Microuidics control the ballistic energy of thermocavitation liquid jets for needle-free injections

Illuminating a water solution with a focused continuous wave laser produces a strong local heating of the liquid that leads to the nucleation of bubbles, also known as thermocavitation. During the growth of the bubble, the surrounding liquid is expelled from the constraining microfluidic channel through a nozzle, creating a jet. The characteristics of the resulting liquid jet was imaged using ultra-fast imaging techniques. Here, we provide a phenomenological description of the jet shapes and velocities, and compare them with a Boundary Integral numerical model. We define the parameter regime, varying jet speed, taper geometry and liquid volume, for optimal printing, injection and spray applications. These results are important for the design of energy-efficient needle-free jet injectors based on microfluidic thermocavitation

Estado de salud oral y percepcion de calidad de vida en ninos de 12 anos, de area rural y urbana, que asisten a algunos establecimientos municipalizados de las comunas de Talca, Maule, Pelarco y San Clemente, 2008

117 p.INTRODUCCIÓN: La Salud Oral (SO) resulta esencial para una óptima calidad de vida (CV). La “Calidad de Vida relativa a la Salud Oral” (CVRSO), es posible medirla con instrumentos como el CPQ11-14 (Child Perceptions Questionnaire) desarrollado a nivel internacional. OBJETIVO: Determinar el estado de SO y percepción de CV en niños de 12 años, de área rural y urbana que asisten a establecimientos educacionales municipalizados de Talca, Maule, Pelarco y San Clemente, 2008. MATERIALES Y METODO: Estudio descriptivo, no probabilístico. Se realizó un examen clínico y cuestionario a niños de 12 años, 148 rurales y 137 urbanos. Se evaluó COPD, IHO y auto-percepción de SO, relacionándola con CVRSO obtenida mediante el CPQ11-14-ESP (adaptación al español). Se consultó sobre el acceso a atención odontológica. RESULTADOS: El COPD obtenido fue 3,42 (rural) y 2,97 (urbano). La prevalencia de caries fue 66,2% rural y 61,3% urbano. IHO promedio obtenido fue 1,67 y 1,28 respectivamente. La auto-percepción del estado de SO fue regular. Los puntajes obtenidos en el CPQ11-14-ESP fueron mayores para sexo femenino, NSE bajo, COPD ≥ 4, y niños con mala autopercepción de SO. Poco más de la mitad de los niños han acudido al odontólogo dentro del último año. CONCLUSIÓN: La muestra urbana presentó una mejor estado de salud oral y calidad de vida. EL CPQ11-14-ESP, se correlaciona con COPD y autopercepción de SO. Los niños rurales, sexo femenino y NSE bajo, reportaron mayor efecto en su calidad de vida

UV degradation of primary mirrors in outdoor exposure and accelerated aging

The objective of this work is to quantify de degradation originated by UV radiation and temperature in silvered-glass reflectors exposed in three locations with high solar irradiance and to replicate the degradation mechanisms observed in outdoors through accelerated aging tests

Lifetime prediction model of reflector materials for concentrating solar thermal energies in corrosive environments

Concentrated solar thermal technologies play an essential role in the energetic transition which is currently facing our society. The energy generation in this technology vastly depends on the optical behaviour of the reflector materials of the solar field. Corrosion of solar reflectors might be an issue in locations with high corrosive environments because an excessive corrosion of the solar mirror could be catastrophic for the profitability of the concentrated solar thermal plant. This research is focusing on modelling the durability of four different solar reflector materials exposed outdoors by accelerated aging tests. For this purpose, ten locations suitable for concentrating solar thermal applications were classified depending on their corrosive aggressiveness. Commercial, free-lead and low-cost reflectors samples were exposed in all the sites to determine the influence of the corrosion in its durability. Corrosion defects appeared in the solar reflectors during outdoor exposure were properly reproduced by CASS test. Novel lifetime prediction models were developed for all the solar reflectors depending on the corrosive aggressiveness of the place. Number and thickness of the paint coatings employed in the solar mirrors were identified as one of the most important parameters to improve the energy generation of a CSP plant in corrosive environments. A reduction of the capital invested in the solar mirror purchase is expected for sites with low corrosivity

Análisis de marcadores de salud en el trail running

Estudio cuasiexperimental pre-post cuyo objetivo es analizar el impacto agudo producido en el organismo durante una carrera de montaña en corredores aficionados, analizando marcadores sanguíneos y valorando la posible perdida de fuerza en las extremidades inferiores tras la prueba

Quantitative Lipid Profiling Reveals Major Differences between Liver Organoids with Normal Pi*M and Deficient Pi*Z Variants of Alpha-1-antitrypsin.

Different mutations in the SERPINA1 gene result in alpha-1 antitrypsin (AAT) deficiency and in an increased risk for the development of liver diseases. More than 90% of severe deficiency patients are homozygous for Z (Glu342Lys) mutation. This mutation causes Z-AAT polymerization and intrahepatic accumulation which can result in hepatic alterations leading to steatosis, fibrosis, cirrhosis, and/or hepatocarcinoma. We aimed to investigate lipid status in hepatocytes carrying Z and normal M alleles of the SERPINA1 gene. Hepatic organoids were developed to investigate lipid alterations. Lipid accumulation in HepG2 cells overexpressing Z-AAT, as well as in patient-derived hepatic organoids from Pi*MZ and Pi*ZZ individuals, was evaluated by Oil-Red staining in comparison to HepG2 cells expressing M-AAT and liver organoids from Pi*MM controls. Furthermore, mass spectrometry-based lipidomics analysis and transcriptomic profiling were assessed in Pi*MZ and Pi*ZZ organoids. HepG2 cells expressing Z-AAT and liver organoids from Pi*MZ and Pi*ZZ patients showed intracellular accumulation of AAT and high numbers of lipid droplets. These latter paralleled with augmented intrahepatic lipids, and in particular altered proportion of triglycerides, cholesterol esters, and cardiolipins. According to transcriptomic analysis, Pi*ZZ organoids possess many alterations in genes and cellular processes of lipid metabolism with a specific impact on the endoplasmic reticulum, mitochondria, and peroxisome dysfunction. Our data reveal a relationship between intrahepatic accumulation of Z-AAT and alterations in lipid homeostasis, which implies that liver organoids provide an excellent model to study liver diseases related to the mutation of the SERPINA1 gene.This research was funded by INSTITUTO DE SALUD CARLOS III (ISCIII), grants numbers AESI PI20CIII/00015 and PT20CIII/00009, and the APC was funded by AESI PI20CIII/00015.S

Direct-acting antiviral therapy improves kidney survival in hepatitis C virus-associated cryoglobulinaemia: the RENALCRYOGLOBULINEMIC study

Background: Direct-acting antiviral agents (DAAs) have shown high rates of sustained virological response in chronic hepatitis C virus (HCV) infection. However, the influence of DAAs on the course of kidney involvement in HCV-associated mixed cryoglobulinaemia (HCV-MC) has been little studied. The aim of this study was to analyse the effects of antiviral treatment on kidney prognosis and evolution in patients diagnosed with HCV-MC. Methods: The RENALCRYOGLOBULINEMIC study is an observational multicentre cohort study of 139 patients with HCV-MC from 14 Spanish centres. Clinical and laboratory parameters were measured before and after antiviral treatment. Primary endpoints were kidney survival and mortality after HCV-MC diagnosis. Secondary endpoints were clinical, immunological and virological responses after antiviral treatment. Results: Patients were divided into three groups based on the treatment received: treatment with DAAs (n = 100) treatment with interferon (IFN) and ribavirin (RBV) (n = 24) and no treatment (n = 15). Patients were followed up for a median duration of 138 months (interquartile range 70-251. DAA treatment reduced overall mortality {hazard ratio [HR] 0.12 [95% confidence interval (CI) 0.04-0.40]; P < 0.001} and improved kidney survival [HR 0.10 ( 95% CI 0.04-0.33); P < 0.001]. Conclusions: Results from the RENALCRYOGLOBULINEMIC study indicated that DAA treatment in patients with HCV-MC improves kidney survival and reduces mortality

Combining neutrophil and macrophage biomarkers to detect active disease in ANCA vasculitis: a combinatory model of calprotectin and urine CD163.

Background: CD163 and calprotectin have been proposed as biomarkers of active renal vasculitis. This study aimed to determine whether the combination of serum/urine calprotectin (s/uCalprotectin) and urinary soluble CD163 (suCD163) increases their individual performance as activity biomarkers. Methods: We included 138 patients diagnosed with ANCA vasculitis (n = 52 diagnostic phase, n = 86 remission). The study population was divided into the inception (n = 101) and the validation cohorts (n = 37). We determined the s/uCalprotectin and suCD163 concentration using enzyme-linked immunoassay at the diagnostic or at the remission phase. Receiver operating characteristic (ROC) curves were conducted to assess the biomarkers' classificatory values. We elaborated a combinatorial biomarker model in the inception cohort. The ideal cutoffs were used in the validation cohort to confirm the model's accuracy in the distinction between active disease and remission. We added the classical ANCA vasculitis activity biomarkers to the model to increase the classificatory performance. Results: The concentrations of sCalprotectin and suCD163 were higher in the diagnostic compared with the remission phase (P = .013 and P < .0001). According to the ROC curves, sCalprotectin and suCD163 were accurate biomarkers to discern activity [area under the curve 0.73 (0.59-0.86), P = .015 and 0.88 (0.79-0.97), P < .0001]. The combinatory model with the best performance in terms of sensitivity, specificity and likelihood ratio included sCalprotectin, suCD163 and haematuria. Regarding the inception and the validation cohort, we obtained a sensitivity, specificity and likelihood ratio of 97%, 90% and 9.7, and 78%, 94% and 13, respectively. Conclusions: In patients with ANCA vasculitis, a predictive model combining sCalprotectin, suCD163 and haematuria could be useful in detecting active kidney disease

Development of EULAR recommendations for the reporting of clinical trial extension studies in rheumatology

Objectives: Our initiative aimed to produce recommendations on post-randomised controlled trial (RCT) trial extension studies (TES) reporting using European League Against Rheumatism (EULAR) standard operating procedures in order to achieve more meaningful output and standardisation of reports. Methods: We formed a task force of 22 participants comprising RCT experts, clinical epidemiologists and patient representatives. A two-stage Delphi survey was conducted to discuss the domains of evaluation of a TES and definitions. A ‘0–10’ agreement scale assessed each domain and definition. The resulting set of recommendations was further refined and a final vote taken for task force acceptance. Results: Seven key domains and individual components were evaluated and led to agreed recommendations including definition of a TES (100% agreement), minimal data necessary (100% agreement), method of data analysis (agreement mean (SD) scores ranging between 7.9 (0.84) and 9.0 (2.16)) and reporting of results as well as ethical issues. Key recommendations included reporting of absolute numbers at each stage from the RCT to TES with reasons given for drop-out at each stage, and inclusion of a flowchart detailing change in numbers at each stage and focus (mean (SD) agreement 9.9 (0.36)). A final vote accepted the set of recommendations. Conclusions: This EULAR task force provides recommendations for implementation in future TES to ensure a standardised approach to reporting. Use of this document should provide the rheumatology community with a more accurate and meaningful output from future TES, enabling better understanding and more confident application in clinical practice towards improving patient outcomes