2,460 research outputs found
Maximizing efficiency of rumen microbial protein production.
Rumen microbes produce cellular protein inefficiently partly because they do not direct all ATP toward growth. They direct some ATP toward maintenance functions, as long-recognized, but they also direct ATP toward reserve carbohydrate synthesis and energy spilling (futile cycles that dissipate heat). Rumen microbes expend ATP by vacillating between (1) accumulation of reserve carbohydrate after feeding (during carbohydrate excess) and (2) mobilization of that carbohydrate thereafter (during carbohydrate limitation). Protozoa account for most accumulation of reserve carbohydrate, and in competition experiments, protozoa accumulated nearly 35-fold more reserve carbohydrate than bacteria. Some pure cultures of bacteria spill energy, but only recently have mixed rumen communities been recognized as capable of the same. When these communities were dosed glucose in vitro, energy spilling could account for nearly 40% of heat production. We suspect that cycling of glycogen (a major reserve carbohydrate) is a major mechanism of spilling; such cycling has already been observed in single-species cultures of protozoa and bacteria. Interconversions of short-chain fatty acids (SCFA) may also expend ATP and depress efficiency of microbial protein production. These interconversions may involve extensive cycling of intermediates, such as cycling of acetate during butyrate production in certain butyrivibrios. We speculate this cycling may expend ATP directly or indirectly. By further quantifying the impact of reserve carbohydrate accumulation, energy spilling, and SCFA interconversions on growth efficiency, we can improve prediction of microbial protein production and guide efforts to improve efficiency of microbial protein production in the rumen
A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria
Peer reviewedPublisher PD
'It's a film' : medium specificity as textual gesture in Red road and The unloved
British cinema has long been intertwined with television. The
buzzwords of the transition to digital media, 'convergence' and
'multi-platform delivery', have particular histories in the British
context which can be grasped only through an understanding of the
cultural, historical and institutional peculiarities of the British film
and television industries. Central to this understanding must be two
comparisons: first, the relative stability of television in the duopoly
period (at its core, the licence-funded BBC) in contrast to the repeated
boom and bust of the many different financial/industrial combinations
which have comprised the film industry; and second, the cultural and
historical connotations of 'film' and 'television'. All readers of this
journal will be familiar – possibly over-familiar – with the notion that
'British cinema is alive and well and living on television'. At the end of
the first decade of the twenty-first century, when 'the end of medium
specificity' is much trumpeted, it might be useful to return to the
historical imbrication of British film and television, to explore both
the possibility that medium specificity may be more nationally specific
than much contemporary theorisation suggests, and to consider some
of the relationships between film and television manifest at a textual
level in two recent films, Red Road (2006) and The Unloved (2009)
The WiggleZ project: AAOmega and Dark Energy
We describe the `WiggleZ' spectroscopic survey of 280,000 star-forming
galaxies selected from a combination of GALEX ultra-violet and SDSS + RCS2
optical imaging. The fundamental goal is a detection of the baryonic acoustic
oscillations in galaxy clustering at high-redshift (0.5 < z < 1) and a precise
measurement of the equation of state of dark energy from this purely geometric
and robust method. The survey has already started on the 3.9m Anglo-Australian
Telescope using the AAOmega spectrograph, and planned to complete during 2009.
The WWW page for the survey can be found at
http://astronomy.swin.edu.au/wigglezComment: To appear in the Proceedings of the Durham "Cosmic Frontiers" ASP
conference eds. Metcalfe & Shanks, 8 pages, 5 figures, (Version 2: updated
May 2007 with final analysis of data through to Nov 2006.
The WiggleZ Dark Energy Survey: small-scale clustering of Lyman-break galaxies at z < 1
The WiggleZ Dark Energy Survey is a large-scale structure survey of intermediate-redshift ultraviolet-selected (UV-selected) emission-line galaxies scheduled to cover 1000 deg^2, spanning a broad redshift range 0.2 < z < 1.0 . The main scientific goal of the survey is the measurement of baryon acoustic oscillations (BAO) in the galaxy clustering pattern at a significantly higher redshift than previous studies. The BAO may be applied as a standard cosmological ruler to constrain dark energy models. Based on the first 20 per cent of the data set, we present initial results concerning the small-scale clustering of the WiggleZ targets, together with survey forecasts. The WiggleZ galaxy population possesses a clustering length r_0 = 4.40 ± 0.12 h^(−1) Mpc, which is significantly larger than z = 0 UV-selected samples, with a slope γ = 1.92 ± 0.08 . This clustering length is comparable to z = 3 Lyman-break galaxies with similar UV luminosities. The clustering strength of the sample increases with optical luminosity, UV luminosity and reddening rest-frame colour. The full survey, scheduled for completion in 2010, will map an effective volume V_eff ≈ 1 Gpc^3 (evaluated at a scale k = 0.15 h Mpc^(−1)) and will measure the angular diameter distance and Hubble expansion rates in three redshift bins with accuracies of ≈5 per cent. We will determine the value of a constant dark energy equation-of-state parameter, wcons, with a higher precision than existing supernovae observations using an entirely independent technique. The WiggleZ and supernova measurements lie in highly complementary directions in the plane of w_(cons) and the matter density Ω_m. The forecast using the full combination of WiggleZ, supernova and cosmic microwave background (CMB) data sets is a marginalized error Δw_(cons) = 0.07 , providing a robust and precise measurement of the properties of dark energy including cross-checking of systematic errors
Ichnological evidence for meiofaunal bilaterians from the terminal Ediacaran and earliest Cambrian of Brazil
The evolutionary events during the Ediacaran–Cambrian transition (~541 Myr ago) are unparalleled in Earth history. The fossil record suggests that most extant animal phyla appeared in a geologically brief interval, with the oldest unequivocal bilaterian body fossils found in the Early Cambrian. Molecular clocks and biomarkers provide independent estimates for the timing of animal origins, and both suggest a cryptic Neoproterozoic history for Metazoa that extends considerably beyond the Cambrian fossil record. We report an assemblage of ichnofossils from Ediacaran–Cambrian siltstones in Brazil, alongside U–Pb radioisotopic dates that constrain the age of the oldest specimens to 555–542 Myr. X-ray microtomography reveals three-dimensionally preserved traces ranging from 50 to 600 μm in diameter, indicative of small-bodied, meiofaunal tracemakers. Burrow morphologies suggest they were created by a nematoid-like organism that used undulating locomotion to move through the sediment. This assemblage demonstrates animal–sediment interactions in the latest Ediacaran period, and provides the oldest known fossil evidence for meiofaunal bilaterians. Our discovery highlights meiofaunal ichnofossils as a hitherto unexplored window for tracking animal evolution in deep time, and reveals that both meiofaunal and macrofaunal bilaterians began to explore infaunal niches during the late Ediacaran
The WiggleZ Dark Energy Survey: Survey Design and First Data Release
The WiggleZ Dark Energy Survey is a survey of 240,000 emission line galaxies
in the distant universe, measured with the AAOmega spectrograph on the 3.9-m
Anglo-Australian Telescope (AAT). The target galaxies are selected using
ultraviolet photometry from the GALEX satellite, with a flux limit of NUV<22.8
mag. The redshift range containing 90% of the galaxies is 0.2<z<1.0. The
primary aim of the survey is to precisely measure the scale of baryon acoustic
oscillations (BAO) imprinted on the spatial distribution of these galaxies at
look-back times of 4-8 Gyrs. Detailed forecasts indicate the survey will
measure the BAO scale to better than 2% and the tangential and radial acoustic
wave scales to approximately 3% and 5%, respectively.
This paper provides a detailed description of the survey and its design, as
well as the spectroscopic observations, data reduction, and redshift
measurement techniques employed. It also presents an analysis of the properties
of the target galaxies, including emission line diagnostics which show that
they are mostly extreme starburst galaxies, and Hubble Space Telescope images,
which show they contain a high fraction of interacting or distorted systems. In
conjunction with this paper, we make a public data release of data for the
first 100,000 galaxies measured for the project.Comment: Accepted by MNRAS; this has some figures in low resolution format.
Full resolution PDF version (7MB) available at
http://www.physics.uq.edu.au/people/mjd/pub/wigglez1.pdf The WiggleZ home
page is at http://wigglez.swin.edu.au
Discovery of a New Natural Product and a Deactivation of a Quorum Sensing System by Culturing a “Producer” Bacterium With a Heat-Killed “Inducer” Culture
Herein we describe a modified bacterial culture methodology as a tool to discover new natural products via supplementing actinomycete fermentation media with autoclaved cultures of “inducer” microbes. Using seven actinomycetes and four inducer microbes, we detected 28 metabolites that were induced in UHPLC-HRESIMS-based analysis of bacterial fermentations. Metabolomic analysis indicated that each inducer elicited a unique response from the actinomycetes and that some chemical responses were specific to each inducer-producer combination. Among these 28 metabolites, hydrazidomycin D, a new hydrazide-containing natural product was isolated from the pair Streptomyces sp. RKBH-B178 and Mycobacterium smegmatis. This result validated the effectiveness of the strategy in discovering new natural products. From the same set of induced metabolites, an in-depth investigation of a fermentation of Streptomyces sp. RKBH-B178 and autoclaved Pseudomonas aeruginosa led to the discovery of a glucuronidated analog of the pseudomonas quinolone signal (PQS). We demonstrated that RKBH-B178 is able to biotransform the P. aeruginosa quorum sensing molecules, 2-heptyl-4-quinolone (HHQ), and PQS to form PQS-GlcA. Further, PQS-GlcA was shown to have poor binding affinity to PqsR, the innate receptor of HHQ and PQS
CAMEMBERT: A Mini-Neptunes GCM Intercomparison, Protocol Version 1.0. A CUISINES Model Intercomparison Project
With an increased focus on the observing and modelling of mini-Neptunes,
there comes a need to better understand the tools we use to model their
atmospheres. In this paper, we present the protocol for the CAMEMBERT
(Comparing Atmospheric Models of Extrasolar Mini-neptunes Building and
Envisioning Retrievals and Transits) project, an intercomparison of general
circulation models (GCMs) used by the exoplanetary science community to
simulate the atmospheres of mini-Neptunes. We focus on two targets well studied
both observationally and theoretically with planned JWST Cycle 1 observations:
the warm GJ~1214b and the cooler K2-18b. For each target, we consider a
temperature-forced case, a clear sky dual-grey radiative transfer case, and a
clear sky multi band radiative transfer case, covering a range of complexities
and configurations where we know differences exist between GCMs in the
literature. This paper presents all the details necessary to participate in the
intercomparison, with the intention of presenting the results in future papers.
Currently, there are eight GCMs participating (ExoCAM, Exo-FMS, FMS PCM,
Generic PCM, MITgcm, RM-GCM, THOR, and the UM), and membership in the project
remains open. Those interested in participating are invited to contact the
authors.Comment: Accepted to PS
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