Aleurocanthus woglumi (Hemiptera: Aleyrodidae) in Citrus: Opportunities and Challenges to Implement a Sustainable Management

Citrus BlackFly (CBF) always represented a threat to Brazil. The impact of the introduction in Brazil of the CBF has led to serious economic and environmental consequences. In this chapter, we will show relevant information on biological aspects, history of occurrence, and impact of CBF on Citrus in Brazil; data about dynamics populations and spatial distribution patterns and dependence will be presented. We are intending to emphasize in this chapter the main challenges and opportunities of some important tactics to promote sustainable management of CBF in citrus, such as: (i) biological control, (ii) chemical and others methods, and (iii) induced resistance

Análise de Perigos e Pontos Críticos de Controle em Processamento de Abate de Bovinos

Em um mundo globalizado onde a competência marca a subsistência das empresas, a busca da qualidade assume um papel preponderante, convertendo-se na ferramenta básica para a competitividade. Os princípios para a melhoria da qualidade aplicam-se tanto a pequenas empresas como a grandes corporações, tanto à s indústrias de serviços como à s de fabricação, e tanto ao setor público como à s empresas privadas. Desta forma, o HACCP surge como elemento essencial (Hazard Analysis Critical Control Point)

Biological characteristics of Trichospilus diatraeae (Hymenoptera: Eulophidae) are influenced by the number of females exposed per pupa of Tenebrio molitor (Coleoptera: Tenebrionidae).

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Ion irradiation of acetylene ice in the ISM and the outer Solar system: laboratory simulations

Acetylene, C2H2, has been observed in the interstellar medium, mostly around young stellar objects, as well as in molecular clouds and cometary comae, representing an important species of astrophysical interest. In this work, we present a laboratory study of the C2H2 radiolysis at 45 K for three different beams and energies: 1.0 MeV H+ and He+, and 1.0 and 1.5 MeV N+ beams. Fourier transform infrared spectroscopy was used for monitoring the molecular changes induced by the ion processing. Two different sample thicknesses were irradiated; for the thicker one, implantation had occurred. Spectra and absorbance evolutions for the thin and thick films are qualitatively different. Four C2H2 bands are observed at 3225, 1954, 1392, and 763 cm-1. The C2H2 compaction and apparent destruction cross-sections are determined. For the case of the H+ beam, the compaction cross-section dominates. Concerning molecular synthesis by irradiation, New product bands were not observed in the thin ice irradiations; for the thicker film ice, the daughter species CH4, C2H4, C3H6, and C4H4 have been identified and their destruction and formation cross-sections determined. The apparent destruction cross-section was found to be a function of the electronic stopping power (Se) as σd ∝ S 3/2e . The half-life of the C2H2 bombarded by galactic cosmic rays is estimated. The current findings are a contribution to the understanding of how the molecules synthesized upon irradiation of Interstellar and outer Solar system ices participate to the molecular enrichment and to the physicochemical evolution of the Universe

HIGH DOSE CARBOPLATIN, ETOPOSIDE, MELPHALAN and AUTOLOGOUS HEMATOPOIETIC STEM CELL RESCUE WITH for the TREATMENT of RELAPSED PEDIATRIC GERM CELL TUMORS

Inst Oncol Pediat, São Paulo, BrazilWeb of Scienc

Combinations of Metarhizium anisopliae with chemical insecticides and their effectiveness in Mahanarva fimbriolata (Hemiptera: Cercopidae) control on sugarcane

Some insecticides can be used jointly with entomopathogenic fungi, and therefore the combi- nation of chemical and biological control measures can be a safe and effective method to con- trol insect pests. The aim of this study was to evaluate the costs and efficacy of combinations of Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) with thiameth- oxam and imidacloprid on spittlebug (Mahanarva fimbriolata (Stål); Hemiptera: Cercopidae) control on sugarcane. The experiment was conducted as a randomized block design (RBD) with 10 treatments and 4 replications. The treatments included a control (untreated), thia- −1 −1 12 −1 methoxam (250 g ha ), imidacloprid (700 g ha ), M. anisopliae (M. a.) (3 × 10 conidia ha ), A1 (3 × 10 12 M. a. conidia ha −1 + 65 g ha −1 of thiamethoxam), A2 (3 × 10 12 M. a. conidia ha −1 + 125 g ha −1 of thiamethoxam), A3 (3 × 10 12 M. a. conidia ha −1 + 187.5 g ha −1 of thiamethoxam), A4 (3 × 10 12 M. a. conidia ha −1 + 175 g ha −1 of imidacloprid), A5 (3 × 10 12 M. a. conidia ha −1 + 350 g ha −1 of imidacloprid), and A6 (3 × 10 12 M. a. conidia ha −1 + 525g ha −1 of imidacloprid). The reductions in the numbers of M. fimbriolata nymphs per treatment compared to the control were similar at 15 DAT (days after treatment) in all treatments except combination A5 (M. anisopliae and thiamethoxam). At 30 DAT, the numbers of nymphs were significantly reduced in all treatments except A3, and their effectiveness ranged from 14.28% to 92.85%. At 45 DAT the numbers of M. fimbriolata nymphs per treatment were significantly reduced in the following treatments: imidacloprid alone at 700g ha -1 , A1, A2, A3, A4 and A6; and the combinations A1 and A2 caused the lowest M. fimbriolata nymph infestations and effectiveness rates of 77.41 and 87.09 %, respectively. At 75 DAT the 2 best control efficacies occurred in treatments A1 (3 × 10 12 M. a. conidia ha -1 of + 65g ha -1 of thiamethoxam) (82.1%) and A5 (78.6%) (3 × 10 12 M. a. conidia ha −1 + 350 g ha −1 of imidacloprid). At 90 DAT the number of nymphs in the control had increased 2.8 fold over the number at 75 DAT. Very good control efficacies at 90 DAT occurred in all treatments with the combination of the fungus with an insecticide. At 105 DAT the numbers of nymphs had surged in all treatments, and no treatment provided effective control. The treatments with the highest earnings per hectare were A1 (3 × 10 12 M. a. conidia ha -1 + 65 g thiamethoxam) and M. anisopliae alone at the recommended dose of 3 × 10 12 M. a. conidia ha -1 . Our findings demonstrate the effectiveness of using either thiamethoxam or imidacloprid in combination with M. anisopliae to control M. fimbriolata nymphs on sugarcane, but greater net earnings per hectare occurred with the lowest rate of the thiamethoxam combination than with any of the imidacloprid combinations.Algunos insecticidas se puede utilizar con hongos entomopatógenos y por lo tanto, la aso- ciación de los controles químico y biológico puede ser una estrategia segura y eficaz para el control de insectos-plaga. El objetivo de este estudio fue evaluar los costos y eficacia de combinaciones de Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavi- cipitaceae) con insecticidas thiamethoxam e imidacloprid para el control de la chicharrita (Mahanarva fimbriolata (Stål); Hemiptera: Cercopidae) en caña de azúcar . El experimento fue conducido en un delineamiento en bloques casualizados (DBC), con 10 tratamientos y 4 repeticiones. Los tratamientos que incluidos el control (sin tratamiento), thiamethoxam (250 g ha −1 ), imidacloprido (700 g ha −1 ), M. anisopliae (M.a.) (3×10 12 conidios ha −1 ), A1 (3×10 12 conidios ha −1 de M. a. + 65 g ha −1 de thiamethoxam), A2 (3×10 12 conidios ha −1 de M. a. + 125g ha −1 de thiamethoxam), A3 (3×10 12 conidios ha −1 de M. a. + 187.5 g ha −1 de thiamethoxam), A4 (3×10 12 conidios ha −1 de M.a + 175 g ha −1 de imidacloprido), A5 (3×10 12 conidios ha −1 de M. a. + 350 g ha −1 de imidacloprido) y A6 (3×10 12 conidios ha −1 de M. a. + 525g ha −1 de imidacloprido). Las reducciones en el número de ninfas M. fimbriolata por tratamiento en comparación con el control fueron similares a los 15 DAT (días pos tratamiento) en todos los tratamientos excepto A5 combinación (M. anisopliae y thiamethoxam). A los 30 DAT, el número de ninfas se redujeron significativamente en todos los tratamientos, excepto A3, y su eficacia varió de 14,28% para 92,85%. A los 45 DAT, los números de ninfas M. fimbriolata por tratamiento se redujeron significativamente en los siguientes tratamientos: imidacloprido solo en 700 g ha -1 , A1, A2, A3, A4 y A6; y las combinaciones de A1 y A2 causaron la más bajo infestaciones de ninfas M. fimbriolata y sus tasas de eficacia fueron de 77,41 y 87,09%, respectivamente. A los 75 DAT, los 2 mejores eficacias de control se produjeron en tratamientos A1 (3×10 12 conidios ha −1 de M. a. + 65 g ha −1 de thiamethoxam) y A5 (78.6%) (3×10 12 conidios ha −1 de M. a.+ 350 g ha −1 de imidacloprido). A los 90 DAT, el número de ninfas en el control había aumentado 2,8 veces más el número a 75 DAT. Muy buenas eficacias de control en 90 DAT, se produjo en todos los tratamientos con la combinación del hongo con un insecticida. A los 105 DAT, el número de ninfas habían aumentado en todos los tratamientos, y ningún tratamiento había proporcionado un control efectivo. Los tratamientos con los mayores rendimientos hectárea fueron A1 (3×10 12 conidios ha −1 de M. a.+ 65 g de thiamethoxam) y M. anisopliae solo a la dosis recomendada de 3×10 12 conidios ha −1 de M. a. Nuestros resultados demuestran la eficacia de thiamethoxam y imidacloprido en combinación con M. anisopliae para el control de ninfas M. fimbriolata en caña de azúcar, pero mayores beneficio neto por hectárea se produjeron con la tasa más baja de la combinación de thiamethoxam que con cualquiera de las combinaciones de imidacloprid

Composição química e estudo da atividade antibacteriana de Bowdichia virgilioides Kunth (Sucupira) – Fabaceae – Papilonoidae

Bowdichia virgilioides (Fabaceae - Papilionoidea), popularmente conhecida como Sucupira-preta, é uma espécie arbórea nativa do Brasil utilizadas na medicina popular para infecções de garganta. Com base nessas evidências, e com o interesse para justificar o uso popular, este trabalho teve como objetivo verificar a atividade antibacteriana in vitro de extratos brutos e fracionados de cascas e cerne da planta. Observou-se pela conclusão do levantamento fitoquímico a presença de taninos e flavonóides nas cascas e no cerne, e alcalóides apenas encontrados na casca. A análise por HPLC revelou a presença de flavonóides e ácidos fenólicos, produtos naturais, com diversas atividades biológicas, incluindo a atividade modificadora antibiótica. Todos os microorganismos foram inibidos apenas com o CIM ≥ 1024 μg/mL. No entanto, quando associado a antibióticos aminoglicosídeos, foi demonstrada potenciação destes em quase todos os produtos testados e em pelo menos uma bactéria foi observada uma atividade moduladora significativa

Characterization of MTAP gene expression in breast cancer patients and cell lines

MTAP is a ubiquitously expressed gene important for adenine and methionine salvage. The gene is located at 9p21, a chromosome region often deleted in breast carcinomas, similar to CDKN2A, a recognized tumor suppressor gene. Several research groups have shown that MTAP acts as a tumor suppressor, and some therapeutic approaches were proposed based on a tumors\ub4 MTAP status. We analyzed MTAP and CDKN2A gene (RT-qPCR) and protein (western-blotting) expression in seven breast cancer cell lines and evaluated their promoter methylation patterns to better characterize the contribution of these genes to breast cancer. Cytotoxicity assays with inhibitors of de novo adenine synthesis (5-FU, AZA and MTX) after MTAP gene knockdown showed an increased sensitivity, mainly to 5-FU. MTAP expression was also evaluated in two groups of samples from breast cancer patients, fresh tumors and paired normal breast tissue, and from formalin-fixed paraffin embedded (FFPE) core breast cancer samples diagnosed as Luminal-A tumors and triple negative breast tumors (TNBC). The difference of MTAP expression between fresh tumors and normal tissues was not statistically significant. However, MTAP expression was significantly higher in Luminal-A breast tumors than in TNBC, suggesting the lack of expression in more aggressive breast tumors and the possibility of using the new approaches based on MTAP status in TNB

Anisotropy and chemical composition of ultra-high energy cosmic rays using arrival directions measured by the Pierre Auger Observatory

The Pierre Auger Collaboration has reported evidence for anisotropy in the distribution of arrival directions of the cosmic rays with energies $E>E_{th}=5.5\times 10^{19}$ eV. These show a correlation with the distribution of nearby extragalactic objects, including an apparent excess around the direction of Centaurus A. If the particles responsible for these excesses at $E>E_{th}$ are heavy nuclei with charge $Z$, the proton component of the sources should lead to excesses in the same regions at energies $E/Z$. We here report the lack of anisotropies in these directions at energies above $E_{th}/Z$ (for illustrative values of $Z=6,\ 13,\ 26$). If the anisotropies above $E_{th}$ are due to nuclei with charge $Z$, and under reasonable assumptions about the acceleration process, these observations imply stringent constraints on the allowed proton fraction at the lower energies