DNA Methylation in Rectal Cancer

Rectal cancer differs from colon cancer in terms of its underlying biological behaviour, clinical course, genetic and epigenetic aetiology. Epigenetic mechanisms modify gene expression independently of DNA sequence. DNA methylation is the most studied epigenetic mechanism, known to play a role in colorectal cancer. The role of DNA methylation specific to rectal cancer however, is poorly understood. Here we present the results from a DNA methylation study of 45 individuals with rectal cancer. A total of 45 patients (>18 years) diagnosed with rectal adenocarcinoma (stages II and III) who underwent or were undergoing treatment were recruited. Matched rectal tumour and adjacent normal mucosal samples (n=90) were obtained from each patient and processed fresh frozen (n=32) or embedded in paraffin (n=58). DNA was extracted and checked for quality and quantity, treated with sodium bisulfite and run on the Illumina Infinium HumanMethylation 450 Beadchip. Only samples that passed the Quality Control were subsequently analysed (n=30). A combined linear regression analysis of all 408,652 probes showed that global levels of DNA methylation are decreased in rectal cancer samples compared with normal unaffected samples. In total, 176 differentially methylated probes and 828 differentially methylated regions were identified in rectal cancer vs normal tissue. All the genes identified underwent gene ontology analysis to assess whether they are biologically meaningful. In summary, our study focused on the discovery of de novo epigenetic changes associated with rectal cancer, using a genome wide approach and novel bioinformatics approaches. These findings improve our understanding of the epigenetics of this disease. Furthermore, they have the potential to be used as biomarkers for detection, prognosis and monitoring treatment response in patients with rectal cancer.The Jean Shanks FoundationBowel Cancer WestThe Royal College of Surgeon

Impact of Emerging Interaction Techniques on Energy Use in the UK Social Housing

End use energy efficiency and fuel poverty is one of the major issues in the UK social housing sector. It is estimated that about 10% of English households live in fuel poverty. During 2015 UK greenhouse gas emission final figures show that the net CO2 emission was reduced by 4.1% between 2014 and 2015. This shows that the UK is on course to attain its second carbon budget with annual 2013–2015 emissions that are each below the estimated level for the period. However, the housing sector lags with a 4% increase in emissions over the same period. More work needs to be done in this sector. Householders can adopt more efficient energy use approaches and make better lifestyle choices to save money and have a safer environment. This research addresses government priorities to reduce energy demand, meet CO2 reduction targets, and reduce domestic reliance on fossil fuels, offering protection from price risks and fuel poverty as well as providing more affordable and comfortable domestic environments. The proposed research paper deals with novel interaction methods on energy use in social housing and how the aforesaid issues can be reflected on. A detailed background study on existing interaction methods and ongoing development of a serious game trialled in 19 households has been carried out. It has been noted that displaying real-time utility use and indoor environmental conditions to householders increased awareness and impacted how energy is being consumed. Furthermore, the proposed paper will investigate end use energy profile pattern changes due to novel interaction methods

Development and evaluation of a method to estimate the potential of decarbonisation technologies deployment at higher education campuses

peer-reviewedThis paper describes a method for the quantitative estimation of the potential of decarbonisation technologies deployed on Higher Education Campuses (HEC). This was developed to fill the need for a practicable and standardised method to provide preliminary estimations of the deployment potential of building integrated photovoltaics (PV), micro-wind turbines, rainwater harvesting and ground mounted PV at HECs. The method identifies two key variables, namely roof area and open carpark area, to aid estimation of decarbonisation technologies deployment at HECs, using Google Earth imagery coupled with publicly available online HEC maps. The method was trialled for the higher education sector in Ireland identifying major potential for deployment of decarbonisation technologies for the sector. The building decarbonisation aspect of the proposed approach is applicable to sectors outside HEC particularly commercial and industrial sectors due to similarity in building footprint characteristics. The open carpark component of the methodology is also applicable to city-scale analysis due to uniformity in form of open carparks worldwide. This highlights the usefulness of this method through informing city-scale transitions towards decarbonisatio

Data integration for offshore decommissioning waste management

Offshore decommissioning represents significant business opportunities for oil and gas service companies. However, for owners of offshore assets and regulators, it is a liability because of the associated costs. One way of mitigating decommissioning costs is through the sales and reuse of decommissioned items. To achieve this effectively, reliability assessment of decommissioned items is required. Such an assessment relies on data collected on the various items over the lifecycle of an engineering asset. Considering that offshore platforms have a design life of about 25 years and data management techniques and tools are constantly evolving, data captured about items to be decommissioned will be in varying forms. In addition, considering the many stakeholders involved with a facility over its lifecycle, information representation of the items will have variations. These challenges make data integration difficult. As a result, this research developed a data integration framework that makes use of Semantic Web technologies and ISO 15926 - a standard for process plant data integration - for rapid assessment of decommissioned items. The proposed solution helps in determining the reuse potential of decommissioned items, which can save on cost and benefit the environment

Focusing the management of rectal cancer.

Rectal cancer treatment has undergone major changes over the last 15 years with a focus on individualized care based around MRI assessment of the relationship of the tumour to the mesorectal fascia, improved surgical techniques and targeted use of pre-operative oncological therapies in patients with locally advanced disease. The recognition that some tumours responded completely to pre-operative chemoradiotherapy, and the selective use of a non-operative policy has led to a quest to further identify those patients and their tumour in whom this approach could be used, irrespective of MRI stage. With no clear patient factors identified, the tumour and its gene expression has become a target for research to identify individual single-nucleotide polymorphisms, which may indicate a response to specific treatment, or not. To date some agents have been identified and trialed, such as cetuximab, with individual tumours being assessed for response allowing directed treatment. The reviewed paper by Sebio and colleagues report a study that links polymorphisms in the DNA repair gene XRCC1 with response to neoadjuvant 5-Fluorouracil treatment in rectal cancer patients. However, genetic heterogeneity alone may not explain the variations of drug response and environmental factors may lead to epigenetic effects and therefore alter responses. Therefore whilst this study demonstrates the impact of different single nucleotide polymorphisms (SNPs), it is only one step forward, but perhaps a step in the right direction.This article is freely available from PubMed Central - click on the Additional Link above