296 research outputs found
Synthetic heterochromatin bypasses RNAi and centromeric repeats to establish functional centromeres
In the central domain of fission yeast centromeres, the kinetochore is assembled on CENP-A cnp1 nucleosomes. Normally, small interfering RNAs generated from flanking outer repeat transcripts direct histone H3 lysine 9 methyltransferase Clr4 to homologous loci to form heterochromatin. Outer repeats, RNA interference (RNAi), and centromeric heterochromatin are required to establish CENP-A Cnpl chromatin. We demonstrated that tethering Clr4 via DNA-binding sites at euchromatic loci induces heterochromatin assembly, with or without active RNAi. This synthetic heterochromatin completely substitutes for outer repeats on plasmid-based minichromosomes, promoting de novo CENP-A Cnpl and kinetochore assembly, to allow their mitotic segregation, even with RNAi inactive. Thus, the role of outer repeats in centromere establishment is simply the provision of RNAi substrates to direct heterochromatin formation; H3K9 methylation-dependent heterochromatin is alone sufficient to form functional centromeres.SCOPUS: ar.jinfo:eu-repo/semantics/publishe
Solar-like oscillations in the G8 V star tau Ceti
We used HARPS to measure oscillations in the low-mass star tau Cet. Although
the data were compromised by instrumental noise, we have been able to extract
the main features of the oscillations. We found tau Cet to oscillate with an
amplitude that is about half that of the Sun, and with a mode lifetime that is
slightly shorter than solar. The large frequency separation is 169 muHz, and we
have identified modes with degrees 0, 1, 2, and 3. We used the frequencies to
estimate the mean density of the star to an accuracy of 0.45% which, combined
with the interferometric radius, gives a mass of 0.783 +/- 0.012 M_sun (1.6%).Comment: accepted for publication in A&
Executive functioning in preschool children affected by autism spectrum disorder: A pilot study
Introduction: Autism spectrum disorders (ASD) are a complex set of neurological dysfunction and development characterized by deficits in social and non-verbal interaction. Few studies have explored the executive functioning in ASD preschoolers. The aim of this pilot study is the assessment of executive functioning in preschool children with ASD. Material and methods: 8 ASD children (7 males, 1 female) mean age 3.09 (SD \ub1 0.83 years) were enrolled in the study and compared with a control group of 15 typically developing children (12 males, 3 females) (mean age 24.3 \ub1 0.61). All subjects underwent assessment of executive functioning with the BRIEF-P test. Results: The two groups were matched for age (p = 0.625) and gender (p = 0.900). Table 1 shows the comparison between the two groups at the BRIEF-P, with significantly higher scores on all subscales of ASD children compared with controls. Conclusions: Despite the small sample examined the results of this study agree with what is already known in the literature confirm the presence of a significant deficit in executive functions of subjects with ASD emphasizing for the first time the emergence of such problems at an early stage of development, but demanding further studies to confirm this
Monoclonal anti-CD18 antibody prevents transcellular biosynthesis of cysteinyl leukotrienes in vitro and in vivo and protects against leukotriene-dependent increase in coronary vascular resistance and myocardial stiffness
Background - Cysteinyl leukotrienes (cys-LT) can constrict small and large vessels and increase vascular permeability. Formation of cys-LT arising from polymorphonuclear leukocytes (PMNL) and endothelial cell cooperation (transcellular synthesis) led to the hypothesis that PMNL-endothelial cell adhesion may represent a key step toward the formation of vasoactive cys-LT. Methods and Results - We studied the effect of pretreatment with a monoclonal antibody directed against the CD18 subunit of PMNL \u3b22-integrin on the synthesis of cys-LT in a PMNL-perfused isolated rabbit heart in vitro and in a model of permanent ligature of the left descending coronary artery in the rabbit in vivo. Challenge of PMNL-perfused rabbit hearts with formyl-met-leu- phe (0.3 \u3bcmol/L) caused synthesis of cys-LT and increase in coronary perfusion pressure that were prevented by the anti-CD18 antibody. Similar results were obtained with the use of A-23187 (0.5 \u3bcmol/L) as a challenge. Persistence of PMNL-associated myeloperoxidase activity in the perfusion buffer was observed in the presence of the anti-CD18 antibody, indicating decreased PMNL infiltration. Coronary artery ligature in vivo increased urinary excretion of leukotriene E4, supporting the activation of the 5- lipoxygenase pathway during experimental acute myocardial infarction. Pretreatment with the anti-CD18 antibody (1 mg/kg) prevented the increase in leukotriene E4 excretion. Conclusions - These data support the importance of adhesion in promoting cys-LT formation, originating from PMNL-endothelial cell cooperation, and contributing to myocardial stiffness and increased coronary resistance
Proteolysis in salmon ( Salmo salar ) during cold storage : Effects of storage time and smoking process
The Italian Museo Nazionale dell'Antartide: National and International Contribution to education, outreach and training
Ultra-precise Masses and Magnitudes for the Gliese 268 M-dwarf Binary
Recent advances in astrometry using interferometry and precision radial velocity techniques combined allow for a significant improvement in the precision of masses of M‐dwarf stars in visual systems. We report recent astrometric observations of Gliese 268, an M‐dwarf binary with a 10.4 day orbital period, with the IOTA interferometer and radial velocity observations with the ELODIE instrument. Combining these measurements leads to preliminary masses of the constituent stars with uncertainties of 0.4%. The masses of the components are 0.22596±0.00084 M_⊙ for the primary and 0.19230±0.00071 M_⊙ for the secondary. The system parallax is determined by these observations to be 0.1560±.0030 arcsec (2.0% uncertainty) and is within Hipparcos error bars (0.1572±.0033). We tested these physical parameters, along with the near‐infrared luminosities of the stars, against stellar evolution models for low‐mass stars. Discrepancies between the measured and theoretical values point toward a low‐level departure from the predictions. These results are among the most precise masses measured for visual binaries
A near-infrared interferometric survey of debris disc stars. II. CHARA/FLUOR observations of six early-type dwarfs
High-precision interferometric observations of six early-type main sequence
stars known to harbour cold debris discs have been obtained in the
near-infrared K band with the FLUOR instrument at the CHARA Array. The measured
squared visibilities are compared to the expected visibility of the stellar
photospheres based on theoretical photospheric models taking into account
rotational distortion, searching for potential visibility reduction at short
baselines due to circumstellar emission. Our observations bring to light the
presence of resolved circumstellar emission around one of the six target stars
(zeta Aql) at the 5 sigma level. The morphology of the emission source cannot
be directly constrained because of the sparse spatial frequency sampling of our
interferometric data. Using complementary adaptive optics observations and
radial velocity measurements, we find that the presence of a low-mass companion
is a likely origin for the excess emission. The potential companion has a
K-band contrast of four magnitudes, a most probable mass of about 0.6 Msun, and
is expected to orbit between about 5.5 AU and 8 AU from its host star assuming
a purely circular orbit. Nevertheless, by adjusting a physical debris disc
model to the observed Spectral Energy Distribution of the zeta Aql system, we
also show that the presence of hot dust within 10 AU from zeta Aql, producing a
total thermal emission equal to 1.69 +- 0.31% of the photospheric flux in the K
band, is another viable explanation for the observed near-infrared excess. Our
re-interpretation of archival near- to far-infrared photometric measurements
shows however that cold dust is not present around zeta Aql at the sensitivity
limit of the IRS and MIPS instruments onboard Spitzer, and urges us to remove
zeta Aql from the category of bona fide debris disc stars.Comment: 14 pages, accepted for publication in A&
Hot circumstellar material resolved around β Pic with VLTI/PIONIER
Aims. We aim at resolving the circumstellar environment around β Pic in the near-infrared in order to study the inner planetary system (<200 mas, i.e., ~4 AU).
Methods. Precise interferometric fringe visibility measurements were obtained over seven spectral channels dispersed across the H band with the four-telescope VLTI/PIONIER interferometer. Thorough analysis of interferometric data was performed to measure the stellar angular diameter and to search for circumstellar material.
Results. We detected near-infrared circumstellar emission around β Pic that accounts for 1.37% ± 0.16% of the near-infrared stellar flux and that is located within the field-of-view of PIONIER (i.e., ~200 mas in radius). The flux ratio between this excess and the photosphere emission is shown to be stable over a period of 1 year and to vary only weakly across the H band, suggesting that the source is either very hot (≳1500 K) or dominated by the scattering of the stellar flux. In addition, we derive the limb-darkened angular diameter of β Pic with an unprecedented accuracy (θ_LD= 0.736 ± 0.019 mas).
Conclusions. The presence of a small H-band excess originating in the vicinity of β Pic is revealed for the first time thanks to the high-precision visibilities enabled by VLTI/PIONIER. This excess emission is likely due to the scattering of stellar light by circumstellar dust and/or the thermal emission from a yet unknown population of hot dust, although hot gas emitting in the continuum cannot be firmly excluded
A Submillimetre Search for Cold Extended Debris Disks in the Beta Pictoris Moving Group
The Beta Pictoris Moving Group is a nearby stellar association of young
(12Myr) co-moving stars including the classical debris disk star beta Pictoris.
Due to their proximity and youth they are excellent targets when searching for
submillimetre emission from cold, extended, dust components produced by
collisions in Kuiper-Belt-like disks. They also allow an age independent study
of debris disk properties as a function of other stellar parameters. We
observed 7 infrared-excess stars in the Beta Pictoris Moving Group with the
LABOCA bolometer array, operating at a central wavelength of 870 micron at the
12-m submillimetre telescope APEX. The main emission at these wavelengths comes
from large, cold dust grains, which constitute the main part of the total dust
mass, and hence, for an optically thin case, make better estimates on the total
dust mass than earlier infrared observations. Fitting the spectral energy
distribution with combined optical and infrared photometry gives information on
the temperature and radial extent of the disk. From our sample, beta Pic,
HD181327, and HD172555 were detected with at least 3-sigma certainty, while all
others are below 2-sigma and considered non-detections. The image of beta Pic
shows an offset flux density peak located near the south-west extension of the
disk, similar to the one previously found by SCUBA at the JCMT. We present SED
fits for detected sources and give an upper limit on the dust mass for
undetected ones. We find a mean fractional dust luminosity f_dust=11x10^{-4} at
t=12Myr, which together with recent data at 100Myr suggests an f_dust propto
t^{-alpha} decline of the emitting dust, with alpha > 0.8.Comment: 11 pages, 3 figures, 3 tables; accepted for publication in Astronomy
& Astrophysic
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