Active phase-nulling of the self-mixing phase in a terahertz frequency quantum cascade laser

We demonstrate an active phase-nulling scheme for terahertz (THz) frequency quantum cascade lasers (QCLs) under optical feedback, by active electronic feedback control of the emission frequency. Using this scheme the frequency tuning rate of a THz QCL is characterised, with significantly reduced experimental complexity compared to alternative approaches. Furthermore, we demonstrate real-time displacement sensing of targets, overcoming the resolution limits imposed by quantisation in previously-implemented fringe counting methods. Our approach is readily applicable to high-frequency vibrometry and surface profiling of targets, as well as frequency-stabilisation schemes for THz QCLs

First observations with CONDOR, a 1.5 THz heterodyne receiver

The THz atmospheric windows centered at roughly 1.3 and 1.5~THz, contain numerous spectral lines of astronomical importance, including three high-J CO lines, the N+ line at 205 microns, and the ground transition of para-H2D+. The CO lines are tracers of hot (several 100K), dense gas; N+ is a cooling line of diffuse, ionized gas; the H2D+ line is a non-depleting tracer of cold (~20K), dense gas. As the THz lines benefit the study of diverse phenomena (from high-mass star-forming regions to the WIM to cold prestellar cores), we have built the CO N+ Deuterium Observations Receiver (CONDOR) to further explore the THz windows by ground-based observations. CONDOR was designed to be used at the Atacama Pathfinder EXperiment (APEX) and Stratospheric Observatory For Infrared Astronomy (SOFIA). CONDOR was installed at the APEX telescope and test observations were made to characterize the instrument. The combination of CONDOR on APEX successfully detected THz radiation from astronomical sources. CONDOR operated with typical Trec=1600K and spectral Allan variance times of 30s. CONDOR's first light observations of CO 13-12 emission from the hot core Orion FIR4 (= OMC1 South) revealed a narrow line with T(MB) = 210K and delta(V)=5.4km/s. A search for N+ emission from the ionization front of the Orion Bar resulted in a non-detection. The successful deployment of CONDOR at APEX demonstrates the potential for making observations at THz frequencies from ground-based facilities.Comment: 4 pages + list of objects, 3 figures, to be published in A&A special APEX issu

Photon Dominated Regions in NGC 3603

Aims: We aim at deriving the excitation conditions of the interstellar gas as well as the local FUV intensities in the molecular cloud surrounding NGC 3603 to get a coherent picture of how the gas is energized by the central stars. Methods: The NANTEN2-4m submillimeter antenna is used to map the [CI] 1-0, 2-1 and CO 4-3, 7-6 lines in a 2' x 2' region around the young OB cluster NGC 3603 YC. These data are combined with C18O 2-1 data, HIRES-processed IRAS 60 and 100 micron maps of the FIR continuum, and Spitzer/IRAC maps. Results: The NANTEN2 observations show the presence of two molecular clumps located south-east and south-west of the cluster and confirm the overall structure already found by previous CS and C18O observations. We find a slight position offset of the peak intensity of CO and [CI], and the atomic carbon appears to be further extended compared to the molecular material. We used the HIRES far-infrared dust data to derive a map of the FUV field heating the dust. We constrain the FUV field to values of \chi = 3 - 6 \times 10^3 in units of the Draine field across the clouds. Approximately 0.2 to 0.3 % of the total FUV energy is re-emitted in the [CII] 158 {\mu}m cooling line observed by ISO. Applying LTE and escape probability calculations, we derive temperatures (TMM1 = 43 K, TMM2 = 47 K), column densities (N(MM1) = 0.9 \times 10^22 cm^-2, N(MM2) = 2.5 \times 10^22 cm^-2) and densities (n(MM1) = 3 \times 10^3 cm^-3, n(MM2) = 10^3 -10^4 cm^-3) for the two observed molecular clumps MM1 and MM2. Conclusions: The cluster is strongly interacting with the ambient molecular cloud, governing its structure and physical conditions. A stability analysis shows the existence of gravitationally collapsing gas clumps which should lead to star formation. Embedded IR sources have already been observed in the outskirts of the molecular cloud and seem to support our conclusions.Comment: 13 pages, 10 figures, accepted for publication by A&

Phase-locking of a 2.7-THz quantum cascade laser to a mode-locked erbium-doped fibre laser

We demonstrate phase-locking of a 2.7-THz metalmetal waveguide quantum cascade laser (QCL) to an external microwave signal. The reference is the 15th harmonic, generated by a semiconductor superlattice nonlinear device, of a signal at 182 GHz, which itself is generated by a multiplier-chain (x2x3x2) from a microwave synthesizer at 15 GHz. Both laser and reference radiations are coupled into a hot electron bolometer mixer, resulting in a beat signal, which is fed into a phase-lock loop. Spectral analysis of the beat signal (see fig. 1) confirms that the QCL is phase locked. This result opens the possibility to extend heterodyne interferometers into the far-infrared range

Multi-environment association study highlights candidate genes for robust agronomic quantitative trait loci in a novel worldwide Capsicum core collection

Investigating crop diversity through genome-wide association studies (GWAS) on core collections helps in deciphering the genetic determinants of complex quantitative traits. Using the G2P-SOL project world collection of 10 038 wild and cultivated Capsicum accessions from 10 major genebanks, we assembled a core collection of 423 accessions representing the known genetic diversity. Since complex traits are often highly dependent upon environmental variables and genotype-by-environment (G × E) interactions, multi-environment GWAS with a 10 195-marker genotypic matrix were conducted on a highly diverse subset of 350 Capsicum annuum accessions, extensively phenotyped in up to six independent trials from five climatically differing countries. Environment-specific and multi-environment quantitative trait loci (QTLs) were detected for 23 diverse agronomic traits. We identified 97 candidate genes potentially implicated in 53 of the most robust and high-confidence QTLs for fruit flavor, color, size, and shape traits, and for plant productivity, vigor, and earliness traits. Investigating the genetic architecture of agronomic traits in this way will assist the development of genetic markers and pave the way for marker-assisted selection. The G2P-SOL pepper core collection will be available upon request as a unique and universal resource for further exploitation in future gene discovery and marker-assisted breeding efforts by the pepper community

A population genetic study of Vitis vinifera L. subsp. sylvestris Gmelin based on 3.000 individuals from 20 countries

Trabajo presentado en el 45th World Congress of Vine and Wine, celebrado en Dijon (Francia), del 14 al 18 de octubre de 2024Until the 19th century, the wild form of cultivated grapevines (vitis vinifera L. Subsp. Sylvestris gmelin, v. Sylvestris) was ubiquitous in many european and west asian regions. However, many factors like deforestation, the intensification of agriculture, or the introduction of several pests and pathogens decimated its presence in these growing sites, and natural populations are now mostly restricted to river-bank forests and creeks with specific soil and climate conditions. In fact, v. Sylvestris is now considered an endangered subspecies that is protected by law in many european countries to prevent its loss. In the past, there diversity was great and the different populations have adapted optimally to a variety of natural habitats. In view of the current and future climatic challenges, this diversity could prove to be the key to climate-resilient adaptation in breeding programs. Therefore, the protection and study of the remaining wild individuals is one key element for the preservation of our viticultural landscapes. In this study, we collected publicly available ssr data of v. Sylvestris genetic resources from 14 scientific publications, which was merged with the information obtained after the genetic characterization of 1460 new samples. As a result, we compiled the largest dataset for v. Sylvestris available to date, with approximately 3,000 unique genetic profiles collected from 20 countries of the natural growing range of this subspecies. After data curation and alleles¿ sizes standardisation according to reference varieties and allelic frequencies distribution patterns, this dataset was used to explore the genetic diversity and population structure of v. Sylvestris in a broad context. Preliminary results identified a series of clusters of genetically related samples, which mirrored their region of origin and reflected a major geographic substructuring within the explored sites. Additionally, we designed a core collection of v. Sylvestris genetic resources for targeted preservation across national borders. Altogether, this work provided a global update of the current status of this endangered subspecies, and provides relevant data for its proper management and conservation

The molecular environment of the massive star forming region NGC 2024: Multi CO transition analysis

NGC 2024, a sites of massive star formation, have complex internal structures caused by cal heating by young stars, outflows, and stellar winds. These complex cloud structures lead to intricate emission line shapes. The goal of this paper is to show that the complex line shapes of 12 CO lines in NGC 2024 can be explained consistently with a model, whose temperature and velocity structure are based on the well-established scenario of a PDR and the Blister model. We present velocity-resolved spectra of seven CO lines ranging from J=3 to J=13, and we combined these data with CO high-frequency data from the ISO satellite. We find that the bulk of the molecular cloud associated with NGC 2024 consists of warm (75 K) and dense (9e5 cm-3) gas. An additional hot (~ 300 K) component, located at the interface of the HII region and the molecular cloud, is needed to explain the emission of the high-J CO lines. Deep absorption notches indicate that very cold material (20 K) exists in front of the warm material, too. A temperature and column density structure consistent with those predicted by PDR models, combined with the velocity structure of a Blister model, appropriately describes the observed emission line profiles of this massive star forming region. This case study of NGC 2024 shows that, with physical insights into these complex regions and careful modeling, multi-line observations of CO can be used to derive detailed physical conditions in massive star forming regions.Comment: 10 pages, 5 figures, accepted by A&A for publicatio

Clumpy photon-dominated regions in Carina. I. [CI] and mid-J CO lines in two 4'x4' fields

The Carina region is an excellent astrophysical laboratory for studying the feedback mechanisms of newly born, very massive stars within their natal giant molecular clouds (GMCs) at only 2.35 kpc distance. We use a clumpy PDR model to analyse the observed intensities of atomic carbon and CO and to derive the excitation conditions of the gas. The NANTEN2-4m submillimeter telescope was used to map the [CI] 3P1-3P0, 3P2-3P1 and CO 4-3, 7-6 lines in two 4'x4' regions of Carina where molecular material interfaces with radiation from the massive star clusters. One region is the northern molecular cloud near the compact OB cluster Tr14, and the second region is in the molecular cloud south of etaCar and Tr16. These data were combined with 13CO SEST spectra, HIRES/IRAS 60um and 100um maps of the FIR continuum, and maps of 8um IRAC/Spitzer and MSX emission. We used the HIRES far-infrared dust data to create a map of the FUV field heating the gas. The northern region shows an FUV field of a few 1000 in Draine units while the field of the southern region is about a factor 10 weaker. We constructed models consisting of an ensemble of small spherically symmetric PDR clumps within the 38" beam (0.43pc), which follow canonical power-law mass and mass-size distributions. We find that an average local clump density of 2x10**5 cm-3 is needed to reproduce the observed line emission at two selected interface positions. Stationary, clumpy PDR models reproduce the observed cooling lines of atomic carbon and CO at two positions in the Carina Nebula.Comment: accepted by A&

Chromosome-scale genome assembly provides insights into rye biology, evolution and agronomic potential

Rye (Secale cereale L.) is an exceptionally climate-resilient cereal crop, used extensively to produce improved wheat varieties via introgressive hybridization and possessing the entire repertoire of genes necessary to enable hybrid breeding. Rye is allogamous and only recently domesticated, thus giving cultivated ryes access to a diverse and exploitable wild gene pool. To further enhance the agronomic potential of rye, we produced a chromosome-scale annotated assembly of the 7.9-gigabase rye genome and extensively validated its quality by using a suite of molecular genetic resources. We demonstrate applications of this resource with a broad range of investigations. We present findings on cultivated rye's incomplete genetic isolation from wild relatives, mechanisms of genome structural evolution, pathogen resistance, low-temperature tolerance, fertility control systems for hybrid breeding and the yield benefits of rye-wheat introgressions.Peer reviewe

Subcellular distribution and life cycle of Epstein-Barr virus in keratinocytes of oral hairy leukoplakia

The authors investigated the life cycle of Epstein-Barr virus (EBV) in keratinocytes of oral hairy leukoplakia by combining immunohistochemistry. DNA in situ hybridization, and lectin histochemistry with electron microscopy. Diffuse-staining components of the EBV early antigen complex (EA-D), EBV 150-kd capsid antigen (VCA), EBV membrane antigen (gp350/220), and double-stranded DNA were labeled with monoclonal antibodies. An EBV-DNA probe was used to locate EBV DNA. Wheat-germ agglutinin (WGA) was employed to distinguish Golgi-associated compartments. The authors found EBV proteins and EBV DNA only in keratinocytes with apparent viral assembly. In situ hybridization showed EBV DNA in free corelike material and in electron-dense cores of mature nucleocapsids. Monoclonal antibodies to nonspecific double-stranded DNA attached to the same structures and to marginated chromatin. Components of EA-D were dispersed throughout the nuclei but accumulated near condensed chromatin and in 'punched-out' regions of the chromatin. Epstein-Barr virus 150-kd capsid antigen was found only in the nuclei, where it appeared preferentially on mature nucleocapsids. As yet unexplained arrays of intranuclear particles that remained unlabeled with all EBV-specific probes reacted intensely with an antiserum against common papillomavirus antigen. Gp350/220 was detectable in various cellular membrane compartments and was highly concentrated on EBV envelopes in peripheral Golgi-associated secretory vesicles. It was less abundant on the extracellular EBV, indicating that viral membrane antigen partly dissociates from the mature virus. Combined lectin-binding histochemistry and electron microscopy demonstrated for the first time that EBV is processed in the Golgi apparatus, which eventually releases the virus by fusion with the plasma membrane. These results provide insight into the biologic events that occur during complete EBV replication in vivo