Comparison between Airborne Pollen and Aeroallergen Quantification with the ChemVol Impact Sampler. Olive pollen vs Ole e 1

Comparison between Airborne Pollen and Aeroallergen Quantification with the ChemVol Impact Sampler. Olive pollen vs Ole e 1. Torres M.C.1, C. Antunes2, M.J. Velasco1, R. Ferro2, H. García-Mozo1, R. Ribeiro2, R.Brandao3, Galán, C1 and the HIALINE team4 1Department of Botany, Ecology and Plant Physiology, University of Córdoba 2Department of Chemistry, University of Évora, Portugal 3Department of Biology, University of Évora, Portugal 4J.T.M. Buters, Germany, M. Thibaudon, France, M. Smith, Great Britain, C. Galan, Spain, R. Brandao and C. Antunes, Portugal, G. Reese, Germany, R. Albertini, Italy, L. Grewling, Poland, A. Rantio-Lehtimäki, Finland, S. Jäger and U. Berger, Austria, I. Sauliene, Lithuania, L. Cecchi, Italy Nowadays, pollinosis is affecting a large percentage of population in the countries with a western life style. The existence of allergenic activity in the atmosphere is not only associated to pollen grains and fungal spores, but also to submicronic and paucimicronic biological particles. The origin of these allergens can be due to the rupture of pollen transported in the atmosphere or to the presence of allergens from other parts of the plant making amorphous material with an allergen load. Olive pollen is recognized as one of the main causes of allergic disease in the Mediterranean area. In this study we have tried to compare olive pollen count in the air and Ole e 1 as major allergen of this species, at two different localities in South of Europe: Evora (Portugal) and Córdoba (Spain). At each location both samplers were placed side-by-side. Pollen grains have been sampled using a volumetric Hirst type spore trap. Chemvol high-volume cascade impactor equipped with stages PM>10µm, 10 µm>PM>2.5µm were used for detecting aeroallergens. Ole e 1 major allergen was determined using allergen specific ELISA´s. Similar behaviour between pollen and the total allergenic load was observed during the pollen season. Nevertheless, at some occasions, during the previous and later period of the pollen season, airborne allergenic load was detected in South Spain, due to the contributions from other Oleaceae species. For this reason the use of these two different methodologies allow a better understanding of the allergenic load in the atmosphere. This work was supported in part by the European Agency for Health and Consumers EAHC, Luxembourg, under the grant agreement 20081107

Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in South Europe

The European project HIALINE: Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in South Europe C. Antunes1,2, R. Ferro2, R. Ribeiro2, Torres M.C.4, M.J. Velasco4H. García-Mozo4, Galán, C4 , R.Brandao1,3, M.Thibaudon5, R. Albertini6 Ugolotti. M.6, Usberti I.6, Dall’Aglio P.6 and the HIALINE team7 1DInstitute of Mediterranean Agricultural and Environmental Sciences –ICAAM, University of Évora, Portugal 2Department of Chemistry, University of Évora, Portugal 3Department of Biology, University of Évora, Portugal 4Department of Botany, Ecology and Plant Physiology, University of Córdoba 5Réseau National de l Surveillance Aerobiologique, Saint-Genis-l’Argentière, France 6Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma, Italy 7J.T.M. Buters, Germany, M. Thibaudon, France, M. Smith, Great Britain, C. Galan, Spain, R. Brandao and C.M. Antunes, Portugal, G. Reese, Germany, R. Albertini, Italy, L. Grewling, Poland, A. Rantio-Lehtimäki, Finland, S. Jäger and U. Berger, Austria, I. Sauliene, Lithuania, L. Cecchi, Italy Introduction: Nowadays, pollinosis is affecting a large percentage of population in countries with a western life style. The existence of allergenic activity in the atmosphere is not only associated to pollen grains and fungal spores, but also to submicronic and paucimicronic biological particles. The origin of these allergens can be due to the rupture of pollen transported in the atmosphere or to the presence of allergens from other parts of the plant making amorphous material with an allergen load. Poaceae pollen is recognized as one of the main causes of allergic disease in all Europe. In this study we have tried to compare Poaceae pollen counts in the air and Phl p 5, one of the major allergens of this family, through the use of a high-volume cascade impactor (Chemvol). This study was done in the frame of the European project HIALINE and it compares the results obtained in 2009 by 4 different partners participating in this project: in Córdoba (Spain), Évora (Portugal), Lyon (France) and Parma (Italy). Methodology: Pollen grains were sampled using a 7-day volumetric Hirst type spore trap. Chemvol high-volume cascade impactor equipped with stages PM>10µm, 10 µm>PM>2.5µm was used for detecting aeroallergens. In each stage polyurethane filters were use as an impacting substrate. Phl p 5 allergen was determined using an allergen specific ELISA. Antibodies for analysis were delivered by Allergopharma Joachim Ganzer KG, the industrial partner in this project. At each location both samplers were placed side-by-side. Results: Most of the allergen was collected in the PM>10µm fraction. Similar profiles between airborne pollen and the total allergenic load was observed during the pollen season. A good correlation was obtained between pollen count and allergen content of the air and a value of 2.5 pg/pollen grain of Poaceae was estimated. Discussion: This is the first year of this project. Nevertheless, results suggest that the allergenic load in outdoor air might be mainly due to pollen bursts. It supports the hypothesis that monitoring the allergens itself in ambient air might be an improvement in allergen exposure assessment. This work was supported in part by the European Agency for Health and Consumers EAHC, Luxembourg, under the grant agreement 2008110

Stochastic Multi-objective Optimisation of Exoskeleton Structures

In this study, a structural optimisation problem, addressed through a stochastic multi-objective approach, is formulated and solved. The problem deals with the optimal design of exoskeleton structures, conceived as vibration control systems under seismic loading. The exoskeleton structure is assumed to be coupled to an existing primary inner structure for seismic retrofit: the aim is to limit the dynamic response of the primary structure to prevent structural damage. A non-stationary filtered Gaussian white noise stochastic process is taken as the seismic input. Design variables pertain to the mechanical properties (stiffness, damping) of the exoskeleton structure. Two concurrent and competing objective functions are introduced, in order to take into account not only safety performance but also economic cost considerations. The resulting trade-off is solved searching the Pareto front by way of a controlled elitist genetic algorithm, derived from the Non-dominated Sorting Genetic Algorithm-II. Sensitivities of Pareto fronts and Pareto optimal sets to different system parameters are finally investigated by way of a numerical application

Relações entre stress parental, tempo dispensado aos filhos e ajuda nos TPC

Silva, A. R.; Mónico, L.; Nobre-Lima, L. & Ferro, M.J. (2015). Relações entre stress parental, tempo dispensado aos filhos e ajuda nos TPC. International Journal of Developmental and Educational Psychology, INFAD Revista de Psicologia, 1(1), 351-364A discussão académica acerca da questão dos trabalhos para casa (TPC) justificam a concretização do trabalho que agora aqui se apresenta. Neste artigo analisam-se as opiniões de pais e filhos face aos TPC e a associação dessas perspectivas com o stress parental. A amostra foi constituída por 50 crianças a frequentar o 2º ciclo do ensino público português e 50 pais. Utilizou-se um Questionário sociodemográfico e escolar para as crianças e adultos e a Escala de Stress Parental para pais. Neste estudo, a quase totalidade das crianças tem TPC diários, de dificuldade percebida entre “pouca” e “alguma”. Apesar de a maioria das crianças reconhecerem que os TPC contribuem para a obtenção de melhores notas, consideram que são em quantidade excessiva e que recorrem à ajuda dos progenitores para os realizar (sobretudo, recorrem ao apoio da mãe), ocupando assim cerca de uma hora diária. A maioria dos pais considera os TPC úteis na aprendizagem dos educandos, embora concordem que retiram tempo para a comunicação e interação familiares e podem ser fonte de conflito. Consideram que o volume de TPC é inadequado e reconhecem que os filhos não gostam de os fazer sendo que apenas ajudam quando para isso são solicitados. A opinião de que a aprendizagem deve ser feita exclusivamente na escola acolheu suporte por mais de metade dos pais. Registámos, em termos médios, níveis baixos de stress parental, embora mais elevados nos pais das crianças que têm TPC diários. Os pais que nunca ajudam a fazer os TPC apresentam menos preocupações parentais mas, por outro lado, a satisfação é maior e os medos e angústias menores nos pais que ajudam sempre os filhos. O stress parental foi menor em pais que dispensam mais tempo aos seus filhos, sobretudo pelas pontuações mais baixas na Falta de Controlo e Medos e Angústias

The European project HIALINE: Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in SW Europe

The European project HIALINE: Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in SW Europe C. Antunes1*, R. Ferro1, R. Ribeiro1, Torres M.C.3, M.J. Velasco3H. García-Mozo3, Galán, C3 , R.Brandao2, M.Thibaudon4 and the HIALINE team5 1Department of Chemistry, University of Évora, Portugal 2Department of Biology, University of Évora, Portugal 3Department of Botany, Ecology and Plant Physiology, University of Córdoba 4Réseau National de l Surveillance Aerobiologique, Saint-Genis-l’Argentière, France 5J.Buters, Germanny, M. Thibaudon, France, M. Smith, Great Britain, C. Galan, Spain, R. Brandao and C.M. Antunes, Portugal, R. Albertini, Italy, L. Grewling, Poland, A. Rantio-Lehtimäki, Finland, S. Jäger and U. Berger, Austria, I. Sauliene, Lithuania, L. Cecchi, Italy * Presenting Author: Antunes, C. Department of Chemistry, University of Évora, Portugal. Phone +351 266745319 ; email: [email protected] Introduction: Nowadays, pollinosis is affecting in a large percentage of population in the developed countries. The existence of allergenic activity in the atmosphere is not only associated to pollen grains and fungal spores, but also to submicronic and paucimicronic biological particles. The origin of these allergens can be due to the rupture of pollen transported in the atmosphere or to the presence of allergens from other parts of the plant making amorphous material with allergenic load. Poaceae pollen is recognized as one of the main causes of allergic disease in all Europe. In this study we have tried to compare Poaceae pollen counts in the air and Phl p 5, one of the major allergens of this family, through the use of a high-volume cascade impactor (Chemvol). This study was done in the frame of the European project HIALINE and it compares the results obtained in 2009 by 3 different partners participating in this project: in Córdoba (Spain), Évora (Portugal) and Lyon (France). Methodology: Pollen grains have been taken out using a 7-day volumetric Hirst type spore trap. Chemvol high-volume cascade impactor equipped with stages PM>10µm, 10 µm>PM>2.5µm were used for detecting aeroallergens. In each stage polyurethane filters were applied. Phl p5 allergen was determined using allergen specific ELISA´s. Antibodies for analysis are delivered by Allergopharma Joachim Ganzer KG, the industrial partner in this project. Both samplers were placed side-by-side. Results: Most of the allergen was collected in the PM>10µm fraction. Similar profiles between airborne pollen and the total allergenic load was observed during the pollen season. A good correlation was obtained and a value of 2.5 pg/pollen grain of Poaceae was estimated. Discussion: These results suggest that the allergenic load in outdoor air might be mainly due to pollen bursts. It supports the hypothesis that monitoring the allergens itself in ambient air might be an improvement in allergen exposure assessment. This work was supported in part by the European Agency for Health and Consumers EAHC, Luxembourg, under the grant agreement 2008110

Procura da boa norma para a localização industrial

Mestrado em Planeamento do Território - Ordenamento da CidadeO processo de localização industrial foi influenciado ao longo da História, por diversos factores, que ditaram a sua implantação e organização no território. Se, numa etapa inicial, estes factores dependiam, sobretudo, das necessidades físicas da produção, com a crescente complexidade e evolução tecnológica, registadas no último século, impõem-se, cada vez mais, os de natureza intangível, relacionados com as formas de acesso à informação, o conhecimento, as externalidades, entre outros. No processo de tomada de decisão de localização são identificados, por diversos autores, factores de localização industrial emergentes, que decorrem do conjunto de características inerentes ao local e meio envolvente. Conjuntamente com as características biofísicas, funcionais e fundiárias do local de implantação, são ponderados outros factores, com abrangências territoriais distintas. O desafio, que actualmente, se coloca ao ordenamento do território, de articulação das diferentes escalas de intervenção, aponta para a necessidade de utilizar metodologias mais integradoras, que identifiquem, por um lado, as existências, contextualizadas num referencial histórico e institucional, e por outro lado, as dinâmicas de localização industrial, sobretudo, através dos seus principais agentes. Esta investigação visa, precisamente, contribuir em termos metodológicos, para a aferição de tipologias de localização industrial, tendo por base, a realidade territorial do concelho da Batalha, como caso de estudo. Do cruzamento entre as abordagens teórica e prática, resulta uma proposta de modelo de intervenção, que se espera, que possa servir de guia na procura da boa norma para a localização industrial. ABSTRACT: Along History, the process of industrial location was influenced by several factors, which dictated its settlement and organization in the territory. If, in an initial stage, these factors were depended, especially, on the physical necessities of the production, with the growing complexity and technological evolution registered in the last century, there are imposed, more and more, those of intangible nature, related to means of information access, the knowledge, the externalities, and others. In the process of making decisions on location, several authors identify emergent factors of industrial location, which result from the set of inherent characteristics of the place and surrounding setting. Together with the biophysical environment, functional and land policy characteristics of the industrial settlement, other factors are considered, with different territorial ranges. The challenge, which at present is put to territorial management strategies of articulation of the different scales of intervention, points to the necessity of applying more integrated methodologies, which identify, on one hand, the existences, contextualized in an historical and institutional reference system, and on the other hand, the dynamics of industrial location, especially, through its main agents. Hence, this investigation aims to be a methodological tool for the determination of industrial location typologies, having for support the territorial reality of the municipality of Batalha, as case study. From the crosses between theoretical and practical approaches, results a proposal of an intervention model that is expected to serve as guide in the search of a good industrial location criterion

Futebol feminino na televisão portuguesa : a (in)visibilidade das mulheres

A televisão é o meio preferido dos portugueses e, por isso, assume um papel determinante enquanto instituição social. Permitindo a construção de interpretações e reproduções da realidade que são partilhadas na sociedade, a televisão configura­se e é vista como um forte alicerce da esfera social. Dado o seu posicionamento, a televisão possibilitou a expansão do desporto, nomeadamente do futebol, tendo­se tornado crucial no seu sucesso e dimensão à escala mundial. A evolução histórica tem demonstrado a (ínfima) presença das mulheres no futebol, ao mesmo tempo que a modalidade foi construída mediante uma hegemonia masculina. Deste modo, esta visibilidade conferida pela televisão ao futebol só se aplica aos homens, porque quando nos referimos ao futebol feminino o panorama é muito distinto, visto que no meio mediático a modalidade é centrada, sobretudo, no futebol masculino. A presente investigação visa cruzar os estudos que têm vindo a ser desenvolvidos nos últimos anos com uma reflexão e abordagem à posição que o futebol feminino apresenta hoje na sociedade, mas sobretudo, no meio mediático. Neste sentido, a investigação pretende analisar a representação do futebol feminino na televisão portuguesa, qualitativa e quantitativamente, com o intuito de averiguar se existe (in)visibilidade do desporto feminino em comparação com o desporto masculino. De modo a atingir os objetivos propostos na investigação, desenhou­se uma metodologia mista que engloba aferir a existência de aspetos textuais que desvalorizem os jogos femininos, conhecer as perspetivas dos profissionais que estão envolvidos no meio, mas também de quem assiste à modalidade (entrevistámos jornalistas desportivos, atletas de futebol feminino e inquirimos telespetadores) e, por último, realizámos uma análise que permitiu compreender a diferença que existe entre a presença televisiva do futebol feminino e masculino. Uma das principais conclusões a que chegámos é que o público não adere ao futebol feminino porque é algo cultural e ideológico e que leva a maioria das pessoas a demonstrar preferência pelo futebol masculino e a televisão não disponibiliza tanto tempo e espaço ao futebol feminino porque este não é impulsionador de audiência. Sumariamente, o público não adere ao futebol feminino porque não tem interesse e a televisão não lhe dá futebol feminino porque este não adere, ou seja, não adere porque não lhe dá, não lhe dá porque não adere.Television is the preferred medium of Portuguese people which assumes a decisive role as a social institution. Allowing the construction of interpretations and reproductions of reality that are shared in society, television is considered and seen as a strong foundation of the social sphere. Given its positioning, television allowed the possibility to expand sport, in particular football, which was crucial to its success and global dimension. Historical evolution has demonstrated the (tiny) presence of women in football, while the sport was built through male hegemony. Thus, this visibility given by television to football is only applied to men, because when we refer to women’s football the panorama is very different, since in the media the the modality is mainly focused on men's football. This research aims to cross­reference the studies that have been developed in the last years with a reflection and approach to the position that women’s football presents today in society, but above all, in the media. In this sense, the research aims to analyse the representation of women’s football in portuguese television, qualitatively and quantitatively, with the intention of searching whether there is (in)visibility of women’ sport compared to men’s sport. In order to achieve the proposed goals in the research, a mixed methodology was designed to assess the existence of textual aspects which diminish women’s games, to know the professionals’ perspectives that are involved in that sphere, but also of those who attend the sport (we interviewed sports journalists, women's football athletes and we surveyed viewers) and, finally, we carried out an analysis that allowed us to understand the difference between the television presence of women's and men's football. One of the main conclusions we reached is that the public does not adhere to women's football because it is something cultural and ideological and that leads most people to show preference for men’s football and television does not provide as much time and space to women’s football because this is not an audience booster. In short, the public does not adhere to women's football because they are not interested and television does not give them women's football because it does not adhere to it, that is, it does not adhere to it because it does not give it to it, it does not give it to it because it does not adhere to it

Sífilis: prevalência num Hospital de Lisboa

Copyright © Ordem dos MédicosIntroduction: Syphilis is a sexual and vertical transmitted disease. Its incidence is increasing in Europe, particularly, in Portugal. Material and methods: A descriptive, retrospective study was performed based on positive treponemal tests from January to December 2013, at the Santa Maria Hospital, Lisbon. In-patients and out-patients evaluated in medical appointments and at the emergency department were included. We proceeded to epidemiological characterization, disease classification and definition of risk factors. Results: We obtained a sample of 580 patients, of whom 51 with no clinical data and 45 with false positive serologies were excluded. There was a predominance of male patients (75%) and a mean age of 47 years. Most (59%) had syphilis successfully treated in the past and 3.7% were in follow-up. We recorded 13 primaries syphilis, 71 cases of secondary syphilis, 40 cases of early latent syphilis, 49 unknown duration syphilis and five cases of late latent syphilis. In the early syphilis group, 42% (n = 124) were HIV-positive and, in 8% both diagnosis were done simultaneously. Discussion: We emphasize the high prevalence of syphilis/HIV co-infection in patients with early syphilis, reinforcing the importance of promoting the use of preventive measures. We obtained 11% of patients with late clinical forms, which are notifiable since June 2014, in Portugal. All serological tests for the diagnosis of syphilis have limitations which emphasizes the importance of clinical-laboratory correlation. Conclusion: Syphilis remains an important public health problem. It is necessary to establish education programs, screening and follow-up strategies to reduce their prevalence and to perform more efficient screening of the partners.info:eu-repo/semantics/publishedVersio

Dry Dosage Forms of Add-Value Bioactive Phenolic Compounds by Supercritical CO2-Assisted Spray-Drying

PTDC/BII-BIO/30884/2017—POCI-01-0145-FEDER-030884 PD/00184/2012-PDQSEvery year, grapevine pruning produces huge amounts of residue, 90% of which are from vine shoots. These are a rich source of natural antioxidants, mostly phenolic compounds, which, when properly extracted, can give rise to added-value products. However, their lack of solubility in aqueous media and high susceptibility to thermal and oxidative degradation highly limit their bioavailability. Encapsulation in suitable carriers may have a positive impact on their bioavailability and bioactivity. Previous data on vine-shoot extraction have identified gallic acid (GA) and resveratrol (RSV) as the main phenolic compounds. In this work, model dry powder formulations (DPFs) of GA and RSV using hydroxypropyl cellulose (HPC) as carriers were developed using Supercritical CO2-Assisted Spray Drying (SASD). A 32 full factorial Design of Experiments investigated the solid and ethanol contents to ascertain process yield, particle size, span, and encapsulation efficiency. Amorphous powder yields above 60%, and encapsulation efficiencies up to 100% were achieved, representing excellent performances. SASD has proven to be an efficient encapsulation technique for these phenolic compounds, preserving their antioxidation potential after three months in storage with average EC50 values of 30.6 µg/mL for GA–DPFs and 149.4 µg/mL for RSV–DPF as assessed by the scavenging capacity of the DPPH radical.publishersversionpublishe