43 research outputs found
EFEITOS DA ATIVIDADE FÍSICA NOS NÍVEIS DE GLICOSE SANGUÍNEA EM RATOS WISTAR
The aim of this study was to verify the chronic effects of physical activity in an enriched environment on blood glucose levels in Wistar rats. Male rats were used, paired by weight and age. They were randomly assigned to 2 groups, according to the treatment received: sedentary controls (n=8) and experimental physical activity (n=8). Blood glucose was measured 24 hours before (T1) and after (T2) 45 sessions of physical activity (one daily session) in an enriched environment. The experimental group that performed spontaneous physical activity for 30 minutes during 45 sessions. Checking the descriptive data and the inferential analysis of blood glucose levels, in the experimental group there was a decrease in this variable, whereas in the control group there was an increase, however, in the two situations there was no significant difference. The outlying result of the delta blood glucose values called attention, as there was a significant difference (p=0.039) in the comparison of the groups, this finding shows that physical activity in an enriched environment can promote relevant results from the perspective of health. Chronically, physical activity in an enriched environment appears to alter glucose sensitivity in response to exercise. These recorded effects may favor the use of this model of physical activity in housing with an enriched environment in experimental studies with rats of the Wistar lineage, as there is an extensive methodological obstacle of similarity in the model of physical activity in rodents similar to those practiced with humans, thus, there may have been an alignment of pre-clinical studies with clinical studies involving physical activity as an experimental proposal.O objetivo desse estudo foi verificar os efeitos crônicos da atividade física em ambiente enriquecido nos níveis de glicose sanguínea em ratos Wistar. Foram utilizados ratos machos, pareados por peso e idade. Eles foram distribuídos aleatoriamente em 2 grupos, de acordo com o tratamento recebido: sedentários controles (n=8) e atividade física experimental (n=8). Foi realizada mensuração da glicose sanguínea 24 horas (T1) antes e após (T2) 45 sessões de atividade física (uma sessão diária) em ambiente enriquecido. O grupo experimental que realizou atividade física espontânea por 30 minutos durante 45 sessões. Verificando os dados descritivos e a análise inferencial dos níveis de glicose sanguínea, no grupo experimental ocorreu diminuição dessa variável, já no grupo controle incidiu no aumento, porém, nas duas situações não ocorreu diferença significativa. O resultado dos valores delta da glicose sanguínea chamaram atenção, pois ocorreu diferença significativa (p=0,039) na comparação dos grupos, tal achado mostra que a atividade física em ambiente enriquecido pode promover resultados relevantes na perspectiva da saúde. Cronicamente, a atividade física em ambiente enriquecido parece alterar a sensibilidade à glicose em resposta ao exercício. Esses efeitos registrados podem favorecer a utilização desse modelo de atividade física em alojamento com ambiente enriquecido em estudos experimentais com ratos da linhagem Wistar, pois existe um extenso obstáculo metodológico de similaridade de modelo de atividade física em roedores, semelhantes às praticadas com seres humanos, assim, pode ter ocorrido um aproximação de alinhamento de estudos pré-clínicos com estudos clínicos que envolvem a atividade física como proposta experimental
Light, Ethylene and Auxin Signaling Interaction Regulates Carotenoid Biosynthesis During Tomato Fruit Ripening
Light signaling and plant hormones, particularly ethylene and auxins, have been identified as important regulators of carotenoid biosynthesis during tomato fruit ripening. However, whether and how the light and hormonal signaling cascades crosstalk to control this metabolic route remain poorly elucidated. Here, the potential involvement of ethylene and auxins in the light-mediated regulation of tomato fruit carotenogenesis was investigated by comparing the impacts of light treatments and the light-hyperresponsive high pigment-2 (hp2) mutation on both carotenoid synthesis and hormonal signaling. Under either light or dark conditions, the overaccumulation of carotenoids in hp2 ripening fruits was associated with disturbed ethylene production, increased expression of genes encoding master regulators of ripening and higher ethylene sensitivity and signaling output. The increased ethylene sensitivity observed in hp2 fruits was associated with the differential expression of genes encoding ethylene receptors and downstream signaling transduction elements, including the downregulation of the transcription factor ETHYLENE RESPONSE FACTOR.E4, a repressor of carotenoid synthesis. Accordingly, treatments with exogenous ethylene promoted carotenoid biosynthetic genes more intensively in hp2 than in wild-type fruits. Moreover, the loss of HP2 function drastically altered auxin signaling in tomato fruits, resulting in higher activation of the auxin-responsive promoter DR5, severe down-regulation of AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) genes and altered accumulation of AUXIN RESPONSE FACTOR (ARF) transcripts. Both tomato ARF2 paralogues (Sl-ARF2a and SlARF2b) were up-regulated in hp2 fruits, which agrees with the promotive roles played by these ARFs in tomato fruit ripening and carotenoid biosynthesis. Among the genes differentially expressed in hp2 fruits, the additive effect of light treatment and loss of HP2 function was particularly evident for those encoding carotenoid biosynthetic enzymes, ethylene-related transcription factors, Aux/IAAs and ARFs. Altogether, the data uncover the involvement of ethylene and auxin as part of the light signaling cascades controlling tomato fruit metabolism and provide a new link between light signaling, plant hormone sensitivity and carotenoid metabolism in ripening fruits
The Bom Santo Cave (Lisbon, Portugal): catchment, diet, and patterns of mobility of a Middle Neolithic population
The study of the Bom Santo Cave (central Portugal), a Neolithic cemetery, indicates a complex social, palaeoeconomic, and population scenario. With isotope, aDNA, and provenance, analyses of raw materials coupled with stylistic variability of material culture items and palaeogeographical data, light is shed on the territory and social organization of a population dated to 3800-3400 cal BC, i.e. the Middle Neolithic. Results indicate an itinerant farming, segmentary society, where exogamic practices were the norm. Its lifeway may be that of the earliest megalithic builders of the region, but further research is needed to correctly evaluate the degree of this community's participation in such a phenomenon
Viral genetic clustering and transmission dynamics of the 2022 mpox outbreak in Portugal
Pathogen genome sequencing during epidemics enhances our ability to identify and understand suspected clusters and investigate their relationships. Here, we combine genomic and epidemiological data of the 2022 mpox outbreak to better understand early viral spread, diversification and transmission dynamics. By sequencing 52% of the confirmed cases in Portugal, we identified the mpox virus sublineages with the highest impact on case numbers and fitted them into a global context, finding evidence that several international sublineages probably emerged or spread early in Portugal. We estimated a 62% infection reporting rate and that 1.3% of the population of men who have sex with men in Portugal were infected. We infer the critical role played by sexual networks and superspreader gatherings, such as sauna attendance, in the dissemination of mpox virus. Overall, our findings highlight genomic epidemiology as a tool for the real-time monitoring and control of mpox epidemics, and can guide future vaccine policy in a highly susceptible population.info:eu-repo/semantics/publishedVersio
MAMMALS IN PORTUGAL : A data set of terrestrial, volant, and marine mammal occurrences in P ortugal
Mammals are threatened worldwide, with 26% of all species being includedin the IUCN threatened categories. This overall pattern is primarily associatedwith habitat loss or degradation, and human persecution for terrestrial mam-mals, and pollution, open net fishing, climate change, and prey depletion formarine mammals. Mammals play a key role in maintaining ecosystems func-tionality and resilience, and therefore information on their distribution is cru-cial to delineate and support conservation actions. MAMMALS INPORTUGAL is a publicly available data set compiling unpublishedgeoreferenced occurrence records of 92 terrestrial, volant, and marine mam-mals in mainland Portugal and archipelagos of the Azores and Madeira thatincludes 105,026 data entries between 1873 and 2021 (72% of the data occur-ring in 2000 and 2021). The methods used to collect the data were: live obser-vations/captures (43%), sign surveys (35%), camera trapping (16%),bioacoustics surveys (4%) and radiotracking, and inquiries that represent lessthan 1% of the records. The data set includes 13 types of records: (1) burrowsjsoil moundsjtunnel, (2) capture, (3) colony, (4) dead animaljhairjskullsjjaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8),observation in shelters, (9) photo trappingjvideo, (10) predators dietjpelletsjpine cones/nuts, (11) scatjtrackjditch, (12) telemetry and (13) vocalizationjecholocation. The spatial uncertainty of most records ranges between 0 and100 m (76%). Rodentia (n=31,573) has the highest number of records followedby Chiroptera (n=18,857), Carnivora (n=18,594), Lagomorpha (n=17,496),Cetartiodactyla (n=11,568) and Eulipotyphla (n=7008). The data setincludes records of species classified by the IUCN as threatened(e.g.,Oryctolagus cuniculus[n=12,159],Monachus monachus[n=1,512],andLynx pardinus[n=197]). We believe that this data set may stimulate thepublication of other European countries data sets that would certainly contrib-ute to ecology and conservation-related research, and therefore assisting onthe development of more accurate and tailored conservation managementstrategies for each species. There are no copyright restrictions; please cite thisdata paper when the data are used in publications.info:eu-repo/semantics/publishedVersio
Compostos fenólicos e capacidade antioxidante em frutos de tomateiros mutantes fotomorfogenéticos
The inventory of geological heritage of the state of São Paulo, Brazil: Methodological basis, results and perspectives
An inventory of geological sites based on solid and clear criteria is a first step for any geoconservation strategy. This paper describes the method used in the geoheritage inventory of the State of São Paulo, Brazil, and presents its main results. This inventory developed by the geoscientific community aimed to identify geosites with scientific value in the whole state, using a systematic approach. All 142 geosites representative of 11 geological frameworks were characterised and quantitatively evaluated according to their scientific value and risk of degradation, in order to establish priorities for their future management. An online database of the inventory is under construction, which will be available to be easily consulted and updated by the geoscientific community. All data were made available to the State Geological Institute as the backbone for the implementation of a future state geoconservation strategy.The authors acknowledge the Science Without Borders Programme, Process 075/2012, which supported this study and the São Paulo Research Foundation (FAPESP), Process 2011/17261-6. We also thanks C. Mazoca for his help with maps and figures.info:eu-repo/semantics/acceptedVersio
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4
While the increasing availability of global databases on ecological communities has advanced our knowledge
of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In
the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of
Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus
crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced
environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian
Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by
2050. This means that unless we take immediate action, we will not be able to establish their current status,
much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio