14 research outputs found
Interstitial cell migration: integrin-dependent and alternative adhesion mechanisms
Adhesion and migration are integrated cell functions that build, maintain and remodel the multicellular organism. In migrating cells, integrins are the main transmembrane receptors that provide dynamic interactions between extracellular ligands and actin cytoskeleton and signalling machineries. In parallel to integrins, other adhesion systems mediate adhesion and cytoskeletal coupling to the extracellular matrix (ECM). These include multifunctional cell surface receptors (syndecans and CD44) and discoidin domain receptors, which together coordinate ligand binding with direct or indirect cytoskeletal coupling and intracellular signalling. We review the way that the different adhesion systems for ECM components impact cell migration in two- and three-dimensional migration models. We further discuss the hierarchy of these concurrent adhesion systems, their specific tasks in cell migration and their contribution to migration in three-dimensional multi-ligand tissue environments
Natural and anthropogenic nitrogen uptake by bloom-forming macroalgae
The frequency and duration of macroalgal blooms have increased in many coastal waters over the past several decades. We used field surveys and laboratory culturing experiments to examine the nitrogen content and Ξ΄ 15N values of Ulva and Gracilaria, two bloom-forming algal genera in Narragansett Bay, RI (USA). The northern end of this bay is densely populated with large sewage treatment plant nitrogen inputs; the southern end is more lightly populated and opens to the Atlantic Ocean. Field-collected Ulva varied in Ξ΄ 15N among sites, but with two exceptions had Ξ΄ 15N above 10β°, reflecting a significant component of heavy anthropogenic N. This variation was not correlated with a north-south gradient. Both Ulva and Gracilaria cultured in water from across Narragansett Bay also had high signals (Ξ΄ 15N = βΌ14-17β° and 8-12β°, respectively). These results indicate that inputs of anthropogenic N can have far-reaching impacts throughout estuaries. Β© 2007 Elsevier Ltd. All rights reserved
Distribution and trophic importance of anthropogenic nitrogen in Narragansett Bay: An assessment using stable isotopes
Narragansett Bay has been heavily influenced by human activities for more than 200 years. In recent decades, it has been one of the more intensively fertilized estuaries in the USA, with most of the anthropogenic nutrient load originating from sewage treatment plants (STP). This will soon change as tertiary treatment upgrades reduce nitrogen (N) loads by about one third or more during the summer. Before these reductions take place, we sought to characterize the sewage N signature in primary (macroalgae) and secondary (the hard clam, Mercenaria mercenaria) producers in the bay using stable isotopes of N (Ξ΄15N) and carbon (Ξ΄13C). The Ξ΄15N signatures of the macroalgae show a clear gradient of approximately 4β° from north to south, i.e., high to low point source loading. There is also evidence of a west to east gradient of heavy to light values of Ξ΄15N in the bay consistent with circulation patterns and residual flows. The Providence River Estuary, just north of Narragansett Bay proper, receives 85% of STP inputs to Narragansett Bay, and lower Ξ΄15N values in macroalgae there reflected preferential uptake of 14N in this heavily fertilized area. Differences in pH from N stimulated photosynthesis and related shifts in predominance of dissolved C species may control the observed Ξ΄13C signatures. Unlike the macroalgae, the clams were remarkably uniform in both Ξ΄15N (13.2Β±0.54β° SD) and Ξ΄13C (-16.76Β±0. 61β° SD) throughout the bay, and the Ξ΄15N values were 2-5β° heavier than in clams collected outside the bay. We suggest that this remarkable uniformity reflects a food source of anthropogenically heavy phytoplankton formed in the upper bay and supported by sewage derived N. We estimate that approximately half of the N in the clams throughout Narragansett Bay may be from anthropogenic sources. Β© 2007 Coastal and Estuarine Research Federation
Poly(ethylene glycol) enhances cell motility on protein-based poly(ethylene glycol)-polycarbonate substrates: A mechanism for cell-guided ligand remodeling
Directing cell migration and organization via nanocrater-patterned cell-repellent interfaces
Although adhesive interactions between cells and nanostructured interfaces have been studied extensively(1β6), there is a paucity of data on how nanostructured interfaces repel cells by directing cell migration and cell-colony organization. Here, by using multiphoton ablation lithography(7) to pattern surfaces with nanoscale craters of various aspect ratios and pitches, we show that the surfaces altered the cellsβ focal-adhesion size and distribution, thus affecting cell morphology, migration and ultimately localization. We also show that nanocrater pitch can disrupt the formation of mature focal adhesions to favour the migration of cells toward higher-pitched regions, which present increased planar area for the formation of stable focal adhesions. Moreover, by designing surfaces with variable pitch but constant nanocrater dimensions, we were able to create circular and striped cellular patterns. Our surface-patterning approach, which does not involve chemical treatments and can be applied to various materials, represents a simple method to control cell behaviour on surfaces