298 research outputs found
Structural Loop Between the Cerebellum and the Superior Temporal Sulcus: Evidence from Diffusion Tensor Imaging
The cerebellum is believed to play an essential role in a variety of motor and cognitive functions through reciprocal interaction with the cerebral cortex. Recent findings suggest that cerebellar involvement in the network specialized for visual body motion processing may be mediated through interaction with the right superior temporal sulcus (STS). Yet, the underlying pattern of structural connectivity between the STS and the cerebellum remains unidentified. In the present work, diffusion tensor imaging analysis on seeds derived from functional magnetic resonance imaging during a task on point-light biological motion perception uncovers a structural pathway between the right posterior STS and the left cerebellar lobule Crus I. The findings suggest existence of a structural loop underpinning bidirectional communication between the STS and cerebellum. This connection might also be of potential value for other visual social abilitie
Stressor- and Corticotropin releasing Factor-induced Reinstatement and Active Stress-related Behavioral Responses are Augmented Following Long-access Cocaine Self-administration by Rats
Rationale Stressful events during periods of drug abstinence likely contribute to relapse in cocaine-dependent individuals. Excessive cocaine use may increase susceptibility to stressor-induced relapse through alterations in brain corticotropin-releasing factor (CRF) responsiveness.
Objectives This study examined stressor- and CRF-induced cocaine seeking and other stress-related behaviors in rats with different histories of cocaine self-administration (SA).
Materials and methods Rats self-administered cocaine under short-access (ShA; 2 h daily) or long-access (LgA; 6 h daily) conditions for 14 days or were provided access to saline and were tested for reinstatement by a stressor (electric footshock), cocaine or an icv injection of CRF and for behavioral responsiveness on the elevated plus maze, in a novel environment and in the lightâdark box after a 14- to 17-day extinction/withdrawal period.
Results LgA rats showed escalating patterns of cocaine SA and were more susceptible to reinstatement by cocaine, EFS, or icv CRF than ShA rats. Overall, cocaine SA increased activity in the center field of a novel environment, on the open arms of the elevated plus maze, and in the light compartment of a lightâdark box. In most cases, the effects of cocaine SA were dependent on the pattern/amount of cocaine intake with statistically significant differences from saline self-administering controls only observed in LgA rats.
Conclusions When examined after several weeks of extinction/ withdrawal, cocaine SA promotes a more active pattern of behavior during times of stress that is associated with a heightened susceptibility to stressor-induced cocaine-seeking behavior and may be the consequence of augmented CRF regulation of addiction-related neurocircuitry
Distinct Relationship Between Cognitive Flexibility and White Matter Integrity in Individuals at Risk of Parkinsonâs Disease
Background and Objective: Executive dysfunction is the most common cognitive impairment in Parkinsonâs disease (PD), occurring even in its early stages. In our study, we applied diffusion tensor imaging (DTI) to investigate white matter integrity and its association with a specific executive function such as cognitive flexibility in individuals with risk factors for PD.
Methods: We examined 50 individuals with risk factors for developing PD and 24 healthy controls from the TREND (TĂŒbinger Evaluation of Risk Factors for Early Detection of Neurodegeneration) study including neuropsychological evaluation and DTI. Cognitive flexibility was assessed using the trail making test (TMT). Tract based spatial statistics (TBSS) were employed to assess white matter abnormalities and their correlation with cognitive flexibility.
Results: TMT performance correlated with mean and axial diffusivity in several white matter regions, predominantly in the frontoparietal white matter. These effects were stronger in PD risk persons (PD-RP) than in controls as evidenced by a significant group interaction. White matter integrity and TMT performance did not significantly differ across groups.
Conclusion: Based on our results, PD-RP do no exhibit white matter changes or impaired cognitive flexibility. However, specific executive functions in PD-RP are more related to white matter alterations than in healthy older adults
Gait decline while dual-tasking is an early sign of white matter deterioration in middle-aged and older adults
Loss of white matter integrity (WMI) is associated with gait deficits in middle-aged and older adults. However, these deficits are often only apparent under cognitively demanding situations, such as walking and simultaneously performing a secondary cognitive task. Moreover, evidence suggests that declining executive functions (EF) are linked to gait decline, and their co-occurrence may point to a common underlying pathology, i.e., degeneration of shared brain regions. In this study, we applied diffusion tensor imaging (DTI) and a standardized gait assessment under single- and dual-tasking (DT) conditions (walking and subtracting) in 74 middle-aged and older adults without any significant gait or cognitive impairments to detect subtle WM alterations associated with gait decline under DT conditions. Additionally, the Trail Making Test (TMT) was used to assess EF, classify participants into three groups based on their performance, and examine a possible interaction between gait, EF, and WMI. Gait speed and subtracting speed while dual-tasking correlated significantly with the fractional anisotropy (FA) in the bilateral anterior corona radiata (highest r = 0.51/p < 0.0125 FWE-corrected). Dual-task costs (DTC) of gait speed correlated significantly with FA in widespread pathways, including the corpus callosum, bilateral anterior and superior corona radiata, as well as the left superior longitudinal fasciculus (highest r = â0.47/p < 0.0125 FWE-corrected). EF performance was associated with FA in the left anterior corona radiata (p < 0.05); however, EF did not significantly mediate the effects of WMI on DTC of gait speed. There were no significant correlations between TMT and DTC of gait and subtracting speed, respectively. Our findings indicate that gait decline under DT conditions is associated with widespread WM deterioration even in middle-aged and older adults without any significant gait or cognitive impairments
Insights into the mechanism of cell death induced by saporin delivered into cancer cells by an antibody fusion protein targeting the transferrin receptor 1
We previously developed an antibody-avidin fusion protein (ch128.1Av) that targets the human transferrin receptor 1 (TfR1) and exhibits direct cytotoxicity against malignant B cells in an iron-dependent manner. ch128.1Av is also a delivery system and its conjugation with biotinylated saporin (b-SO6), a plant ribosome-inactivating toxin, results in a dramatic iron-independent cytotoxicity, both in malignant cells that are sensitive or resistant to ch128.1Av alone, in which the toxin effectively inhibits protein synthesis and triggers caspase activation. We have now found that the ch128.1Av/b-SO6 complex induces a transcriptional response consistent with oxidative stress and DNA damage, a response that is not observed with ch128.1Av alone. Furthermore, we show that the antioxidant N-acetylcysteine partially blocks saporin-induced apoptosis suggesting that oxidative stress contributes to DNA damage and ultimately saporin-induced cell death. Interestingly, the toxin was detected in nuclear extracts by immunoblotting, suggesting the possibility that saporin might induce direct DNA damage. However, confocal microscopy did not show a clear and consistent pattern of intranuclear localization. Finally, using the long-term culture-initiating cell assay we found that ch128.1Av/b-SO6 is not toxic to normal human hematopoietic stem cells suggesting that this critical cell population would be preserved in therapeutic interventions using this immunotoxin.Fil: Daniels Wells, Tracy R.. University of California; Estados Unidos de AmĂ©rica;Fil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de BiologĂa y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y BioquĂmica; Argentina. University of California; Estados Unidos de AmĂ©rica;Fil: RodrĂguez, JosĂ© A.. University of California; Estados Unidos de AmĂ©rica;Fil: Leoh, Lai Sum. University of California; Estados Unidos de AmĂ©rica;Fil: Erb, Michael A.. University of California; Estados Unidos de AmĂ©rica;Fil: Diamante, Graciel. University of California; Estados Unidos de AmĂ©rica;Fil: Casero, David. University Of California; Estados Unidos de AmĂ©rica;Fil: Pellegrini, Mateo. University of California; Estados Unidos de AmĂ©rica;Fil: Martinez Maza, Otoniel. University of California; Estados Unidos de AmĂ©rica;Fil: Penichet, Manuel L.. University of California; Estados Unidos de AmĂ©rica
Hubble Space Telescope WFC3 Early Release Science: Emission-Line Galaxies from Infrared Grism Observations
We present grism spectra of emission-line galaxies (ELGs) from 0.6-1.6
microns from the Wide Field Camera 3 on the Hubble Space Telescope. These new
infrared grism data augment previous optical Advanced Camera for Surveys G800L
0.6-0.95 micron grism data in GOODS-South from the PEARS program, extending the
wavelength covereage well past the G800L red cutoff. The ERS grism field was
observed at a depth of 2 orbits per grism, yielding spectra of hundreds of
faint objects, a subset of which are presented here. ELGs are studied via the
Ha, [OIII], and [OII] emission lines detected in the redshift ranges 0.2<z<1.4,
1.2<z<2.2 and 2.0<z<3.3 respectively in the G102 (0.8-1.1 microns; R~210) and
G141 (1.1-1.6 microns; R~130) grisms. The higher spectral resolution afforded
by the WFC3 grisms also reveals emission lines not detectable with the G800L
grism (e.g., [SII] and [SIII] lines). From these relatively shallow
observations, line luminosities, star-formation rates, and grism spectroscopic
redshifts are determined for a total of 48 ELGs to m(AB)~25 mag. Seventeen
GOODS-South galaxies that previously only had photometric redshifts now have
new grism-spectroscopic redshifts, in some cases with large corrections to the
photometric redshifts (Delta(z)~0.3-0.5). Additionally, one galaxy had no
previously-measured redshift but now has a secure grism-spectroscopic redshift,
for a total of 18 new GOODS-South spectroscopic redshifts. The faintest source
in our sample has a magnitude m(AB)=26.9 mag. The ERS grism data also reflect
the expected trend of lower specific star formation rates for the highest mass
galaxies in the sample as a function of redshift, consistent with downsizing
and discovered previously from large surveys. These results demonstrate the
remarkable efficiency and capability of the WFC3 NIR grisms for measuring
galaxy properties to faint magnitudes and redshifts to z>2.Comment: Accepted for publication in AJ. Updated to include referee comments.
Updated sample using improved reduction contains 23 new galaxies (Table 1;
Figures 2 & 3
Local Lyman Break Galaxy Analogs: The Impact of Massive Star-forming Clumps on the Interstellar Medium and the Global Structure of Young, Forming Galaxies
We present HST UV/optical imaging, Spitzer mid-IR photometry, and optical
spectroscopy of a sample of 30 low-redshift (z=0.1-0.3) galaxies chosen from
SDSS/GALEX to be accurate local analogs of the high-z Lyman Break Galaxies. The
Lyman Break Analogs (LBAs) are similar in mass, metallicity, dust, SFR, size
and gas velocity dispersion, thus enabling a detailed investigation of
processes that are important at high-z. The optical emission line properties of
LBAs are also similar to those of LBGs, indicating comparable conditions in
their ISM. In the UV, LBAs are characterized by complexes of massive
star-forming "clumps", while in the optical they most often show evidence for
(post-)mergers/interactions. In 6 cases, we find an extremely massive (>10^9
Msun) compact (R~100 pc) dominant central object (DCO). The DCOs are
preferentially found in LBAs with the highest mid-IR luminosities and
correspondingly high SFRs (15-100 Msun/yr). We show that the massive SF clumps
(including the DCOs) have masses much larger than the nuclear super star
clusters seen in normal late type galaxies. However, the DCOs have masses,
sizes, and densities similar to the excess-light/central-cusps seen in typical
elliptical galaxies with masses similar to the LBA galaxies. We suggest that
the DCOs form in present-day examples of the dissipative mergers at high
redshift that are believed to have produced the central-cusps in local
ellipticals. More generally, the properties of the LBAs are consistent with the
idea that instabilities in a gas-rich disk lead to very massive star-forming
clumps that eventually coalesce to form a spheroid. We speculate that the DCOs
are too young at present to be growing a supermassive black hole because they
are still in a supernova-dominated outflow phase.Comment: The Astrophysical Journal, In Press (22 pages, 16 figures). For the
full version with high-resolution colour figures, see:
http://www.mpa-garching.mpg.de/~overzier/Overzier_LBApaper09.pd
Additions to the Last Millennium Reanalysis Multi-Proxy Database
Progress in paleoclimatology increasingly occurs via data syntheses. We describe additions to a collection prepared for use in paleoclimate state estimation, specifically the Last Millennium Reanalysis (LMR). The 2290 additional series include 2152 tree ring chronologies and 138 other series. They supplement the collection used previously and together form a database titled LMRdb 1.0.0. The additional data draws from lake core, ice core, coral, speleothem, and tree ring archives, using published data primarily from the NOAA Paleoclimatology archive and a set of tree ring width chronologies standardized from raw International Tree Ring Data Bank ring width series. In contrast to many previous paleo compilations, the data were not selected (screened) on the basis of their environmental correlation, multi-century length, or other attributes. The inclusion of proxies sensitive to moisture and other environmental variables expands their use in data assimilation. A preliminary calibration using linear regression with mean annual temperature reveals characteristics of the proxy series and their relationship to temperature, as well as the noise and error characteristics of the records. The additional records are structured as individual files in the NOAA Paleoclimatology format and archived at NOAA Paleoclimatology (Anderson et al. 2018) and will continue to be improved and expanded as part of the LMR Project. The additions represent a four-fold increase in the number of records available for assimilation, provide expanded geographic coverage, and add additional proxy variables. Applications include data assimilation, proxy system model development, and paleoclimate reconstruction using climate field reconstruction and other methods
The Epoch of Disk Settling: z~1 to Now
We present evidence from a sample of 544 galaxies from the DEEP2 Survey for
evolution of the internal kinematics of blue galaxies with stellar masses
ranging 8.0 < log M* (M_Sun) < 10.7 over 0.2<z<1.2. DEEP2 provides galaxy
spectra and Hubble imaging from which we measure emission-line kinematics and
galaxy inclinations, respectively. Our large sample allows us to overcome
scatter intrinsic to galaxy properties in order to examine trends in
kinematics. We find that at a fixed stellar mass galaxies systematically
decrease in disordered motions and increase in rotation velocity and potential
well depth with time. Massive galaxies are the most well-ordered at all times
examined, with higher rotation velocities and less disordered motions than less
massive galaxies. We quantify disordered motions with an integrated gas
velocity dispersion corrected for beam smearing (sigma_g). It is unlike the
typical pressure-supported velocity dispersion measured for early type galaxies
and galaxy bulges. Because both seeing and the width of our spectral slits
comprise a significant fraction of the galaxy sizes, sigma_g integrates over
velocity gradients on large scales which can correspond to non-ordered gas
kinematics. We compile measurements of galaxy kinematics from the literature
over 1.2<z<3.8 and do not find any trends with redshift, likely for the most
part because these datasets are biased toward the most highly star-forming
systems. In summary, over the last ~8 billion years since z=1.2, blue galaxies
evolve from disordered to ordered systems as they settle to become the
rotation-dominated disk galaxies observed in the Universe today, with the most
massive galaxies being the most evolved at any time.Comment: submitted to ApJ and responded to referee repor
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