El Hombre Ave, el Robot y una búsqueda por el cuidado en las dinámicas de poder entre directora y performer

La presente investigación busca identificar y analizar cuáles fueron los mecanismos implementados para buscar asegurar el cuidado en las dinámicas de poder entre directora performer en la obra peruana "El Hombre Ave y el Robot" (2021). La obra escénica y audiovisual analizada partió de la escenificación del vínculo fraternal entre Alfredo, una persona neurodiverso, y yo, su hermana mayor. Esta escenificación tuvo dos grandes puntos de partida: Lo documental y la creación conjunta de una narrativa fantástica. Se encontró que los cuestionamientos éticos en la relación directora-performer se complejizan por sus varias vertientes: El hecho que también asumí el rol de editora y documentalista, Alfredo siendo neurodiverso y yo neurotípica y finalmente, que se trata de una relación fraternal. Esto llevó a potenciales cuestionamientos morales cómo el peligro de caer en estereotipos, el deber moral del documentalista para representar una realidad “objetiva”, y el debate que trae consigo el buscar representar las facetas afectivas y vulnerables de una relación. Posteriormente, se analizaron tres mecanismos implementados durante el proceso para identificar de qué formas cuidan estas dinámicas de poder: La entrada multidimensional a la representación, el juego como herramienta predilecta de escenificación, y la creación colaborativa. Se logró concluir que si bien las jerarquías en los procesos de creación son ineludibles, la implementación de estos mecanismos, en conjunto con el objetivo del proceso de crear un producto colaborativo y conjunto, logró generar un espacio de mayor libertad y en donde ambos teníamos agencia creativa. El mostrar este proceso ante el espectador también invitó a la reflexión y cuestionamiento de estereotipos y discursos hegemónicos sobre lo neurodiverso, lo neurotípico, y el poder cómo meramente vertical.This research-based paper seeks to identify and analyze what mechanisms were implemented to ensure an ethical care in the dynamics of power between director and performer in the Peruvian play "El Hombre Ave y el Robot" (2021). This theatrical and audiovisual play took as a starting point the representation of the fraternal bond between a neurodiverse subject, Alfredo, and me, his older sister. This process had two axes: the documentary and the joint creation of a fantastic narrative. It was found that ethical questions in the director-performer relationship are made more complex by the fact that it contains various aspects: The fact that I also assumed the role of editor and documentarian, Alfredo being neurodiverse and I neurotypical, and finally, that it was a fraternal relationship. This led to potential moral questioning such as the danger of representation stereotypes, the documentary filmmaker's moral duty to represent an “objective” reality, and the debate that comes with seeking to represent the affective and vulnerable facets of a relationship. Subsequently, three mechanisms implemented during the process were analyzed to identify in what ways they looked out for these ethical dilemmas: The multidimensional entrance to the representation, play as the most utilized tool of representation, and collaborative creation. It was possible to conclude that although the hierarchies in creation processes are inescapable, the implementation of these mechanisms, together with the process´ objective to create a collaborative and jointly created product, managed to generate a space of greater freedom and where we both had creative agency. Showing this process to the spectator also invited reflection and questioning of stereotypes and hegemonic discourses regarding neurodiversity, the neurotypical, and the application of power as solely a vertical practice

Alfredo e Inés: El Hombre Ave y el Robot O una exploración escénica de identidades, neurodiversidad, mundos fantásticos y dos hermanos

Esta investigación desde la práctica artística se propuso encontrar mecanismos para construir escénicamente el vínculo fraternal con un sujeto neurodiverso en una creación colaborativa que se nutrió del género documental y fantástico e interactuó con elementos audiovisuales. Dicha investigación, planteó una exploración escénica del vínculo a partir de dos entradas: lo documental y la creación conjunta de una narrativa fantástica. Metodológicamente, las dos entradas se materializaron a través de entrevistas a miembros de la familia nuclear, la realización de un registro audiovisual permanente y el desarrollo de un laboratorio de creación que buscó generar una narrativa fantástica, para luego encontrar mecanismos para su escenificación. La sistematización del material generado decantó en una obra escénica/audiovisual titulada El Hombre Ave y el Robot. En ella, el vínculo fraternal se construyó escénicamente a través de la creación de identidades performativas y flexibles que transitaban entre dos hermanos reales (Inés y Alfredo), su cotidianidad, las aventuras de dos personajes (El Hombre Ave y el Robot), y un universo fantástico creado conjuntamente. A través de la vía poética de lo fantástico y el cuestionamiento de una realidad objetiva en lo documental, se fueron revelando escénicamente las dimensiones afectivas de ambos, sus percepciones subjetivas del mundo y la relación en sí. Se encontró que lo escénico no sólo permitió una construcción representativa de la relación, sino una vía de comunicación, de expresión performática libre y de continuo desarrollo del vínculo fraternal. Finalmente, la experiencia como obra escénica se convirtió en una invitación al público para reflexionar respecto a su propia forma de relacionarse con otro y volver a pensar conceptos como lo normal, y lo diferente, cuestionándolos como conceptos absolutos y definitorios de una identidad individual

Biological observations of the tope shark, Galeorhinus galeus , in the northern Patagonian gulfs of Argentina

During 1994–96, an experimental longline fishery for tope shark (Galeorhinus galeus) was carried out in the Nuevo Gulf of northern Patagonia and, in the summers of 2000–2001 and 2002, three boats conducted an exploratory commercial fishery for this species, accompanied by a scientific monitoring program. This paper summarizes the results of these fishing trials, and provides information on the biology of tope shark. Catches were highest from February to April, when tope shark represented 36% of the total fish caught, and elephant fish (Callorhynchus callorhynchus) and argentine hake (Merluccius hubbsi) accounted for 33% and 23%, respectively. Tope shark arriving in northern Patagonian waters during the summer are primarily mature males, immature and maturing females in their first and second non-gravid year. No gravid females were caught. These fish are part of the South-western Atlantic stock, which shows signs of over-exploitation, so we suggest that any longline fishery in Patagonia should remain on a small scale. We also recommend that an effective management plan is needed for the whole tope stock, establishing agreements on effort control and co-ordinated research between Brazil, Uruguay and Argentina.Fil: Elias, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; ArgentinaFil: Rodriguez, A. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Hasan, E.. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Reyna, M. V.. Universidad Nacional de la Patagonia "San Juan Bosco"; ArgentinaFil: Amoroso, Ricardo Oscar. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentin

Titin domains with reduced core hydrophobicity cause dilated cardiomyopathy.

The underlying genetic defect in most cases of dilated cardiomyopathy (DCM), a common inherited heart disease, remains unknown. Intriguingly, many patients carry single missense variants of uncertain pathogenicity targeting the giant protein titin, a fundamental sarcomere component. To explore the deleterious potential of these variants, we first solved the wild-type and mutant crystal structures of I21, the titin domain targeted by pathogenic variant p.C3575S. Although both structures are remarkably similar, the reduced hydrophobicity of deeply buried position 3575 strongly destabilizes the mutant domain, a scenario supported by molecular dynamics simulations and by biochemical assays that show no disulfide involving C3575. Prompted by these observations, we have found that thousands of similar hydrophobicity-reducing variants associate specifically with DCM. Hence, our results imply that titin domain destabilization causes DCM, a conceptual framework that not only informs pathogenicity assessment of gene variants but also points to therapeutic strategies counterbalancing protein destabilization.S

Proposal of Equations for Predicting Post-Farrowing Sow Weight

Background: Body condition score is used widely in swine production to ensure adequate nutritional levels in sows during gestation and lactation. However, body condition score is not a gold standard for the estimation of nutritional requirements in sows. Post-farrowing sow body weight assessment might serve as a useful approach for the better adjustment of the nutritional requirements during lactation; however, this approach is time-consuming, requires labor, and might result in detrimental effects on the sow behavior and welfare. The objective of the present study, therefore, was to formulate prediction equations for the estimation of post-farrowing sow weight.Materials, Methods & Results: Seven equations were formulated for predicting the post-farrowing sow body weight, by using the data from three databases, which comprised a total 522 sows (434 gilts and 88 multiparous). The sows were weighed on Day 112 of gestation and after farrowing within 12 h. The piglets birth weight was recorded within 24 h after farrowing. The equations were formulated considering all the parity orders. While formulating the equations, the following five variables were used: pre-farrowing body weight, piglets born, litter weight, the interval between pre-farrowing weighing and farrowing (in days), and the total feed intake between pre-farrowing and post-farrowing weighing. The seven models were compared using the sets of possible predictors through regression with the best subsets procedure (Minitab for Windows, v. 18). Equations (EQ) 1, 2, and 4 were validated with a database comprising 732 sows (parity orders: 1–5). The females were weighed on Day 107 of gestation and within 24 h after farrowing. The predicted weights estimated by EQ 2 and 4 (215.4 ± 34.3 kg and 216.7 ± 34.4 kg, respectively) did not significantly differ from the observed weight (216.8 ± 34.6 kg) [P > 0.05].Discussion: Pre-farrowing sow body weight was identified as the main input variable required for the estimation of the post-farrowing sow body weight. Thus, even EQ 1, which contained only this variable, exhibited a high coefficient of determination (R2 = 0.8707). However, the R2 value kept increasing as more input variables were included in the equation. Equation 2, 4, and 6 included the litter weight variable, and the addition of this variable increased the numerical value of R2 from 0.8707 in EQ 1 to 0.8975 in EQ 2. The EQ 3, 5, and 7 considered the piglets born variable as well, which increased the R2 value from 0.8707 in EQ 1 to 0.9119 in EQ 3. The coefficient of determination did not vary much among the equations; therefore, the selection of the prediction equations depended on data availability, feed management, facility, and the reliability of data collection in each farm. Although EQ 1 demonstrated a greater correlation between the predicted and the observed post-farrowing weight compared to the other equations, the values of error in central tendency and the errors due to disturbances were numerically higher for EQ 1 in comparison to the other two equations (EQ 2 and 4). Therefore, it is suggested that EQ 1 should be used as the last choice for the estimation of post-farrowing sow weight as it presented low trueness and precision, and also because the predicted weight estimated by EQ 1 was statistically lower than the observed weight (211.67 ± 33.33 kg vs. 216.84 ± 34.62 kg; P = 0.012). EQ 4 emonstrated higher trueness and precision; however, it did not differ significantly from EQ 2 and 1. Further analyses are required in order to validate EQ 3, 5, 6, and 7. Among the equations that were predicted as well as validated, the simplest and the easiest equation with satisfactory results for trueness and precision was EQ 2, which is as follows:Post-farrowing sow weight (kg) = 13.03 + (0.93 × pre-farrowing body weight, kg) + (–1.23 × piglets born, n

T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease

Chagas disease (American trypanosomiasis) is caused by the protozoan parasite Trypanosoma cruzi and represents a major public health problem in Latin America. Furthermore, growing human population movements extend the disease distribution to regions outside the South American continent. Accurate diagnosis is crucial in patient care and in preventing transmission through blood transfusion, organ transplantation, or vertical transmission from mother to child. Routine diagnosis of Trypanosoma cruzi infection generally is based on detection of the host's antibodies against the parasite. However, antibody detection tests are liable to specificity problems and are of limited use in assessing treatment outcome and congenital infections. The introduction of the polymerase chain reaction (PCR) to amplify specific DNA sequences opened promising diagnostic perspectives. Despite its reported high sensitivity and specificity, broad use of the PCR technique in diagnosis of Chagas disease is hampered by its complexity and the lack of any standardization. We here present the development and evaluation of the T. cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR amplified T. cruzi DNA. The new tool is an important step towards simplified and standardized molecular diagnosis of Chagas disease

Building Bridges for Ocean Sustainability: The Evolution and Impact of the Early Career Ocean Professional (ECOP) Programme

7 pages, 2 figuresSince its endorsement as a global network Programme in June 2021, the Early Career Ocean Professional (ECOP) Programme has been instrumental in supporting ocean professionals worldwide through funding, training, networking opportunities, and career development initiatives. Rooted in the vision of elevating diverse perspectives, the ECOP Programme fosters knowledge exchange between experienced professionals and ECOPs to promote ocean sustainability. As the UN Decade of Ocean Science for Sustainable Development (2021-2030) enters its fourth year of implementation, the ECOP Programme has grown and diversified to a formidable extent, both geographically and thematically. With over 5,200 ECOPs from 151 countries and overseas territories, the global ECOP community is currently dispersed across five regional nodes and more than 40 national hubs. This commentary brings some of the most active ECOP nodes into the limelight, amplifying their collective efforts to better understand, engage, and empower the next generation of ocean professionals across different cultural contexts. This is also an opportunity to showcase the successes, milestones, and challenges that ECOP nodes have encountered over the past 2 years, including on thematic priorities such as communications and outreach, capacity development, and ocean literacy. Moving forward, the ECOP Programme aims to further expand geographically, enhance funding capacities, deepen capacity development efforts, and promote greater awareness of the UN Ocean Decade ecosystem and its vast portfolio of endorsed Actions. As the next generation of ocean professionals will inherit our interconnected ocean beyond 2030, building trust, transferring knowledge, and taking true ownership of the global ocean agenda are paramountWith the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewe

Risk factors for infections caused by carbapenem-resistant Enterobacterales: an international matched case-control-control study (EURECA)

Cases were patients with complicated urinary tract infection (cUTI), complicated intraabdominal (cIAI), pneumonia or bacteraemia from other sources (BSI-OS) due to CRE; control groups were patients with infection caused by carbapenem-susceptible Enterobacterales (CSE), and by non-infected patients, respectively. Matching criteria included type of infection for CSE group, ward and duration of hospital admission. Conditional logistic regression was used to identify risk factors. Findings Overall, 235 CRE case patients, 235 CSE controls and 705 non-infected controls were included. The CRE infections were cUTI (133, 56.7%), pneumonia (44, 18.7%), cIAI and BSI-OS (29, 12.3% each). Carbapenemase genes were found in 228 isolates: OXA-48/like, 112 (47.6%), KPC, 84 (35.7%), and metallo-beta-lactamases, 44 (18.7%); 13 produced two. The risk factors for CRE infection in both type of controls were (adjusted OR for CSE controls; 95% CI; p value) previous colonisation/infection by CRE (6.94; 2.74-15.53; <0.001), urinary catheter (1.78; 1.03-3.07; 0.038) and exposure to broad spectrum antibiotics, as categorical (2.20; 1.25-3.88; 0.006) and time-dependent (1.04 per day; 1.00-1.07; 0.014); chronic renal failure (2.81; 1.40-5.64; 0.004) and admission from home (0.44; 0.23-0.85; 0.014) were significant only for CSE controls. Subgroup analyses provided similar results. Interpretation The main risk factors for CRE infections in hospitals with high incidence included previous coloni-zation, urinary catheter and exposure to broad spectrum antibiotics