Evaluation of a field-test kit for triazine herbicides (SensioScreen® TR500) as a fast assay to detect pesticide contamination in water samples

11 pages, 3 figures, 7 tables.-- Available online Nov 16, 2002.A field-test kit (SensioScreen® TR500) for the determination of triazine herbicides in water samples has been evaluated. The test is based on an ELISA method performed on a membrane that allows the visual estimation of the presence of triazine herbicides in <10 min. The kit contains all necessary reagents and equipment, and operates on a very simple protocol. The detection level of this semiquantitative kit has been set at 0.5 μg/l (for the sum of all triazines), although concentration levels of 0.1 μg/l for atrazine can be detected. This is the first time that a commercial field-test kit to control water contamination by herbicides performs in compliance with the EU and USA legislations regarding the limit of detection reached. Evaluation and validation studies have been performed using spiked, certified (AquaCheck) and real environmental samples comparing the results with chromatographic methods. In spite of the semiquantitative–qualitative character of the kit, the results demonstrate that the test SensioScreen® TR500 provides a very good estimation of the atrazine concentration in the sample. This test format could be adapted to the detection of other contaminants of environmental relevance.This work has been supported by CICYT (BIO2000-0351-P4-05) and by INIA (VIN0053-C3).Peer reviewe

Palladium–mediated organofluorine chemistry

Producción CientíficaThe substitution of fluorine for hydrogen in a molecule may result in profound changes in its properties and behaviour. Fluorine does not introduce special steric constraints since the F atom has a small size. However, the changes in bond polarity and the possibility of forming hydrogen bonds with other hydrogen donor fragments in the same or other molecules, may change the solubility and physical properties of the fluorinated compound when compared to the non-fluorinated one. Fluorine forms strong bonds to other elements and this ensures a good chemical stability. Altogether, fluorinated compounds are very attractive in materials chemistry and in medicinal chemistry, where many biologically active molecules and pharmaceuticals do contain fluorine in their structure and this has been shown to be essential for their activityJunta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA302U13)Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA256U13

(2R)-2-Benzene­sulfonamido-2-phenyl­ethanoic acid

In the title compound, C14H13NO4S, the dihedral angle between the aromatic ring planes is 45.52 (18)°. In the crystal structure, inter­molecular N—H⋯O and O—H⋯O hydrogen bonds lead to chains of mol­ecules propagating in [100] in which the the ring motifs R 2 1(8), R 2 2(8) and R 3 3(11) are apparent. These polymeric chains are linked through C—H⋯O inter­actions

(2S)-3-Carbamoyl-2-(4-meth­oxy­benzene­sulfonamido)­propanoic acid

In the title compound, C11H14N2O6S, an amino acid-derived sulfonamide, the acetamido group and the carb­oxy­lic group are oriented at dihedral angles of 45.84 (5)° and 47.97 (5)° respectively, with respect to the aromatic ring. In the crystal, the mol­ecules are connected by N—H⋯O and O—H⋯O hydrogen bonds and weak C—H⋯O inter­actions, forming a three-dimensional network

Evaluation of a field-test kit for triazine herbicides (SensioScreen® TR500) as a fast assay to detect pesticide contamination in water samples

11 pages, 3 figures, 7 tables.-- Available online Nov 16, 2002.A field-test kit (SensioScreen® TR500) for the determination of triazine herbicides in water samples has been evaluated. The test is based on an ELISA method performed on a membrane that allows the visual estimation of the presence of triazine herbicides in <10 min. The kit contains all necessary reagents and equipment, and operates on a very simple protocol. The detection level of this semiquantitative kit has been set at 0.5 μg/l (for the sum of all triazines), although concentration levels of 0.1 μg/l for atrazine can be detected. This is the first time that a commercial field-test kit to control water contamination by herbicides performs in compliance with the EU and USA legislations regarding the limit of detection reached. Evaluation and validation studies have been performed using spiked, certified (AquaCheck) and real environmental samples comparing the results with chromatographic methods. In spite of the semiquantitative–qualitative character of the kit, the results demonstrate that the test SensioScreen® TR500 provides a very good estimation of the atrazine concentration in the sample. This test format could be adapted to the detection of other contaminants of environmental relevance.This work has been supported by CICYT (BIO2000-0351-P4-05) and by INIA (VIN0053-C3).Peer reviewe