. We present the results of this new catalog and characterise the spatial distribution and spectral energy properties of its clouds based on the radial dust/gas properties found by Smith et al (2012). 326 GMCs in the mass range 104 − 107 M⊙ are identified, their cumulative mass distribution is found to be proportional to M −2.34 in agreement with earlier studies. The GMCs appear to follow the same cloud mass to LCO correlation observed in the Milky Way. However, comparison between this catalog and interferometry studies also shows that the GMCs are substructured below the Herschel resolution limit suggesting that we are observing associations of GMCs. Following Gordon et al. (2006), we study the spatial structure of M31 by splitting the observed structure into a set of spiral arms and offset rings. We fit radii of 10.3 and 15.5 kpc to the two most prominent rings. We then fit a logarithmic spiral with a pitch angle of 8fdg9 to the GMCs not associated with either ring. Last, we comment on the effects of deprojection on our results and investigate the effect different models for M31's inclination will have on the projection of an unperturbed spiral arm system

The Herschel Virgo Cluster Survey: VI. The far-infrared view of M87

The origin of the far-infrared emission from the nearby radio galaxy M87 remains a matter of debate. Some studies find evidence of a far-infrared excess due to thermal dust emission, whereas others propose that the far-infrared emission can be explained by synchrotron emission without the need for an additional dust emission component. We present Herschel PACS and SPIRE observations of M87, taken as part of the science demonstration phase observations of the Herschel Virgo Cluster Survey. We compare these data with a synchrotron model based on mid-infrared, far-infrared, submm and radio data from the literature to investigate the origin of the far-infrared emission. Both the integrated SED and the Herschel surface brightness maps are adequately explained by synchrotron emission. At odds with previous claims, we find no evidence of a diffuse dust component in M87, which is not unexpected in the harsh X-ray environment of this radio galaxy sitting at the core of the Virgo Cluster.Comment: Letter accepted for publication in A&A (Herschel special issue

Activation kinetics of single P2X receptors

After the primary structure of P2X receptors had been identified, their function had to be characterized on the molecular level. Since these ligand-gated ion channels become activated very quickly after binding of ATP, methods with adequate time resolution have to be applied to investigate the early events induced by the agonist. Single-channel recordings were performed to describe conformational changes on P2X2, P2X4, and P2X7 receptors induced by ATP and also by allosteric receptor modifiers. The main results of these studies and the models of P2X receptor kinetics derived from these observations are reviewed here. The investigation of purinoceptors by means of the patch clamp technique following site-directed mutagenesis will probably reveal more details of P2X receptor function at the molecular level