Vers des environnements virtuels plus écologiques : étude des modifications du comportement moteur en réalité virtuelle lors de l'ajout d'informations haptiques par un mécanisme parallèle entraîné par câbles

Introduction : Les nouvelles technologies qui permettent de capter et d’analyser les mouvements des utilisateurs ne cessent de se développer et représentent un potentiel intéressant dans le domaine de la santé. Grâce à l’essor de ces nouvelles technologies, des systèmes de réalité virtuelle (RV) clefs en main intègrent les services de réadaptation, et les études démontrent leur capacité à optimiser la rééducation motrice et l’évaluation des clients présentant des troubles du contrôle moteur. Le marché de la RV est ainsi en pleine expansion, et l’ajout d’informations haptiques permettant de modéliser les caractéristiques physiques des entités virtuelles représente un intérêt considérable pour améliorer l’écologie des environnements virtuels (EVs) et le transfert des apprentissages aux activités quotidiennes. Toutefois, l’effet de l’ajout de ces informations sur le comportement moteur des sujets demeure très peu connu. L’objectif principal de cette thèse était ainsi d’évaluer l’impact de l’ajout d’informations haptiques, par un mécanisme parallèle entrainé par câbles (robot à câbles), sur le contrôle moteur de sujets sains, lors de la réalisation de tâches complexes et fonctionnelles dans des EVs. Les deux hypothèses principales étaient que cet ajout améliore le contrôle du mouvement lors de tâche de manutention d’objet ayant des contraintes environnementales statiques, et modifie les stratégies locomotrices proactives en présence de contraintes dynamiques. Méthode : Le comportement moteur de participants sains a été analysé lors de la réalisation de deux tâches. En premier lieu, une tâche de manutention de caisse nécessitant la préhension et le déplacement d’une caisse à partir d’une posture debout a été étudiée. Celle-ci a été réalisée dans un environnement réel et dans des EVs, en absence et en présence d’informations haptiques, relatives aux contraintes physiques de l’étagère et de la caisse manipulée, fournies grâce à un robot à câbles (Chapitre 3, N=12). En second lieu, une tâche nécessitant l’évitement d’avatars au cours de la marche sur un tapis roulant a été réalisée en présence et en absence de risque de contact physique avec les avatars, délivré par un robot à câbles (Chapitre 4, N=10). Les EVs étaient vus au travers d’un visiocasque. Résultats : La première étude a démontré une amélioration des paramètres spatiaux du mouvement réalisé dans l’EV en présente d’informations haptiques, au cours des différentes phases de la tâche de manutention (préhension, montée et descente de la caisse). L’organisation spatiale du mouvement était ainsi plus similaire à ce qui était observé dans un environnement réel, avec un meilleur respect des contraintes environnementales (éloignement plus important de la caisse avec l’étagère, trajectoire plus longue). De plus, le contrôle du mouvement était influencé par la demande de précision requise pour ne pas toucher les étagères en présence d’informations haptiques uniquement. La deuxième étude a démontré la mise en place de stratégies motrices plus précautionneuses pour éviter les avatars lors de l’ajout d’informations haptiques. Les participants tendaient à anticiper plus précocement l’évitement des avatars. Ils maintenaient une distance minimale plus importante avec les avatars et conservaient un espace péripersonnel plus large, indépendamment de l’angle d’approche de l’avatar. Conclusion : L’ajout d’informations haptiques dans les EVs impacte les stratégies motrices proactives des participants sains aussi bien lors de la tâche de manutention de caisse que de locomotion avec évitement d’avatars. Les résultats suggèrent que l’ajout d’informations haptiques favorise la prise en compte des entités virtuelles lors de la planification mouvement. Ces informations haptiques imposent en effet des restrictions plus réalistes dans les possibilités d’actions fournies par les EVs, et modifient probablement l’évaluation des conséquences que représente le contact avec les entités virtuelles. Il serait pertinent de poursuivre l’étude de l’influence de ces informations afin de proposer à des clients ayant des déficiences motrices des environnements encore plus écologiques, qui favorisent l’évaluation et la prise en compte des risques implicites que représentent les entités environnementales.Introduction: New technologies that capture and analyze user movement are constantly developing and represent a great potential in healthcare. Thanks to the recent technological advances, turnkey virtual reality (VR) systems are progressively integrated into the rehabilitation setting, and studies have demonstrated their ability to optimise sensorimotor rehabilitation and clinical assessment of people with motor control disorders. The market for VR is growing and adding haptic feedback that provides physical characteristics to virtual entities represents a great potential to improve the ecological validity of virtual environments (VE) and to the transfer of learning to daily tasks. However, the impact that adding haptic feedback has on motor behavior remains poorly understood. The main objective of this thesis was to assess the impact of adding haptic feedback, using a novel cable-driven parallel robot, on the motor control of healthy participants during complex, functional tasks in VEs. The two mains hypotheses were that haptic feedback improves motor control during a handling task with static environmental constraints and modifies proactive locomotor strategies in the presence of dynamic constraints. Method: The motor behavior of healthy participants was analysed during two tasks. First, a manual handling task was studied during which participants grasped and moved a crate while standing. This task was realised in a real environment and in VEs with the absence and the presence of haptic information. The latter simulated the physical constraints of the shelf and the crate to be manipulated using a cable-driven robot (Chapter 3, N=12). Second, avatar avoidance tasks were realised when participants walked on a self-paced treadmill in the absence and then in the presence of a risk of physical contact with avatars. Contact was simulated by a cable-driven robot (Chapter 4, N=10). VEs were viewed through a head mounted display for all tasks. Results: The first study showed that adding haptic feedback to the VE improved spatial parameters of movement realised in a VE during all phases of movement (reaching, ascent and descent phases). The spatial organisation of movement was closer to those observed in a physical environment, and better respected environmental constraints (higher clearances from the shelf and longer trajectories). Moreover, movement control was influenced by task precision required to avoid any contact with the shelf in the presence of haptic feedback only. The second study demonstrated that when avoiding avatars in VR, more cautious behavior was measured in the presence of potential physical contact. Participants tended to start their avoidance strategy earlier and increased minimum clearance along with a larger personal space regardless of the avatar’s approach angle. Conclusion: Adding haptic feedback in VEs impacts the proactive motor strategies of healthy participants during a manual handling task as well as a locomotor task involving the avoidance of avatars. These results suggest that adding haptic feedback enhances one’s consideration of virtual entities during movement planning. Haptic information imposes more realistic restrictions on the actions afforded by EVs, and likely modifies the perceived consequences of potential contact with virtual entities. It will be important to continue to study the impact of haptic feedback within VEs to provide even more ecological environments to people with motor deficits in order to improve assessment and the consideration of implicit risks posed by the environment

Universités Numériques en région Nord Pas-de-Calais

Communication faite à l\u27occasion de la journée d\u27étude "La bibliothèque à l\u27heure du Web 2.0 : amélioration significative du service aux usagers ?", organisée par l\u27Université d\u27Artois et le groupe de recherche "Document numérique & Usages" (Université Paris 8), Arras, 22 mai 2008

Livre blanc sur les données ouvertes

Ce livre blanc propose de faire un état des lieux sur le mouvement open data et les données ouvertes. Il présente notamment les aspects clés du sujet (juridiques, techniques, économiques), afin que tous ceux qui veulent se lancer dans l’aventure de l’open data puissent mieux en évaluer la nature et les multiples facettes

A combination of LongSAGE with Solexa sequencing is well suited to explore the depth and the complexity of transcriptome

<p>Abstract</p> <p>Background</p> <p>"Open" transcriptome analysis methods allow to study gene expression without <it>a priori </it>knowledge of the transcript sequences. As of now, SAGE (Serial Analysis of Gene Expression), LongSAGE and MPSS (Massively Parallel Signature Sequencing) are the mostly used methods for "open" transcriptome analysis. Both LongSAGE and MPSS rely on the isolation of 21 pb tag sequences from each transcript. In contrast to LongSAGE, the high throughput sequencing method used in MPSS enables the rapid sequencing of very large libraries containing several millions of tags, allowing deep transcriptome analysis. However, a bias in the complexity of the transcriptome representation obtained by MPSS was recently uncovered.</p> <p>Results</p> <p>In order to make a deep analysis of mouse hypothalamus transcriptome avoiding the limitation introduced by MPSS, we combined LongSAGE with the Solexa sequencing technology and obtained a library of more than 11 millions of tags. We then compared it to a LongSAGE library of mouse hypothalamus sequenced with the Sanger method.</p> <p>Conclusion</p> <p>We found that Solexa sequencing technology combined with LongSAGE is perfectly suited for deep transcriptome analysis. In contrast to MPSS, it gives a complex representation of transcriptome as reliable as a LongSAGE library sequenced by the Sanger method.</p

Les Sciences Humaines et Sociales dans le PRES LSE

Ce rapport donne un aperçu général sur l'activité de recherche en sciences humaines et sociales dans le Pôle de Recherche et d'Enseignement Supérieur de Lyon - Saint-Etienne. Cette description porte à la fois sur les ressources humaines engagées, sur la production scientifique qui en résulte et sur les intéractions entre les acteurs de la recherches eux-mêmes

In Subfertile Couple, Abdominal Fat Loss in Men Is Associated with Improvement of Sperm Quality and Pregnancy: A Case-Series

International audienceBackground: The impact of overweight among men of reproductive-age may affect fertility. Abdominal fat, more than body mass index, is an indicator of higher metabolic risk, which seems to be involved in decreasing sperm quality. This study aims to assess the relationship between abdominal fat and sperm DNA fragmentation and the effect of abdominal fat loss, among 6 men in subfertile couples. Methods: Sperm DNA fragmentation, abdominal fat and metabolic and hormonal profiles were measured in the 6 men before and after dietary advices. Seminal oxidative stress and antioxidant markers were determined. Results: After several months of a lifestyle program, all 6 men lost abdominal fat (patient 1: loss of 3 points of abdominal fat, patient 2: loss of 3 points, patient 3: loss of 2 points, patient 4: loss of 1 point, patient 5: loss of 4 points and patient 6: loss of 13 points). At the same time, their rate of sperm DNA fragmentation decreased: 9.5% vs 31%, 24% vs 43%, 18% vs 47%, 26.3% vs 66%, 25.4% vs 35% and 1.7% vs 25%. Also, an improvement in both metabolic (significant decrease in triglycerides and total cholesterol; p = 0.0139) and hormonal (significant increase in testosterone/oestradiol ratio; p = 0.0139) blood profiles was observed after following the lifestyle program. In seminal plasma, the amount of SOD2 has significantly increased (p = 0.0139) while in parallel carbonylated proteins have decreased. Furthermore, all spouses got pregnant. All pregnancies were brought to term. Conclusion: This study shows specifically that sperm DNA fragmentation among men in subfertile couples could be affected by abdominal fat, but improvement of lifestyle factor may correct this alteration. The effect of specific abdominal fat loss on sperm quality needs further investigation. The reduction of oxidative stress may be a contributing factor

Biotransformations of bisphenol A in a mammalian model: answers and new questions raised by low-dose metabolic fate studies in pregnant CD1 mice.

We investigated the metabolic fate of a low dose (25 micro g/kg) of bisphenol A [2,2-bis(4-hydroxy-phenyl)propane] (BPA) injected subcutaneously in CD1 pregnant mice using a tritium-labeled molecule. Analytic methods were developed to allow a radio-chromatographic profiling of BPA residues in excreta and tissues, as well as in mothers' reproductive tracts and fetuses, that contained more than 4% of the administered radioactivity. BPA was extensively metabolized by CD1 mice. Identified metabolite structures included the glucuronic acid conjugate of BPA, several double conjugates, and conjugated methoxylated compounds, demonstrating the formation of potentially reactive intermediates. Fetal radioactivity was associated with unchanged BPA, BPA glucuronide, and a disaccharide conjugate. The latter structure, as well as that of a dehydrated glucuronide conjugate of BPA (a major metabolite isolated from the digestive tract), showed that BPA metabolic routes were far more complex than previously thought. The estrogenicity of the metabolites that were identified but not tested for hormonal activity cannot be ruled out; however, in general, conjugated BPA metabolites have significantly lower potency than that of the parent compound. Thus, these data suggest the parental compound is responsible for the estrogenic effects observed in fetuses exposed to BPA during gestation in this mammalian model

Bovine tuberculosis in wildlife in France. Risk for cattle

Tuberculosis (TB) due to M. bovis has been described in wildlife species of many countries, albeit, depending on the situation, being considered as a sentinel or a reservoir of the infection for livestock and/or human. In France, TB has been discovered for the first time, in 2001, in wild ungulates of the Brotonne Forest (Normandy). Since then, passive and active surveillance for the disease has been implemented in several regions, and later expanded to the whole country through the Sylvatub surveillance program since 2011. Firstly described in wild boar and red deer, it has also been detected in 2009 in badgers of Côte d’Or, then in 2010 in Dordogne and Charente. The presence of high density populations of wild animals and some practices as artificial feeding, which lead to non-natural high concentrations of animals, are major factors of emergence and persistence of TB in wildlife. In several French regions, M. bovis would be circulating in a multi-host system including bovines (and pigs, in Corsica only), several wildlife species and their environment which thus complicates the epidemiology and sanitary management of the diseaseLa tuberculose à Mycobacterium bovis (TB) est décrite dans la faune sauvage de plusieurs pays, celle-ci pouvant être, selon les cas, sentinelle ou réservoir de l’infection pour les bovins et/ou l’homme. En France, la TB a été découverte en 2001 chez des ongulés sauvages en forêt de Brotonne (Normandie). Depuis cette date, une surveillance événementielle et programmée de la maladie a été mise en place dans plusieurs départements, puis étendue de façon harmonisée à tout le territoire français métropolitain grâce à la mise en place du dispositif Sylvatub depuis 2011. D’abord décrite chez les cerfs et les sangliers, la TB a été détectée chez le blaireau en 2009 en Côte d’Or, puis en 2010 en Dordogne et en Charente. La présence de fortes densités d’animaux sauvages et entre autres la pratique de l’agrainage, qui provoque des concentrations artificielles d’animaux, sont des facteurs majeurs d’émergence et de persistance de la TB dans les populations sauvages. Dans plusieurs régions de France, M. bovis semble circuler dans un système multi-hôtes comprenant les bovins (et les porcs en Corse uniquement), plusieurs espèces sauvages et leur environnement ce qui complique l’épidémiologie et la gestion sanitaire de la maladi

GWAS in the SIGNAL/PHARE clinical cohort restricts the association between the FGFR2 locus and estrogen receptor status to HER2-negative breast cancer patients

International audienceGenetic polymorphisms are associated with breast cancer risk. Clinical and epidemiological observations suggest that clinical characteristics of breast cancer, such as estrogen receptor or HER2 status, are also influenced by hereditary factors. To identify genetic variants associated with pathological characteristics of breast cancer patients, a Genome Wide Association Study was performed in a cohort of 9365 women from the French nationwide SIGNAL/PHARE studies (NCT00381901/RECF1098). Strong association between the FGFR2 locus and ER status of breast cancer patients was observed (ER-positive n=6211, ER-negative n=2516; rs3135718 OR=1.34 p=5.46x10-12). This association was limited to patients with HER2-negative tumors (ER-positive n=4267, ER-negative n=1185; rs3135724 OR=1.85 p=1.16x10-11). The FGFR2 locus is known to be associated with breast cancer risk. This study provides sound evidence for an association between variants in the FGFR2 locus and ER status among breast cancer patients, particularly among patients with HER2-negative disease. This refinement of the association between FGFR2 variants and ER-status to HER2-negative disease provides novel insight to potential biological and clinical influence of genetic polymorphisms on breast tumors

Infection of Wildlife by Mycobacterium bovis in France Assessment Through a National Surveillance System, Sylvatub

Mycobacterium bovis infection was first described in free-ranging wildlife in France in 2001, with subsequent detection in hunter-harvested ungulates and badgers in areas where outbreaks of bovine tuberculosis (TB) were also detected in cattle. Increasing concerns regarding TB in wildlife led the French General Directorate for Food (DGAL) and the main institutions involved in animal health and wildlife management, to establish a national surveillance system for TB in free-ranging wildlife. This surveillance system is known as “Sylvatub.” The system coordinates the activities of various national and local partners. The main goal of Sylvatub is to detect and monitor M. bovis infection in wildlife through a combination of passive and active surveillance protocols adapted to the estimated risk level in each area of the country. Event-base surveillance relies on M. bovis identification (molecular detection) (i) in gross lesions detected in hunter-harvested ungulates, (ii) in ungulates that are found dead or dying, and (iii) in road-killed badgers. Additional targeted surveillance in badgers, wild boars and red deer is implemented on samples from trapped or hunted animals in at-risk areas. With the exception of one unexplained case in a wild boar, M. bovis infection in free-living wildlife has always been detected in the vicinity of cattle TB outbreaks with the same genotype of the infectious M. bovis strains. Since 2012, M. bovis was actively monitored in these infected areas and detected mainly in badgers and wild boars with apparent infection rates of 4.57–5.14% and 2.37–3.04%, respectively depending of the diagnostic test used (culture or PCR), the period and according to areas. Sporadic infection has also been detected in red deer and roe deer. This surveillance has demonstrated that M. bovis infection, in different areas of France, involves a multi-host system including cattle and wildlife. However, infection rates are lower than those observed in badgers in the United Kingdom or in wild boars in Spain