16 research outputs found
Two-dimensional combinatorial screening and the RNA Privileged Space Predictor program efficiently identify aminoglycosideāRNA hairpin loop interactions
Herein, we report the identification of RNA hairpin loops that bind derivatives of kanamycin A, tobramycin, neamine, and neomycin B via two-dimensional combinatorial screening, a method that screens chemical and RNA spaces simultaneously. An arrayed aminoglycoside library was probed for binding to a 6-nucleotide RNA hairpin loop library (4096 members). Members of the loop library that bound each aminoglycoside were excised from the array, amplified and sequenced. Sequences were analyzed with our newly developed RNA Privileged Space Predictor (RNA-PSP) program, which analyzes selected sequences to identify statistically significant trends. RNA-PSP identified the following unique trends: 5ā²UNNNC3ā² loops for the kanamycin A derivative (where N is any nucleotide); 5ā²UNNC3ā² loops for the tobramycin derivative; 5ā²UNC3ā² loops for the neamine derivative; and 5ā²UNNG3ā² loops for the neomycin B derivative. The affinities and selectivities of a subset of the ligandāhairpin loop interactions were determined. The selected interactions have Kd values ranging from 10 nM to 605 nM. Selectivities ranged from 0.4 to >200-fold. Interestingly, the results from RNA-PSP are able to qualitatively predict specificity based on overlap between the RNA sequences selected for the ligands. These studies expand the information available on small moleculeāRNA motif interactions, which could be useful to design ligands targeting RNA
Aberrant left coronary artery arising from the right sinus of Valsalva: Case reports of a rare entity
Evaluation of the value of serum glycomics (GlycoCirrhoTest) for risk prediction of hepatocellular carcinoma in compensated cirrhosis.
2-Deoxystreptamine conjugates by truncation-derivatization of neomycin
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Phenotypes and genotypes in non-consanguineous and consanguineous primary microcephaly : high incidence of epilepsy
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Clinical features and evolution of bacterial infection-related acute-on-chronic liver failure
Bacterial infections can trigger the development of organ failure/s and acute-on-chronic liver failure (ACLF). Different geographic variations in their bacteriology and clinical practices could lead to different epidemiology, phenotypes and outcomes of ACLF around the world. The aim of the study was to evaluate regional differences in bacterial infection-related ACLF in patients with cirrhosis admitted to hospital
Exploring the development of a decision support system (DSS) to prioritize engineered nanoparticles for risk assessment
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Epidemiology and Effects of Bacterial Infections in Patients With Cirrhosis Worldwide
Bacterial infections are common and life-threatening in patients with cirrhosis. Little is known about the epidemiology of bacterial infections in different regions. We performed a multicenter prospective intercontinental study to assess the prevalence and outcomes of bacterial and fungal infections in patients with cirrhosis