Anestesia intradural con mepivacaína hiperbárica a dosis bajas en la cirugía ambulatoria de la hernia inguinal

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Medicina, Departamento de Cirugía. Fecha de lectura: 5-07-201

Integrated analysis of root microbiomes of soybean and wheat from agricultural fields

Root associated bacteria are critical for plant growth and health. Understanding the composition and role of root microbiota is crucial toward agricultural practices that are less dependent on chemical fertilization, which has known negative effects on the environment and human health. Here we analyzed the root-associated microbiomes of soybean and wheat under agricultural field conditions. We took samples from 11 different production fields across a large geographic area. We used 16S rRNA pyrosequencing to explore root microbial communities and also obtained 2,007 bacterial isolates from rhizospheres, which were tested for the presence of plant growth promoting (PGP) traits in-vitro. We observed that pH and nitrate content correlated with beta diversity variability of rhizospheric bacterial communities despite the variable field conditions. We described the dominant bacterial groups associated to roots from both crops at a large geographic scale and we found that a high proportion of them (60-70%) showed more than 97% similarity to bacteria from the isolated collection. Moreover, we observed that 55% of the screened isolates presented PGP activities in vitro. These results are a significant step forward in understanding crop-associated microbiomes and suggest that new directions can be taken to promote crop growth and health by modulating root microbiomes.Fil: Rascovan, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Carbonetto, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: Perrig, Diego Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Rizobacter Argentina S. A.; ArgentinaFil: Diaz, Marisa. Rizobacter Argentina S. A.; ArgentinaFil: Canciani, Wilter. Rizobacter Argentina S. A.; ArgentinaFil: Abalo, Matías. Rizobacter Argentina S. A.; ArgentinaFil: Alloati, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; ArgentinaFil: González Anta, Gustavo. Rizobacter Argentina S. A.; ArgentinaFil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentin

Evaluación del confort acústico en distintos ambientes

El objetivo principal de este proyecto es estudiar cómo perciben los usuarios el confort acústico en distintos ambientes. Para ello se utilizó un índice que tendrá en cuenta los diferentes factores que influyen en la percepción de la molestia de cada persona. Además del objetivo principal, hay otros objetivos secundarios que han de completarse para llegar a una conclusión final. Los objetivos secundarios son los siguientes: 1) Estudiar los factores acústicos que influyen en la percepción de la molestia. 2) Medir el tiempo de reverberación de cada sala. 3) Llevar a cabo un estudio de campo a los usuarios en cada ambiente mediante una encuesta. 4) Medir el nivel de presión sonora y el índice IRO y relacionarlos con el estudio de campo realizado. Una vez cumplidos todos los objetivos secundarios se llegará a una conclusión final para proponer algún tipo de mejora en el método utilizado para evaluar el confort acústico.Departamento de Física AplicadaIngeniero Técnico Industrial, Especialidad en Electrónica Industria

Is Marathon Training Harder than the Ironman Training? An ECO-method Comparison

Purpose: To compare the absolute and relative training load of the Marathon (42k) and the Ironman (IM) training in recreational trained athletes. Methods: Fifteen Marathoners and Fifteen Triathletes participated in the study. Their performance level was the same relative to the sex's absolute winner at the race. No differences were presented neither in age, nor in body weight, height, BMI, running VO2max max, or endurance training experience (p > 0.05). They all trained systematically for their respective event (IM or 42k). Daily training load was recorded in a training log, and the last 16 weeks were compared. Before this, gas exchange and lactate metabolic tests were conducted in order to set individual training zones. The Objective Load Scale (ECOs) training load quantification method was applied. Differences between IM and 42k athletes' outcomes were assessed using Student's test and significance level was set at p < 0.05. Results: As expected, Competition Time was significantly different (IM 11 h 45 min ± 1 h 54 min vs. 42k 3 h 6 min ± 28 min, p < 0.001). Similarly, Training Weekly Avg Time (IM 12.9 h ± 2.6 vs. 42k 5.2 ± 0.9), and Average Weekly ECOs (IM 834 ± 171 vs. 42k 526 ± 118) were significantly higher in IM (p < 0.001). However, the Ratio between Training Load and Training Time was superior for 42k runners when comparing ECOs (IM 65.8 ± 11.8 vs. 42k 99.3 ± 6.8) (p < 0.001). Finally, all ratios between training time or load vs. Competition Time were superior for 42k (p < 0.001) (Training Time/Race Time: IM 1.1 ± 0.3 vs. 42k 1.7 ± 0.5), (ECOs Training Load/Race Time: IM 1.2 ± 0.3 vs. 42k 2.9 ± 1.0). Conclusions: In spite of IM athletes' superior training time and total or weekly training load, when comparing the ratios between training load and training time, and training time or training load vs. competition time, the preparation of a 42k showed to be harder.Sin financiación3.394 JCR (2017) Q1, 20/83 Physiology1.590 SJR (2017) Q1, 41/191 Physiology, 23/107 Physiology (medical)No data IDR 2017UE

Transpiration Dynamics of Esparto Grass (<i>Macrochloa tenacissima</i> (L.) Kunth) in a Semi-Arid Mediterranean Climate: Unraveling the Impacts of Pine Competition

Macrochloa tenacissima (M. tenacissima), or esparto, is a perennial tussock grass that coexists with Pinus halepensis (P. halepensis) in semi-arid Mediterranean woodlands. This research was carried out to explore diurnal transpiration at leaf level in esparto grass under different levels of pine–esparto competition and in contrasting environmental soil water conditions. The measurement period spanned from the summer of 2020 to the spring of 2021. The relationship between transpiration and competition was conducted in open and closed P. halepensis stands, and the type of leaf (green, senescent) and the maturity of the esparto grass were taken into account. We observed a higher control of transpiration in green leaves, and the correlations between the transpiration and pine competition were noted exclusively in this type of leaf. Our results demonstrated a significant impact of pine competitors (closed stands) on the transpiration of esparto grass, particularly during seasons characterized by scenarios of high water demand: the summer drought period and the commencement of the growing and flowering period (spring). Furthermore, our findings revealed a greater response to transpiration in mature bushes compared to young ones under severe water stress, indicating a higher adaptation to drought by esparto as it ages. Although our results confirmed that PAR increased transpiration in all seasons and in both stands, which is attributable to the heliophilia of esparto grass, the site effects on transpiration could also be attributable to competition for water, especially during periods of drought. These results may have important implications for the dynamics and management of these semi-arid mixed woodlands, as well as the planning of reforestation programs aimed at restoring esparto grass formations

Cultivos de cobertura y bajas dosis de fertilizantes nitrogenados: efecto sobre la funcionalidad del suelo en el largo plazo

La estructura del suelo junto con los contenidos de carbono y nitrógeno son atributos críticos que condicionan el funcionamiento edáfico y promueven la resiliencia y sustentabilidad de los agroecosistemas. La glomalina, glicoproteína liberada por hongos micorrícicos arbusculares, es un factor importante en la estabilización de agregados y secuestro de carbono. En sistemas de bajos insumos, estos atributos podrían mejorar con el uso de cultivos de cobertura (CC) en el largo plazo. El objetivo del trabajo fue evaluar el efecto de incluir CC a una rotación soja-maíz combinada con baja o nula fertilización nitrogenada sobre el contenido de glomalinas, carbono y nitrógeno orgánico del suelo (COS y NOS, respectivamente), carbono orgánico particulado (COP) y estabilidad estructural (EE) luego de diez años.EEA PergaminoFil: Restovich, Silvina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Suelos; Argentina.Fil: Portela, Silvina Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio de Suelos; Argentina.Fil: Farroni, Abel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, suelo y Agua; Argentina. Universidad Nacional del Noroeste de Buenos Aires (UNNOBA). Escuela de Ciencias Agrarias, Naturales y Ambientales (ECANA); Argentina.Fil: García Prisi, Pablo. Universidad Nacional del Noroeste de Buenos Aires (UNNOBA). Centro de Investigaciones y Transferencias del Noroeste de Buenos Aires (CITNOBA). Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.Fil: García, Leticia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, suelo y Agua; Argentina.Fil: Copia, Pablo Antonio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Laboratorio Calidad de Alimento, suelo y Agua; Argentina.Fil: Chavarria, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Patología Vegetal (IPAVE-CIAP, INTA); Argentina.Fil: Gonzalez Anta, Gustavo. Universidad Nacional del Noroeste de Buenos Aires (UNNOBA). Escuela de Ciencias Agrarias, Naturales y Ambientales (ECANA); Argentina.Fil: Albarracín, Daniela. Universidad Nacional del Noroeste de Buenos Aires (UNNOBA). Escuela de Ciencias Agrarias, Naturales y Ambientales (ECANA); Argentina

Creating pathways for collaboration between Argentina and the UK to utilise microbial biofilms in sustainable agriculture

Microbial biofilms are complex communities of microorganisms that exist in various environments, including natural and human-built systems and have a significant economic impact on a global scale. In addition to their detrimental impacts, biofilms have been extensively studied for their potential benefits. In agriculture, biofilms are considered key organisational structures of microbes, exploited as biofertilisers, biostimulants, and biocontrol agents, with the potential to enhance soil health and plant growth. Despite ongoing research, there are still large knowledge gaps in the understanding of the mechanisms governing interaction between biofilms and plants, and how these can be manipulated to promote sustainable agriculture. The UK’s National Biofilms Innovation Centre [NBIC] and Argentine researchers have established a dialogue aimed at addressing these gaps and improving agricultural productivity through the integration of new technologies that can promote soil health whilst reducing environmental impact. Future research collaborations between the two countries in this area could have significant benefits for global agricultural innovation and the development of sustainable food systems. This publication takes on a ‘white paper’ format, consolidating complex discussions from a workshop between NBIC and Argentine researchers. It offers a comprehensive summary encompassing the insights, perspectives, and outcomes generated during the discussions among the participants, pinpointing three key priority areas for collaborative activities that were identified: (1) Using plant root biofilm composition as a sensor for soil health and to optimise interventions, (2) Biofilms and soil health resilience in a changing environment, (3) Intelligent seeds and innovative / automated large-scale monitoring systems. For the three identified priority areas, the early engagement of end-users [farmers] will be paramount to maximise technology adoption. Commitment from the governments and support from funding bodies in both countries will be essential for the establishment of robust research programmes and long-term successful collaborations between researchers, industry and end users. Graphical Abstract: (Figure presented.)</p