Proses dan Peran Reporter Dalam Penulisan Berita Media Online HASNA.ID

Memasuki era digital dengan teknologi yang semakin canggih dan memudahkan kehidupan masyarakat juga menuntut perusahaan media melakukan perubahan mengikuti perkembangan zaman saat ini. Proses peralihan yang berlangsung ini memungkinkan media massa memanfaatkan teknologi-teknologi yang telah tersedia dalam mengkomunikasikan informasi kepada khalayak. Untuk itu, diperlukan adanya kreativitas dari para pekerja media untuk memproduksi konten yang faktual dan informatif. Munculnya platform media online atau jurnalisme online merupakan salah satu bentuk konvergensi media yang membawa perubahan sosial di tengah masyarakat. Untuk itu, pembaca berita daring (online) di Indonesia juga terus meningkat pada 2017 sebesar 35,8 persen dibandingkan 2015 yang disesuaikan dengan jumlah pengguna internet serta diyakini bersifat praktis, tidak memerlukan biaya, dan dapat diakses kapan saja (timeless). Kehadiran media online juga membangun interaksi dua arah antara praktisi media dan pembaca sehingga mempermudah dalam pemilihan konten yang ingin dikonsumsi. Hal serupa dilakukan oleh Hasna.ID yang merupakan media digital di bawah naungan Hasna Media, PT Hasna Global Media yang menyajikan berita yang fokus pada, lifestyle, gaya berpakaian (fashion), kecantikan (beauty), kesehatan (health) dan ditujukan pada kaum milenial khususnya perempuan usia 17 hingga 35 tahun

Pemanfaatan Media Sosial sebagai Sumber Berita oleh Media Online Detikcom dan Tempo.co

Oleh : Gracia Davina Wu Lahirnya fenomena mengikuti isu-isu yang viral atau trending topic di media sosial rawan berisikan misinformasi dan disinformasi sehingga tidak menutup kemungkinan bahwa praktisi media atau jurnalis juga dapat terjebak pada isi dari informasi tersebut sehingga diperlukan adanya aktivitas pemeriksaan fakta secara mendalam agar mampu memberikan klarifikasi secara akurat dan menjawab ketidakpastian dari informasi yang beredar sehingga masyarakat tidak terjebak pada berita bohong. Tujuan dari penelitian ini untuk mengetahui media sosial apa saja yang digunakan, untuk mengetahui klaim atau isu yang dipilih dan ditelusuri faktanya, untuk mengetahui praktik pengecekan faktayang diterapkan hingga dipublikasikan kepada khalayak serta tools yang dimanfaatkan dalam menelusuri konten multimedia dari media sosial. Konsep utama yang digunakan yaitu dari First Draft News. Metode penelitian berupa kualitatif deskriptif dan proses pengumpulan data dilakukan dengan wawancara mendalam. Dari hasil penelitian dapat diperoleh bahwa dalam aspek pemilihan isu yang akan ditelusuri harus mengandung unsur viralitas, penting dan informatif, urgent untuk segera diberitakan, dan berdampak besar bagi publik. Dalam praktik pengecekan fakta, Detikcom menelusuri terkait lokasi (location), waktu (date) dan keaslian sumber (provenance) berdasarkan pernyataan narasumber dan penemuan di lokasi peristiwa. Sedangkan, cek fakta Tempo.co melakukan penelusuran fakta hampir serupa dengan konsep milik First Draft News yaitu asal usul konten, sumber, lokasi dan waktu. Meskipun tim cek fakta Tempo.co memiliki model pengecekan faktayang baik namun belum dilakukan secara maksimal karena tidak menelusuri keaslian konten (provenance), waktu konten diambil pertama kali (date) dan motivasi pemilik konten (motivation)

First Steps towards Parameter Optimization of Bioelectrochemical Systems using a Microsystems Engineering Approach

There is a growing interest in exploiting bioelectrochemical systems (BESs), such as microbial fuel cells, as an alternative energy source for sustainable living. Certain species of microorganisms, such as Pseudomonas aeruginosa 14 (PA14) wild type, produce electron carriers, Phenazines, which transfers electrons to the anode in the system and produce digital output signal. The electric current generation of BESs is influenced by many biophysical and biochemical parameters in the system, such as glucose level, cell culture community, cell density, PH, and oxygen level. The existing MFCs are at macroscale, and not suitable for parameter optimization; and as a result they are not yet cost effective. Here, we present our first effort in parameter optimization of BESs using a microfluidic device. Microfluidic device affords us an ability to quickly define a physical and chemical environment for PA14, and its compatibility with microscope allows us a real time observation of the responses. We will present experimental results on the roles of carbon sources in PA14 motility and promising results of Phenazines being a chemoattractant to PA14. We will discuss the relation between PA14 motility and biofilm formation, and subsequently electric current generation

Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates

<p>Abstract</p> <p>Background</p> <p>Conditional knockout mice are a useful tool to study the function of gene products in a tissue-specific or inducible manner. Classical approaches to generate targeting vectors for conditional alleles are often limited by the availability of suitable restriction sites. Furthermore, plasmid-based targeting vectors can only cover a few kB of DNA which precludes the generation of targeting vectors where the two <it>lox</it>P sites are placed far apart. These limitations have been overcome in the recent past by using homologous recombination of bacterial artificial chromosomes (BACs) in <it>Escherichia coli </it>to produce large targeting vector containing two different <it>lox</it>P-flanked selection cassettes so that a single targeting event is sufficient to introduce <it>lox</it>P-sites a great distances into the mouse genome. However, the final targeted allele should be free of selection cassettes and screening for correct removal of selection cassettes can be a laborious task. Therefore, we developed a new strategy to rapidly identify ES cells containing the desired allele.</p> <p>Results</p> <p>Using BAC recombineering we generated a single targeting vector which contained two different selection cassettes that were flanked by <it>lox</it>P-<it>lox</it>P sites or by FRT-FRT/<it>lox</it>P sites so that they could be deleted sequentially by Cre- and FLPe-recombinases, respectively. Transfected ES cells were first selected in the presence of both antibiotics <it>in vitro </it>before correctly targeted clones were identified by Southern blot. After transfection of a Cre recombinase expression plasmid ES cell clones were selected on replicate plates to identify those clones which maintained the FRT-FRT/<it>lox</it>P flanked cassette and lost the <it>lox</it>P-<it>lox</it>P flanked cassette. Using this strategy facilitated the identification of ES cell clones containing the desired allele before blastocyst injection.</p> <p>Conclusion</p> <p>The strategy of ES cell cultures in replicate plates proved to be very efficient in identifying ES cells that had undergone the correct recombination event. This approach facilitates the generation of conditional knock-out mice when large parts of the genome are intended to be flanked by <it>lox</it>P sites.</p

Effects of dietary macronutrients on the hepatic transcriptome and serum metabolome in mice

ACKNOWLEDGMENTS The study was funded by National Key R&D Program of China (2019YFA0801900), National Postdoctoral Program for Innovative Talents (BX2021357), Postdoctoral Science Foundation (2021M693393) the Chinese Academy of Sciences Strategic Program (XDB13030100), the National Natural Science Foundation of China (91649108), a PIFI professorial fellowship from CAS and a Wolfson merit award from the UK Royal Society (all to J.R.S unless stated).Peer reviewedPublisher PD

Identification and quantification of anthocyanins in transgenic purple tomato

Citation: Su, X., Xu, J., Rhodes, D., Shen, Y., Song, W., Katz, B., … Wang, W. (2016). Identification and quantification of anthocyanins in transgenic purple tomato. Food Chemistry, 202, 184–188. https://doi.org/10.1016/j.foodchem.2016.01.128Anthocyanins are natural pigments derived from the phenylpropanoid pathway. Most tomatoes produce little anthocyanins, but the transgenic purple tomato biosynthesizes a high level of anthocyanins due to expression of two transcription factors (Del and Ros1). This study was to identify and quantify anthocyanins in this transgenic tomato line. Seven anthocyanins, including two new anthocyanins [malvidin-3-(p-coumaroyl)-rutinoside-5-glucoside and malvidin-3-(feruloyl)-rutinoside-5-glucoside], were identified by LC-MS/MS. Petunidin-3-(trans-coumaroyl)-rutinoside-5-glucoside and delphinidin-3-(trans-coumaroyl)-rutinoside-5-glucoside were the most abundant anthocyanins, making up 86% of the total anthocyanins. Compared to undetectable anthocyanins in the wild type, the contents of anthocyanins in the whole fruit, peel, and flesh of the Del/Ros1-transgenic tomato were 5.2 ± 0.5, 5.1 ± 0.5, and 5.8 ± 0.3 g/kg dry matter, respectively. Anthocyanins were undetectable in the seeds of both wide-type and transgenic tomato lines. Such novel and high levels of anthocyanins obtained in this transgenic tomato may provide unique functional products with potential health benefits

Construction of an almond linkage map in an Australian population Nonpareil × Lauranne

Background: Despite a high genetic similarity to peach, almonds (Prunus dulcis) have a fleshless fruit and edible kernel, produced as a crop for human consumption. While the release of peach genome v1.0 provides an excellent opportunity for almond genetic and genomic studies, well-assessed segregating populations and the respective saturated genetic linkage maps lay the foundation for such studies to be completed in almond. Results: Using an almond intraspecific cross between ‘Nonpareil’ and ‘Lauranne’ (N × L), we constructed a moderately saturated map with SSRs, SNPs, ISSRs and RAPDs. The N × L map covered 591.4 cM of the genome with 157 loci. The average marker distance of the map was 4.0 cM. The map displayed high synteny and colinearity with the Prunus T × E reference map in all eight linkage groups (G1-G8). The positions of 14 mapped gene-anchored SNPs corresponded approximately with the positions of homologous sequences in the peach genome v1.0. Analysis of Mendelian segregation ratios showed that 17.9% of markers had significantly skewed genotype ratios at the level of P < 0.05. Due to the large number of skewed markers in the linkage group 7, the potential existence of deleterious gene(s) was assessed in the group. Integrated maps produced by two different mapping methods using JoinMap® 3 were compared, and their high degree of similarity was evident despite the positional inconsistency of a few markers. Conclusions: We presented a moderately saturated Australian almond map, which is highly syntenic and collinear with the Prunus reference map and peach genome V1.0. Therefore, the well-assessed almond population reported here can be used to investigate the traits of interest under Australian growing conditions, and provides more information on the almond genome for the international community.Iraj Tavassolian, Gholmereza Rabiei, Davina Gregory, Mourad Mnejja, Michelle G Wirthensohn, Peter W Hunt, John P Gibson, Christopher M Ford, Margaret Sedgley, and Shu-Biao W

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P &lt; 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Hepatic progenitor cells of biliary origin with liver repopulation capacity

Hepatocytes and cholangiocytes self-renew following liver injury. Following severe injury hepatocytes are increasingly senescent, but whether hepatic progenitor cells (HPCs) then contribute to liver regeneration is unclear. Here, we describe a mouse model where the E3 ubiquitin ligase Mdm2 is inducibly deleted in more than 98% of hepatocytes, causing apoptosis, necrosis and senescence with nearly all hepatocytes expressing p21. This results in florid HPC activation, which is necessary for survival, followed by complete, functional liver reconstitution. HPCs isolated from genetically normal mice, using cell surface markers, were highly expandable and phenotypically stable in vitro. These HPCs were transplanted into adult mouse livers where hepatocyte Mdm2 was repeatedly deleted, creating a non-competitive repopulation assay. Transplanted HPCs contributed significantly to restoration of liver parenchyma, regenerating hepatocytes and biliary epithelia, highlighting their in vivo lineage potency. HPCs are therefore a potential future alternative to hepatocyte or liver transplantation for liver disease

Type 2 Cytokines in Immunity and Metabolic Regulation

In metazoan organisms ranging from simple to the complex, the innate immune system is the first line of defense against damage or infection. Vertebrates have the particular challenge of dealing with parasitism and allergen exposure, to which Type 2 responses are critical at mucosal barrier sites. While there is some overlap between innate and adaptive Type 2 effector cell functions, their unique contributions are unclear. Additionally, the broader role of innate immunity in tissue regulation as it relates to the conserved mechanisms of metabolic homeostasis is unexplored. We targeted the IL-4/13 locus in mice to generate a Th2-specific model of IL-4/13 deficiency (CD4Cre x IL-4/13 fl/fl). Consistent with prior studies, we verified that CD4 T cell-derived IL-4/13 was not required for the clearance of acute Nippostrongylus brasiliensis helminth infection. However, in response to chronic conditions, such as OVA-induced airway inflammation and Schistosoma mansoni infection, conditional knockout mice had an intermediate phenotype. The conditional CD4Cre x IL-4/13 fl/fl model suggests that while innate IL-4/13 can mediate the clearance of acute helminth infection, they may interact with Th2 cells in chronic inflammation. As IL-4/13 have been implicated as important for adipose tissue macrophage alternative activation in the lean metabolic state, we identified eosinophils as the major IL-4-producing cells in white adipose tissue. In eosinophil-deficient mice, adipose tissue macrophage alternative activation was impaired but could be restored by reconstituting the eosinophil lineage. Additionally, eosinophil-deficient mice, when maintained on a high fat diet, developed increased adiposity and glucose intolerance. Thus, we find that eosinophils play a role in metabolic regulation by sustaining alternatively activated macrophages that promote the lean phenotype. Our findings confirm that Type 2 innate effector cells are important for the clearance of acute helminth infection, but may be interacting with Th2 cells during chronic infection and airway inflammation. We have also uncovered a surprising observation that eosinophils, a major feature of Type 2 immune responses, sustain macrophage alternative activation in adipose tissue. This suggests that innate cells have evolved functions not only for immunity, but for also for metabolic homeostasis as part of a highly integrated survival strategy