(INFLUENCE OF Lactobacillus farciminis ON FERMENTATION IN LARGE INTESTINE AND PRODUCTION OF GASES ON IN VITRO IN HORSE)

The current study was outlined to investigate the influence of fecal inocula obtained from horses supplemented with Lactobacillus farciminis (L. farciminis) in diets constituting 50% oat straw on in vitro gas production (GP), methane (CH4), and carbon dioxide (CO2) productions as indicators of hindgut activity. The experiments were assessed on four Azteca horses ranging from 5 to 8 years of age, weighing 480±20.1 kg. The treatments comprised the in vitro incubation of fecal inocula with commercial product of L. farciminis at 0, 2, 4, and 6 mg/g dry matter (DM) of substrate. The fecal content mixed with the culture media were used to inoculate into the substrate containing a mixture of commercial concentrate and oat straw (1:1 DM). The subsequent incorporation of additives resulted into increased level of asymptotic GP, CH4, and CO2 productions (P0.05; quadric, P>0.05). A significant reduction in the fermentation pH range (linear, P=0.029) and higher metabolizable energy (ME) values (P=0.001) were obtained with the supplementation of L. farciminis in a dose dependent manner, whereas no impact of additives were reported on DMD (dry matter degraded) values (P>0.05). The in vitro GP, CH4, and CO2 productions were measured up to 48 h post incubation using standard methodology, and depicted 24 higher responses. The additives at varied concentrations resulted in increased in vitro GP, CH4, and CO2 productions (linear, P≤0.001) from 6 to 48 h of incubation. In conclusion, the dose dependent addition of L. farciminis (2 to 6 mg/g DM of diet) found to be persuasive in terms of attaining amicable hindgut fermentation in order to digest fibrous forage by horses without any side effect

Coxiella burnetii: Living inside the host cell

Coxiella burnetii is an obligate intracellular pathogen, causal agent of Q fever. In this brief review, we describe how recent described mechanisms help our understanding of C. burnetii invasion and his survival in the host cell by the formation of a replicative niche: the Coxiella-containing vacuole. We describe the actin-associated proteins involved in the internalization of C. burnetii, and we discuss the contribution of diverse degradative pathway of the cell during the formation and stabilization of the Coxiella-containing vacuole.Fil: Distel, Jesús Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Beron, Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Ortiz Flores, Rodolfo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentin

Estrategias de Regeneración Urbana Paisajística del Asentamiento Valle Verde y Valoración del Ecosistema de los Humedales de Ventanilla

Este trabajo estudia las estrategias de regeneración urbana paisajística que influyen en la conservación de los Humedales de Ventanilla. Para ello se describen los cambios y transformaciones urbanas que surgen alrededor del Humedal producto de las demandas de usos de suelo para viviendas. Así mismo se analiza el rol y la importancia de los ciudadanos en interactuar y valorar los servicios que le otorga este tipo de paisajes a la ciudad. Esta interacción es significativa porque constituyen una base de cambios y que definen la identidad local y participativa que integra a la sociedad. Para lograr el principal objetivo, la sostenibilidad y la conservación del paisaje natural en las Costas del Perú.Tesi

A visita pré-operatória de enfermagem em um hospital universitário durante a pandemia de SARS-CoV-2

The aim was to report the experience of nurses from a surgical center in carrying out the preoperative visit during the SARS-CoV-2 pandemic period. Descriptive study, experience report type, based on a preoperative visit at a university hospital in Rio de Janeiro, RJ. The preoperative patient who received a visit from the nurse is better informed about the surgical procedures and care related to the new coronavirus. The fact that most of them were not with a family member/companion, this moment brought a reassuring component to the patient, which facilitated the work of the nursing team in the trans and postoperative periods. The visits provide the operating room nurse with greater knowledge about the patient's clinical status, in addition to encouraging the multidisciplinary team to perform adequate perioperative care for safe surgery.Objetivou-se relatar a experiência de enfermeiras de um centro cirúrgico na realização da visita pré-operatória no período de pandemia de SARS-CoV-2. Estudo descritivo, tipo relato de experiência, baseado na realização de visita pré-operatória em um hospital universitário do Rio de Janeiro, RJ. O paciente pré-operatório que recebeu a visita da enfermeira é melhor informado quanto aos procedimentos cirúrgicos e os cuidados referentes ao novo coronavírus. Fato da maior parte não estar com familiar/acompanhante este momento trouxe um componente tranquilizador para o paciente, e que facilitou o trabalho da equipe de enfermagem do trans e pós-operatório. A realização das visitas propicia a enfermeira de centro cirúrgico um conhecimento maior sobre o estado clínico do paciente, além de fomentar na equipe multiprofissional a realização dos cuidados perioperatórios adequados para a cirurgia segura

A visita pré-operatória de enfermagem em um hospital universitário durante a pandemia de SARS-CoV-2

The aim was to report the experience of nurses from a surgical center in carrying out the preoperative visit during the SARS-CoV-2 pandemic period. Descriptive study, experience report type, based on a preoperative visit at a university hospital in Rio de Janeiro, RJ. The preoperative patient who received a visit from the nurse is better informed about the surgical procedures and care related to the new coronavirus. The fact that most of them were not with a family member/companion, this moment brought a reassuring component to the patient, which facilitated the work of the nursing team in the trans and postoperative periods. The visits provide the operating room nurse with greater knowledge about the patient's clinical status, in addition to encouraging the multidisciplinary team to perform adequate perioperative care for safe surgery.Objetivou-se relatar a experiência de enfermeiras de um centro cirúrgico na realização da visita pré-operatória no período de pandemia de SARS-CoV-2. Estudo descritivo, tipo relato de experiência, baseado na realização de visita pré-operatória em um hospital universitário do Rio de Janeiro, RJ. O paciente pré-operatório que recebeu a visita da enfermeira é melhor informado quanto aos procedimentos cirúrgicos e os cuidados referentes ao novo coronavírus. Fato da maior parte não estar com familiar/acompanhante este momento trouxe um componente tranquilizador para o paciente, e que facilitou o trabalho da equipe de enfermagem do trans e pós-operatório. A realização das visitas propicia a enfermeira de centro cirúrgico um conhecimento maior sobre o estado clínico do paciente, além de fomentar na equipe multiprofissional a realização dos cuidados perioperatórios adequados para a cirurgia segura

The role of microtubules and the dynein/ dynactin motor complex of host cells in the biogenesis of the Coxiella burnetii-containing vacuole

Microtubules (Mts) are dynamic cytoskeleton structures that play a key role in vesicular transport. The Mts-mediated transport depends on motor proteins named kinesins and the dynein/dynactin motor complex. The Rab7 adapter protein FYCO1 controls the anterograde transport of the endocytic compartments through the interaction with the kinesin KIF5. Rab7 and its partner RILP induce the recruitment of dynein/dynactin to late endosomes regulating its retrograde transport to the perinuclear area to fuse with lysosomes. The late endosomal-lysosomal fusion is regulated by the HOPS complex through its interaction with RILP and the GTPase Arl8. Coxiella burnetii (Cb), the causative agent of Q fever, is an obligate intracellular pathogen, which generates a large compartment with autophagolysosomal characteristics named Cb-containing vacuole (CCV). The CCV forms through homotypic fusion between small non-replicative CCVs (nrCCV) and through heterotypic fusion with other compartments, such as endosomes and lysosomes. In this work, we characterise the role of Mts, motor proteins, RILP/Rab7 and Arl8 on the CCV biogenesis. The formation of the CCV was affected when either the dynamics and/or the acetylation state of Mts were modified. Similarly, the overexpression of the dynactin subunit non-functional mutants p150 Glued and RILP led to the formation of small nrCCVs. This phenomenon is not observed in cells overexpressing WT proteins, the motor KIF5 or its interacting protein FYCO1. The formation of the CCV was normal in infected cells that overexpressed Arl8 alone or together with hVps41 (a HOPS subunit) or in cells co-overexpressing hVps41 and RILP. The dominant negative mutant of Arl8 and the non-functional hVps41 inhibited the formation of the CCV. When the formation of CCV was affected, the bacterial multiplication diminished. Our results suggest that nrCCVs recruit the molecular machinery that regulate the Mts-dependent retrograde transport, Rab7/RILP and the dynein/dynactin system, as well as the tethering processes such as HOPS complex and Arl8 to finally originate the CCV where C. burnetii multiplies.Fil: Ortiz Flores, Rodolfo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Distel, Jesús Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Aguilera, Milton Osmar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Beron, Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentin

Discovery of an activity cycle in the solar-analog HD 45184. Exploring Balmer and metallic lines as activity proxy candidates

Context. Most stellar activity cycles similar to that found in the Sun have been detected by using the chromospheric Ca ii H&K lines as stellar activity proxies. However, it is unclear whether such activity cycles can be identified using other optical lines. Aims. We aim to detect activity cycles in solar-analog stars and determine whether they can be identified through other optical lines, such as Fe II and Balmer lines. We study the solar-analog star HD 45184 using HARPS spectra. The temporal coverage and high quality of the spectra allow us to detect both long- and short-term activity variations. Methods. We analysed the activity signatures of HD 45184 by using 291 HARPS spectra obtained between 2003 and 2014. To search for line-core flux variations, we focused on Ca ii H&K and Balmer Hα and Hβ lines, which are typically used as optical chromospheric activity indicators. We calculated the HARPS-S index from Ca ii H&K lines and converted it into the Mount Wilson scale. In addition, we also considered the equivalent widths of Balmer lines as activity indicators. Moreover, we analysed the possible variability of Fe ii and other metallic lines in the optical spectra. The spectral variations were analysed for periodicity using the Lomb-Scargle periodogram. Results. We report for the first time a long-term 5.14-yr activity cycle in the solar-analog star HD 45184 derived from Mount Wilson S index. This makes HD 45184 one of most similar stars to the Sun with a known activity cycle. The variation is also evident in the first lines of the Balmer series, which do not always show a correlation with activity in solar-type stars. Notably, unlike the solar case, we also found that the equivalent widths of the high photospheric Fe ii lines (4924 Å, 5018 Å and 5169 Å) are modulated (±2 mÅ) by the chromospheric cycle of the star. These metallic lines show variations above 4σ in the rms spectrum, while some Ba ii and Ti ii lines present variations at 3σ level, which could be considered as marginal variations. From short-term modulation of the S index we calculate a rotational period of 19.98 days, which agrees with its mean chromospheric activity level. We also clearly show that the activity cycles of HD 45184 can be detected in both Fe ii and Balmer lines.Fil: Flores Trivigno, Matias Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; ArgentinaFil: Gonzalez, Jorge Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; ArgentinaFil: Jaque Arancibia, Marcelo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; ArgentinaFil: Buccino, Andrea Paola. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Saffe, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; Argentin

ESTUDIO COMPARATIVO DEL DESARROLLO DE LA ALFABETIZACIÓN EN POBLACIONES DE EDUCACIÓN PRIMARIA Y PREESCOLAR

A comparative study about early literacy of 33 children from elementary school and 27 children from kindergarden was carried out, applying the Instrumento de Observación de los Logros de la Lectoescritura Inicial (Escamilla, Andrade, Basurto y Ruíz, 1996). The purpose of the present work was to characterize the level of early literacy considering the knowledge of the language written in both groups. The groups were evaluated in the first two weeks of the month of September and of October of the school cycle 2011-2012. In the results it was observed that they were only significant differences in the identification of letters. The results are discussed in relation to the concept of early literacy

Reconstruction and characterization of full-length begomovirus and alphasatellite genomes infecting pepper through metagenomics

In northwestern Argentina (NWA), pepper crops are threatened by the emergence of begomoviruses due to the spread of its vector, Bemisia tabaci (Gennadius). The genus Begomovirus includes pathogens that can have a monopartite or bipartite genome and are occasionally associated with sub-viral particles called satellites. This study characterized the diversity of begomovirus and alphasatellite species infecting pepper in NWA using a metagenomic approach. Using RCA-NGS (rolling circle amplification-next generation sequencing), 19 full-length begomovirus genomes (DNA-A and DNA-B) and one alphasatellite were assembled. This ecogenomic approach revealed six begomoviruses in single infections: soybean blistering mosaic virus (SbBMV), tomato yellow spot virus (ToYSV), tomato yellow vein streak virus (ToYVSV), tomato dwarf leaf virus (ToDfLV), sida golden mosaic Brazil virus (SiGMBRV), and a new proposed species, named pepper blistering leaf virus (PepBLV). SbBMV was the most frequently detected species, followed by ToYSV. Moreover, a new alphasatellite associated with ToYSV, named tomato yellow spot alphasatellite 2 (ToYSA-2), was reported for the first time in Argentina. For the Americas, this was the first report of an alphasatellite found in a crop (pepper) and in a weed (Leonurus japonicus). We also detected intra-species and inter-species recombinationInstituto de VirologíaFil: Bornancini, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; ArgentinaFil: Vaghi Medina, Carlos Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Lopez Lambertini, Paola Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentin

Lepidium meyenii (maca) and soy isoflavones reduce cardiac stunning of ischemia-reperfusion in rats by mitochondrial mechanisms

Background and aim: Phytoestrogens are traditionally used for cardiovascular risks but direct effects on the ischemic heart remain unclear. Plants with phytoestrogens are used for reducing menopausic symptoms and they could also be cardioprotectives. Here we investigated whether maca (Lepidium meyenii) contains isoflavones and prevents cardiac stunning, in comparison to soy isoflavones. Experimental procedure: Both products were orally and daily administered to rats during 1 week before exposing isolated hearts to ischemia/reperfusion (I/R). Young male (YM), female (YF) and aged female (AgF) rats treated with maca (MACA, 1 g/kg/day) or soy isoflavones (ISOF, 100 mg/kg/day) were compared to acute daidzein (DAZ, 5 mg/kg i.p.) and non-treated rat groups. Isolated ventricles were perfused inside a calorimeter to simultaneously measure contractile and calorimetrical signals before and during I/R. Results and conclusions: Maca has genistein and daidzein. MACA and ISOF improved the post-ischemic contractile recovery (PICR) and muscle economy (P/Ht) in YM and YF hearts, but not in AgF hearts. DAZ improved PICR and P/Ht more in YM than in YF. The mKATP channels blockade reduced both PICR and P/Ht in DAZ-treated YM hearts, without affecting them in ISOF or MACA-treated YM hearts. In MACA treated YF hearts, the simultaneous blockade of NOS and mKATP channels, or the mNCX blockade reduced cardioprotection. Results show that subacute oral treatment with maca or with soy isoflavones was strongly preventive of cardiac ischemic dysfunction, more than the acute administration of a pure isoflavone (daidzein, genistein). Maca induced synergistic and complex mechanisms which prevented mitochondrial calcium overload.Fil: Colareda, German Andres. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Matera, Soledad Inés. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Bayley, Matias. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Ragone, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; ArgentinaFil: Flores, Maria Lujan. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales - Sede Comodoro. Departamento de Farmacia. Cátedra de Farmacognosia; ArgentinaFil: Córdoba, Osvaldo León. Universidad Nacional de la Patagonia "San Juan Bosco". Facultad de Ciencias Naturales y Ciencias de la Salud - Sede Esquel. Departamento de Química; ArgentinaFil: Consolini, Alicia Elvira. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Cátedra de Farmacología; Argentin