Downregulation Of 14q32 Micrornas In Primary Human Desmoplastic Medulloblastoma

Medulloblastoma (MB) is one of the most common pediatric cancers, likely originating from abnormal development of cerebellar progenitor neurons. MicroRNA (miRNA) has been shown to play an important role in the development of the central nervous system. Microarray analysis was used to investigate miRNA expression in desmoplastic MB from patients diagnosed at a young age (1 or 2 years old). Normal fetal or newborn cerebellum was used as control. A total of 84 differentially expressed miRNAs (64 downregulated and 20 upregulated) were found. Most downregulated miRNAs (32/64) were found to belong to the cluster of miRNAs at the 14q32 locus, suggesting that this miRNA locus is regulated as a module in MB. Possible mechanisms of 14q32 miRNAs downregulation were investigated by the analysis of publicly available gene expression data sets. First, expression of estrogen-related receptor-γ (ESRRG), a reported positive transcriptional regulator of some 14q32 miRNAs, was found downregulated in desmoplastic MB. Second, expression of the parentally imprinted gene MEG3 was lower in MB in comparison to normal cerebellum, suggesting a possible epigenetic silencing of the 14q32 locus. miR-129-5p (11p11.2/7q32.1), miR-206 (6p12.2), and miR-323-3p (14q32.2), were chosen for functional studies in DAOY cells. Overexpression of miR-129-5p using mimics decreased DAOY proliferation. No effect was found with miR-206 or miR-323 mimics. © 2013 Lucon, Rocha, Craveiro, Dilloo, Cardinalli, Cavalcanti, Aguiar, Maurer-Morelli and Yunes.3 SEPEllison, D., Classifying the medulloblastoma: insights from morphology and molecular genetics (2002) Neuropathol Appl Neurobiol, 28, pp. 257-282. , doi:10.1046/j.1365-2990.2002.00419.xGulino, A., Di Marcotullio, L., Ferretti, E., De Smaele, E., Screpanti, I., Hedgehog signaling pathway in neural development and disease (2007) Psychoneuroendocrinology, 32, pp. S25-56. , doi:10.1016/j.psyneuen.2007.03.017Kool, M., Koster, J., Bunt, J., Hasselt, N.E., Lakeman, A., van Sluis, P., Integrated genomics identifies five medulloblastoma subtypes with distinct genetic profiles, pathway signatures and clinicopathological features (2008) PLoS ONE, 3, pp. e3088. , doi:10.1371/journal.pone.0003088Northcott, P.A., Korshunov, A., Witt, H., Hielscher, T., Eberhart, C.G., Mack, S., Medulloblastoma comprises four distinct molecular variants (2010) J Clin Oncol, 29, pp. 1408-1414. , doi:10.1200/JCO.2009.27.4324Gilbertson, R.J., Ellison, D.W., The origins of meduloblastoma subtypes (2008) Annu Rev Pathol, 3, pp. 341-365. , doi:10.1146/annurev.pathmechdis.3.121806.151518Schüller, U., Heine, V.M., Mao, J., Kho, A.T., Dillon, A.K., Han, Y.G., Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form SHH-induced medulloblastoma (2008) Cancer Cell, 14, pp. 123-134. , doi:10.1016/j.ccr.2008.07.005Yang, Z.J., Ellis, T., Markant, S.L., Read, T.A., Kessler, J.D., Bourboulas, M., Medulloblastoma can be initiated by deletion of Patched in lineage-restricted progenitors or stem cells (2008) Cancer Cell, 14 (2), pp. 135-145. , doi:10.1016/j.ccr.2008.07.003Gibson, P., Tong, Y., Robinson, G., Thomson, M.C., Currie, D.S., Eden, C., Subtypes of medulloblastoma have distinct developmental origins (2010) Nature, 468, pp. 1095-1099. , doi:10.1038/nature09587Cohen, S.M., (2010) MiRNAs in CNS Development and Neurodegeneration: Insights from Drosophila Genetics, , http://www.springer.com/978-3-642-04297-3, Springer Available fromSood, P., Krek, A., Zavolan, M., Macino, G., Rajewsky, N., Cell-type-specific signatures of microRNAs on target mRNA expression (2006) Proc Natl Acad Sci U S A, 103 (8), pp. 2746-2751. , doi:10.1073/pnas.0511045103Guo, H., Ingolia, N.T., Weissman, J.S., Bartel, D.P., Mammalian microRNAs predominantly act to decrease target mRNA levels (2010) Nature, 466 (7308), pp. 835-841. , doi:10.1038/nature09267Ferretti, E., De Smaele, E., Po, A., Di Marcotullio, L., Tosi, E., Espínola, M.S.B., MiRNA profiling in human meduloblastoma (2009) Int J Cancer, 124, pp. 568-577. , doi:10.1002/ijc.23948Northcott, P.A., Fernandez, L.A., Hagan, J.P., Ellison, D.W., Grajkowska, W., Gillespie, Y., The miR-17/92 polycistron is upregulated in Sonic Hedgehog driven medulloblastomas and induced by N-myc in Sonic Hedgehog-treated cerebellar neural precursors (2009) Cancer Res, 69, pp. 3249-3255. , doi:10.1158/0008-5472.CAN-08-4710Cho, Y.J., Tsherniak, A., Tamayo, P., Santagata, S., Ligon, A., Greulich, H., Integrative genomic analysis of medulloblastoma identifies a molecular subgroup that drives poor clinical outcome (2011) J Clin Oncol, 29 (11), pp. 1424-1430. , doi:10 1200/JCO. 2010. 28. 5148Fernandez, L.A., Northcott, P.A., Taylor, M.D., Kenney, A.M., Normal and oncogenic roles for microRNAs in the developing brain (2009) Cell Cycle, 8 (24), pp. 4049-4054. , doi:10.4161/cc.8.24.10243Pfister, S.M., Korshunov, A., Kool, M., Hasselblatt, M., Eberhart, C., Taylor, M.D., Molecular diagnostics of CNS embryonal tumors (2010) Acta Neuropathol, 120, pp. 553-566. , doi:10.1007/s00401-010-0751-5Livak, K.J., Schmittgen, T.D., Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method (2001) Methods, 25, pp. 402-408. , doi:10.1006/meth.2001.1262Pietsch, T., Scharmann, T., Fonatsch, C., Schmidt, D., Ockler, R., Freihoff, D., Characterization of five new cell lines derived from human primitive neuroectodermal tumors of the central nervous system (1994) Cancer Res, 54, pp. 3278-3287Breitling, R., Armengaud, P., Amtmann, A., Herzyk, P., Rank products: a simple, yet powerful, new method to detect differentially regulated genes in replicated microarray experiments (2004) FEBS Lett, 573, pp. 83-92. , doi:10.1016/j.febslet.2004.07.055Gautier, L., Cope, L., Bolstad, B.M., Irizarry, R.A., Affy - analysis of Affymetrix GeneChip data at the probe level (2004) Bioinformatics, 20, pp. 307-315. , doi:10.1093/bioinformatics/btg405Gentleman, R.C., Carey, V.J., Bates, D.M., Bolstad, B., Dettling, M., Dudoit, S., Bioconductor: open software development for computational biology and bioinformatics (2004) Genome Biol, 5, pp. R80Zhou, J., Tian, Y., Li, J., Lu, B., Sun, M., Zou, Y., miR-206 is down-regulated in breast cancer and inhibits cell proliferation through the up-regulation of cyclinD2 (2013) Biochem Biophys Res Commun, 433 (2), pp. 207-212. , doi:10.1016/j.bbrc.2013.02.084Birks, D.K., Barton, V.N., Donson, A.M., Handler, M.H., Vibhakar, R., Foreman, N.K., Survey of MicroRNA expression in pediatric brain tumors (2011) Pediatr Blood Cancer, 56 (2), pp. 211-216. , doi:10.1002/pbc.22723Huang, Y.W., Liu, J.C., Deatherage, D.E., Luo, J., Mutch, D.G., Goodfellow, P.J., Epigenetic repression of microRNA-129-2 leads to upregulation of SOX4 oncogene in endometrial cancer (2009) Cancer Res, 69 (23), pp. 9038-9046. , doi:10.1158/0008-5472.CAN-09-1499Shen, R., Pan, S., Qi, S., Lin, X., Cheng, S., Epigenetic repression of microRNA-129-2 leads to upregulation of SOX4 in gastric cancer (2010) Biochem Biophys Res Commun, 394 (4), pp. 1047-1052. , doi:10.1016/j.bbrc.2010.03.121Neben, K., Korshunov, A., Benner, A., Wrobel, G., Hahn, M., Kokocinski, F., Microarray-based screening for molecular markers in medulloblastoma revealed STK15 as independent predictor for survival (2004) Cancer Res, 64 (9), pp. 3103-3111. , doi:10.1158/0008-5472.CAN-03-3968de Bont, J.M., Kros, J.M., Passier, M.M., Reddingius, R.E., Sillevis Smitt, P.A., Luider, T.M., Differential expression and prognostic significance of SOX genes in pediatric medulloblastoma and ependymoma identified by microarray analysis (2008) Neuro Oncol, 10 (5), pp. 648-660. , doi:10.1215/15228517-2008-032Garzia, L., Andolfo, I., Cusanelli, E., Marino, N., Petrosino, G., De Martino, D., MicroRNA-199b-5p impairs cancer stem cells through negative regulation of HES1 in medulloblastoma (2009) PLoS ONE, 4, p. 4998. , doi:10.1371/journal.pone.0004998Gokhale, A., Kunder, R., Goel, A., Sarin, R., Moiyadi, A., Shenoy, A., Distinctive microRNA signature of medulloblastomas associated with the WNT signaling pathway (2010) J Cancer Res Ther, 6, pp. 521-529. , doi:10.4103/0973-1482.77072de Antonellis, P., Medaglia, C., Cusanelli, E., Andolfo, I., Liguori, L., De Vita, G., MiR-34a targeting of notch ligand delta-like 1 impairs CD15+/CD133+ tumor-propagating cells and supports neural differentiation in medulloblastoma (2011) PLoS ONE, 6 (9), pp. e24584. , doi:10.1371/journal.pone.0024584Grunder, E., D'Ambrosio, R., Fiaschetti, G., Abela, L., Arcaro, A., Zuzak, T., MicroRNA-21 suppression impedes medulloblastoma cell migration (2011) Eur J Cancer, 47, pp. 2479-2490. , doi:10.1016/j.ejca.2011.06.041Uziel, T., Karginov, F.V., Xie, S., Parker, J.S., Wang, Y.D., Gajjar, A., The miR-17 92 cluster collaborates with the Sonic Hedgehog pathway in medulloblastoma (2009) Proc Natl Acad Sci USA, 106, pp. 2812-2817. , doi:10.1073/pnas.0809579106Chédotal, A., Kerjan, G., Moreau-Fauvarque, C., The brain within the tumor: new roles for axon guidance molecules in cancers (2005) Cell Death Differ, 12 (8), pp. 1044-1056. , doi:10.1038/sj.cdd.4401707Aref, D., Moffatt, C.J., Agnihotri, S., Ramaswamy, V., Dubuc, A.M., Northcott, P.A., Canonical TGF-b pathway activity is a predictor of SHH-driven medulloblastoma survival and delineates putative precursors in cerebellar development (2013) Brain Pathol, 23, pp. 178-191. , doi:10.1111/j.1750-3639.2012.00631.xLuo, X., Liu, J., Cheng, S.Y., The role of microRNAs during the genesis of medulloblastoma induced by the hedgehog pathway (2011) Biomed Res, 25 (1), pp. 42-48. , doi:10. 1016/S1674-8301(11)Northcott, P.A., Shih, D.J., Peacock, J., Garzia, L., Morrissy, A.S., Zichner, T., Subgroup-specific structural variation across 1,000 medulloblastoma genomes (2012) Nature, 488 (7409), pp. 49-56. , doi:10.1038/nature11327Song, G., Wang, L., miR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus (2008) PLoS ONE, 3, pp. e3574. , doi:10.1371/journal.pone.0003574Remke, M., Hielscher, T., Korshunov, A., Northcott, P.A., Bender, S., Kool, M., FSTL5 is a marker of poor prognosis in non-WNT/non-SHH medulloblastoma (2011) J Clin Oncol, 29 (29), pp. 3852-3861. , doi:10.1200/JCO.2011.36.2798Edgar, R., Domrachev, M., Lash, A.E., Gene expression omnibus: NCBI gene expression and hybridization array data repository (2002) Nucleic Acids Res, 30, pp. 207-210. , doi:10.1093/nar/30.1.207Yu, S., Wang, X., Ng, C.F., Chen, S., Chan, F.L., ERRgamma suppresses cell proliferation and tumor growth of androgen-sensitive and androgen-insensitive prostate cancer cells and its implication as a therapeutic target for prostate cancer (2007) Cancer Res, 67 (10), pp. 4904-4914. , doi:10.1158/0008-5472.CAN-06-3855Mancuso, M., Leonardi, S., Giardullo, P., Pasquali, E., Borra, F., Stefano, I.D., The estrogen receptor beta agonist diarylpropionitrile (DPN) inhibits medulloblastoma development via anti-proliferative and pro-apototic pathways (2011) Cancer Lett, 308 (2), pp. 197-202. , doi:10.1016/j.canlet.2011.05.004da Rocha, S.T., Edwards, C.A., Ito, M., Ogata, T., Ferguson-Smith, A.C., Genomic imprinting at the mammalian Dlk1-Dio3 domain (2008) Trends Genet, 24, pp. 306-316. , doi:10.1016/j.tig.2008.03.011Lin, S.P., Youngson, N., Takada, S., Seitz, H., Reik, W., Paulsen, M., Asymmetric regulation of imprinting on the maternal and paternal chromosomes at the Dlk1-Gtl2 imprinted cluster on mouse chromosome 12 (2003) Nat Genet, 35, pp. 97-102. , doi:10.1038/ng1233Takada, S., Paulsen, M., Tevendale, M., Tsai, C.E., Kelsey, G., Cattanach, B.M., Epigenetic analysis of the Dlk1-Gtl2 imprinted domain on mouse chromosome implications for imprinting control from comparison with Igf2-H19 (2002) Hum Mol Genet, 12, pp. 77-86. , doi:10.1093/hmg/11.1.77Kagami, M., O'Sullivan, M.J., Green, A.J., Watabe, Y., Arisaka, O., Masawa, N., The IG-DMR and the MEG3-DMR at human chromosome 14q32 2: hierarchical interaction and distinct functional properties as imprinting control centers (2010) PLoS Genet, 6, pp. e1000992. , doi:10.1371/journal.pgen.1000992Diede, S.J., Guenthoer, J., Geng, L.N., Mahoney, S.E., Marotta, M., Olson, J.M., DNA methylation of developmental genes in pediatric medulloblastomas identified by denaturation analysis of methylation differences (2010) Proc Natl Acad Sci U S A, 107 (1), pp. 234-239. , doi:10.1073/pnas.0907606106Thayanithy, V., Park, C., Sarver, A.L., Kartha, R.V., Korpela, D.M., Graef, A.J., Combinatorial treatment of DNA and chromatin-modifying drugs cause cell death in human and canine osteosarcoma cell lines (2012) PLoS ONE, 7 (9), pp. e43720. , doi:10.1371/journal.pone.0043720Pierson, J., Hostager, B., Fan, R., Vibhakar, R., Regulation of cyclin dependent kinase 6 by microRNA 124 in medulloblastoma (2008) J Neurooncol, 90, pp. 1-7. , doi:10.1007/s11060-008-9624-3Li, K.K., Pang, J.C., Ching, A.K., Wong, C.K., Kong, X., Wang, Y., miR-124 is frequently down-regulated in meduloblastoma and is a negative regulator of SLC16A1 (2009) Hum Pathol, 40 (9), pp. 1234-1243. , doi:10.1016/j.humpath.2009.02.003Ferretti, E., De Smaele, E., Miele, E., Laneve, P., Pó, A., Pelloni, M., Concerted miRNA control of Hedgehog signalling in cerebellar neuronal progenitor and tumour cells (2008) EMBO J, 27, pp. 2616-2627. , doi:10.1038/emboj.2008.172Venkataraman, S., Alimova, I., Fan, R., Harris, P., Foreman, N., Vibhakar, R., MicroRNA 128a increases intracellular ROS level by targeting Bmi-1 and inhibits medulloblastoma cancer cell growth by promoting senescence (2010) PLoS ONE, 5 (6), pp. e10748. , doi:10.1371/journal.pone.0010748Genovesi, L.A., Carter, K.W., Gottardo, N.G., Giles, K.M., Dallas, P.B., Integrated analysis of miRNA and mRNA expression in childhood medulloblastoma compared with neural stem cells (2011) PLoS ONE, 6 (9), pp. e23935. , doi:10.1371/journal.pone.0023935Weeraratne, S.D., Amani, V., Neiss, A., Teider, N., Scott, D.K., Pomeroy, S.L., miR-34a confers chemosensitivity through modulation of MAGE-A and p53 in medulloblastoma (2011) Neuro Oncol, 13 (2), pp. 165-175. , doi:10.1093/neuonc/noq179Lv, S.Q., Kim, Y.H., Giulio, F., Shalaby, T., Nobusawa, S., Yang, H., Genetic alterations in MicroRNAs in medulloblastomas (2012) Brain Pathol, 22, pp. 230-239. , doi:10.1111/j.1750-3639.2011.00523.xWang, X.M., Zhang, S.F., Cheng, Z.Q., Peng, Q.Z., Hu, J.T., Gao, L.K., MicroRNA383 regulates expression of PRDX3 in human medulloblastomas (2012) Zhonghua Bing Li Xue Za Zhi, 41 (8), pp. 547-552. , doi:10.3760/cma.j.issn.0529-5807.2012.08.009Li, K.K.W., Pang, J.C.S., Lau, K.M., Zhou, L., Mao, Y., Wang, Y., MiR-383 is downregulated in medulloblastoma and targets peroxiredoxin 3 (PRDX3) (2013) Brain Pathol, 23 (4), pp. 413-425. , doi:10.1111/bpa.12014Weeraratne, S.D., Amani, V., Teider, N., Pierre-Francois, J., Winter, D., Kye, M.J., Pleiotropic effects of miR-183 96 182 converge to regulate cell survival, proliferation and migration in meduloblastoma (2012) Acta Neuropathol, 123, pp. 539-552. , doi:10.1007/s00401-012-0969-5Venkataraman, S., Birks, D.K., Balakrishnan, I., Alimova, I., Harris, P.S., Patel, P.R., MicroRNA 218 acts as a tumor suppressor by targeting multiple cancer phenotype-associated genes in medulloblastoma (2013) J Biol Chem, 228 (3), pp. 1918-1928. , doi:10.1074/jbc.M112.396762Wei, L., Yan-hua, G., Teng-fei, C., Xiao-zhong, P., Jian-gang, Y., Zhen-yu, M., Identification of differentially expressed microRNAs by microarray: a possible role for microRNAs gene in medulloblastomas (2009) Chin Med J, 122 (20), pp. 2405-241

Soil cover plants on water erosion control in the South of Minas Gerais

Water erosion is responsible for soil, water, carbon and nutrient losses, turning into the most important type of degradation of Brazilian soils. This study aimed to evaluate the influence of three cover plants under two tillage systems on water erosion control in an Argisol at south of Minas Gerais state, Brazil. The cover plants utilized in the study were pigeon pea, jack bean and millet, under contour seeding and downslope tillage. Experimental plots of 4 x 12 m, with 9% slope, under natural rainfall were used for the quantification of losses of soil, water, nutrients, and organic matter. One experimental plot was kept without plant cover (reference). Higher erosivity was observed in December and January, although a great quantity of erosive rainfall was detected during the whole raining period. Contour seeding provided a greater reduction of water erosion than downslope tillage, as expected. The jack bean under contour seeding revealed the lowest values of soil, water, nutrients and organic matter losses

Measurements of long-range near-side angular correlations in sNN=5\sqrt{s_{\text{NN}}}=5TeV proton-lead collisions in the forward region

Two-particle angular correlations are studied in proton-lead collisions at a nucleon-nucleon centre-of-mass energy of $\sqrt{s_{\text{NN}}}=5$TeV, collected with the LHCb detector at the LHC. The analysis is based on data recorded in two beam configurations, in which either the direction of the proton or that of the lead ion is analysed. The correlations are measured in the laboratory system as a function of relative pseudorapidity, $\Delta\eta$, and relative azimuthal angle, $\Delta\phi$, for events in different classes of event activity and for different bins of particle transverse momentum. In high-activity events a long-range correlation on the near side, $\Delta\phi \approx 0$, is observed in the pseudorapidity range $2.0<\eta<4.9$. This measurement of long-range correlations on the near side in proton-lead collisions extends previous observations into the forward region up to $\eta=4.9$. The correlation increases with growing event activity and is found to be more pronounced in the direction of the lead beam. However, the correlation in the direction of the lead and proton beams are found to be compatible when comparing events with similar absolute activity in the direction analysed.Comment: All figures and tables, along with any supplementary material and additional information, are available at https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-040.htm

Evidence for the strangeness-changing weak decay Ξb−→Λb0π−\Xi_b^-\to\Lambda_b^0\pi^-

Using a $pp$ collision data sample corresponding to an integrated luminosity of 3.0~fb$^{-1}$, collected by the LHCb detector, we present the first search for the strangeness-changing weak decay $\Xi_b^-\to\Lambda_b^0\pi^-$. No $b$ hadron decay of this type has been seen before. A signal for this decay, corresponding to a significance of 3.2 standard deviations, is reported. The relative rate is measured to be ${{f_{\Xi_b^-}}\over{f_{\Lambda_b^0}}}{\cal{B}}(\Xi_b^-\to\Lambda_b^0\pi^-) = (5.7\pm1.8^{+0.8}_{-0.9})\times10^{-4}$, where $f_{\Xi_b^-}$ and $f_{\Lambda_b^0}$ are the $b\to\Xi_b^-$ and $b\to\Lambda_b^0$ fragmentation fractions, and ${\cal{B}}(\Xi_b^-\to\Lambda_b^0\pi^-)$ is the branching fraction. Assuming $f_{\Xi_b^-}/f_{\Lambda_b^0}$ is bounded between 0.1 and 0.3, the branching fraction ${\cal{B}}(\Xi_b^-\to\Lambda_b^0\pi^-)$ would lie in the range from $(0.57\pm0.21)\%$ to $(0.19\pm0.07)\%$.Comment: 7 pages, 2 figures, All figures and tables, along with any supplementary material and additional information, are available at https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-047.htm

BB flavour tagging using charm decays at the LHCb experiment

An algorithm is described for tagging the flavour content at production of neutral $B$ mesons in the LHCb experiment. The algorithm exploits the correlation of the flavour of a $B$ meson with the charge of a reconstructed secondary charm hadron from the decay of the other $b$ hadron produced in the proton-proton collision. Charm hadron candidates are identified in a number of fully or partially reconstructed Cabibbo-favoured decay modes. The algorithm is calibrated on the self-tagged decay modes $B^+ \to J/\psi \, K^+$ and $B^0 \to J/\psi \, K^{*0}$ using $3.0\mathrm{\,fb}^{-1}$ of data collected by the LHCb experiment at $pp$ centre-of-mass energies of $7\mathrm{\,TeV}$ and $8\mathrm{\,TeV}$. Its tagging power on these samples of $B \to J/\psi \, X$ decays is $(0.30 \pm 0.01 \pm 0.01) \%$.Comment: All figures and tables, along with any supplementary material and additional information, are available at http://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2015-027.htm

Vegetative development, fruits yield and optimization of pineapple cv. Pérola with different levels of irrigation

Apesar de ser uma planta com necessidades hídricas relativamente baixas, o abacaxizeiro tem demanda permanente de água, variável ao longo do ciclo e dependente do seu estádio de desenvolvimento. Assim, objetivou-se analisar volumes de irrigação no desenvolvimento vegetativo, no rendimento da fruta e na otimização do abacaxizeiro cv. Pérola. O experimento foi realizado na Universidade Federal de Sergipe, município de São Cristóvão (11°01'S, 37°12'W), no delineamento em blocos ao acaso, com quatro tratamentos (lâminas de água) (100% da evaporação do tanque Classe A (523,7mm ano-1); 75% da evaporação do tanque Classe A (392,8mm ano-1), 50% da evaporação do tanque Classe A (261,8mm ano-1) e 0% da evaporação do tanque Classe A, seis repetições e 12 plantas úteis por parcela. O sistema de irrigação foi por aspersão convencional disposto em linha, com pressão de 20mca e vazão de 1,33m3 h-1. A área foliar (cm2) mínima atingida de 4552,6cm2 foi observada no volume de água de 122,9mm ano-1, enquanto a massa seca das folhas (147,6g) foi constatada com 17mm ano-1. O máximo comprimento da folha D (88,9cm) foi estimado com 532,7 mm ano-1. Já o máximo comprimento do fruto (23cm) foi observado na lâmina de 296,9mm ano-1. A massa do fruto máxima estimado de 1.736g foi constatado na lâmina de 356,4mm ano-1. No contexto, a irrigação contribui de forma positiva no desenvolvimento vegetativo e rendimento da fruta do abacaxizeiro. Ressalta-se remuneração mensal líquida de R 1.161,17ha-1, quando se adota lâmina de irrigação de 356,4mm ano-1. _________________________________________________________________________________________ ABSTRACT: Regardless a relatively low water needs, pineapple fruit has a variable behavior related to water need throughout its biological cycle depending on the development stage. The objective of this work was to analyze irrigation on the vegetative development and fruits yield in pineapple cv. Pérola. The experiment was conducted at the Universidade Federal de Sergipe Experimental Station, at São Cristóvão - SE (11°01'S, 37°12'W), in a randomized block design, with four water level treatments as follow: 100% of Class-A (523.7mm ano-1) evaporation pan; 75% of Class-A (392.8mm ano-1) evaporation pan, 50% of Class-A (261.8mm ano-1) evaporation pan and 0% of Class-A evaporation pan, in six replications and twelve plants per plot. The splinkler watering system of irrigation was installed in a line, with an operating pression of 20 mca and a sprinkler dischrage of 1.33m3h-1. A minimum leaf area of 4552.6cm2 was observed, considering a water level of 122.9mm year-1, while a leaf dry matter was 147.6 g in 17mmyear-1. The maximum leaf length (D) of 88.9cm was estimated considering 532.7mmyear-1. The maximum fruit length of 23cm was observed, considering a water level of 296.9mmyear-1. The maximum estimated fruit weight was 1.736g in a water level of 356.4mm year-1. The irrigation seems to contribute in a positive way to the vegetative development in the pineapple fruit yield. It is important to mention that it could be observed a net profit of R 1,161.17 ha-1, when a water level of 356.4mm ano-1 of irrigation was adopted

Frequency, diversity, and productivity study on the Aedes aegypti most preferred containers in the City of Manaus, Amazonas, Brazil

The most preferred containers by Aedes aegypti were studied April and July (rainy and dry periods) in two Manaus neighbourhoods. In all, 2,700 premises and 13,912 containers were examined, most (87%) recorded outdoors. Out of the 13,100 inspected premises, only 1.6% showed to be positive for Aedes aegypti, summing up to 7,916 collected samples. Most frequently found containers outdoors in either neighbourhood regardless of rain or dry period were Bottles flasks and Storage, and indoors, Fixed, Flowerpots, and buckets. Productivity was estimated according to the number of premises and positive containers investigated, showing the actual container groups productivity. Considering both rainy and dry periods outdoors at Praça 14 the groups of Tyre, Flask, Bottle, Construction Equipment and Fixed, had the highest averages respectively. Construction Equipment and Flask groups were the most productive in Coroado in April. Flask, Construction Equipment and Storage groups stood out in July