Global and local fMRI signals driven by neurons defined optogenetically by type and wiring

Despite a rapidly-growing scientific and clinical brain imaging literature based on functional magnetic resonance imaging (fMRI) using blood oxygenation level-dependent (BOLD) signals, it remains controversial whether BOLD signals in a particular region can be caused by activation of local excitatory neurons. This difficult question is central to the interpretation and utility of BOLD, with major significance for fMRI studies in basic research and clinical applications. Using a novel integrated technology unifying optogenetic control of inputs with high-field fMRI signal readouts, we show here that specific stimulation of local CaMKIIα-expressing excitatory neurons, either in the neocortex or thalamus, elicits positive BOLD signals at the stimulus location with classical kinetics. We also show that optogenetic fMRI (ofMRI) allows visualization of the causal effects of specific cell types defined not only by genetic identity and cell body location, but also by axonal projection target. Finally, we show that ofMRI within the living and intact mammalian brain reveals BOLD signals in downstream targets distant from the stimulus, indicating that this approach can be used to map the global effects of controlling a local cell population. In this respect, unlike both conventional fMRI studies based on correlations and fMRI with electrical stimulation that will also directly drive afferent and nearby axons, this ofMRI approach provides causal information about the global circuits recruited by defined local neuronal activity patterns. Together these findings provide an empirical foundation for the widely-used fMRI BOLD signal, and the features of ofMRI define a potent tool that may be suitable for functional circuit analysis as well as global phenotyping of dysfunctional circuitry

Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures

Elucidation of the neural substrates underlying complex animal behaviors depends on precise activity control tools, as well as compatible readout methods. Recent developments in optogenetics have addressed this need, opening up new possibilities for systems neuroscience. Interrogation of even deep neural circuits can be conducted by directly probing the necessity and sufficiency of defined circuit elements with millisecond-scale, cell type-specific optical perturbations, coupled with suitable readouts such as electrophysiology, optical circuit dynamics measures and freely moving behavior in mammals. Here we collect in detail our strategies for delivering microbial opsin genes to deep mammalian brain structures in vivo, along with protocols for integrating the resulting optical control with compatible readouts (electrophysiological, optical and behavioral). The procedures described here, from initial virus preparation to systems-level functional readout, can be completed within 4–5 weeks. Together, these methods may help in providing circuit-level insight into the dynamics underlying complex mammalian behaviors in health and disease

Do maize crop models catch the impact of future [CO2] on maize yield and water use?

Do maize crop models catch the impact of future [CO2] on maize yield and water use?. iCROPM2016 International Crop Modelling Symposiu

Functional Imaging: CT and MRI

SYNOPSIS: Numerous imaging techniques permit evaluation of regional pulmonary function. Contrast-enhanced CT methods now allow assessment of vasculature and lung perfusion. Techniques using spirometric controlled MDCT allow for quantification of presence and distribution of parenchymal and airway pathology, Xenon gas can be employed to assess regional ventilation of the lungs and rapid bolus injections of iodinated contrast agent can provide quantitative measure of regional parenchymal perfusion. Advances in magnetic resonance imaging (MRI) of the lung include gadolinium-enhanced perfusion imaging and hyperpolarized helium imaging, which can allow imaging of pulmonary ventilation and .measurement of the size of emphysematous spaces

ENQUETE SUR LE CONSENTEMENT ECLAIRE DANS L'ACTE DE SOINS EN ODONTOLOGIE (ETUDE SUR 114 PATIENTS DEPENDANT DU REGIME DE PROTECTION SOCIALE AGRICOLE DANS QUATRE DEPARTEMENTAS BRETONS EN 2000)

RENNES1-BU Santé (352382103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Sortie de "De l'utilité du genre" de Joan Scott

Note de l'éditeur Qu’est-ce que le genre ? Comment les identités sexuelles et les rapports entre hommes et femmes sont-ils construits, et comment se transforment-ils ? Quel rôle jouent, dans ces processus, la politique et les mobilisations collectives, l’économique et le social, mais aussi le langage et l’inconscient ? Historienne mondialement reconnue, Joan W. Scott a imposé l’idée selon laquelle le genre ne constitue pas seulement un domaine d’investigation : c’est un instrument critique de..

3D Printing of Aramid Nanofiber Composites by Stereolithography

Vat photopolymerization is a versatile 3D printing method that produces parts using polymeric materials with uniform mechanical properties, high quality surface finish and high-resolution features. However, it is challenging to make composite materials with vat photopolymerization mainly due to the imperfect filler dispersion in the photo resin. Herein, we describe a methodology to incorporate aramid nanofibers (ANFs) into a 3D printable photoresin as a dispersion, followed by a solvent exchange process that limits anisotropic shrinkage and cracking of the printed polymer. By incorporating 0.60 wt.% of ANFs, both the tensile strength and toughness increased by 264 % and 219 % respectively, while the Young’s modulus had a 406 % increase compared to the control photoresin

Bacterial Interactions in the Context of Chronic Wound Biofilm: A Review

International audienceChronic wounds, defined by their resistance to care after four weeks, are a major concern, affecting millions of patients every year. They can be divided into three types of lesions: diabetic foot ulcers (DFU), pressure ulcers (PU), and venous/arterial ulcers. Once established, the classical treatment for chronic wounds includes tissue debridement at regular intervals to decrease biofilm mass constituted by microorganisms physiologically colonizing the wound. This particular niche hosts a dynamic bacterial population constituting the bed of interaction between the various microorganisms. The temporal reshuffle of biofilm relies on an organized architecture. Microbial community turnover is mainly associated with debridement (allowing transitioning from one major representant to another), but also with microbial competition and/or collaboration within wounds. This complex network of species and interactions has the potential, through diversity in antagonist and/or synergistic crosstalk, to accelerate, delay, or worsen wound healing. Understanding these interactions between microorganisms encountered in this clinical situation is essential to improve the management of chronic wounds