172 research outputs found
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Controlling a mobile robot with a biological brain
The intelligent controlling mechanism of a typical mobile robot is usually a computer system. Some recent research is ongoing in which biological neurons are being cultured and trained to act as the brain of an interactive real world robotïżœthereby either completely replacing, or operating in a cooperative fashion with, a computer system. Studying such hybrid systems can provide distinct insights into the operation of biological neural structures, and therefore, such research has immediate medical implications as well as enormous potential in robotics. The main aim of the research is to assess the computational and learning capacity of dissociated cultured neuronal networks. A hybrid system incorporating closed-loop control of a mobile robot by a dissociated culture of neurons has been created. The system is flexible and allows for closed-loop operation, either with hardware robot or its software simulation. The paper provides an overview of the problem area, gives an idea of the breadth of present ongoing research, establises a new system architecture and, as an example, reports on the results of conducted experiments with real-life robots
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Revealing ensemble state transition patterns in multi-electrode neuronal recordings using hidden Markov models
In order to harness the computational capacity of dissociated cultured neuronal networks, it is necessary to understand neuronal dynamics and connectivity on a mesoscopic scale. To this end, this paper uncovers dynamic spatiotemporal patterns emerging from electrically stimulated neuronal cultures using hidden Markov models (HMMs) to characterize multi-channel spike trains as a progression of patterns of underlying states of neuronal activity. However, experimentation aimed at optimal choice of parameters for such models is essential and results are reported in detail. Results derived from ensemble neuronal data revealed highly repeatable patterns of state transitions in the order of milliseconds in response to probing stimuli
Terahertz electrometry via infrared spectroscopy of atomic vapor
In recent years, the characterisation of radiation falling within the so-called âterahertz (THz) gapâ has become an ever more prominent issue due to the increasing use of THz systems in applications such as nondestructive testing, security screening, telecommunications, and medical diagnostics. THz detection technologies have advanced rapidly, yet traceable calibration of THz radiation remains challenging. In this paper, we demonstrate a system of electrometry in which a THz signal can be characterized using laser spectroscopy of highly excited (Rydberg) atomic states. We report on proof-of-principle measurements that reveal a minimum detectable THz electric field amplitude of 1.070.06 V/m at 1.06 THz (3 ms detection), corresponding to a THz power at the atomic cell of approximately 3.4 nW. Due to the relative simplicity and cryogen-free nature of this system, it has the potential to provide a route to a SI traceable âatomic candleâ for THz calibration across the terahertz frequency range, and provide an alternative to calorimetric methods
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Spatio-temporal dependencies in functional connectivity in rodent cortical cultures
Models of functional connectivity in cortical cultures on multi-electrodes arrays may aid in understanding how cognitive pathways form and improve techniques that aim to interface with neuronal systems. To enable research on such models, this study uses both data- and model-driven approaches to determine what dependencies are present in and between functional connectivity networks derived from bursts of extracellularly recorded activity. Properties of excitation in bursts were analysed using correlative techniques to assess the degree of linear dependence and then two parallel techniques were used to assess functional connectivity. Three models presenting increasing levels of spatio-temporal dependency were used to capture the dynamics of individual functional connections and their consistencies were verified using surrogate data. By comparing network-wide properties between model generated networks and functional networks from data, complex interdependencies were revealed. This indicates the persistent co-activation of neuronal pathways in spontaneous bursts, as can be found in whole brain structures
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Endogenous cholinergic tone modulates spontaneous network level neuronal activity in primary cortical cultures grown on multi-electrode arrays
Background
Cortical cultures grown long-term on multi-electrode arrays (MEAs) are frequently and extensively used as models of cortical networks in studies of neuronal firing activity, neuropharmacology, toxicology and mechanisms underlying synaptic plasticity. However, in contrast to the predominantly asynchronous neuronal firing activity exhibited by intact cortex, electrophysiological activity of mature cortical cultures is dominated by spontaneous epileptiform-like global burst events which hinders their effective use in network-level studies, particularly for neurally-controlled animat (âartificial animalâ) applications. Thus, the identification of culture features that can be exploited to produce neuronal activity more representative of that seen in vivo could increase the utility and relevance of studies that employ these preparations. Acetylcholine has a recognised neuromodulatory role affecting excitability, rhythmicity, plasticity and information flow in vivo although its endogenous production by cortical cultures and subsequent functional influence upon neuronal excitability remains unknown.
Results
Consequently, using MEA electrophysiological recording supported by immunohistochemical and RT-qPCR methods, we demonstrate for the first time, the presence of intrinsic cholinergic neurons and significant, endogenous cholinergic tone in cortical cultures with a characterisation of the muscarinic and nicotinic components that underlie modulation of spontaneous neuronal activity. We found that tonic muscarinic ACh receptor (mAChR) activation affects global excitability and burst event regularity in a culture age-dependent manner whilst, in contrast, tonic nicotinic ACh receptor (nAChR) activation can modulate burst duration and the proportion of spikes occurring within bursts in a spatio-temporal fashion.
Conclusions
We suggest that the presence of significant endogenous cholinergic tone in cortical cultures and the comparability of its modulatory effects to those seen in intact brain tissues support emerging, exploitable commonalities between in vivo and in vitro preparations. We conclude that experimental manipulation of endogenous cholinergic tone could offer a novel opportunity to improve the use of cortical cultures for studies of network-level mechanisms in a manner that remains largely consistent with its functional role
Compact HI clouds from the GALFA-HI survey
The Galactic Arecibo L-band Feed Array HI (GALFA-HI) survey is mapping the
entire Arecibo sky at 21-cm, over a velocity range of -700 to +700 km/s (LSR),
at a velocity resolution of 0.18 km/s and a spatial resolution of 3.5 arcmin.
The unprecedented resolution and sensitivity of the GALFA-HI survey have
resulted in the detection of numerous isolated, very compact HI clouds at low
Galactic velocities, which are distinctly separated from the HI disk emission.
In the limited area of ~4600 deg surveyed so far, we have detected 96 of
such compact clouds. The detected clouds are cold with a median T
(the kinetic temperature in the case in which there is no non-thermal
broadening) of 300 K. Moreover, these clouds are quite compact and faint, with
median values of 5 arcmin in angular size, 0.75 K in peak brightness
temperature, and cm in HI column density. Most of the
clouds deviate from Galactic rotation at the 20-30 km/s level, and a
significant fraction show evidence for a multiphase medium and velocity
gradients. No counterparts for these clouds were found in other wavebands. From
the modeling of spatial and velocity distributions of the whole compact cloud
population, we find that the bulk of the compact clouds are related to the
Galactic disk, and their distances are likely to be in the range of 0.1 to a
few kpc. We discuss various possible scenarios for the formation and
maintenance of this cloud population and its significance for Galactic ISM
studies.Comment: Accepted for publication in the Astrophysical Journa
The APOGEE-2 Survey of the Orion Star Forming Complex: I. Target Selection and Validation with early observations
The Orion Star Forming Complex (OSFC) is a central target for the APOGEE-2
Young Cluster Survey. Existing membership catalogs span limited portions of the
OSFC, reflecting the difficulty of selecting targets homogeneously across this
extended, highly structured region. We have used data from wide field
photometric surveys to produce a less biased parent sample of young stellar
objects (YSOs) with infrared (IR) excesses indicative of warm circumstellar
material or photometric variability at optical wavelengths across the full 420
square degrees extent of the OSFC. When restricted to YSO candidates with H <
12.4, to ensure S/N ~100 for a six visit source, this uniformly selected sample
includes 1307 IR excess sources selected using criteria vetted by Koenig &
Liesawitz and 990 optical variables identified in the Pan-STARRS1 3
survey: 319 sources exhibit both optical variability and evidence of
circumstellar disks through IR excess. Objects from this uniformly selected
sample received the highest priority for targeting, but required fewer than
half of the fibers on each APOGEE-2 plate. We fill the remaining fibers with
previously confirmed and new color-magnitude selected candidate OSFC members.
Radial velocity measurements from APOGEE-1 and new APOGEE-2 observations taken
in the survey's first year indicate that ~90% of the uniformly selected targets
have radial velocities consistent with Orion membership.The APOGEE-2 Orion
survey will include >1100 bona fide YSOs whose uniform selection function will
provide a robust sample for comparative analyses of the stellar populations and
properties across all sub-regions of Orion.Comment: Accepted for publication in ApJ
The Fourteenth Data Release of the Sloan Digital Sky Survey: First Spectroscopic Data from the extended Baryon Oscillation Spectroscopic Survey and from the second phase of the Apache Point Observatory Galactic Evolution Experiment
The fourth generation of the Sloan Digital Sky Survey (SDSS-IV) has been in
operation since July 2014. This paper describes the second data release from
this phase, and the fourteenth from SDSS overall (making this, Data Release
Fourteen or DR14). This release makes public data taken by SDSS-IV in its first
two years of operation (July 2014-2016). Like all previous SDSS releases, DR14
is cumulative, including the most recent reductions and calibrations of all
data taken by SDSS since the first phase began operations in 2000. New in DR14
is the first public release of data from the extended Baryon Oscillation
Spectroscopic Survey (eBOSS); the first data from the second phase of the
Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE-2),
including stellar parameter estimates from an innovative data driven machine
learning algorithm known as "The Cannon"; and almost twice as many data cubes
from the Mapping Nearby Galaxies at APO (MaNGA) survey as were in the previous
release (N = 2812 in total). This paper describes the location and format of
the publicly available data from SDSS-IV surveys. We provide references to the
important technical papers describing how these data have been taken (both
targeting and observation details) and processed for scientific use. The SDSS
website (www.sdss.org) has been updated for this release, and provides links to
data downloads, as well as tutorials and examples of data use. SDSS-IV is
planning to continue to collect astronomical data until 2020, and will be
followed by SDSS-V.Comment: SDSS-IV collaboration alphabetical author data release paper. DR14
happened on 31st July 2017. 19 pages, 5 figures. Accepted by ApJS on 28th Nov
2017 (this is the "post-print" and "post-proofs" version; minor corrections
only from v1, and most of errors found in proofs corrected
Topoisomerase IIα Binding Domains of Adenomatous Polyposis Coli Influence Cell Cycle Progression and Aneuploidy
Truncating mutations in the tumor suppressor gene APC (Adenomatous Polyposis Coli) are thought to initiate the majority of colorectal cancers. The 15- and 20-amino acid repeat regions of APC bind beta-catenin and have been widely studied for their role in the negative regulation of canonical Wnt signaling. However, functions of APC in other important cellular processes, such as cell cycle control or aneuploidy, are only beginning to be studied. Our previous investigation implicated the 15-amino acid repeat region of APC (M2-APC) in the regulation of the G2/M cell cycle transition through interaction with topoisomerase IIalpha (topo IIalpha).We now demonstrate that the 20-amino acid repeat region of APC (M3-APC) also interacts with topo IIalpha in colonic epithelial cells. Expression of M3-APC in cells with full-length endogenous APC causes cell accumulation in G2. However, cells with a mutated topo IIalpha isoform and lacking topo IIbeta did not arrest, suggesting that the cellular consequence of M2- or M3-APC expression depends on functional topoisomerase II. Both purified recombinant M2- and M3-APC significantly enhanced the activity of topo IIalpha. Of note, although M3-APC can bind beta-catenin, the G2 arrest did not correlate with beta-catenin expression or activity, similar to what was seen with M2-APC. More importantly, expression of either M2- or M3-APC also led to increased aneuploidy in cells with full-length endogenous APC but not in cells with truncated endogenous APC that includes the M2-APC region.Together, our data establish that the 20-amino acid repeat region of APC interacts with topo IIalpha to enhance its activity in vitro, and leads to G2 cell cycle accumulation and aneuploidy when expressed in cells containing full-length APC. These findings provide an additional explanation for the aneuploidy associated with many colon cancers that possess truncated APC
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