Recent changes on the abundance and distribution of non-indigenous macroalgae along the southwest coast of the Bay of Biscay

Twenty-three rocky shores along approximately 225 km on the southwest coast of the Bay of Biscay were sampled during the springs of 2014 and 2021, to explore changes in the distribution and abundance of four non-indigenous species (NIS) macroalgae (i.e., Asparagopsis armata, Grateloupia turuturu, Sargassum muticum, and Undaria pinnatifida) by using a semi-quantitative scale. Results showed relevant changes in the distribution and abundance of NIS. The kelp U. pinnatifida was recorded in 2021 for the first time on two shores. The distribution of G. turuturu showed an extension in its range of distribution of 200 km to the east. The other two target species S. muticum and A. armata were widely distributed along the whole 225 km of the studied area in 2014 and 2021, with higher abundance in 2021. Therefore, we strongly advise the necessity of future monitoring programs for these four NIS species. These monitoring programs will explore the progress of invasion and resilience of native species.This study was supported by the project ATLANTIDA (ref. NORTE-01-0145-FEDER-000040), supported by the Norte Portugal Regional Operational Program (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement and through the European Regional Development Fund (ERDF). This study was supported by the FCT-Foundation for Science and Technology through national funds within the scope of Contrato-Programa” UIDB/04050/2020 funded by national funds through the FCT I.P and, UIDB/04423/2020, UIDP/04423/2020. L. Guerrero-Meseguer was financed thanks to a grant from the Department of European Funds, University, and Culture of the Government of the Balearic Islands. P. Veiga was hired through the Regulamento do Emprego Científico e Tecnológico-RJEC from the Portuguese Foundation for Science and Technology (FCT) program (CEECIND/03893/2018)

Artificial substrates as sampling devices for marine epibenthic fauna: A quest for standardization

In temperate regions, macroalgae are naturally abundant habitat builders for many organisms of ecological and economic importance. Hence, macroalgae are good targets for monitoring studies based on colonization processes as, through them, it is possible to sample the epifauna that uses them as habitat. Nevertheless, macroalgae collection may not be sustainable, can compromise the survival of the target macroalgae populations and destroy fragile or threatened communities.The search for an adequate procedure that can overcome the problems related to destructive quantitative sampling of the epifauna associated with macroalgae and the development of a methodology that can be used for comparative macrofauna monitoring, regardless of the location, were the motivations for this study. The evaluation of the mobile epifauna associated with Artificial Substrates (AS) with different degrees of complexity and natural subtidal macroalgae was implemented, as a means to evaluate the viability of AS as an alternative approach for epifauna monitoring.Cystoseira baccata and Halidrys siliquosa were chosen as natural macroalgae. The hypotheses tested were (1) macroalgae and AS with similar structure will support similar assemblages of mobile epifauna; (2) different complexity AS will shelter different assemblages of mobile epifauna. The results obtained after 3 and 6 months showed that AS and macroalgae, both with similar structure, supported different assemblages of mobile epifauna, differing also when the time factor is considered. Moreover, different complexity AS supported different epifaunal assemblages. Our results also show that a period of colonization of 3 months was enough to accurately discriminate locations but the 3 additional months reinforce these differences and provide more coherent results with the species colonizing natural macroalgae in both locations studied. Hence, AS could be used as a valid, replicable, standard and representative alternative tool for monitoring studies.The present study was cofinanced by NextSea: Next generation monitoring of coastal ecosystems in a scenario of global change (operation NORTE-01-0145-FEDER-000032) and by FSE: Fundo Social Europeu, Portugal through NORTE 2020. Diego CarreiraFlores was supported by a PhD Fellowship (BD/Do*Mar/1010/2016) do Programa de Ciencia, Tecnologia e Gestao Marinha (Do*Mar), Portugal, operation number NORTE-08-5369-FSE-000039. This work was also supported by the strategic programme UID/BIA/04050/2019 funded by national funds through the FCT I.P, Portugal

Assessing the seasonal and spatial dynamics of zooplankton through DNA metabarcoding in a temperate estuary

Zooplankton are key components of estuarine trophic networks. However, routine monitoring is hindered by the difficulty of morphology-based identification. DNA-based methods allow us to circumvent some of these hurdles, providing precise species identifications regardless of the taxonomic expertise of the investigator or the developmental stage of the specimens. However, the process is dependent on the completeness of the reference libraries. In this study, we sought to evaluate the potential of DNA metabarcoding to assess the seasonal (summer, autumn, and early spring) and spatial dynamics of zooplankton (four locations spanning ca. 6 km) in the Lima estuary (NW Portugal). Two genetic markers were used: the cytochrome c oxidase subunit I and the V4 hypervariable region of the ribosomal 18S rRNA genes. Overall, 327 species were recovered, and both markers displayed minute overlap (7% were detected with both markers). Species richness, composition, and taxonomic distinctness were majorly influenced by the season, with a declining tendency from summer (highest number of exclusive species, n = 74) to spring. Second to season, the taxa composition was influenced by spatial variation where the most downstream site displayed the highest number of exclusive species, n = 53. A total of 16 non-indigenous species were detected using metabarcoding, but only one (Austrominus modestus) has been documented out in the estuary. In conclusion, both the seasonal and spatial gradients influenced the recovered richness, composition, and taxonomic distinctness, confirming the great aptitude of DNA metabarcoding for providing higher density monitoring and shedding new light on the composition and dynamics of complex zooplankton communities.FCT -Fundação para a Ciência e a Tecnologia(undefined

A marine biodiversity observation network for genetic monitoring of hard-bottom communities (ARMS-MBON)

Marine hard-bottom communities are undergoing severe change under the influence of multiple drivers, notably climate change, extraction of natural resources, pollution and eutrophication, habitat degradation, and invasive species. Monitoring marine biodiversity in such habitats is, however, challenging as it typically involves expensive, non-standardized, and often destructive sampling methods that limit its scalability. Differences in monitoring approaches furthermore hinders inter-comparison among monitoring programs. Here, we announce a Marine Biodiversity Observation Network (MBON) consisting of Autonomous Reef Monitoring Structures (ARMS) with the aim to assess the status and changes in benthic fauna with genomic-based methods, notably DNA metabarcoding, in combination with image-based identifications. This article presents the results of a 30-month pilot phase in which we established an operational and geographically expansive ARMS-MBON. The network currently consists of 20 observatories distributed across European coastal waters and the polar regions, in which 134 ARMS have been deployed to date. Sampling takes place annually, either as short-term deployments during the summer or as long-term deployments starting in spring. The pilot phase was used to establish a common set of standards for field sampling, genetic analysis, data management, and legal compliance, which are presented here. We also tested the potential of ARMS for combining genetic and image-based identification methods in comparative studies of benthic diversity, as well as for detecting non-indigenous species. Results show that ARMS are suitable for monitoring hard-bottom environments as they provide genetic data that can be continuously enriched, re-analyzed, and integrated with conventional data to document benthic community composition and detect non-indigenous species. Finally, we provide guidelines to expand the network and present a sustainability plan as part of the European Marine Biological Resource Centre (www.embrc.eu).Peer reviewe

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Review of populations of Zostera in Ría de Ribadeo. Legal framework, challenges and possibilities for the future

Neste traballo faise unha relación das cinco especies de plantas vasculares mariñas presentes na Comunidade Autónoma de Galicia (Zostera marina, Zostera noltii, Ruppia maritima, Najas minor, Najas marina) cos tipos de Unidades Ambientais do Plan Director da Rede Natura 2.000 de Galicia e os tipos de hábitats da Directiva 92/43/CEE, facendo especial fincapé nas dúas especies de Zostera por estar protexidas por distintos catálogos de protección estatal e autonómico. Posteriormente faise unha revisión bibliográfica sobre as dúas especies de Zostera na Ría de Ribadeo e elabórase unha novo mapa de presenza das dúas especies en cuadrículas UTM de 125 x 125 m. Finalmente, avalíase o estado de conservación de ámbalas dúas especies de acordo coa clasificación dos informes da Axencia Europea de medio Ambiente sobre o Artigo 17 da DC92/43/CEE.This work establishes a relationship between the five species of marine seagrass present in Galicia (Zostera marina, Zostera noltii, Ruppia maritima, Najas minor, Najas marina) with the types of Environmental Units of the Plan Director da Rede Natura 2.000 de Galicia and the habitat types of the Council Directive 92/43 / ECC, emphasizing two Zostera species as they are protected by different national and regional protection protocols. Subsequently, a review of the two species of Zostera in the Ría de Ribadeo is carried out, and a new presence map of the two species is elaborated in UTM squares of 125 x 125 m. Finally, the conservation status of both species is evaluated according to the classification of the European Environment Agency reports on the Article 17 of DC92 / 43 / EEC

Exploring Benthic Scavenger Assemblages, a Multi-Habitat Approach in NW Iberian Peninsula

Scavenging fauna plays an integral role in ecosystem functioning, nutrient cycling and circulating organic matter. Thus, baseline data of scavenger assemblages on the NW coast of the Iberian Peninsula that provides information on the abundance of species in different habitats is crucial to understanding the distribution of this understudied group. Two different types of baited traps, to capture scavenging megafaunal and scavenging benthopelagic macrofaunal assemblages, were deployed to compare them among rocky, pebbled and sandy bottoms at shallow subtidal on the northern coast of Portugal. Results showed significant differences in the structure of scavenger assemblages. Scavenging megafaunal assemblages differed between sandy and the other two studied habitats, whereas benthopelagic macrofaunal assemblages differed between pebbles and rocky habitats. This suggests that different drivers seem to shape the structure of benthopelagic macrofauna and megafauna. Regarding megafauna, the dominance of Tritia gastropods in sand habitats in comparison with its absence in rock and its very low abundance in pebbles was mainly responsible for the differences. However, in benthopelagic macrofauna, differences in assemblages between pebbles and rock were more related to changes in the relative abundance of crustacean species (Cirolana cranchii and Socarnes erythrophthalmus) that were less abundant in pebbles

Colonization in Artificial Seaweed Substrates: Two Locations, One Year

Artificial substrates have been implemented to overcome the problems associated with quantitative sampling of marine epifaunal assemblages. These substrates provide artificial habitats that mimic natural habitat features, thereby standardizing the sampling effort and enabling direct comparisons among different sites and studies. This paper explores the potential of the “Artificial Seaweed Monitoring System” (ASMS) sampling methodology to evaluate the natural variability of assemblages along a coastline of more than 200 km, by describing the succession of the ASMS’ associated macrofauna at two Rías of the Galician Coast (NW Iberian Peninsula) after 3, 6, 9, and 12 months after deployment. The results show that macrofauna assemblages harbored by ASMS differ between locations for every type of data. The results also support the hypothesis that succession in benthic communities is not a linear process, but rather a mixture of different successional stages. The use of the ASMS is proved to be a successful standard monitoring methodology, as it is sensitive to scale-dependent patterns and captures the temporal variability of macrobenthic assemblages. Hence, the ASMS can serve as a replicable approach contributing to the “Good Environmental Status” assessment through non-destructive monitoring programs based on benthic marine macrofauna monitoring, capturing the variability in representative assemblages as long as sampling deployment periods are standard

The conservation status of the world’s reptiles

Effective and targeted conservation action requires detailed information about species, their distribution, systematics and ecology as well as the distribution of threat processes which affect them. Knowledge of reptilian diversity remains surprisingly disparate, and innovative means of gaining rapid insight into the status of reptiles are needed in order to highlight urgent conservation cases and inform environmental policy with appropriate biodiversity information in a timely manner. We present the first ever global analysis of extinction risk in reptiles, based on a random representative sample of 1500 species (16% of all currently known species). To our knowledge, our results provide the first analysis of the global conservation status and distribution patterns of reptiles and the threats affecting them, highlighting conservation priorities and knowledge gaps which need to be addressed urgently to ensure the continued survival of the world’s reptiles. Nearly one in five reptilian species are threatened with extinction, with another one in five species classed as Data Deficient. The proportion of threatened reptile species is highest in freshwater environments, tropical regions and on oceanic islands, while data deficiency was highest in tropical areas, such as Central Africa and Southeast Asia, and among fossorial reptiles. Our results emphasise the need for research attention to be focussed on tropical areas which are experiencing the most dramatic rates of habitat loss, on fossorial reptiles for which there is a chronic lack of data, and on certain taxa such as snakes for which extinction risk may currently be underestimated due to lack of population information. Conservation actions specifically need to mitigate the effects of human-induced habitat loss and harvesting, which are the predominant threats to reptiles

Worldwide Disparities in Recovery of Cardiac Testing 1 Year Into COVID-19

BACKGROUND The extent to which health care systems have adapted to the COVID-19 pandemic to provide necessary cardiac diagnostic services is unknown.OBJECTIVES The aim of this study was to determine the impact of the pandemic on cardiac testing practices, volumes and types of diagnostic services, and perceived psychological stress to health care providers worldwide.METHODS The International Atomic Energy Agency conducted a worldwide survey assessing alterations from baseline in cardiovascular diagnostic care at the pandemic's onset and 1 year later. Multivariable regression was used to determine factors associated with procedure volume recovery.RESULTS Surveys were submitted from 669 centers in 107 countries. Worldwide reduction in cardiac procedure volumes of 64% from March 2019 to April 2020 recovered by April 2021 in high- and upper middle-income countries (recovery rates of 108% and 99%) but remained depressed in lower middle- and low-income countries (46% and 30% recovery). Although stress testing was used 12% less frequently in 2021 than in 2019, coronary computed tomographic angiography was used 14% more, a trend also seen for other advanced cardiac imaging modalities (positron emission tomography and magnetic resonance; 22%-25% increases). Pandemic-related psychological stress was estimated to have affected nearly 40% of staff, impacting patient care at 78% of sites. In multivariable regression, only lower-income status and physicians' psychological stress were significant in predicting recovery of cardiac testing.CONCLUSIONS Cardiac diagnostic testing has yet to recover to prepandemic levels in lower-income countries. Worldwide, the decrease in standard stress testing is offset by greater use of advanced cardiac imaging modalities. Pandemic-related psychological stress among providers is widespread and associated with poor recovery of cardiac testing. (C) 2022 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation